Laser-assisted transparent belt punching system
Through the optimized design of the laser emission module, guide device and microscope assembly, the problems of large space requirements and low precision caused by traditional arc guide rails were solved, and high-precision laser transparent belt drilling was achieved.
Patent Information
- Application Number
- CN202422524286.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-18
- Publication Date
- 2025-09-26
- Estimated Expiration
- 2034-10-18
AI Technical Summary
Traditional laser transparent tape punching machines use arc guide rails, which result in large space requirements, high processing difficulty, and low movement accuracy, affecting the punching accuracy.
The laser emission module, guide device and microscope assembly are connected to the biological microscope through an electric slide rail. The concave reflector and secondary reflector are used to guide the light beam to achieve two-dimensional movement of the laser emission module, and high-precision focusing is achieved in combination with the objective lens.
The drilling accuracy is improved, the space occupation and adjustment cost are reduced, and high-precision laser drilling is achieved.
Smart Images
Figure CN223382801U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of medical equipment, and in particular relates to a laser-assisted transparent band punching system. Background Art
[0002] As demand for assisted reproductive technology continues to grow, embryo culture technology has garnered increasing attention. The laser-assisted zona pellucida perforation system uses lasers to ablate the zona pellucida, effectively increasing embryo implantation rates and shortening the time it takes for patients to achieve successful conception.
[0003] Depending on the embryo sample, different areas of the zona pellucida often need to be ablated. Therefore, the device often requires a control module to achieve precise infrared laser ablation. Traditional laser zona pellucida perforators typically use curved guides in their control modules, which move the indicator laser and ablation laser emitters, adjusting the laser beam direction to achieve ablation at different locations in the zona pellucida.
[0004] Using an arc guide to guide the laser beam of the zona pellucida laser perforator requires considerable space to adjust its position on the sample. The corresponding arc guide and the corresponding equipment module are also subject to significant limitations and difficulties in their processing and layout. Furthermore, the control scheme for the movement of the indicator laser and the ablation laser emitter is also complex relative to adjusting the laser focus position on the embryonic zona pellucida sample. Failure to meet the arc guide's specifications and space requirements will result in reduced movement accuracy and, consequently, reduced sample perforation precision. Utility Model Content
[0005] The present invention provides a laser-assisted transparent tape punching system that can address the adverse effects of using arc guide rails for laser transparent tape punching in related technologies and improve punching accuracy. The technical solution is as follows:
[0006] The embodiment of the utility model provides a laser-assisted transparent tape punching system, comprising: a laser emission module, a guide device and a microscope assembly,
[0007] The laser emission module is arranged beside the microscope assembly, and includes a laser light source and a fiber collimator connected in sequence. The output end of the laser emission module faces the guide device, and the laser emission module is configured to be able to move two-dimensionally relative to the light incident side of the guide device;
[0008] The guiding device includes a concave reflecting mirror, which is arranged at an angle relative to the light output direction of the optical fiber collimator and is used to guide the light beam emitted by the laser emission module to irradiate the incident end of the microscope assembly;
[0009] The microscope assembly includes a secondary reflector, an objective lens and a culture dish arranged in sequence. The secondary reflector is provided at the incident end of the microscope assembly and is used to reflect the light beam incident through the guide device to the objective lens and focus it on the culture dish.
[0010] Optionally, the concave reflector is arranged at 45° relative to the light output direction of the optical fiber collimator, and the secondary reflector is arranged at 90° to the concave reflector.
[0011] Optionally, the guiding device also includes a beam splitter, which is arranged below the output end of the laser emission module and is located between the concave reflector and the secondary reflector in the horizontal direction. The beam splitter is arranged at 45° relative to the light output direction of the fiber optic collimator, the concave reflector is parallel to the light output direction of the fiber optic collimator, and the secondary reflector is parallel to the beam splitter.
[0012] Optionally, the guiding device also includes a conical lens, which is arranged between the incident end of the microscope assembly and the output end of the laser emission module, the conical surface of the conical lens faces the microscope assembly, and the plane of the conical lens faces the laser emission module and is perpendicular to the light output direction of the optical fiber collimator.
[0013] Optionally, the laser light source includes an indicator laser light source and an infrared ablation laser light source, and the laser emission module further includes a fiber coupler, and the indicator laser light source and the infrared ablation laser light source are connected to the fiber collimator through the fiber coupler.
[0014] Optionally, the wavelength range of the laser emitted by the indicator laser light source is 630-660 nm, and the wavelength range of the laser emitted by the infrared ablation laser light source is 1450-1500 nm.
[0015] Optionally, the secondary reflector is a dichroic mirror.
[0016] The beneficial effects of the technical solution provided by the embodiment of the utility model include at least:
[0017] The laser-assisted transparent band punching system provided by the embodiment of the present invention optimizes the guiding mode and setting form of the laser emission module with a mobile guiding function, and is connected to the microscope component of the biological microscope structure as the main body through an electric slide rail or a slide structure to achieve two-dimensional movement of the light incident side relative to the guiding device. It uses a guiding device mainly composed of a concave reflector to reflect and guide the light beam, and cooperates with the secondary reflector and objective lens in the microscope component to reflect and focus the light beam on the culture dish to perform directional ablation of the sample. It only needs to drive the laser emission module to perform a simple lateral two-dimensional movement to perform high-precision angular guidance adjustment on the light beam to achieve punching at different positions on the sample. Compared with the traditional arc guide rail solution, it has higher movement adjustment accuracy for the laser emission end such as the laser light source, and occupies and adjusts the space required, with lower setting cost. It can solve the adverse effects caused by the use of arc guide rails for laser transparent band punching operations in related technologies and improve punching accuracy. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0019] Figure 1 This is a schematic structural diagram of a laser-assisted transparent tape punching system provided by an embodiment of the present utility model;
[0020] Figure 2 This is a schematic diagram of laser drilling at different positions of a sample provided by an embodiment of the present utility model;
[0021] Figure 3 This is a schematic structural diagram of a second laser-assisted transparent tape punching system provided by an embodiment of the present utility model;
[0022] Figure 4 This is a structural diagram of a third laser-assisted transparent tape punching system provided in an embodiment of the present utility model.
[0023] In the picture:
[0024] 1-Laser emission module; 2-Guide device; 3-Microscope assembly; 11-Laser light source; 12-Fiber collimator; 13-Fiber coupler; 21-Concave reflector; 22-Beam splitter; 23-Axis lens; 31-Secondary reflector; 32-Objective lens; 33-Cultivar dish; 111-Indicator laser light source; 112-Infrared ablation laser light source. DETAILED DESCRIPTION
[0025] In order to make the purpose, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.
[0026] Figure 1 This is a schematic structural diagram of a laser-assisted transparent tape punching system provided by an embodiment of the present utility model; Figure 2 This is a schematic diagram of laser drilling at different positions of a sample provided by an embodiment of the present utility model; Figure 3 This is a schematic structural diagram of a second laser-assisted transparent tape punching system provided by an embodiment of the present utility model; Figure 4 This is a schematic diagram of the structure of the third laser-assisted transparent band punching system provided by the embodiment of the present utility model. Figures 1 to 4 As shown, an embodiment of the present invention provides a laser-assisted transparent tape punching system, comprising: a laser emission module 1, a guide device 2 and a microscope assembly 3.
[0027] The laser emission module 1 is positioned adjacent to the microscope assembly 3 and comprises a laser light source 11 and a fiber collimator 12, connected in sequence. The output end of the laser emission module 1 faces the guide device 2, and the laser emission module 1 is configured to move two-dimensionally relative to the light-entering side of the guide device 2.
[0028] The guiding device 2 includes a concave reflector 21 . The concave reflector 21 is arranged at an angle relative to the light emitting direction of the optical fiber collimator 12 , and is used to guide the light beam emitted by the laser emission module 1 to illuminate the incident end of the microscope assembly 3 .
[0029] The microscope assembly 3 includes a secondary reflector 31, an objective lens 32 and a culture dish 33. The secondary reflector 31 is provided at the incident end of the microscope assembly 3 and is used to reflect the light beam incident through the guide device 2 to the objective lens 32 and focus it on the culture dish 33.
[0030] In an embodiment of the present invention, the laser emission module 1 and the guide device 2 are structures independent of the microscope assembly 3, wherein the microscope assembly 3 can be a unit integrated into a biological microscope in a laboratory. When the embryo zona pellucida punching operation is not performed, it can itself serve as a part of the biological microscope, and the experimenter can observe the sample in the culture dish 33 through the eyepiece and other structures above the culture dish 33. The laser emission module 1 can be connected to the biological microscope as the main body through an electric slide rail or a slide structure, and the guide device 2 can be fixedly mounted using a corresponding bracket structure to ensure that the laser emission module 1 can move horizontally in two dimensions relative to the light incident side of the guide device 2 under electric drive. Furthermore, the laser light source 11 inside the laser emission module 1 includes an indicator laser light source 111 and an infrared ablation laser light source 112. A fiber optic coupler 13 is also provided in the laser emission module 1. The indicator laser light source 111 and the infrared ablation laser light source 112 are connected to the fiber collimator 12 through the fiber optic coupler 13. The laser wavelength emitted by the indicator laser light source 111 is 650nm, and the laser wavelength emitted by the infrared ablation laser light source 112 is 1480nm. When the laser light source 11 is in operation, the indicator laser light source 111 first starts to emit invisible light with a wavelength of 650nm as the indicator laser to indicate the position where the ablation laser will eventually hit the sample in the culture dish 33. Then, the indicator laser light source 111 is turned off, and the infrared ablation laser light source 112 is started to emit ablation laser with a wavelength of 1480nm. After being coupled with the indicator laser by the fiber coupler 13 to achieve a unified light output path, it is then collimated by the fiber collimator 12 to form an output beam, which is conducive to propagation in space.
[0031] In the first embodiment of the present invention, the laser emission module 1 is arranged beside the microscope assembly 3 in the horizontal direction, and the light emitting direction of its fiber optic collimator 12 is downward in the vertical direction. The concave reflector 21 serving as the guide device 2 is arranged directly below the light emitting direction of the fiber optic collimator 12, and its arc concave side faces the laser emission module 1, and the whole is arranged at 45° relative to the light emitting direction of the fiber optic collimator 12. Correspondingly, the secondary reflector 31 located at the incident end of the microscope assembly 3 is arranged at 90° to the concave reflector 21. When the entire laser-assisted transparent band punching system is in the initial working condition, the light beam emitted by the laser emission module 1 hits the concave reflector 21 and is arranged along the concave reflector 21. Figure 1 After the straight optical path in the microscope assembly 3 is reflected to the incident end, it is reflected to the objective lens 32 by the secondary reflector 31 and focused on the culture dish 33, so as to achieve directional ablation of the first punching position of the sample in the culture dish 33. Furthermore, by driving the laser emission module 1 to move horizontally in two dimensions relative to the concave reflector 21, the light beam is made to hit different positions of the concave reflector 21 and move along the concave reflector 21. Figure 1The dotted light path in the middle is reflected at different angles to the incident end of the microscope assembly 3, and then reflected by the secondary reflector 31 to the objective lens 32 and focused on the culture dish 33, so as to reach the second punching position of the dotted light path on the left side on the culture dish 33, or the third punching position of the dotted light path on the right side on the culture dish 33, etc.
[0032] The laser-assisted transparent band punching system provided by the embodiment of the present invention optimizes the guiding mode and setting form of the laser emission module 1 with a mobile guiding function, and is connected to the microscope assembly 3 of the biological microscope structure as the main body through an electric slide rail or slide structure to achieve two-dimensional movement on the light incident side relative to the guide device 2. It uses the guide device 2 with a concave reflector 21 as the main body to reflect and guide the light beam, and cooperates with the secondary reflector 31 and objective lens 32 in the microscope assembly 3 to reflect and focus the light beam on the culture dish 33 to perform directional ablation of the sample. It only needs to drive the laser emission module 1 to perform simple lateral two-dimensional movement to perform high-precision angular guidance adjustment of the light beam to achieve punching at different positions on the sample. Compared with the traditional arc guide rail solution, it has higher movement adjustment accuracy for the laser emission end such as the laser light source 11, and the space required for occupation and adjustment is smaller, and the setup cost is lower. It can solve the adverse effects caused by the use of arc guide rails for laser transparent band punching operations in related technologies and improve the punching accuracy.
[0033] It should be noted that in the embodiment of the present invention, the rear focal plane of the concave reflector 21 and the rear focal plane of the objective lens 32 coincide with each other, ensuring that the light spots at different positions on the concave reflector 21 are all converged onto the rear focal plane of the objective lens 32, and can ensure that when the two-dimensional slide and other structures drive the laser emission module 1 to move, the indicator laser and the infrared laser will not deviate beyond the light aperture of the objective lens 32.
[0034] Further, for the schematic diagram of the laser entering the microscope assembly 3 drilling holes at different positions of the sample, refer to Figure 2 , different punching positions require light of different angles to enter the objective lens 32 at the rear focal plane of the objective lens 32, such as Figure 2 The solid line is the first punching position, and the dotted line is the second punching position. The parallel light beam at the first punching position passes through the objective lens 32 in the horizontal direction and is focused on the sample surface of the culture dish 33 to achieve punching. After the laser emission module 1 drives the laser to move two-dimensionally, the switching between the first punching position and the second punching position is achieved through reflection by the guide device 2. The parallel light at the second punching position forms a certain angle θ with the optical axis and is focused at a position with a height h at the center of the sample surface after passing through the objective lens 32. The corresponding relationship is: ,
[0035] Where f is the focal length of the objective lens, and θ is the angle between the parallel light beam and the optical axis.
[0036] Optionally, the guiding device 2 also includes a beam splitter 22, which is arranged below the output end of the laser emission module 1 and is located between the concave reflector 21 and the secondary reflector 31 in the horizontal direction. The beam splitter 22 is arranged at 45° relative to the light emitting direction of the fiber optic collimator 12, the concave reflector 21 is parallel to the light emitting direction of the fiber optic collimator 12, and the secondary reflector 31 is parallel to the beam splitter 22.
[0037] For example, in the second embodiment of the present invention, based on the first embodiment, a beam splitter 22 and a concave reflector 21 are used to jointly reflect the light beam emitted by the laser emission module 1. The position of the concave reflector 21 is shifted horizontally away from the incident end of the microscope assembly 3, and its setting angle is changed so that it is parallel to the light output direction of the fiber collimator 12, that is, arranged vertically. Accordingly, the beam splitter 22 is arranged directly below the output end of the laser emission module 1 and is arranged at a 45° angle relative to the light output direction of the fiber collimator 12. The light beam emitted from the output end of the laser emission module 1 is reflected by the beam splitter 22 and then incident on the concave reflector 21 in a direction perpendicular to the concave reflector 21. After being reflected by the concave reflector 21, it hits the beam splitter 22 again. The beam splitter 22 can partially transmit and partially reflect the laser beams with wavelengths of 650nm and 1480nm. The reflected laser beam passes through the beam splitter 22 and hits the incident end of the microscope assembly 3. It is then reflected by the secondary reflector 31 to the objective lens 32 and focused on the culture dish 33 to achieve directional ablation of the first punching position of the sample in the culture dish 33. Figure 3 Correspondingly, by driving the laser emission module 1 to perform a simple lateral two-dimensional movement, the beam can be adjusted with high precision to achieve drilling at different positions on the sample. Figure 3 The dotted light path in FIG. By adding a beam splitter 22 to cooperate with the concave reflector 21 to reflect and guide the laser, the concave reflector 21 does not need to be placed at a specific angle. The light beam emitted by the laser emission module 1 can be reflected by the beam splitter 22 and then hit the arc concave surface of the concave reflector 21 in a vertical direction for angle adjustment and reflection. Compared with the first embodiment, the light spot that is finally focused on the culture dish 33 after being reflected by the concave reflector 21 placed at a specific angle is usually elliptical in shape. In the second embodiment, the light spot that is finally focused on the culture dish 33 will be a more regular circle, and its light spot energy distribution will be more uniform, thereby improving the consistency and accuracy of the transparent zone punching operation in the sample position.
[0038] Optionally, the guiding device 2 also includes a conical lens 23, which is arranged between the incident end of the microscope assembly 3 and the output end of the laser emission module 1, the conical surface of the conical lens 23 faces the microscope assembly 3, and the plane of the conical lens 23 faces the laser emission module 1 and is perpendicular to the light output direction of the optical fiber collimator 12.
[0039] For example, refer to Figure 4 In the third embodiment of the present invention, the configuration of the laser emission module 1 and the type of the accompanying guide device 2 are further optimized and modified. By placing the laser emission module 1 horizontally, with its output end arranged horizontally, and configuring the guide device 2 between the laser emission module 1 and the microscope assembly 3 as an axicon 23, the collimated light beam emitted by the laser emission module 1, upon striking the plane of the axicon 23, passes through and refracts through the conical surface to the incident end of the microscope assembly 3, ultimately focusing on the culture dish 33 for targeted ablation. Accordingly, the laser emission module 1 can be connected to the biological microscope as the main body via an electric slide or slide structure. The drive direction is then changed to the longitudinal direction. By driving the laser emission module 1 to rise or fall, the beam is guided and the drilling position is changed. Compared to the first two embodiments, this configuration of the laser emission module 1, by placing it horizontally, further reduces the longitudinal space occupied by the laser emission module 1 when combined with the biological microscope as the main body, improving overall adaptability and practicality.
[0040] Optionally, in the third embodiment, the aconical lens 23 may be replaced by a convex lens, as long as accurate refraction of the collimated light beam output by the laser emitting module 1 can be achieved.
[0041] Optionally, the secondary reflector 31 is a dichroic mirror. For example, in an embodiment of the present invention, a dichroic mirror is used as the secondary reflector 31. While ensuring that light beams with wavelengths of 650 nm and 1480 nm can be reflected onto the sample on the culture dish 33, the light beam from the biological microscope's overhead illumination can be imaged through the dichroic mirror.
[0042] Furthermore, in another possible implementation method of the embodiment of the present invention, the laser emission module 1 is driven to move two-dimensionally relative to the light incident side of the guide device 2. In addition to driving the laser emission module 1 to move as a whole through structures such as electric slide rails or slide structures, the optical path can also be adjusted by adjusting the position or angle of the concave reflector 21 or the conical lens 23 to achieve ablation at different positions on the final sample.
[0043]
[0044] Unless otherwise defined, the technical or scientific terms used herein shall have the ordinary meaning understood by persons of ordinary skill in the field to which the present invention belongs. The terms "first", "second" and similar words used in the specification and claims of the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, terms such as "one" or "a" do not indicate a limit on quantity, but rather indicate the presence of at least one. Terms such as "include" or "comprising" mean that the elements or objects appearing before "include" or "comprising" are extremely equivalent to the elements or objects listed after "include" or "comprising", and do not exclude other elements or objects. Terms such as "connected" or "connected" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. Terms such as "upper", "lower", "left", and "right" are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.
[0045] The above description is only an optional embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the scope of protection of the present invention.
Claims
1. A laser-assisted transparent tape punching system, characterized in that: include: Laser emission module (1), guide device (2) and microscope assembly (3), The laser emission module (1) is arranged beside the microscope assembly (3), and comprises a laser light source (11) and a fiber collimator (12) connected in sequence, the output end of the laser emission module (1) faces the guide device (2), and the laser emission module (1) is configured to be able to move two-dimensionally relative to the light incident side of the guide device (2); The guiding device (2) includes a concave reflector (21), which is arranged at an angle relative to the light output direction of the optical fiber collimator (12) and is used to guide the light beam emitted by the laser emission module (1) so as to irradiate the incident end of the microscope assembly (3); The microscope assembly (3) comprises a secondary reflector (31), an objective lens (32), and a culture dish (33) arranged in sequence. The secondary reflector (31) is provided at the incident end of the microscope assembly (3) and is used to reflect the light beam incident through the guide device (2) to the objective lens (32) and focus the light beam on the culture dish (33).
2. The laser-assisted transparent band punching system according to claim 1, characterized in that: The concave reflector (21) is arranged at 45 degrees relative to the light emitting direction of the optical fiber collimator (12), and the secondary reflector (31) is arranged at 90 degrees to the concave reflector (21).
3. The laser-assisted transparent band punching system according to claim 1, characterized in that: The guiding device (2) further includes a beam splitter (22), which is arranged below the output end of the laser emission module (1) and is located between the concave reflector (21) and the secondary reflector (31) in the horizontal direction. The beam splitter (22) is arranged at 45 degrees relative to the light output direction of the optical fiber collimator (12), the concave reflector (21) is parallel to the light output direction of the optical fiber collimator (12), and the secondary reflector (31) is parallel to the beam splitter (22).
4. The laser-assisted transparent band punching system according to claim 1, characterized in that: The guide device (2) further includes a conical lens (23), which is arranged between the incident end of the microscope assembly (3) and the output end of the laser emission module (1), the conical surface of the conical lens (23) faces the microscope assembly (3), and the plane of the conical lens (23) faces the laser emission module (1) and is perpendicular to the light output direction of the optical fiber collimator (12).
5. The laser-assisted transparent tape punching system according to any one of claims 1 to 4, characterized in that: The laser light source (11) comprises an indicator laser light source (111) and an infrared ablation laser light source (112); the laser emission module (1) further comprises an optical fiber coupler (13); the indicator laser light source (111) and the infrared ablation laser light source (112) are connected to the optical fiber collimator (12) via the optical fiber coupler (13).
6. The laser-assisted transparent tape punching system according to claim 5, characterized in that: The wavelength range of the laser light emitted by the indicator laser light source (111) is 630-660 nm, and the wavelength range of the laser light emitted by the infrared ablation laser light source (112) is 1450-1500 nm.
7. The laser-assisted transparent tape punching system according to any one of claims 1 to 4, characterized in that: The secondary reflector (31) is a dichroic mirror.