Clamping plate device for immunoblotting test

Abstract

The utility model discloses a clamping plate device for an immunoblotting test, which can improve the fitting degree between a polymeric membrane and a microfluidic channel cover through an improved connection scheme, and is longer in service life. Comprising a rectangular first clamping plate, a rectangular second clamping plate and a connecting bolt, the first clamping plate and the second clamping plate are provided with corresponding positioning through holes, the connecting bolt penetrates through the positioning through holes and is used for connecting the first clamping plate and the second clamping plate, and a plurality of swimming lanes for flowing samples are arranged in the middle of the inner side of the first clamping plate. A sample adding port and a sample sucking port which are communicated with two ends of the lane are formed in the outer side of the first clamping plate, a plurality of first magnets are embedded around the lane on the inner side surface of the first clamping plate, a plurality of second magnets are embedded on the inner side surface of the second clamping plate, and the first magnets and the second magnets are in one-to-one correspondence in position.

Description

Technical Field

[0001] The utility model relates to the field of experimental instruments, in particular to an immunoblotting test device. Background Art

[0002] Western blot (WB) is a protein identification technique widely used in the life sciences. Traditional WB consists of three main parts: electrophoresis, transfer, and detection. Electrophoresis is used to separate proteins according to their molecular weight. Transfer transfers the separated proteins to a polymer membrane. Detection utilizes antibody-specific recognition technology to detect the different proteins transferred to the polymer membrane. To reduce antibody usage while increasing detection throughput, a feasible approach is to cover the transferred polymer membrane with a microfluidic channel cap, perpendicular to the electrophoretic separation direction. Through the confinement of the microfluidic channel, multiple proteins separated in a single lane are simultaneously recognized and visualized by multiple antibodies.

[0003] A crucial aspect of implementing this technology is ensuring a good fit between the polymer membrane and the microfluidic channel cover. Otherwise, the antibody solution can easily seep from one channel into the other, causing cross-contamination. Existing micro high-throughput immunoblotting devices consist of two acrylic plates connected and fastened with bolts. However, the fit between the two plates is directly affected by the tightness of the threads, resulting in a less-than-ideal fit that decreases over time. Utility Model Content

[0004] The utility model aims to overcome the above-mentioned deficiencies of the immunoblotting test device in the prior art and provides a splint device for immunoblotting test. Through an improved connection scheme, the fit between the polymer membrane and the microfluidic channel cover can be improved and the service life is extended.

[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0006] The utility model discloses a splint device for immunoblotting test, comprising a rectangular first splint, a second splint and a connecting bolt, wherein the first splint and the second splint are provided with corresponding positioning through holes, and the connecting bolt passes through the positioning through holes to connect the first splint and the second splint, a plurality of swimming lanes for flowing samples are arranged in the middle part of the inner side of the first splint, a sample addition port and a sample suction port connecting the two ends of the swimming lanes are provided on the outer side of the first splint, a plurality of first magnets are embedded around the swimming lanes on the inner side surface of the first splint, and a plurality of second magnets are embedded on the inner side surface of the second splint, and the positions of the first magnets and the second magnets correspond one to one.

[0007] Preferably, the surface of the first magnet is flush with the inner side surface of the first clamping plate, and the surface of the second magnet is flush with the inner side surface of the second clamping plate.

[0008] Preferably, a positioning through hole is provided in the middle of one side of the short side of the first splint and the second splint and is located in the same straight line as the first magnet and the second magnet, and two positioning through holes are provided in the middle of one side of the long side of the first splint and the second splint and are located in the same straight line as the first magnet and the second magnet.

[0009] Preferably, the first plywood and the second plywood are made of transparent acrylic material.

[0010] Preferably, an LED light strip is provided on the outer side of the second splint.

[0011] Preferably, the second splint is provided with a blind hole at the position where the second magnet is set, and the second splint is provided with a sinking cavity on the back side of the blind hole. The second magnet is located in the blind hole, the depth of the blind hole is greater than the thickness of the second magnet, and a connecting rod is provided on the back of the second magnet, which passes through the bottom of the blind hole and is exposed on the surface of the sinking cavity. A lever self-locking handle is provided on the connecting rod. When the lever self-locking handle is perpendicular to the second splint, the connecting rod pulls the second magnet into the blind hole. When the lever self-locking handle is in contact with the second splint, the connecting rod pushes the second magnet out so that the surface of the second magnet is flush with the inner side surface of the second splint.

[0012] Therefore, the present invention has the following beneficial effects: (1) the magnetic attraction method is adopted to make the first clamping plate and the second clamping plate fit more tightly, avoiding the problem of gap or looseness of the connecting bolt threads causing the tightness of the fit to decrease, thereby extending the service life of the device; (2) the addition of LED light strips for supplementary lighting facilitates the observation of experimental results; (3) the lever self-locking handle is moved to a state perpendicular to the second clamping plate, so that the second magnet is retracted and separated from the first magnet, making it convenient to disassemble the first clamping plate and the second clamping plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a schematic structural diagram of the first splint of the present utility model.

[0014] Figure 2 This is a schematic structural diagram of the second splint of the present invention.

[0015] Figure 3 This is a cross-sectional view of the first and second splints when they are in use together.

[0016] Figure 4 This is a cross-sectional view of another embodiment of the first splint and the second splint when they are used in conjunction with each other.

[0017] Figure 5 It is a cross-sectional view of another embodiment when the first splint and the second splint are separated.

[0018] In the picture:

[0019] 1. First clamping plate; 101. First magnet; 102. Swimming lane;

[0020] 2. Second splint; 201. Second magnet; 202. LED light strip; 203. Blind hole; 204. Connecting rod; 205. Lever self-locking handle; 206. Lever fulcrum;

[0021] 3. Positioning through holes;

[0022] 4. Connecting bolts. DETAILED DESCRIPTION

[0023] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0024] like Figure 1 、 Figure 2 As shown, the utility model is a splint device for immunoblotting test, comprising a rectangular first splint 1, a second splint 2 and a connecting bolt 4, the first splint 1 and the second splint 2 are provided with corresponding positioning through holes 3, the connecting bolt 4 passes through the positioning through holes 3 to connect the first splint 1 and the second splint 2, a plurality of swimming lanes 102 for flowing samples are arranged in the middle part of the inner side of the first splint 1, a sample addition port and a sample suction port connecting the two ends of the swimming lane 102 are provided on the outer side of the first splint 1, a plurality of first magnets 101 are embedded around the swimming lane 102 on the inner side surface of the first splint 1, and a plurality of second magnets 201 are embedded on the inner side surface of the second splint 2, and the positions of the first magnet 101 and the second magnet 201 correspond one to one.

[0025] The surface of the first magnet 101 is flush with the inner side of the first clamping plate 1 , and the surface of the second magnet 201 is flush with the inner side of the second clamping plate 2 .

[0026] A positioning through hole 3 is provided in the middle of one side of the short side of the first clamping plate 1 and the second clamping plate 2 and is located on the same straight line as the first magnet 101 and the second magnet 201. Two positioning through holes 3 are provided in the middle of one side of the long side of the first clamping plate 1 and the second clamping plate 2 and are located on the same straight line as the first magnet 101 and the second magnet 201.

[0027] The first clamping plate 1 and the second clamping plate 2 are made of transparent acrylic material. An LED light strip 202 is provided on the outer side of the second clamping plate 2.

[0028] like Figure 3As shown, the clamping plate device for immunoblotting test in this embodiment adopts the magnetic attraction method of magnets, which can make the first clamping plate 1 and the second clamping plate 2 fit more tightly, avoid the problem of thread gap or looseness of the connecting bolt 4 causing the fitting tightness to decrease, and extend the service life of the device; add LED light strip 202 for supplementary lighting to facilitate observation of experimental results.

[0029] like Figure 4 、 Figure 5 As shown, in another optimized embodiment, the second splint 2 is provided with a blind hole 203 at the position where the second magnet 201 is set, and the second splint 2 is provided with a sinking cavity on the back side of the blind hole 203. The second magnet 201 is located in the blind hole 203, and the depth of the blind hole 203 is greater than the thickness of the second magnet 201. A connecting rod 204 is provided on the back of the second magnet 201, which passes through the bottom of the blind hole 203 and is exposed on the surface of the sinking cavity. A lever self-locking handle 205 is provided on the connecting rod 204.

[0030] The working principle of the lever self-locking handle 205 is that one end is hinged to the connecting rod 204, and a protruding lever fulcrum 206 is provided at the other end of the hinge position of the lever self-locking handle 205. When the lever self-locking handle 205 is perpendicular to the second splint 2, the lever fulcrum 206 presses against the second splint 2, so that the hinge point moves outward, so that the connecting rod 204 pulls the second magnet 201 into the blind hole 203, thereby separating the attracted first magnet 101 and the second magnet 201.

[0031] When the lever self-locking handle 205 is in contact with the second clamping plate 2, the lever fulcrum 206 no longer abuts the second clamping plate 2, the hinge point is reset, and the connecting rod 204 pushes out the second magnet 201, so that the surface of the second magnet 201 is flush with the inner surface of the second clamping plate 2. By moving the lever self-locking handle 205 to a position perpendicular to the second clamping plate 2, the second magnet 201 is retracted and separated from the first magnet 101, making it easier to disassemble the first clamping plate 1 and the second clamping plate 2.

Claims

1. A clamping plate device for immunoblotting test, comprising a rectangular first clamping plate, a second clamping plate, and a connecting bolt, wherein the first clamping plate and the second clamping plate are provided with corresponding positioning through holes, and the connecting bolt passes through the positioning through holes to connect the first clamping plate and the second clamping plate, characterized in that: Several swimming lanes for flowing samples are arranged in the middle of the inner side of the first splint, and a sample addition port and a sample suction port connecting the two ends of the swimming lanes are provided on the outer side of the first splint. Several first magnets are embedded around the swimming lanes on the inner side of the first splint, and several second magnets are embedded on the inner side of the second splint, and the positions of the first magnets and the second magnets correspond one to one.

2. The immunoblotting test splint device according to claim 1, wherein: The surface of the first magnet is flush with the inner side of the first clamping plate, and the surface of the second magnet is flush with the inner side of the second clamping plate.

3. The splint device for immunoblotting test according to claim 1, wherein: A positioning through hole is provided in the middle of one side of the short side of the first splint and the second splint and is located on the same straight line as the first magnet and the second magnet. Two positioning through holes are provided in the middle of one side of the long side of the first splint and the second splint and are located on the same straight line as the first magnet and the second magnet.

4. The splint device for immunoblotting test according to claim 1, wherein: The first plywood and the second plywood are made of transparent acrylic material.

5. The splint device for immunoblotting test according to claim 1, wherein: An LED light strip is provided on the outer side of the second splint.

6. The splint device for immunoblotting test according to claim 2, wherein: The second splint is provided with a blind hole at the position where the second magnet is set, and the second splint is provided with a sinking cavity on the back side of the blind hole. The second magnet is located in the blind hole, and the depth of the blind hole is greater than the thickness of the second magnet. A connecting rod is provided on the back of the second magnet, which passes through the bottom of the blind hole and is exposed on the surface of the sinking cavity. A lever self-locking handle is provided on the connecting rod. When the lever self-locking handle is perpendicular to the second splint, the connecting rod pulls the second magnet into the blind hole. When the lever self-locking handle is in contact with the second splint, the connecting rod pushes the second magnet out so that the surface of the second magnet is flush with the inner side surface of the second splint.

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