Separation culture device for pathogenic bacteria

Abstract

The utility model discloses a pathogenic bacteria separation culture device, and relates to the technical field of plant pathogenic bacteria. The device structurally comprises an outer box body, an inner pressure seat and a separator, the outer box body is an annular cup body with an upper opening; the lower half part of the outer box body is a conical cylinder part; the upper half part of the outer box body is a cylinder part; a liquid outlet is formed in the outer wall of the cylinder part; a flow guide nozzle matched with the liquid outlet is arranged on the outer wall of the cylinder part; the inner pressing seat comprises a cylinder part at the upper half part and a cylinder frustum part at the lower half part; a circular through hole is formed in the center of the inner pressure seat; the outer wall of the cylinder part is in sliding fit with the inner wall of the cylinder part; and the separator penetrates through the circular through hole. According to the utility model, the outer box body, the inner pressure seat and the separator are integrated, so that the pathogenic bacteria separation culture process is more coherent and efficient. A plurality of scattered appliances are not needed, so that the operation steps and time are reduced, and the working efficiency is improved; the cleaning, disinfection and separation processes of green and dead leaves can be completed through simple actions of placing, shaking, dumping and the like.

Description

Technical Field

[0001] The utility model belongs to the technical field of plant pathogens, in particular to a pathogen separation and cultivation device. Background Art

[0002] In plant pathology research and practice, the isolation and culture of pathogens is a crucial step in the diagnosis and prevention of plant diseases. Traditional methods for pathogen isolation and culture often have limitations and shortcomings, specifically the following: First, traditional methods require the use of multiple separate instruments, such as sampling tools, sterilization containers, and separation tools. These instruments not only increase operational complexity and time costs but are also prone to confusion and contamination during operation, compromising the accuracy and reliability of isolation and culture. For example, cleaning and sterilizing leaf samples requires constant replacement of containers and liquids, making the process cumbersome and error-prone. Second, traditional methods lack precision and efficiency in sample handling and separation. After cleaning and sterilizing leaf samples, manual cutting or crushing is often required for separation, which not only easily leads to sample inhomogeneity and contamination, but also makes it difficult to ensure consistent separation results. Furthermore, separated samples are prone to loss or mix-up during transfer and culture, complicating subsequent analysis and management.

[0003] Therefore, there is an urgent need for an integrated, efficient, precise, safe and reliable pathogen separation and culture device to improve the efficiency and quality of separation and culture and meet the needs of plant pathology research and practice. Utility Model Content

[0004] The purpose of the utility model is to provide a pathogen separation and culture device, which integrates an outer box body, an inner pressure seat and a separator into one body, so that the pathogen separation and culture process is more consistent and efficient.

[0005] In order to solve the above technical problems, the present invention is achieved through the following technical solutions:

[0006] The utility model is a pathogen separation and culture device, comprising an outer box body, an inner pressure seat and a separator; the outer box body is an annular cup body with an upper opening; the lower half of the outer box body is a conical cylinder; the upper half of the outer box body is a cylindrical portion; the outer wall of the cylindrical portion is provided with a liquid outlet; the outer wall of the cylindrical portion is provided with a guide nozzle that cooperates with the liquid outlet; the inner pressure seat comprises a cylindrical body portion in the upper half and a cylindrical frustum portion in the lower half; a circular through hole is provided in the center of the inner pressure seat; the outer wall of the cylindrical body portion is slidably matched with the inner wall of the cylindrical portion; and the separator passes through the circular through hole.

[0007] As a preferred technical solution of the present invention, the separator includes a cylindrical body; a cylindrical separation pressure head arranged in a rectangular array is provided at the lower end of the cylindrical body, and a spherical notch is provided on the lower end surface of the cylindrical separation pressure head.

[0008] As a preferred technical solution of the present invention, the inner bottom of the conical cylinder portion is provided with an upwardly protruding frustum portion.

[0009] As a preferred technical solution of the present invention, a circle of evenly distributed cylindrical rods is provided on the top surface of the truncated cone portion near its edge, and the tops of the cylindrical rods are configured as hemispherical surfaces.

[0010] As a preferred technical solution of the present invention, two anti-slip grooves are axially symmetrically provided on the outer wall of the upper end portion of the cylindrical body.

[0011] As a preferred technical solution of the present invention, a sealing cover is also included.

[0012] The utility model has the following beneficial effects:

[0013] 1. The utility model integrates the outer box, inner pressure seat and separator into one body, making the pathogen isolation and cultivation process more consistent and efficient. It does not require multiple separate instruments, reduces the operation steps and time, and improves work efficiency.

[0014] 2. The design of this utility model makes the operation easier and more convenient. The cleaning, disinfection and separation of green and dead leaves can be completed by simple actions such as placing, shaking and pouring.

[0015] 3. This device uses multiple cleaning and disinfection methods to ensure that impurities and pathogens on the surface of dead leaves are fully removed. First, they are soaked in 75% ethanol, which has a good bactericidal effect; then soaked in 5% sodium hypochlorite solution to further enhance the disinfection effect; finally, they are rinsed multiple times with sterile water to ensure that no disinfectant residue is left.

[0016] 4. The cylindrical separation head of this utility model can separate the dead leaves into nine sterilized discs, achieving precise separation of samples. Each disc can be cultured and observed separately, facilitating subsequent analysis and management.

[0017] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0019] Figure 1 This is a structural diagram of the utility model when the outer box body and the inner pressure seat are combined;

[0020] Figure 2 for Figure 1 Schematic diagram of the axial section;

[0021] Figure 3 It is a structural diagram of the outer box body, the inner pressure seat and the separator when they are combined;

[0022] Figure 4 It is a structural diagram of the outer box body, the inner pressure seat and the cover when they are combined;

[0023] Figure 5 Schematic diagram of the structure of the outer box;

[0024] Figure 6 Schematic diagram of the structure of the inner pressure seat;

[0025] Figure 7 It is a structural diagram of the separator;

[0026] Figure 8 Schematic diagram of the axial section of the separator.

[0027] In the accompanying drawings, the components represented by the reference numerals are as follows:

[0028] 1-outer box body, 2-inner pressure seat, 3-separator, 4-cover, 11-conical cylinder, 12-cylindrical part, 13-liquid outlet, 14-flow nozzle, 15-conical part, 16-cylindrical rod, 21-cylindrical body, 22-cylindrical frustum, 23-circular through hole, 31-cylindrical main body, 32-cylindrical separation pressure head, 33-spherical notch, 34-anti-slip groove. DETAILED DESCRIPTION

[0029] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention. Specific embodiment one:

[0031] See also Figure 1-8 As shown, the utility model is a pathogen separation and culture device, comprising an outer box body 1, an inner pressure seat 2 and a separator 3. The outer box body 1 is an annular cup body with an upper opening. The lower half of the outer box body 1 is a conical cylinder 11. The upper half of the outer box body 1 is a cylindrical portion 12. A liquid outlet 13 is provided on the outer wall of the cylindrical portion 12. A flow guide nozzle 14 that cooperates with the liquid outlet 13 is provided on the outer wall of the cylindrical portion 12. The inner pressure seat 2 includes a cylindrical body 21 in the upper half and a cylindrical frustum 22 in the lower half. A circular through hole 23 is provided in the center of the inner pressure seat 2. The outer wall of the cylindrical body 21 is slidably matched with the inner wall of the cylindrical portion 12. The separator 3 passes through the circular through hole 23.

[0032] To facilitate the separation of dead leaves from the debris detached from them, an upwardly protruding conical portion 15 is provided on the inner bottom of the conical portion 11 to increase the depth of the debris deposition. Dead leaves are placed on the top surface of the conical portion 15. During the cleaning and shaking process, the debris slides off the conical portion 15 and enters the annular bottom. During the pouring process, the debris moves from the periphery of the conical portion 15 to the lower side, and finally flows out of the diversion nozzle 14 for pouring.

[0033] In order to confine the dead leaves within the range of the truncated cone portion 15, a circle of evenly distributed cylindrical rods 16 is provided on the top surface of the truncated cone portion 15 near its edge, and the top of the cylindrical rods 16 is set to a hemispherical surface.

[0034] The separator 3 includes a cylindrical body 31. The lower end of the cylindrical body 31 is provided with cylindrical separation pressure heads 32 arranged in a rectangular array, and the lower end surface of the cylindrical separation pressure heads 32 is provided with a spherical notch 33, and the diameter of the spherical notch 33 is 2-3 mm.

[0035] In order to facilitate the taking and placing of the separator 3 , two anti-slip grooves 34 are axially symmetrically provided on the outer wall of the upper end of the cylindrical body 31 .

[0036] In order to prevent liquid from splashing out when the outer box body 1 is shaken, a cover 4 is provided. The cover 4 is installed at the end of the round through hole 23 when shaking.

[0037] When carrying out pathogen isolation and culture, first place the withered leaf on the truncated cone portion 15, then place the inner pressure seat 2, and under the action of gravity, the bottom surface of the inner pressure seat 2 falls on the top of a circle of evenly distributed cylindrical rods 16. Pour 75% ethanol from the round through hole 23 of the inner pressure seat 2 and soak for more than 2 minutes, pick up the outer box body 1 and shake it back and forth to clean and disinfect the surface of the withered leaf, and then tilt the outer box body 1 to pour out the ethanol. Then pour 5% sodium hypochlorite solution from the round through hole 23 of the inner pressure seat 2 and soak for more than 1 minute, pick up the outer box body 1 and shake it back and forth to clean and disinfect the surface of the withered leaf, and then tilt the outer box body 1 to pour out the sodium hypochlorite solution. Then pour sterile water from the round through hole 23 of the inner pressure seat 2, pick up the outer box body 1 and shake it back and forth to clean the surface of the withered leaf, and then tilt the outer box body 1 to pour out the sterile water. Subsequent use of sterile water twice is carried out for cleaning, which will not be repeated here. The sterilized separator 3 is inserted through the circular through-hole 23 of the internal pressure seat 2. The cylindrical separation head 32 at the lower end of the separator 3 separates the nine sterilized leaf discs. After removing the separator 3 and the internal pressure seat 2, the leaf discs are picked up with tweezers, dehydrated with sterile absorbent paper, and then placed in a culture dish filled with PDA culture medium for incubation.

[0038] Throughout this specification, references to terms such as "one embodiment," "example," or "specific example" indicate that a specific feature, structure, material, or characteristic described in conjunction with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0039] The preferred embodiments of the present invention disclosed above are intended only to help illustrate the present invention. The preferred embodiments do not describe all details in detail, nor do they limit the present invention to the specific embodiments described. Obviously, many modifications and variations are possible based on the contents of this specification. These embodiments are selected and described in detail in this specification to better explain the principles and practical applications of the present invention, thereby enabling those skilled in the art to better understand and utilize the present invention. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A pathogen separation and culture device, characterized in that: It comprises an outer box body (1), an inner pressure seat (2) and a separator (3); The outer box body (1) is an annular cup body with an upper opening; the lower half of the outer box body (1) is a conical cylinder (11); the upper half of the outer box body (1) is a cylindrical cylinder (12); the outer wall of the cylindrical cylinder (12) is provided with a liquid outlet (13); the outer wall of the cylindrical cylinder (12) is provided with a guide nozzle (14) that cooperates with the liquid outlet (13); The inner pressure seat (2) comprises an upper cylindrical portion (21) and a lower cylindrical frustum portion (22); a circular through hole (23) is provided at the center of the inner pressure seat (2); the outer wall of the cylindrical portion (21) is in sliding engagement with the inner wall of the cylindrical portion (12); The separator (3) passes through the circular through hole (23).

2. The pathogen separation and culture device according to claim 1, characterized in that: The separator (3) comprises a cylindrical main body (31); cylindrical separation pressure heads (32) arranged in a rectangular array are provided at the lower end of the cylindrical main body (31), and a spherical notch (33) is provided on the lower end surface of the cylindrical separation pressure heads (32).

3. The pathogen separation and culture device according to claim 1, characterized in that: The inner bottom of the conical cylinder portion (11) is provided with an upwardly protruding truncated cone portion (15).

4. The pathogen separation and culture device according to claim 3, characterized in that: A circle of evenly distributed cylindrical rods (16) is provided on the top surface of the truncated cone portion (15) near its edge, and the top of the cylindrical rods (16) is configured as a hemispherical surface.

5. The pathogen separation and culture device according to claim 2, characterized in that: Two anti-slip grooves (34) are axially symmetrically formed on the outer wall of the upper end portion of the cylindrical body (31).

6. The pathogen separation and culture device according to any one of claims 1 to 5, characterized in that: Also included is a cover (4).

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