Multifunctional cell incubator

Abstract

The utility model discloses a multifunctional cell incubator which comprises an incubator main body, placing plates, an air inlet pipe, an air blowing pipe and an air outlet pipe, a plurality of horizontally arranged placing plates are arranged in the incubator main body, the placing plates are sequentially arranged from top to bottom, an air outlet area is formed between the lowermost placing plate and the bottom wall of the incubator main body, and the air outlet area is communicated with the air blowing pipe. A plurality of storage areas are formed between the adjacent storage plates and between the storage plate at the topmost end and the top wall of the incubator body, an ultraviolet sterilization lamp and a horizontally-arranged air blowing pipe are arranged in each storage area, a plurality of air blowing holes are formed in each air blowing pipe, the air inlet pipe is inserted into the incubator body from the upper portion of the incubator body, and the air outlet pipe is inserted into the incubator body from the lower portion of the incubator body. The air inlet pipe is communicated with all the air blowing pipes; according to the cell culture box, the first connecting pipe and the second branch pipe are connected with the culture box main body, and the fan and the second connecting pipe are matched, so that gas in the culture box can be quickly and fully mixed, and the use efficiency of the cell culture box is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of incubators, in particular to the technical field of cell incubators. Background Art

[0002] Traditional cell culture incubators use ventilation systems to control the gas composition inside the incubator, usually by injecting pure carbon dioxide gas into the interior and mixing it with the internal air to achieve the required carbon dioxide concentration. This process requires the use of computing devices such as chips to ensure the accuracy of the gas composition.

[0003] However, in traditional incubators, gas mixing takes time, usually 8 hours or even longer for the carbon dioxide concentration in each layer inside the entire incubator to reach the required level. Such a delay may affect the growth rate and quality of cells, thereby reducing production efficiency and product quality. In traditional cell culture incubators, manual intervention is indispensable. The cell factory needs to be manually placed in the incubator and taken out when needed. This manual operation may not only affect the stability of the culture environment, but is also not suitable for large-scale production automation needs. Summary of the Invention

[0004] The purpose of the utility model is to solve the problems in the prior art and to provide a multifunctional cell culture box, which can quickly and evenly mix the other components in the cell culture box and can perform sterilization and disinfection operations.

[0005] To achieve the above-mentioned purpose, the utility model proposes a multifunctional cell culture box, including an incubator body, a storage plate, an air inlet pipe, an air blow pipe, and an air outlet pipe. The incubator body is provided with a plurality of horizontally arranged storage plates, which are arranged in sequence from top to bottom. An air outlet area is formed between the lowest storage plate and the bottom wall of the incubator body, and a plurality of storage areas are formed between adjacent storage plates and between the topmost storage plate and the top wall of the incubator body. Each storage area is provided with an ultraviolet sterilization lamp and a horizontally arranged air blow pipe, and the air blow pipe is provided with a plurality of air blow holes. The air inlet pipe is inserted into the interior of the incubator body from the top of the incubator body, and the air inlet pipe is connected to all the air blow pipes. The air inlet pipe passes through all the storage plates except the lowest storage plate. All the storage plates, the air inlet pipe is connected to a plurality of first branch pipes, each first branch pipe is connected to an air supply device, the incubator body is connected to a first connecting pipe, the first connecting pipe is connected to the air outlet area, the first connecting pipe is connected to the air inlet end of a fan located outside the incubator body, the air outlet end of the fan is connected to a second connecting pipe, the second connecting pipe is connected to a plurality of second branch pipes, the second branch pipes correspond to the storage areas one by one, and the second branch pipes are connected to the corresponding storage areas, the incubator body is connected to an air outlet pipe, the air outlet pipe is connected to the air outlet area, the air outlet pipe is connected to the air intake end of a gas processing device located outside the incubator body and having an active air suction function, and the first branch pipe, the air inlet pipe, and the air outlet pipe are all connected to an electric valve.

[0006] Preferably, one side of the incubator body is a door, the storage plate is fixed to the three inner walls of the incubator body, and there is a gap between the storage plate and the door.

[0007] Preferably, the air blowing pipe is fixed on the inner wall of the incubator body away from the door, and the air inlet end of the first connecting pipe and the air outlet end of the second branch pipe are both located on the side of the incubator body away from the door.

[0008] Preferably, the air inlet pipe consists of a straight pipe and an annular pipe, the annular pipe is connected to a straight pipe, the straight pipe is inserted into the incubator body, the first branch pipe is fixed on the annular pipe, and the first branch pipe is communicated with the annular pipe.

[0009] The beneficial effects of the present invention are as follows: the present invention connects the first connecting pipe and the second branch pipe to the incubator body, and cooperates with the fan and the second connecting pipe, so that the gas in the incubator can be quickly and fully mixed, thereby improving the utilization efficiency of the cell culture incubator; the setting of the ultraviolet sterilization lamp enables the cell culture incubator to have a sterilization function.

[0010] The features and advantages of the present invention will be described in detail through embodiments with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 This is the main view of the multifunctional cell culture box of the utility model.

[0012] In the figure: 1-incubator body, 2-air inlet pipe, 3-gas processing device, 4-blower, 10-door, 11-storage plate, 12-ultraviolet germicidal lamp, 20-air blowing pipe, 21-straight pipe, 22-annular pipe, 25-first branch pipe, 26-air supply device, 30-outlet pipe, 40-second branch pipe, 41-first connecting pipe, 42-second connecting pipe. DETAILED DESCRIPTION

[0013] See Figure 1 The multifunctional cell culture incubator of the present invention includes an incubator body 1, a storage plate 11, an air inlet pipe 2, an air blowing pipe 20, and an air outlet pipe 30. The incubator body 1 is provided with a plurality of horizontal storage plates 11. The storage plates 11 are arranged in sequence from top to bottom. An air outlet area is formed between the lowest storage plate 11 and the bottom wall of the incubator body 1. Several storage areas are formed between adjacent storage plates 11 and between the topmost storage plate 11 and the top wall of the incubator body 1. Each storage area is provided with an ultraviolet sterilization lamp 12 and a horizontally arranged air blowing pipe 20. The air blowing pipe 20 is provided with a plurality of air blowing holes.

[0014] The air inlet pipe 2 is inserted into the interior of the incubator body 1 from the top of the incubator body 1. The air inlet pipe 2 is connected to all the air blowing pipes 20. The air inlet pipe 2 passes through all the storage plates 11 except the bottom storage plate 11. The air inlet pipe 2 is connected to a plurality of first branch pipes 25. Each first branch pipe 25 is connected to an air supply device 26. Different air supply devices 26 are used to transport different gases. The gases transported by the delivery devices 26 generally include oxygen, carbon dioxide, filtered air, acetylene oxide, etc.

[0015] The incubator body 1 is connected to a first connecting pipe 41, which is connected to the air outlet area. The first connecting pipe 41 is connected to the air inlet end of a fan 4 located outside the incubator body 1, and the air outlet end of the fan 4 is connected to a second connecting pipe 42. The second connecting pipe 42 is connected to a plurality of second branch pipes 40, and the second branch pipes 40 correspond to the storage areas one by one and are connected to the corresponding storage areas. The incubator body 1 is connected to an air outlet pipe 30, which is connected to the air outlet area. The air outlet pipe 30 is connected to the air intake end of a gas processing device 3 located outside the incubator body 1 and having an active air intake function. The first branch pipe 25, the air inlet pipe 2, and the air outlet pipe 30 are all connected to electric valves.

[0016] In order to facilitate the control of the device, the incubator body 1 includes a heating module with a temperature control function, a gas detector and a control device. The control device is electrically connected to the heating module, the gas detector and all the electric valves.

[0017] In order to ensure the fluidity and mixing uniformity of the gas, a door 10 is provided on one side of the incubator body 1 , and a storage plate 11 is fixed to the three inner walls of the incubator body 1 , with a gap between the storage plate 11 and the door 10 .

[0018] The air blowing pipe 20 is fixed on the inner wall of the incubator body 1 away from the door 10 , and the air inlet end of the first connecting pipe 41 and the air outlet end of the second branch pipe 40 are both located on the side of the incubator body 1 away from the door 10 .

[0019] In order to avoid the situation where the gas previously filled is difficult to be discharged when switching to fill gas, the air inlet pipe 2 is composed of a straight pipe 21 and an annular pipe 22. A straight pipe 21 is connected to the annular pipe 22, and the straight pipe 21 is inserted into the incubator body 1. The first branch pipe 25 is fixed on the annular pipe 22, and the first branch pipe 25 is connected to the annular pipe 22.

[0020] Working process of this utility model:

[0021] During operation of the multifunctional cell culture incubator of the present invention, taking the incubator as an example in which carbon dioxide is filled when cell culture is carried out, and then sterilized and disinfected using acetylene oxide and an ultraviolet germicidal lamp 12 after the cell culture is taken out, the culture dish is first placed on the placement plate 11, and then the door 10 is closed. The air supply device 26 for supplying carbon dioxide and the corresponding first branch pipe 25 are opened, and carbon dioxide enters the incubator body 1. The air outlet pipe 30 and the gas treatment device 3 are opened, and part of the original air is discharged through the air outlet pipe 30, so that the carbon dioxide concentration in the incubator body 1 is increased. At the same time, the fan 4 is turned on, and the gas in the incubator body 1 is rapidly circulated, so that the carbon dioxide can be quickly and fully mixed with the air, so that the carbon dioxide in the incubator body 1 can be quickly and evenly distributed. The gas detector detects the carbon dioxide concentration in real time. When the carbon dioxide concentration stabilizes at the target value, the air supply device 26 for supplying carbon dioxide and the corresponding first branch pipe 25 are closed under the control of the control device, and the air outlet pipe 30 and the gas treatment device 3 are closed. The cells are cultured. If necessary, the temperature of the incubator body 1 can be controlled by the heating module under the control of the control device.

[0022] After the culture is completed, open the door 10, take out the culture dish, then close the door, turn on the ultraviolet sterilization lamp 12, open the air supply device 26 for supplying acetylene oxide and the corresponding first branch pipe 25, open the air outlet pipe 30 and the gas treatment device 3 during the process of introducing acetylene oxide, close the air outlet pipe 30 and the gas treatment device 3 after a period of time, close the air supply device 26 for supplying acetylene oxide and the corresponding first branch pipe 25, then turn on the fan 4 to fully distribute the acetylene oxide in the incubator body 1 to accelerate the sterilization and disinfection speed, turn off the ultraviolet sterilization lamp 12 after a period of time, open the air supply device 26 for supplying filtered air and the corresponding first branch pipe 25, and open the air outlet pipe 30 and the gas treatment device 3 at the same time, and the gas detector detects the acetylene oxide concentration in real time. When the acetylene oxide concentration stabilizes at less than the allowable value, the air supply device 26 and the corresponding first branch pipe 25 for filtered air are closed under the control of the control device, and the air outlet pipe 30 and the gas treatment device 3 are closed to complete the sterilization and disinfection.

[0023] The above embodiments are intended to illustrate the present invention, not to limit the present invention. Any solution that is a simple transformation of the present invention falls within the scope of protection of the present invention.

Claims

1. Multifunctional cell culture incubator, characterized by: The incubator comprises an incubator body (1), a storage plate (11), an air inlet pipe (2), an air blowing pipe (20), and an air outlet pipe (30). The incubator body (1) is provided with a plurality of horizontally arranged storage plates (11). The storage plates (11) are arranged in sequence from top to bottom. An air outlet area is formed between the lowest storage plate (11) and the bottom wall of the incubator body (1). An air outlet area is formed between adjacent storage plates (11) and between the top storage plate (11) and the top wall of the incubator body (1). The incubator body (1) is provided with a plurality of storage areas, each of which is provided with an ultraviolet sterilization lamp (12) and a horizontally arranged air blowing pipe (20), and the air blowing pipe (20) is provided with a plurality of air blowing holes. The air inlet pipe (2) is inserted into the interior of the incubator body (1) from the top of the incubator body (1), and the air inlet pipe (2) is connected to all the air blowing pipes (20). The air inlet pipe (2) passes through all the storage plates (11) except the bottom storage plate (11), and the air inlet pipe (2) is connected to the storage plate (11). There are a plurality of first branch pipes (25), each of which is connected to an air supply device (26), and a first connecting pipe (41) is connected to the incubator body (1), the first connecting pipe (41) is connected to the air outlet area, the first connecting pipe (41) is connected to the air inlet end of a fan (4) located outside the incubator body (1), the air outlet end of the fan (4) is connected to a second connecting pipe (42), and the second connecting pipe (42) is connected to a plurality of second branch pipes (40 ), the second branch pipe (40) corresponds to the storage area one by one, the second branch pipe (40) is connected to the corresponding storage area, the incubator body (1) is connected to an air outlet pipe (30), the air outlet pipe (30) is connected to the air outlet area, the air outlet pipe (30) is connected to the air suction end of a gas processing device (3) located outside the incubator body (1) and having an active air suction function, and the first branch pipe (25), the air inlet pipe (2), and the air outlet pipe (30) are all connected to an electric valve.

2. The multifunctional cell culture incubator according to claim 1, wherein: One side of the incubator body (1) is a door (10), the placement plate (11) is fixed to the three inner walls of the incubator body (1), and there is a gap between the placement plate (11) and the door (10).

3. The multifunctional cell culture incubator according to claim 1, wherein: The air blowing pipe (20) is fixed to the inner wall of the incubator body (1) away from the door (10), and the air inlet end of the first connecting pipe (41) and the air outlet end of the second branch pipe (40) are both located on the side of the incubator body (1) away from the door (10).

4. The multifunctional cell culture incubator according to claim 1, wherein: The air inlet pipe (2) is composed of a straight pipe (21) and an annular pipe (22). The annular pipe (22) is connected to a straight pipe (21). The straight pipe (21) is inserted into the incubator body (1). The first branch pipe (25) is fixed on the annular pipe (22). The first branch pipe (25) is connected to the annular pipe (22).

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