Automatic liquid separator pipeline

Abstract

The utility model discloses an automatic liquid separator pipeline, and belongs to the technical field of automatic pipetting devices. The technical problem that a pipeline in an existing organ-like automatic pipetting device is prone to being blocked is solved. The automatic liquid separator pipeline comprises a nozzle unit and a pipeline unit, the nozzle unit comprises a nozzle tip part, a buckle part and a connecting part which are connected in sequence; the liquid spraying end of the nozzle tip part points to the position of the culture pore plate; the nozzle tip part comprises a hollow cylindrical section pipe body and a hollow circular truncated cone section sharp nozzle which are integrally formed; the hollow cylindrical section tube body is used for being connected with the buckle part, and the hollow circular truncated cone section sharp nozzle is used for spraying liquid for sample adding. The pipeline unit comprises a pipette, one end of the pipette is connected with the organoid culture medium through an automatic pipetting device, and the other end of the pipette is fixedly connected with the connecting part. According to the utility model, the organoid can quickly pass through the pipette and the tip part of the nozzle, so that the organoid is prevented from blocking a pipeline.

Description

Technical Field

[0001] The utility model relates to the technical field of automated liquid transfer devices, in particular to an automated liquid dispenser pipeline. Background Art

[0002] Organoids are organ models that utilize matrix gel or other biomaterials to culture primary tissues in three dimensions in vitro. Due to their stable phenotypic and genetic characteristics, they can demonstrate the authentic structure and key functions of human organs. As a revolutionary model for pharmaceutical R&D, organoids have been widely used in basic research. However, their industrialization still faces numerous challenges, including poor standardization and reproducible results. Therefore, it is necessary to establish an automated organoid workstation to comprehensively build a high-throughput, intelligent, automated, and integrated organoid drug sensitivity testing and evaluation system, thus achieving standardization and systematization of the organoid drug sensitivity screening platform. Due to the heavy reliance of matrix gel on temperature for coagulation, as well as the influence of matrix gel concentration, viscosity, and organoid size, the pipelines in the automated equipment often become clogged and foreign matter remains, which greatly hinders the development of automated platforms for standardized culture and high-throughput drug / drug sensitivity screening.

[0003] Currently, automated pipetting equipment on the market primarily consists of two types: pipette lines and pipette tips. While pipette tips can address issues like clogging, they lack the flexibility to select culture wells and cannot perform micro-laying on 384-well plates, making them incapable of high-precision automated organoid culture. Automated pipetting devices using pipette lines have poor adaptability, with one type of line typically only compatible with one specific pipette. Further development is required to meet the requirements of automated organoid culture. Utility Model Content

[0004] In view of the above analysis, the present invention aims to provide an automated dispenser pipeline to solve the problems of pipeline blockage, foreign matter residue and incomplete cleaning in existing organoid automated pipetting devices.

[0005] The purpose of this utility model is mainly achieved through the following technical solutions:

[0006] The utility model provides an automatic liquid dispenser pipeline, which includes a nozzle unit and a pipeline unit;

[0007] The nozzle unit includes a nozzle tip portion, a buckle portion, and a connecting portion connected in sequence; the nozzle tip portion includes an integrally formed hollow cylindrical section body and a hollow frustum section nozzle; the hollow cylindrical section body is used to connect to the buckle portion, and the hollow frustum section nozzle is used to spray liquid for sample addition;

[0008] The pipeline unit includes a pipette, one end of which is connected to the organoid culture medium via an automated pipetting device, and the other end is fixedly connected to the connecting part.

[0009] In one possible design, the hollow frustum segment tip includes a tip end and a frustum base end;

[0010] The outer wall diameter of the truncated cone base end is larger than the outer wall diameter of the pointed end; the outer wall diameter of the truncated cone base end of the hollow truncated cone section pointed end is equal to the outer wall diameter of the hollow cylindrical section tube body.

[0011] In one possible design, the length of the tip of the hollow frustum section is 2.90 mm;

[0012] The inner wall diameter of the tip end of the hollow frustum segment tip is 0.50 mm, the outer wall diameter is 1 mm, and the outer wall diameter of the frustum base end of the hollow frustum segment tip is 2 mm.

[0013] In one possible design, the length of the hollow cylindrical section is 3.70 mm, the inner wall diameter is 1.00 mm, and the outer wall diameter is 2.00 mm.

[0014] In one possible design, the length of the pipette is 500 mm; the inner diameter of the pipette lumen is set to 1.30 mm;

[0015] The pipette is made of medical silicone hose.

[0016] In one possible design, the snap-fit ​​part includes a fixing block; a fixing groove is provided in the automated pipetting device, and the fixing block can be embedded in the fixing groove of the automated pipetting device; the snap-fit ​​part is used to fix the nozzle tip part as a whole at a specified position of the automated pipetting device.

[0017] In a possible design, the fixing block is a rectangular fixing block, the fixing slot in the automated pipetting device is a rectangular groove, and the size of the rectangular fixing block matches the size of the rectangular groove in the automated pipetting device.

[0018] In a possible design, the connecting portion includes a trapezoidal transition connecting tube; the outer wall diameter of the trapezoidal transition connecting tube is larger than the outer wall diameter of the pipette;

[0019] The trapezoidal transition connecting tube can be inserted into the pipette and form a sealed structure therewith.

[0020] In one possible design, the trapezoidal transition connecting pipe includes a first cylindrical section, an intermediate truncated cone section, and a second cylindrical section which are sequentially connected and all have hollow structures;

[0021] The first cylindrical section is fixedly connected to the buckle part, the outer wall diameter of the first cylindrical section is larger than the outer wall diameter of the second cylindrical section, and the outer wall diameters of the first cylindrical section, the middle frustum section and the second cylindrical section are all larger than the outer wall diameter of the pipette.

[0022] In one possible design, the middle frustum section includes a first circular bottom surface and a second circular bottom surface; wherein, the first circular bottom surface is adjacent to the first cylindrical section and the outer wall diameter and inner wall diameter of the two are equal; the second circular bottom surface is adjacent to the second cylindrical section and the outer wall diameter and inner wall diameter of the two are equal.

[0023] Compared with the existing technology, the present invention can achieve at least one of the following beneficial effects:

[0024] (1) By setting the nozzle tip part to a hollow cylindrical tube body and a hollow truncated cone tip structure, it can ensure that the organoids can pass through the automated dispenser pipeline smoothly and avoid organoids blocking the pipeline.

[0025] (2) The utility model sets the outer wall diameter of the trapezoidal transition connecting tube to be larger than the outer wall diameter of the pipette, which can ensure that after the connecting part is inserted into the pipette, a closed structure is formed between the nozzle tip part and the pipette.

[0026] (3) The utility model sets the connecting part as a trapezoidal transition connecting tube, which helps to insert the pipette more smoothly.

[0027] (4) The pipette of the utility model is a medical silicone hose, which is safer to use and more in line with clinical requirements; the medical silicone hose is easier to disinfect and can always be kept sterile and clean; the medical silicone hose is highly flexible and can be bent flexibly, and will not cause pipeline blockage and liquid retention due to bending.

[0028] In the present invention, the above-mentioned technical solutions can be combined with each other to achieve more preferred combination solutions. Other features and advantages of the present invention will be described in the subsequent description, and some advantages will become apparent from the description or be understood through practice of the present invention. The objectives and other advantages of the present invention can be achieved and obtained through the embodiments of the description and the contents particularly pointed out in the drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The accompanying drawings are only used for the purpose of illustrating specific embodiments and are not to be considered as limiting the present invention. Throughout the accompanying drawings, the same reference symbols denote the same components.

[0030] Figure 1 This is a schematic diagram of the structure of the nozzle unit of the automatic liquid dispenser pipeline of the utility model. Figure 1 ;

[0031] Figure 2This is a schematic diagram of the structure of the nozzle unit of the automatic liquid dispenser pipeline of the utility model. Figure 2 ;

[0032] Figure 3 This is a schematic diagram of the structure of the nozzle unit of the automatic liquid dispenser pipeline of the utility model. Figure 3 ;

[0033] Figure 4 This is a schematic diagram of the structure of the pipette of the automatic liquid dispenser pipeline of the utility model;

[0034] Figure 5 This is a vertical cross-sectional view of a pipette in the pipeline of the automated dispenser of the present invention.

[0035] Reference numerals:

[0036] 1-Hollow conical section tip; 2-Hollow cylindrical section tube body; 3-Snap-on part; 4-First cylindrical section; 5-Middle conical section; 6-Second cylindrical section; 7-Nozzle tip; 8-Pipette. DETAILED DESCRIPTION

[0037] The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, wherein the accompanying drawings constitute a part of the present invention and are used together with the embodiments of the present invention to illustrate the principles of the present invention, and are not used to limit the scope of the present invention.

[0038] The utility model provides an automatic liquid dispenser pipeline, such as Figures 1 to 5 As shown, the automated dispenser pipeline includes a nozzle unit 7 and a pipeline unit; the nozzle unit 7 includes a nozzle tip part, a snap part 3 and a connecting part connected in sequence; the nozzle tip part includes an integrally formed hollow cylindrical section tube body 2 and a hollow frustum section tip 1; the hollow cylindrical section tube body 2 is used to connect with the snap part 3, and the hollow frustum section tip 1 is used to spray liquid for sampling; the pipeline unit includes a pipette 8, one end of the pipette 8 is connected to the organoid culture medium through an automated pipetting device, and the other end is fixedly connected to the connecting part.

[0039] Specifically, the automated liquid dispenser pipeline of the present invention includes two parts: a nozzle unit 7 and a pipeline unit; wherein the pipeline unit includes a pipette 8; the nozzle unit 7 includes a nozzle tip portion, a snap portion 3 and a connecting portion connected in sequence; wherein the snap portion 3 is used to connect the nozzle tip portion and the connecting portion, and the snap portion 3 can be fixed at a designated position on the automated liquid dispensing device; one end of the connecting portion is fixedly connected to the snap portion 3, and the other end can be inserted into the pipette 8, so that the pipette 8 and the nozzle tip portion of the nozzle unit 7 are sealed to avoid leakage; the liquid spraying end of the nozzle tip portion points to the position of the culture well plate. In addition, the nozzle tip portion of the present invention includes an integrally formed hollow cylindrical section tube body 2 and a hollow frustum section tip 1, the hollow cylindrical section tube body 2 is used to connect to the snap portion 3, and the hollow frustum section tip 1 is used to spray liquid and add samples into the culture well plate.

[0040] Compared with the prior art, the present invention can prevent leakage by connecting the pipette 8 and the connecting part of the nozzle unit 7 to form a closed structure; by setting the tip of the nozzle to a hollow cylindrical section tube body 2 and a hollow frustum section tip 1 structure, it can ensure that the organoids can pass through the automated dispenser pipeline smoothly and avoid organoids from clogging the pipeline.

[0041] It should be noted that the hollow frustum section tip 1 of the present invention includes a tip end and a frustum base end; the outer wall diameter of the frustum base end is larger than the outer wall diameter of the tip end; the outer wall diameter of the frustum base end is equal to the outer wall diameter of the hollow cylindrical section tube body 2.

[0042] It should be emphasized that the length of the hollow frustum section nozzle 1 of the present invention is 2.90mm; the inner wall diameter of the nozzle end of the hollow frustum section nozzle 1 is 0.50mm, and the outer wall diameter is 1mm; the outer wall diameter of the frustum base end of the hollow frustum section nozzle 1 is 2mm; the length of the hollow cylindrical section tube body 2 is 3.70mm, the inner wall diameter of the tube is 1.00mm, and the outer wall diameter of the tube is 2.00mm.

[0043] Specifically, the outer wall diameter of the frustum base end of the hollow frustum section tip 1 is 2 mm, and the outer wall diameter of the frustum base end of the hollow frustum section tip 1 is equal to the outer wall diameter of the hollow cylindrical section tube body 2, that is, the outer wall diameter of the hollow frustum section tip 1 gradually decreases from 2 mm at the frustum base end to 1 mm at the tip end.

[0044] Compared with the prior art, the present invention limits the sizes of the tip end and the base end of the hollow frustum segment tip 1, which can ensure that the organoids can pass through smoothly without blocking the pipeline.

[0045] The length of the hollow cylindrical section tube body 2 of the utility model is 3.70 mm, and the length of the hollow conical section tip 1 is 2.90 mm. Therefore, the overall length of the nozzle tip part (i.e., the hollow cylindrical section tube body 2 and the hollow conical section tip 1) is 6.60 mm. The length of the nozzle tip part is set to 6.60 mm. The size of the nozzle tip part is shortened compared to the existing nozzle. After the nozzle is shortened, the organoid can pass through the nozzle tip part more quickly under the condition that the liquid output remains unchanged, and the clogging of the organoid is reduced.

[0046] It should be pointed out that the material of the nozzle tip of the present invention is polypropylene, which is harder than medical silicone and can ensure the strength of the nozzle tip so that its positioning will not change due to the movement of the automated pipetting device during liquid separation. Polypropylene is also easier to clean, ensuring that the nozzle tip is sterile and clean.

[0047] The material of the pipette 8 of the present invention is medical silicone hose.

[0048] Specifically, the pipette 8 is made of a medical silicone hose, one end of which is placed in the organoid culture medium through an automated pipetting device for high-throughput separation and organoid plating; the other end is sealed and connected to the connecting part of the nozzle unit 7.

[0049] The pipeline parts of the automated liquid dispensing pipes in the prior art are mostly made of plastic products (for example, polypropylene); compared with the prior art, firstly, the pipette 8 of the utility model is a medical silicone hose, which is safer to use and more in line with clinical requirements; secondly, the medical silicone hose is easier to disinfect and can always be kept sterile and clean; furthermore, the medical silicone hose is highly flexible and can be bent flexibly, and will not cause pipeline blockage and liquid retention due to bending.

[0050] It should be noted that the medical silicone hose used in this utility model is transparent in appearance, with a smooth surface free of impurities, gel spots, and bubbles, meeting clinical requirements. The pipette 8 has a hardness of 60A (Shore). This is because: if the pipette 8 is too hard, liquid dispensing will not be possible. Since the automated pipetting device has a roller that rotates the pipette 8, if the material is too hard, the roller will not be able to squeeze the silicone hose, resulting in no liquid being dispensed. If the pipette 8 is too soft, it will bend, causing liquid blockage and stagnation in the pipette, resulting in no normal and even liquid dispensing.

[0051] To save costs, the pipette 8 of this invention has a tubing length of 500mm, an outer diameter of 3.30mm, an inner diameter of 1.30mm, a wall thickness of 2mm, and no fillers in the central cavity. The entire pipette 8 is manufactured in a cleanroom with a cleanroom rating of 100,000 or higher, achieving an initial bacterial count of less than 10 cfu / unit and an overall particle contamination index of less than 90.

[0052] Compared with the prior art, the present invention sets the length of the pipette 8 to 500 mm, which can meet the requirements of the automated pipetting device on the one hand, and on the other hand, reduce the loss of organoids, save costs, and avoid the loss of organoid samples due to the pipette 8 being too long.

[0053] The inner diameter of the pipette 8 of the present invention is 1.30 mm. This is because: a pipette 8 of this size can ensure the normal passage of the digested organoid without clogging. If the inner diameter of the pipette 8 is too small, it will cause clogging of the organoid, and if the inner diameter is too large, it will not be possible to accurately drip droplets.

[0054] In order to fix the nozzle tip part and the connecting part, the snap-fit ​​part 3 of the present invention includes a fixing block; a fixing groove is provided in the automated pipetting device, and the fixing block can be embedded in the fixing groove of the automated pipetting device; the snap-fit ​​part 3 is used to fix the nozzle tip part as a whole at a specified position of the automated pipetting device.

[0055] It should be pointed out that the fixing groove in the automated pipetting device is a rectangular groove, and the fixing block of the present invention is a rectangular fixing block, the size of which matches the size of the rectangular groove in the automated pipetting device.

[0056] The rectangular fixing block of the utility model has a length of 6.40 mm, a width of 4.50 mm, and a height of 1.10 mm, and is used to ensure that it can be inserted into the automated pipetting device, so that the tip of the nozzle can be firmly fixed in the specified position, ensuring that the tip of the nozzle will not move arbitrarily during the liquid dispensing process, causing inaccurate liquid dispensing.

[0057] In order to connect the nozzle unit 7 and the pipeline unit, the connecting part of the present invention includes a trapezoidal transition connecting pipe; the overall outer wall diameter of the trapezoidal transition connecting pipe is larger than the outer wall diameter of the pipette 8; the trapezoidal transition connecting pipe can be inserted into the pipette pipeline and form a closed structure with it.

[0058] Specifically, the connecting portion is used to connect the snap-fit ​​portion 3 of the nozzle unit 7 and the pipette 8 of the pipeline unit, and the trapezoidal transition connecting tube can be inserted into the pipette 8 to achieve a fixed connection between the two.

[0059] Compared with the prior art, the utility model sets the outer wall diameter of the trapezoidal transition connecting tube to be larger than the outer wall diameter of the pipette 8, which can ensure that after the connecting part is inserted into the pipette 8, a closed structure is formed between the nozzle tip part and the pipette 8; in addition, the utility model sets the connecting part as a trapezoidal transition connecting tube, which helps to insert the pipette 8 more smoothly.

[0060] On the premise of ensuring good sealing, in order to more smoothly insert the trapezoidal transition connecting tube into the pipette 8 as a whole, the trapezoidal transition connecting tube of the present invention includes a first cylindrical section 4, an intermediate frustum section 5 and a second cylindrical section 6 which are connected in sequence and are all hollow structures; wherein, the first cylindrical section 4 is fixedly connected to the snap part 3, the outer wall diameter of the first cylindrical section 4 is larger than the outer wall outer diameter of the second cylindrical section 6, and the outer wall diameters of the first cylindrical section 4, the intermediate frustum section 5 and the second cylindrical section 6 are all larger than the outer wall diameter of the pipette 8.

[0061] Specifically, although the outer wall diameters of the trapezoidal transition connecting tube (i.e., the first cylindrical section 4, the middle frustum section 5, and the second cylindrical section 6) are all larger than the inner wall diameter of the tube cavity of the pipette 8; however, since the pipette 8 is a medical silicone hose, it has relatively strong elasticity, so the second cylindrical section 6, the middle frustum section 5, and the first cylindrical section 4 can be inserted into the pipette 8 in sequence; in the process of inserting the second cylindrical section 6, the middle frustum section 5, and the first cylindrical section 4 into the pipette 8 in sequence, the middle frustum section 5 can play a good transition role, facilitating the smooth insertion of the first cylindrical section 4 into the pipette 8, so that the pipette 8 can completely wrap the entire trapezoidal transition connecting tube, and finally achieve a highly sealed connection between the nozzle tip and the pipette 8.

[0062] It should be pointed out that the middle frustum section 5 of the present invention includes a first circular bottom surface and a second circular bottom surface; wherein, the first circular bottom surface is adjacent to the first cylindrical section 4 and the outer wall diameter and inner wall diameter of the two are equal; the second circular bottom surface is adjacent to the second cylindrical section 6 and the outer wall diameter and inner wall diameter of the two are equal.

[0063] It should be noted that the size of the first cylindrical section 4 of the present invention is the same as the size of the hollow cylindrical section tube body 2 at the nozzle tip, that is, the length of the first cylindrical section 4 is 3.70 mm, the inner wall diameter is 1.00 mm, and the outer wall diameter is 2.00 mm.

[0064] Compared to the prior art, the present invention ensures the stability of the entire nozzle unit 7 by setting the dimensions of the first cylindrical section 4 and the hollow cylindrical section body 2 at the nozzle tip to the same parameters. If the first cylindrical section 4 is too small, it will cause organoids to clog; if it is too large, it will not be able to be smoothly inserted into the pipette 8. Similarly, if the hollow cylindrical section body 2 is too small, it will also cause organoids to clog; if it is too large, the nozzle tip will be too heavy, making the nozzle unit 7 unstable. When the dimensions of the first cylindrical section 4 and the hollow cylindrical section body 2 at the nozzle tip are the same, the center of gravity of the nozzle tip and the connecting part are centered, which is more conducive to the balance of the entire nozzle unit 7 and the fixation of the overall position.

[0065] It should also be noted that the length of the middle frustum section 5 of the present invention is 1.10 mm, the length of the second cylindrical section 6 is 3.70 mm; the outer wall diameter of the second cylindrical section 6 is 1.58 mm, and the inner wall diameter is 1.00 mm.

[0066] To further ensure a tight seal between the connecting portion and the pipette 8, the connecting portion is made of polypropylene. After the connecting portion's second cylindrical section 6, intermediate frustum section 5, and first cylindrical section 4 are sequentially inserted into the pipette 8, the pipette 8 is heated to bond the pipette 8 to the polypropylene connecting portion. Alternatively, an adhesive can be applied to the surface of the connection between the connecting portion and the pipette 8 to provide an even better seal.

[0067] It should be pointed out that the entire nozzle tip of the present invention is processed in a clean workshop of Class 100,000 or above, with an initial contaminating bacterial count of <10 cfu / piece, and an overall particle contamination index of no more than 90.

[0068] It should be emphasized that the material of the buckle part 3 and the connecting part of the present invention is the same as that of the nozzle tip part, both of which are made of medical-grade polypropylene (PP). The overall appearance is white, the surface is flat and free of impurities, gel spots and bubbles.

[0069] The overall length of the nozzle unit 7 is 16.2 mm. By controlling the overall length of the nozzle unit 7 to 16.2 mm, the overall length of the nozzle unit 7 is shortened compared with the existing technology. After the overall shortening of the nozzle unit 7, the clogging of the organoid is further reduced without affecting the discharge of the organoid and liquid.

[0070] It should be pointed out that the overall hardness of the nozzle tip of the utility model is 45D (Shore), which on the one hand ensures that the nozzle tip can be fixed more stably to ensure the accuracy of liquid separation; on the other hand, it ensures that the connecting part can be better inserted into the pipette 8 to form a closed environment.

[0071] In summary, the utility model can connect the nozzle tip part and the pipette 8 through the connecting part, so that a closed space is formed between the two; the entire nozzle unit 7 can be fixed to the specified position of the automated pipetting device through the snap-fit ​​part 3; the sample liquid can be smoothly sprayed out through the nozzle tip part to avoid clogging of the organoid.

[0072] Existing pipette tubing is typically made of plastic. Because it's used solely for separating liquids, it's rigid and has a narrow inner diameter. Consequently, when sorting organoid samples, large organoid clumps and viscous Matrigel often clog the tubing, causing machine stalls and inaccurate dispensing. This significantly hinders the high-throughput dispensing capabilities of automated dispensers.

[0073] Compared with the prior art, the nozzle tip portion of the present invention includes a hollow cylindrical section tube body 2 and a hollow conical section nozzle 1. The inner wall diameter of the hollow cylindrical section tube body 2 is 1.00 mm, and the outer wall diameter of the tube body is 2.00 mm; the inner wall diameter of the nozzle end of the hollow conical section nozzle 1 is 0.50 mm, and its outer wall diameter is 1 mm; the outer wall diameter of the conical base end of the hollow conical section nozzle 1 is 2 mm; by setting the nozzle tip portion into the above structure and limiting its size, the organoid can pass through the nozzle tip portion more quickly and the clogging of the organoid is reduced, that is, the present invention solves the problem of clogging of the pipette line.

[0074] Because the present invention solves the problem of pipette line blockage, it can use the entire automated dispenser for automated liquid separation, greatly improving liquid separation efficiency. Furthermore, the automated dispenser pipeline of the present invention can be used to separate multiple well plates and liquids of various volumes, further freeing up manual labor to achieve automation. In summary, the present invention can perform efficient automated organoid liquid separation, achieving high throughput and reducing labor costs.

[0075] Most existing pipette tubes are made of plastic. After using low-concentration disinfectant liquid to clean the existing plastic pipette tubes, they will cause them to oxidize and become hard and brittle. Therefore, they can only be cleaned with ordinary physiological solutions. After cleaning with ordinary physiological solutions, subsequent samples have an extremely high risk of contamination.

[0076] Compared to the prior art, the pipette 8 of the present invention uses a medical silicone hose, which is more corrosion-resistant and has a longer lifespan than plastic. The hose can be rinsed with a low-concentration disinfectant or alcohol. Simply place the cleaning solution into the dispenser inlet and run the cleaning procedure to completely clean the sample without contaminating it, making it safer for culturing biological samples such as organoids. In summary, the automated dispenser piping of the present invention is more convenient to clean.

[0077] The above is only a preferred specific implementation method of the present invention, but the protection scope of the present invention is not limited to this. Any changes or replacements that can be easily thought of by any technician familiar with this technical field within the technical scope disclosed in the present invention should be covered by the protection scope of the present invention.

Claims

1. An automated liquid dispenser pipeline, characterized in that: including a nozzle unit and a pipeline unit; The nozzle unit comprises a nozzle tip portion, a buckle portion and a connection portion connected in sequence; the nozzle tip portion comprises an integrally formed hollow cylindrical tube body and a hollow frustum-shaped tip; The hollow cylindrical tube body is used to connect with the buckle part, and the hollow frustum nozzle is used to spray liquid for sample addition; The pipeline unit includes a pipette, one end of which is connected to the organoid culture medium via an automated pipetting device, and the other end of which is fixedly connected to the connecting part.

2. The automated liquid dispenser pipeline according to claim 1, characterized in that: The hollow truncated cone segment tip includes a tip end and a truncated cone base end; The outer wall diameter of the frustum base end is greater than the outer wall diameter of the tip end; the outer wall diameter of the frustum base end of the hollow frustum section tip is equal to the outer wall diameter of the hollow cylindrical section tube body.

3. The automated dispenser pipeline according to claim 2, characterized in that: The length of the hollow truncated cone section tip is 2.90 mm; The inner wall diameter of the tip end of the hollow frustum section tip is 0.50 mm, the outer wall diameter is 1 mm, and the outer wall diameter of the frustum base end of the hollow frustum section tip is 2 mm.

4. The automated liquid dispenser pipeline according to claim 3, characterized in that: The length of the hollow cylindrical section tube body is 3.70 mm, the inner wall diameter is 1.00 mm, and the outer wall diameter is 2.00 mm.

5. The automated dispenser pipeline according to claim 1, characterized in that: The length of the pipette is 500 mm; the inner diameter of the lumen of the pipette is 1.30 mm; The pipette is made of medical silicone hose.

6. The automated liquid dispenser pipeline according to claim 1, characterized in that: The buckle part includes a fixing block; a fixing groove is provided in the automated pipetting device, and the fixing block can be embedded in the fixing groove of the automated pipetting device; the buckle part is used to fix the nozzle tip part as a whole at a specified position of the automated pipetting device.

7. The automated liquid dispenser pipeline according to claim 6, characterized in that: The fixing block is a rectangular parallelepiped fixing block, the fixing groove in the automated liquid transfer device is a rectangular parallelepiped groove, and the size of the rectangular parallelepiped fixing block matches the size of the rectangular parallelepiped groove in the automated liquid transfer device.

8. The automated liquid dispenser pipeline according to any one of claims 1 to 6, characterized in that: The connecting portion includes a trapezoidal transition connecting pipe; the outer wall diameter of the trapezoidal transition connecting pipe as a whole is larger than the outer wall diameter of the pipette; The trapezoidal transition connecting pipe can be inserted into the pipette and form a sealed structure therewith.

9. The automated liquid dispenser pipeline according to claim 8, characterized in that: The trapezoidal transition connecting pipe comprises a first cylindrical section, an intermediate truncated cone section and a second cylindrical section which are connected in sequence and are all hollow structures; The first cylindrical segment is fixedly connected to the buckle portion, the outer wall diameter of the first cylindrical segment is larger than the outer wall diameter of the second cylindrical segment, and the outer wall diameters of the first cylindrical segment, the middle frustum segment and the second cylindrical segment are all larger than the outer wall diameter of the pipette.

10. The automated dispenser pipeline according to claim 9, characterized in that: The middle frustum section includes a first circular bottom surface and a second circular bottom surface; wherein, the first circular bottom surface is adjacent to the first cylindrical section and the outer wall diameter and inner wall diameter of the two are equal; the second circular bottom surface is adjacent to the second cylindrical section and the outer wall diameter and inner wall diameter of the two are equal.

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