Protein purification instrument

Abstract

The utility model discloses a protein purification instrument which comprises a shell, the inner bottom wall of the shell is movably connected with a base, the middle part of the upper surface of the base is fixedly connected with a fixed seat, and the edge of the upper surface of the fixed seat is fixedly connected with a fixed plate through a bolt. According to the protein purification instrument, through the arrangement of a sleeve, a connecting rod, a cam handle, a push plate and a rubber column, the position of the connecting rod in the sleeve is changed, and the strokes of a first connecting plate and a second connecting plate are adjusted, so that the clamping force is changed, and the protein purification instrument is suitable for clamping test tubes with different sizes within a certain range; two groups of push plates are used for clamping test tubes through rubber columns, so that a plurality of test tubes can be limited at the same time, the workload is reduced, the efficiency is greatly improved, rigid contact during fixing is reduced by the rubber columns, and meanwhile, the limiting effect is enhanced; and the use safety and stability are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of purifiers, in particular to a protein purifier. Background Art

[0002] Protein is an important component of all cells and tissues in the human body. All important components of the body require the participation of protein. The separation and purification of protein is widely used in biochemical research applications and is an important operating technology. In order to study a certain protein, the protein must first be purified from other proteins and non-protein molecules. Protein purification requires a protein purifier.

[0003] After searching, Chinese patent publication (announcement) No. CN218465724U discloses a multi-channel protein purifier, including a housing, a mounting block and a base, the top of the base is equipped with a housing, the second limiting plate and the two ends of the first limiting plate are equipped with side plates, and the two sides of the threaded rod are equipped with mounting plates. The utility model is achieved by installing a first limiting plate on the top of the base, the first limiting plate and the second limiting plate cooperate with each other, the threaded rod is movably connected to the inner side of the mounting plate, the threaded rod and the side plates at both ends of the first limiting plate and the second limiting plate are threadedly connected, and the threaded rod is provided with threads symmetrically distributed about the central axis of the threaded rod. Twisting the threaded rod can drive the first limiting plate and the second limiting plate to move in opposite directions. The movement of the first limiting plate and the second limiting plate can clamp the test tube in the middle, limit the test tube, and prevent the test tube from deviating during processing, thereby achieving the purpose of facilitating the positioning of the test tube in the multi-channel protein purifier.

[0004] Although the protein purifier of the above patent can limit the test tubes, the method of limiting the test tubes by using threaded rods and limiting plates is relatively complicated to operate. When there are many test tubes, the staff needs to limit multiple test tubes one by one, and after the purification is completed, they need to release the limits one by one, which is time-consuming and labor-intensive, and has certain limitations in use. Therefore, it is necessary to design a protein purifier to solve the above problems. Utility Model Content

[0005] The main purpose of the present invention is to provide a protein purification instrument that can effectively solve the problems in the background technology.

[0006] In order to achieve the above purpose, the technical solution adopted by the utility model is:

[0007] A protein purifier comprises a shell, wherein the inner bottom wall of the shell is movably connected to a base, the middle part of the upper surface of the base is fixedly connected to a fixing seat, the edge of the upper surface of the fixing seat is fixedly connected to a fixing plate by bolts, one side of the lower surface of the fixing plate is rotatably connected to a first connecting plate; the other side of the lower surface of the fixing plate is rotatably connected to a second connecting plate, one end of the first connecting plate is rotatably connected to a sleeve, the internal thread of the sleeve is connected to a connecting rod, one end of the connecting rod is rotatably connected to a cam handle, the middle parts of the lower surfaces of the first and second connecting plates are fixedly connected to push plates, and the outer surfaces of the push plates are fixedly connected to rubber columns.

[0008] In order to facilitate the resetting of the push plate, as a protein purifier of the present invention, first grooves are provided on both sides of the outer surface of the fixing seat, and a first spring is fixedly connected to the interior of the first groove.

[0009] In order to achieve the effect of avoiding hard contact of the test tube, as a protein purification instrument of the present invention, both sides of the inner side surface of the fixing seat are fixedly connected with rubber seats.

[0010] In order to facilitate the fixing of the base, a protein purifier of the present invention has second grooves on both sides of the outer surface of the base, a second spring is fixedly connected to the inside of the second groove, one end of the second spring is fixedly connected to a limiting column, and a limiting hole is provided on the outer surface of the shell close to the limiting column.

[0011] In order to facilitate the adjustment of the position of the support plate, a protein purifier of the present invention is provided with a cylinder fixedly connected to the middle of the upper surface of the shell, the output end of the cylinder is fixedly connected to the support plate, and the four corners of the upper surface of the support plate are fixedly connected to guide rods, and the guide rods are slidably connected to the shell.

[0012] In order to achieve the purification effect, as a protein purifier of the present invention, the lower surface of the support plate is fixedly connected to the purifier, and the top of the outer surface of the purifier is fixedly connected to the rubber pad.

[0013] In order to facilitate the opening and closing of the magnetic door, a protein purifier of the present invention has connectors fixedly connected to the corners of the upper and lower surfaces of the shell, and the inner side of the connector is rotatably connected to the magnetic door.

[0014] In order to achieve the effect of enhancing the sealing of the magnetic door, as a protein purifier of the present invention, a sealing gasket is fixedly connected to the edge of the back of the magnetic door, and a transparent window is fixedly connected to the middle of the front of the magnetic door.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] 1. In the utility model, the position of the connecting rod inside the sleeve is changed by arranging the sleeve, the connecting rod, the cam handle, the push plate and the rubber column, and the stroke of the first connecting plate and the second connecting plate is adjusted, thereby changing the clamping force. It is suitable for clamping test tubes of different sizes within a certain range. The cam handle is rotated horizontally to make the connecting rod and the sleeve drive the second connecting plate and the first connecting plate to move closer to each other. The two sets of push plates clamp the test tubes through the rubber columns, and can limit multiple test tubes at the same time, reducing workload and greatly improving efficiency. The rubber columns reduce hard contact during fixation, and at the same time the limiting effect is enhanced, thereby increasing safety and stability in use.

[0017] 2. In the utility model, through the arrangement of the first spring, the rubber seat, the limiting column and the limiting hole, after the cam handle is rotated to release the limit, the first connecting plate and the second connecting plate can be reset more conveniently under the elastic action of the first spring. The rubber seat facilitates the placement of the test tube, avoids hard contact between the test tube and the fixed seat, and the protective performance is enhanced. The limiting column is pressed to shrink to the inside of the second groove, and then the base is placed in the inner position of the shell. Under the elastic action of the second spring, the limiting column is reset and clamped in the inside of the limiting hole, thereby fixing the base, avoiding the base from moving back and forth during use, making the installation and disassembly of the base more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the main structure of an embodiment of the utility model;

[0019] Figure 2 This is a schematic diagram of the internal structure of the housing according to an embodiment of the present utility model;

[0020] Figure 3 This is a schematic diagram of the base structure of an embodiment of the utility model;

[0021] Figure 4 This is a schematic diagram of the exploded structure of the base of an embodiment of the utility model;

[0022] Figure 5 This is a schematic diagram of the support plate structure of an embodiment of the present utility model.

[0023] In the figure: 1. Shell; 2. Base; 3. Fixed seat; 4. Fixed plate; 5. First connecting plate; 6. Second connecting plate; 7. Sleeve; 8. Connecting rod; 9. Cam handle; 10. Push plate; 11. Rubber column; 12. First groove; 13. First spring; 14. Rubber seat; 15. Second groove; 16. Second spring; 17. Limiting column; 18. Limiting hole; 19. Cylinder; 20. Support plate; 21. Guide rod; 22. Purifier; 23. Rubber pad; 24. Connector; 25. Magnetic door; 26. Sealing pad; 27. Transparent window. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention. Example

[0025] like Figure 1-5 As shown, a protein purification instrument includes a shell 1, the inner bottom wall of the shell 1 is movably connected to the base 2, the middle part of the upper surface of the base 2 is fixedly connected to the fixing seat 3, the edge of the upper surface of the fixing seat 3 is fixedly connected to the fixing plate 4 by bolts, one side of the lower surface of the fixing plate 4 is rotatably connected to the first connecting plate 5; the other side of the lower surface of the fixing plate 4 is rotatably connected to the second connecting plate 6, one end of the first connecting plate 5 is rotatably connected to the sleeve 7, the internal thread of the sleeve 7 is connected to the connecting rod 8, one end of the connecting rod 8 is rotatably connected to the cam handle 9, the middle part of the lower surface of the first connecting plate 5 and the second connecting plate 6 is fixedly connected to the push plate 10, and the outer surface of the push plate 10 is fixedly connected to the rubber column 11.

[0026] When in use, the fixing seat 3 is fixed to the middle of the upper surface of the base 2 through the arrangement of the fixing plate 4, the first connecting plate 5, the second connecting plate 6, the sleeve 7, the connecting rod 8, the cam handle 9, the push plate 10 and the rubber column 11. The fixing seat 3 is fixed to the middle of the upper surface of the base 2, which is an H-shaped structure. The fixing plate 4 is fixed to one side of the upper surface of the fixing seat 3 by bolts. It is easy to install and disassemble, and is convenient for replacement and maintenance when damaged. There is no need to replace the overall structure, which saves costs. The first connecting plate 5 and the second connecting plate 6 are rotatably connected to the two sides of the lower surface of the fixing plate 4 respectively, the sleeve 7 is rotatably connected to the first connecting plate 5, and the connecting rod 8 is threadedly connected to the inside of the sleeve 7. By changing the position of the connecting rod 8 inside the sleeve 7, the stroke of the first connecting plate 5 and the second connecting plate 6 can be adjusted, thereby changing the clamping force. It is used for clamping test tubes of different sizes within a certain range. At the same time, the connecting rod 8 is slidably connected to the inside of the second connecting plate 6 and is connected to the cam handle 9. Two groups of push plates 10 are respectively fixed to the middle of the lower surface of the first connecting plate 5 and the second connecting plate 6. Multiple test tubes are placed on both sides of the inner side of the fixing seat 3. The cam handle 9 is rotated horizontally to make the connecting rod 8 and the sleeve 7 drive the second connecting plate 6 and the first connecting plate 5 to move closer to each other. The two groups of push plates 10 clamp the test tubes through the rubber column 11, and can limit multiple test tubes at the same time, reducing workload and greatly improving efficiency. The rubber column 11 has flexibility and high friction, which reduces hard contact during fixation. At the same time, the limiting effect is enhanced, increasing safety and stability in use.

[0027] In this embodiment, first grooves 12 are formed on both sides of the outer surface of the fixing seat 3 , and a first spring 13 is fixedly connected inside the first groove 12 .

[0028] During specific use, through the setting of the first spring 13, the two groups of first springs 13 are respectively fixed on both sides of the inside of the fixing seat 3 through the first groove 12. After the cam handle 9 is rotated to release the limit, the elastic action of the first spring 13 can make the first connecting plate 5 and the second connecting plate 6 more convenient to reset.

[0029] In this embodiment, rubber seats 14 are fixedly connected to both sides of the inner side surface of the fixing seat 3 .

[0030] During specific use, the rubber seat 14 is provided and fixed to the inner side of the base 2 , which facilitates the placement of the test tube and avoids hard contact between the test tube and the fixing seat 3 , thereby enhancing the protective performance.

[0031] In this embodiment, a second groove 15 is provided on both sides of the outer surface of the base 2, a second spring 16 is fixedly connected to the inside of the second groove 15, one end of the second spring 16 is fixedly connected to a limiting column 17, and a limiting hole 18 is provided on the outer surface of the shell 1 near the limiting column 17.

[0032] During specific use, through the setting of the limiting column 17 and the limiting hole 18, the limiting column 17 is pressed to shrink it to the inside of the second groove 15, and then the base 2 is placed in the inner position of the shell 1. Under the elastic action of the second spring 16, the limiting column 17 is reset and clamped in the inside of the limiting hole 18, thereby fixing the base 2 to prevent the base 2 from moving back and forth during use, making the installation and disassembly of the base 2 more convenient.

[0033] In this embodiment, a cylinder 19 is fixedly connected to the middle of the upper surface of the shell 1, a support plate 20 is fixedly connected to the output end of the cylinder 19, and guide rods 21 are fixedly connected to the four corners of the upper surface of the support plate 20, and the guide rods 21 are slidably connected to the shell 1.

[0034] During specific use, through the setting of the cylinder 19, the output end of the cylinder 19 is extended and retracted, and the position of the support plate 20 can be adjusted, which is suitable for test tubes of different heights within a certain range. The support plate 20 supports the purifier 22, and four groups of guide rods 21 are fixed at the four corners of the upper surface of the support plate 20. They change position as the support plate 20 moves, preventing the support plate 20 from shifting in position and angle during movement, thereby improving the accuracy of displacement.

[0035] In this embodiment, a purifier 22 is fixedly connected to the lower surface of the support plate 20 , and a rubber pad 23 is fixedly connected to the top of the outer surface of the purifier 22 .

[0036] During specific use, through the setting of the rubber pad 23, multiple purifiers 22 move downward under the action of the support plate 20, thereby purifying the protein inside the test tube. After the support plate 20 moves, the rubber pad 23 contacts the top of the test tube, limits and fixes the top of the test tube, and at the same time provides a buffer space between the support plate 20 and the test tube.

[0037] In this embodiment, connectors 24 are fixedly connected to the corners of the upper and lower surfaces of the housing 1 , and a magnetic door 25 is rotatably connected to the inner side of the connector 24 .

[0038] During specific use, through the setting of the magnetic door 25, the two sets of connecting parts 24 support the magnetic door 25, making it more convenient to rotate and open and close the magnetic door 25. When the magnetic door 25 is closed, it can be attracted to the shell 1 to ensure stability during use.

[0039] In this embodiment, a sealing gasket 26 is fixedly connected to the edge of the back side of the magnetic door 25 , and a transparent window 27 is fixedly connected to the middle of the front side of the magnetic door 25 .

[0040] During specific use, through the setting of the sealing gasket 26, when the magnetic door 25 is closed, the sealing gasket 26 is clamped inside the shell 1, thereby strengthening the sealing of the connection between the magnetic door 25 and the inner side of the shell 1, and the sealing effect is enhanced. Through the transparent window 27, it is convenient for the staff to check the status of protein purification inside the shell 1 from the outside.

[0041] Working principle: During use, press the limiting column 17 to place the base 2 in the inner position of the shell 1. Under the elastic action of the second spring 16, the limiting column 17 is reset and clamped in the inner position of the limiting hole 18, fixing the base 2, changing the position of the connecting rod 8 in the sleeve 7, adjusting the stroke of the first connecting plate 5 and the second connecting plate 6, changing the clamping force according to the size of the test tube, placing multiple test tubes to be purified in the inner position of the rubber seat 14, and rotating the cam handle 9 in the horizontal direction so that the connecting rod 8 and the sleeve 7 drive the second connecting plate 6 and the first connecting plate 5 to move relative to each other. The two push plates 10 move closer to each other, and the two groups of push plates 10 clamp the test tubes through the rubber columns 11, which can limit multiple test tubes at the same time. The magnetic door 25 is closed, and the output end of the cylinder 19 is extended, so that the support plate 20 drives the purifier 22 to contact the test tube to purify the protein inside the test tube. The rubber pad 23 limits and fixes the top of the test tube. After purification is completed, the magnetic door 25 is opened, and the cam handle 9 is rotated in the opposite direction. Under the elastic action of the two groups of first springs 13, the first connecting plate 5 and the second connecting plate 6 are reset, and the purified protein test tube is taken out for the next operation.

[0042] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and improvements may be made to the present invention without departing from the spirit and scope of the present invention. Such changes and improvements are intended to fall within the scope of the present invention. The scope of protection claimed in this invention is defined by the appended claims and their equivalents.

Claims

1. A protein purification apparatus, comprising a housing (1), characterized in that: The inner bottom wall of the shell (1) is movably connected to a base (2), the middle of the upper surface of the base (2) is fixedly connected to a fixing seat (3), the edge of the upper surface of the fixing seat (3) is fixedly connected to a fixing plate (4) by bolts, and one side of the lower surface of the fixing plate (4) is rotatably connected to a first connecting plate (5); The other side of the lower surface of the fixed plate (4) is rotatably connected to a second connecting plate (6), one end of the first connecting plate (5) is rotatably connected to a sleeve (7), the internal thread of the sleeve (7) is connected to a connecting rod (8), one end of the connecting rod (8) is rotatably connected to a cam handle (9), the middle parts of the lower surfaces of the first connecting plate (5) and the second connecting plate (6) are fixedly connected to a push plate (10), and the outer surface of the push plate (10) is fixedly connected to a rubber column (11).

2. A protein purification instrument according to claim 1, characterized in that: First grooves (12) are provided on both sides of the outer surface of the fixing seat (3), and a first spring (13) is fixedly connected inside the first groove (12).

3. A protein purification instrument according to claim 1, characterized in that: Both sides of the inner side surface of the fixing seat (3) are fixedly connected with rubber seats (14).

4. A protein purification instrument according to claim 1, characterized in that: A second groove (15) is provided on both sides of the outer surface of the base (2), a second spring (16) is fixedly connected inside the second groove (15), one end of the second spring (16) is fixedly connected to a limiting column (17), and a limiting hole (18) is provided on the outer surface of the housing (1) near the limiting column (17).

5. A protein purification instrument according to claim 1, characterized in that: A cylinder (19) is fixedly connected to the middle of the upper surface of the shell (1), an output end of the cylinder (19) is fixedly connected to a support plate (20), and guide rods (21) are fixedly connected to the four corners of the upper surface of the support plate (20), and the guide rods (21) are slidably connected to the shell (1).

6. A protein purification instrument according to claim 5, characterized in that: A purifier (22) is fixedly connected to the lower surface of the support plate (20), and a rubber pad (23) is fixedly connected to the top of the outer surface of the purifier (22).

7. A protein purification instrument according to claim 1, characterized in that: Connecting pieces (24) are fixedly connected to the corners of the upper and lower surfaces of the shell (1), and the inner side surface of the connecting piece (24) is rotatably connected to a magnetic door (25).

8. A protein purification instrument according to claim 7, characterized in that: A sealing gasket (26) is fixedly connected to the edge of the back side of the magnetic door (25), and a transparent window (27) is fixedly connected to the middle of the front side of the magnetic door (25).

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