Experimental device for carrying out dip dyeing transformation on multiple groups of plants at one time
By designing a multi-group plant immersion experimental device, the problem of processing a small number of plants each time and requiring manual operation in the existing technology is solved, and multiple groups of plants can be immersed at the same time, which improves the experimental efficiency and safety.
Patent Information
- Application Number
- CN202422757307.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-10-03
- Estimated Expiration
- 2034-11-12
AI Technical Summary
The existing plant flower soaking transformation method can only process a small number of plants at a time. The operation is cumbersome, requires manual timing and turning, and lacks systematicity.
An experimental device consisting of a dyeing box and a pull-out dyeing box was designed to support the simultaneous dyeing of multiple groups of plants. Combined with an automatic drainage system and a time controller, it eliminates the need for manual turning and timing.
It enables multiple groups of plants to be infected simultaneously, saving time and effort, improving experimental efficiency, ensuring transformation effects and increasing safety.
Smart Images
Figure CN223404958U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of plant genetic transformation experiments, in particular to an experimental device for carrying out infection and transformation of multiple groups of plants at one time. Background Art
[0002] Genetic transformation methods are essential for analyzing gene function and improving crop traits. With the development of transgenic technology, a growing number of methods for genetic transformation using exogenous genes have emerged. Agrobacterium-mediated floral-dip transformation is currently the most commonly used experimental technique for low-lying plant transformation. It is a relatively simple transgenic method that does not rely on tissue culture and subculture. This method's advantage lies in its ability to bypass tissue culture and subculture. Bolting, flowering inflorescences are used as transgene recipients for infection. The inflorescences are immersed in an Agrobacterium-containing infiltration solution for several minutes, manually timed and stirred to thoroughly infect all slightly whitish flower buds. The infiltration solution is then removed and incubated in the dark.
[0003] However, the experimental equipment used in the traditional flower soaking transformation method has a single function and can only soak a small number of plants at a time. In addition, manual turning and individual timing are required in the middle to ensure complete soaking.
[0004] In addition, the existing experimental equipment used for plant transformation using the floral immersion method is a more traditional disc-shaped container. Due to space limitations, only a few plants can be transformed at a time, and manual timing, turning, and waste liquid cleaning are required. The operating equipment is not systematic. Utility Model Content
[0005] In response to the above problems, the purpose of the present utility model is to provide an experimental device for carrying out the transformation of multiple groups of plants at one time. It can be used to transform multiple groups of potted seedlings at one time, saving time and labor, and eliminating the need for manual timing, turning and cleaning of waste liquid.
[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions:
[0007] The utility model discloses an experimental device for carrying out impregnation transformation of multiple groups of plants at one time, comprising an impregnation box; the impregnation box is a box structure with an open top and a closed bottom; a drainage system is provided on one side of the impregnation box, and a plurality of impregnation boxes are respectively provided on the remaining sides of the impregnation box so as to be pullable, and the plurality of impregnation boxes are staggered in the height direction of the impregnation box; impregnation liquid is provided in the impregnation box; the drainage system is used to discharge the impregnation liquid in the impregnation box after impregnation is completed.
[0008] In the experimental device, preferably, the inner bottom of the dyeing box has an inclination, so that when the dyeing box is pushed into the dyeing box, the drainage outlet of the drainage system docks with the lowest point of the dyeing box.
[0009] In the experimental device, preferably, the drainage system includes a main pipe and several branch pipes; the several branch pipes respectively pass through the dyeing box and dock with the lowest point of the corresponding dyeing box; and the several branch pipes are respectively connected to the main pipe.
[0010] In the experimental device, preferably, a time controller is provided on the main pipeline, and the time controller is used to set the immersion time and control the on-off of the main pipeline according to the immersion time.
[0011] The experimental device is preferably provided with a downward protruding plate on the inner top of the dipping box, so that when several dipping boxes are pushed into the dipping box, the several dipping boxes abut and press against each other, and avoid the potted seedlings placed in the dipping boxes.
[0012] The experimental device preferably further comprises a top cover; the top cover covers the top opening of the impregnation box.
[0013] In the experimental device, preferably, a liquid level line is provided in the dyeing box, and the liquid level line is used to ensure that the dyeing liquid sufficiently covers the plants without overflowing.
[0014] The utility model has the following advantages due to the adoption of the above technical solution:
[0015] The utility model adopts the arrangement of multi-layer pull-out dipping containers, which can be used to dip multiple groups of seedlings in pots at one time, thus saving time and labor. The arrangement of thickened liquid level lines and the bottom plate of the dipping box ensures the adequacy of plant transformation experiments. The arrangement of an automatic drainage system avoids the leakage of toxic waste liquid, thereby improving safety.
[0016] The utility model can replace the traditional experimental vessel for immersion transformation, provide operators with a more convenient experimental device during large-scale transformation experiments, save time and improve efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiment below. The accompanying drawings are for illustration purposes only and are not to be considered as limiting the present invention. Throughout the accompanying drawings, the same reference numerals are used to denote the same components. In the accompanying drawings:
[0018] Figure 1 It is a structural diagram of the present utility model.
[0019] The reference numerals in the figures are:
[0020] 1-dyeing box; 2-top cover; 3-time controller; 4-main pipeline; 5-first dyeing box; 6-second dyeing box; 7-third dyeing box; 8-protruding plate. DETAILED DESCRIPTION
[0021] Exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. Rather, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.
[0022] The utility model provides an experimental device for carrying out the dipping and transformation of multiple groups of plants at one time. By arranging a multi-layer integrated pull-out dipping box, multiple groups of seedling pots can be placed to dip plants at the same time; each layer of the dipping box is designed with a liquid level line that is sufficient to cover the plants without overflowing water according to the height of the plants falling sideways in the actual transformation experiment, and the top plate in the dipping box is bulged downwardly with thickening, which can gently press the plants downward so that the raised plant buds are in full contact with the dipping liquid without crushing or damaging the plants, and no manual turning is required; the bottom surface of each layer of the dipping box is set at a certain slope for easy drainage, and a drainage port is set on the side to be connected to the main pipeline. An automatic timed drainage system is installed to realize automatic drainage and collection of toxic waste liquid after dipping.
[0023] like Figure 1 As shown, the experimental device provided by the present invention for performing multiple groups of plant transformation by impregnation at one time includes an impregnation box 1; the impregnation box 1 is a box structure with an open top and a closed bottom; a drainage system is provided on one side of the impregnation box 1, and a plurality of impregnation boxes are respectively provided on the other sides of the impregnation box 1, wherein: Figure 1 In the example shown, there are three dipping boxes, namely a first dipping box 5, a second dipping box 6 and a third dipping box 7; the dipping boxes are staggered in the height direction of the dipping box; the dipping boxes are filled with dipping liquid; the drainage system is used to discharge the dipping liquid in the dipping boxes after dipping is completed.
[0024] In the above embodiment, preferably, the inner bottom of the dyeing box has an inclination, so that when the dyeing box is pushed into the dyeing box 1, the drainage outlet of the drainage system docks with the lowest point of the dyeing box, thereby facilitating the discharge of the dyeing liquid.
[0025] In the above embodiment, the drainage system preferably includes a main pipe 4 and several branch pipes; the several branch pipes respectively pass through the impregnation box 1 and connect to the lowest point of the corresponding impregnation box; and the several branch pipes are respectively connected to the main pipe 4. In this way, the impregnation liquid in the impregnation boxes at different levels can be discharged through the main pipe 4 and the branch pipes.
[0026] In the above embodiment, preferably, a time controller 3 is provided on the main pipeline 4, and the time controller 3 is used to set the soaking time and control the on and off of the main pipeline 4 according to the soaking time.
[0027] In the above embodiment, preferably, the inner top surface of the dipping box is provided with a downward protruding plate 8, so that when several dipping boxes are pushed into the dipping box, the several dipping boxes abut and press against each other, and avoid the potted seedlings placed in the dipping boxes.
[0028] In the above embodiment, preferably, the present invention further includes a top cover 2 ; the top cover 2 covers the top opening of the dyeing box 1 .
[0029] In the above embodiment, preferably, a liquid level line (not shown in the figure) is provided in the dyeing box, and the liquid level line is used to ensure that the dyeing liquid sufficiently covers the plants without overflowing.
[0030] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.
Claims
1. An experimental device for performing multiple plant transformations at once, characterized in that: Including dip-dye box; The dyeing box is a box structure with an open top and a closed bottom; A drainage system is provided on one side of the impregnation box, and a plurality of impregnation boxes are respectively provided on the other sides of the impregnation box so as to be retractable. The plurality of impregnation boxes are staggered in the height direction of the impregnation box; the impregnation boxes contain impregnation liquid; The drainage system is used to discharge the dyeing liquid in the dyeing box after the dyeing is completed.
2. The experimental device according to claim 1, characterized in that The inner bottom of the dyeing box has an inclination, and when the dyeing box is pushed into the dyeing box, the drainage port of the drainage system docks with the lowest point of the dyeing box.
3. The experimental device according to claim 1, characterized in that The drainage system includes a main pipe and several branch pipes; the several branch pipes respectively pass through the dyeing box body and connect with the lowest point of the corresponding dyeing box; Several branch pipelines are connected to the main pipeline respectively.
4. The experimental device according to claim 3, characterized in that The main pipeline is provided with a time controller, which is used to set the soaking time and control the on-off of the main pipeline according to the soaking time.
5. The experimental device according to claim 1, characterized in that: The inner top of the dipping box is provided with a downward protruding plate, so that when a plurality of the dipping boxes are pushed into the dipping box, the plurality of the dipping boxes abut and press against each other, and avoid the potted seedlings placed in the dipping boxes.
6. The experimental device according to claim 1, characterized in that It also includes a top cover; the top cover is closed at the top opening of the impregnation box.
7. The experimental device according to claim 1, characterized in that A liquid level line is provided in the dyeing box, and the liquid level line is used to ensure that the dyeing liquid sufficiently covers the plants without overflowing.