Auxiliary device suitable for low-temperature circulation dynamic dialysis

Abstract

The utility model discloses an auxiliary device suitable for low-temperature circulation dynamic dialysis, and relates to the technical field of dialysis equipment, the auxiliary device comprises a stirring shaft, a plurality of supporting rod groups are arranged on the stirring shaft, each supporting rod group comprises a plurality of supporting rods, the supporting rods are arranged on the peripheral side face of the stirring shaft in an array mode, and the supporting rods are rotatably connected with the stirring shaft; and the plurality of hollow cages are arranged on the supporting rod in an array manner. According to the auxiliary device suitable for low-temperature circulation dynamic dialysis, the technical problems that according to an existing dialysis device, multiple samples are subjected to partition dialysis at the same time, a large number of samples are likely to be mixed and cannot be distinguished are solved, different dialysis bags are placed in the corresponding hollow cages, the multiple samples can be independently partitioned and subjected to dynamic dialysis at the same time, and the dialysis efficiency is improved. The risk of cross contamination caused by sample mixing and sample liquid leakage is effectively avoided, the movement range of the dialysis bag is limited through the hollowed-out cage, the dialysis bag is prevented from cracking in the dialysis process, and it is ensured that an experiment is conducted smoothly.

Description

Technical Field

[0001] The utility model relates to the technical field of dialysis equipment, in particular to an auxiliary device suitable for low-temperature circulation dynamic dialysis. Background Art

[0002] Currently, low-temperature circulating dialysis is a common experimental method for separating and purifying proteins in the laboratory, removing excess salts, impurities, and other small molecules from protein solutions. This method is particularly suitable for replacing dialysate for long-term storage of target proteins. Dialysis is performed on the target protein sample solution. There are two types of dialysis: static dialysis and dynamic dialysis. Dynamic dialysis, which is commonly used in experiments, is more efficient and can quickly achieve salt ion equilibrium between the sample solution inside and outside the dialysis bag. The solution outside the dialysis bag is replaced several times until the salt ion concentration of the protein sample solution inside the dialysis bag meets the experimental requirements.

[0003] On the one hand, the number of sample types involved in the R&D stage of project development is large and the volume is small. Dozens or even hundreds of different project protein samples will be dialyzed simultaneously. Conventional dialysis devices now perform partitioned dialysis on multiple samples at the same time, which can easily lead to a large number of samples being confused and unable to be distinguished, resulting in the termination of the experiment. In addition, multiple dialysis bags are often mixed together during the dialysis process, making them difficult to separate. Improper operation of the separation process can cause the dialysis bag to leak, resulting in sample contamination. Directly placing the dialysis bag in dynamic dialysis will also cause it to float above the liquid surface, causing the dialysis bag to dry and crack during the dialysis process. After drying and cracking, the protein sample solution will leak and contaminate, causing the experiment to terminate. On the other hand, when the existing dialysis device adopts the upper stirring process for stirring, the stirring paddle is easily entangled with the dialysis bag. When the lower stirring process is used for stirring, the rotor will deviate, and long-term dynamic dialysis cannot be achieved well, which seriously affects the smooth progress of key project experiments. Therefore, an auxiliary device suitable for low-temperature circulation dynamic dialysis is proposed to solve the above problems. Utility Model Content

[0004] The purpose of the utility model is to provide an auxiliary device suitable for low-temperature cyclic dynamic dialysis, which solves the technical problems that conventional dialysis devices perform partitioned dialysis on multiple samples at the same time, which easily leads to confusion of a large number of samples and makes it impossible to distinguish them, and that directly placing the dialysis bag will cause the sample to float above the liquid surface, resulting in leakage and contamination of the protein sample solution, which in turn causes the experiment to be terminated.

[0005] To achieve the above-mentioned object, the present invention provides an auxiliary device suitable for low-temperature circulatory dynamic dialysis, comprising:

[0006] A stirring shaft, wherein a plurality of support rod groups are provided on the stirring shaft, wherein the support rod groups include: support rods, wherein a plurality of support rods are arranged in an array on the outer peripheral side of the stirring shaft, and the support rods are rotatably connected to the stirring shaft;

[0007] A hollow cage, wherein a plurality of the hollow cages are arranged in an array on the support rod, and the hollow cages are used to place dialysis bags.

[0008] Preferably, the upper end of the hollow cage is rotatably connected to a hollow cover, and the hollow cover is used to seal the hollow cage.

[0009] Preferably, two limiting cones are symmetrically arranged on the stirring shaft, and the tips of the two limiting cones are both facing the support rod.

[0010] Preferably, the outer side wall of the stirring shaft is connected to the first end of the torsion spring, and the first end of the torsion spring is connected to the outer side wall of the support rod.

[0011] Preferably, a first fixing buckle is provided at the edge of the hollow cover, and a second fixing buckle is provided on the outer side wall of the upper end of the hollow cage, and the first fixing buckle is snap-connected to the second fixing buckle.

[0012] Preferably, the outer side wall of the upper end of the hollow cage is rotatably connected to a handle, and the handle is used to assist in lifting the hollow cage.

[0013] Preferably, a plurality of fasteners are arranged in an array on the support rod, and the fasteners are connected to the handle.

[0014] Preferably, a snap ring is provided on the handle, and the snap ring is used to fix the position of the handle on the support rod.

[0015] Preferably, a shaft seal is provided at the top end of the stirring shaft, and the shaft seal is connected to a driving device.

[0016] Preferably, a label is provided on the hollow cover, and the label displays the sample identification.

[0017] Compared with the above background technology, the present invention provides an auxiliary device suitable for low-temperature circulatory dynamic dialysis, which has the following beneficial effects:

[0018] (1) By placing different dialysis bags in corresponding hollow cages and driving each hollow cage to rotate via the stirring shaft, multiple samples can be independently partitioned and dynamically dialyzed simultaneously, effectively avoiding the risk of sample confusion and cross-contamination caused by sample leakage, and effectively improving the effect of dynamic dialysis. On the other hand, the hollow cage limits the range of movement of the dialysis bag, allowing the dialysis bag to always be immersed below the liquid surface, thereby preventing the dialysis bag from drying out and cracking during the dialysis process, avoiding spillage and contamination of the sample solution, and further ensuring the smooth progress of the experiment.

[0019] (2) By fixing the hollow cage at different positions of the support rod, independent rotation of each sample can be realized, and the phenomenon that the stirring paddle is wound around the dialysis bag and the stirring paddle collides with the dialysis bag to cause the rotor to deviate is avoided, and the risk that the stirring paddle scratches the dialysis bag is avoided, so that long-term dynamic dialysis can be better realized, and the smooth development of the experiment is further ensured. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to more clearly illustrate the technical scheme in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are only embodiments of the present application, and for those skilled in the art, other drawings can be obtained without creative labor on the basis of the provided drawings.

[0021] Figure 1 A perspective view of the auxiliary device provided by the embodiments of the present application is shown.

[0022] Figure 2 A perspective view of the hollow cage provided by the embodiments of the present application is shown.

[0023] Figure 3 An assembly view of the stirring shaft and the support rod provided by the embodiments of the present application is shown.

[0024] Specifically, 1-stirring shaft; 2-support rod; 3-hollow cage; 4-shaft seal; 5-hollow cover; 6-first fixing buckle; 7-second fixing buckle; 8-rivets; 9-limiting cone; 10-buckling part; 11-lifting handle; 12-buckling ring; 13-signboard. DETAILED DESCRIPTION

[0025] The technical scheme in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0026] In order to enable those skilled in the art to better understand the technical scheme of the present application, the present application will be further described in detail in combination with the drawings and specific embodiments.

[0027] As shown in Figure 1 and Figure 2 A kind of auxiliary device suitable for low temperature cycle dynamic dialysis, including: stirring shaft 1, support rod 2 group and hollow cage 3.

[0028] Several groups of support rods 2 are arranged on the stirring shaft 1, wherein a shaft seal 4 is provided at the top end of the stirring shaft 1, the shaft seal 4 is connected to the driving device, and the driving device drives the stirring shaft 1 to rotate through the shaft seal 4. Specifically, two groups of support rods 2 are provided, and the two groups of support rods 2 are arranged on the stirring shaft 1 from top to bottom in sequence. The support rod 2 group includes several support rods 2 arranged in an array on the outer peripheral side of the stirring shaft 1.

[0029] Specifically, each support rod group 2 includes two cross-arranged support rods 2, each of which is rotatably connected to the stirring shaft 1. Furthermore, the support rods 2 can swing up and down a certain angle relative to the stirring shaft 1, that is, the support rods 2 can swing at a certain angle within the vertical plane. Furthermore, the support rods 2 within the same support rod group 2 are connected to the stirring shaft 1 at equal angles, ensuring that the hollow cages 3 on different support rods 2 and the same support rod 2 do not contact each other, effectively preventing collisions between the dialysis bags.

[0030] Preferably, four hollow cages 3 are arranged in an array on each support rod 2, and different dialysis bags are placed in the corresponding hollow cages 3, wherein the outer wall of the hollow cage 3 is hollow, which does not affect the contact between the dialysis bag and the dialysate in the container. Its main purpose is to limit the range of movement of the dialysis bag. By driving each hollow cage 3 to rotate through the stirring shaft 1, multiple samples can be independently partitioned and dynamic dialysis can be performed simultaneously, effectively avoiding the risk of sample confusion and cross contamination caused by sample leakage, and effectively improving the effect of dynamic dialysis.

[0031] During use, multiple dialysis bags containing protein samples to be dialyzed are placed in different hollow cages 3 in sequence, the hollow cages 3 are fixed to the support rods 2, and then the stirring shaft 1 is inserted into a container filled with dialysate. It should be noted that when the hollow cage 3 is at the highest point, the hollow cage 3 can still be immersed in the dialysate, and the stirring shaft 1 rotates under the drive of the driving device, driving the protein sample in the hollow cage 3 to perform dynamic dialysis. By fixing the hollow cage 3 at different positions of the support rods 2, each sample can be rotated independently, and there will be no phenomenon of the stirring paddle entangled with the dialysis bag or the stirring paddle colliding with the dialysis bag causing the rotor to deviate, effectively avoiding the risk of the stirring paddle scratching the dialysis bag and ensuring the smooth progress of the experiment.

[0032] The upper end of the hollow cage 3 is rotatably connected to the hollow cover 5, which is used to seal the hollow cage 3. After the hollow cover 5 is buckled with the hollow cage 3, the range of movement of the dialysis bag is limited to the hollow cage 3 during the process of the stirring shaft 1 driving the hollow cage 3 to rotate, so that the dialysis bag can always be immersed below the liquid surface and will not float above the liquid surface, thereby preventing the dialysis bag from drying out and cracking during the dialysis process, avoiding leakage and contamination of the sample solution, and further ensuring the smooth progress of the experiment.

[0033] Two limiting cones 9 are symmetrically arranged on the stirring shaft 1, and the tips of the two limiting cones 9 are both facing the support rod 2. When the support rod 2 swings in the vertical plane, the outer peripheral side of the limiting cone 9 can limit the up and down swinging angle of the support rod 2. It should be noted that after the support rod 2 swings to the maximum angle, the angle between the support rod 2 and the horizontal plane is not less than 45°, which is convenient for taking and placing the hollow cage 3.

[0034] like Figure 3 As shown, a plurality of assembly holes are arranged in an array on the outer peripheral side of the stirring shaft 1, and the rivet 8 passes through the middle part of the support rod 2 to connect the assembly holes. Each assembly hole corresponds to a rivet 8. After the support rod 2 is connected by the rivet 8, the lengths of the support rods 2 on both sides of the rivet 8 are the same. When the hollow cage 3 is not hung, the support rod 2 is kept as balanced as possible.

[0035] In addition, the outer wall of the stirring shaft 1 is connected to the first end of the torsion spring (not shown in the figure), the first end of the torsion spring is connected to the outer wall of the support rod 2, and the torsion spring is coaxially arranged with the rivet 8. In the initial state, the support rod 2 maintains a horizontal state under the torsional force of the torsion spring. At this time, the support rod 2 is perpendicular to the stirring shaft 1, further keeping the support rod 2 as balanced as possible.

[0036] In one embodiment of the present invention, a first fixing buckle 6 is provided at the edge of the hollow cover 5, and a second fixing buckle 7 is provided on the outer side wall of the upper end of the hollow cage 3. The first fixing buckle 6 is snap-connected to the second fixing buckle 7. After the hollow cover 5 is snap-fitted onto the hollow cage 3, the dialysis bag is stably confined in the hollow cage 3 through the snap-connection of the first fixing buckle 6 and the second fixing buckle 7. The hollow cover 5 will not be separated from the hollow cage 3 due to the rotation of the hollow cage 3 with the stirring shaft 1 in the dialysate. Multiple samples are always kept in independent partitions, effectively avoiding the risk of sample confusion and cross-contamination caused by sample leakage.

[0037] The outer side wall of the upper end of the hollow cage 3 is rotatably connected to the handle 11, and the handle 11 assists in lifting the hollow cage 3. The two ends of the handle 11 are inserted into the outer side wall of the hollow cage 3, and the handle 11 can rotate freely relative to the hollow cage 3. Specifically, the handle 11 is a semi-ring shape as a whole. When the hollow cover 5 is buckled on the hollow cage 3, the handle 11 can still rotate from one side of the hollow cage 3 to the other side of the hollow cage 3.

[0038] A number of fasteners 10 are arranged in an array on the support rod 2, and the handle 11 is connected by the fasteners 10 to hang the hollow cage 3 on the support rod 2, instead of directly connecting the handle 11 to the support rod 2. In this way, the specified hollow cage 3 can be removed separately according to needs, which is more convenient to use.

[0039] Preferably, the fastener 10 is an elliptical fixed ring with a closed structure, directly welded to the support rod 2. Correspondingly, a snap ring 12 is provided on the handle 11. The snap ring 12 is preferably an elastic snap, which can be specifically referred to as a keychain. After the elastic snap is connected to the fixed ring, the position of the handle 11 on the support rod 2 can be simultaneously fixed, preventing the hollow cage 3 from moving back and forth on the support rod 2, thereby further avoiding the risk of sample confusion and cross-contamination caused by sample leakage. In addition, the snap ring 12 is located in the middle of the handle 11, which can ensure that the hollow cage 3 maintains a balanced state and does not tip over, ensuring the stability of the hollow cage 3 when rotating within the container.

[0040] In one embodiment of the present invention, a label 13 is provided on the hollow cover 5, and the label 13 is clearly marked on the label. Specifically, the project name can be written on the label 13 with a marker pen. Compared with the traditional paper label pasted on the dialysis bag, it can be soaked in the dialysate for a long time without falling off, which is convenient for distinguishing various dialysis bags in the later stage, further avoiding sample confusion, effectively improving the effect of dynamic dialysis, and ensuring the smooth progress of the experiment.

[0041] It should be noted that the auxiliary device can directly connect multiple hollow cages 3 to the dialysis bag and transfer them as a whole, facilitating direct replacement of the dialysate without affecting the samples, making the overall experimental process more convenient and faster. Furthermore, the multi-layered support rods 2 can hang more hollow cages 3, allowing large numbers of samples to be dialyzed and replaced with dialysate simultaneously in the same space, effectively saving space resources.

[0042] Furthermore, in the overall device, all components such as the stirring shaft 1, support rod 2 and hollow cage 3 are ground and polished. On the one hand, it can prevent the components from touching the dialysis bag and cutting the dialysis bag, causing it to be damaged and leaked, affecting the experiment. On the other hand, it can play a certain safety protection role and improve the safety of the experimenter's operation.

[0043] When the utility model is used, multiple dialysis bags containing protein samples to be dialyzed are placed in different hollow cages 3 in turn, the hollow cover 5 is buckled on the hollow cage 3, and the hollow cover 5 is further locked and fixed by the first fixing buckle 6 and the second fixing buckle 7. The fasteners 10 on the hollow cage 3 are connected to the fasteners 10, and the hollow cage 3 is fixed on the support rod 2. Then the stirring shaft 1 is connected to each hollow cage 3 and inserted into the container filled with dialysate. The stirring shaft 1 rotates under the drive of the driving device, driving the protein sample in the hollow cage 3 to perform dynamic dialysis.

[0044] In summary, by placing different dialysis bags in corresponding hollow cages 3 and driving each hollow cage 3 to rotate via the stirring shaft 1, multiple samples can be independently partitioned and dynamically dialyzed simultaneously, effectively avoiding the risk of sample confusion and cross-contamination caused by sample leakage, and improving the effectiveness of dynamic dialysis. Furthermore, the ability to achieve independent rotation of each sample while avoiding the risk of the stirring paddle scratching the dialysis bag allows for long-term dynamic dialysis, ensuring the smooth conduct of the experiment. The overall device has higher repeatability and controllability.

[0045] It should be noted that, in this specification, relational terms such as first and second are merely used to distinguish one entity from other entities, but do not necessarily require or imply any actual relationship or order between these entities.

[0046] This document uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only intended to help understand the method and core concept of the present invention. It should be noted that those skilled in the art can make various improvements and modifications to the present invention without departing from the principles of the present invention, and such improvements and modifications also fall within the scope of protection of the present invention.

Claims

1. An auxiliary device suitable for low-temperature cyclic dynamic dialysis, characterized in that: include: A stirring shaft, wherein a plurality of support rod groups are provided on the stirring shaft, wherein the support rod groups include: support rods, wherein a plurality of support rods are arranged in an array on the outer peripheral side of the stirring shaft, and the support rods are rotatably connected to the stirring shaft; A hollow cage, wherein a plurality of the hollow cages are arranged in an array on the support rod, and the hollow cages are used to place dialysis bags.

2. The auxiliary device for low-temperature circulatory dynamic dialysis according to claim 1, characterized in that: The upper end of the hollow cage is rotatably connected to a hollow cover, and the hollow cover is used to seal the hollow cage.

3. The auxiliary device for low-temperature cyclic dynamic dialysis according to claim 2, characterized in that: Two limiting cones are symmetrically arranged on the stirring shaft, and the tips of the two limiting cones are both facing the support rod.

4. The auxiliary device for low-temperature cyclic dynamic dialysis according to claim 2, characterized in that: The outer side wall of the stirring shaft is connected to the first end of the torsion spring, and the first end of the torsion spring is connected to the outer side wall of the support rod.

5. An auxiliary device suitable for low-temperature cyclic dynamic dialysis according to any one of claims 2 to 4, characterized in that: A first fixing buckle is provided at the edge of the hollow cover, and a second fixing buckle is provided on the outer side wall of the upper end of the hollow cage, and the first fixing buckle is connected to the second fixing buckle by snapping.

6. The auxiliary device for low-temperature cyclic dynamic dialysis according to claim 5, characterized in that: The outer side wall of the upper end portion of the hollow cage is rotatably connected to a handle, and the handle is used to assist in lifting the hollow cage.

7. The auxiliary device for low-temperature cyclic dynamic dialysis according to claim 6, characterized in that: A plurality of fasteners are arranged in an array on the support rod, and the fasteners are connected to the handle.

8. The auxiliary device for low-temperature circulatory dynamic dialysis according to claim 7, characterized in that: A snap ring is provided on the handle, and the snap ring is used to fix the position of the handle on the support rod.

9. An auxiliary device suitable for low-temperature cyclic dynamic dialysis according to any one of claims 1 to 4, characterized in that: A shaft seal is provided at the top end of the stirring shaft, and the shaft seal is connected to a driving device.

10. An auxiliary device suitable for low-temperature cyclic dynamic dialysis according to any one of claims 2 to 4, characterized in that: A signboard is provided on the hollow cover, and the sample identification is displayed on the signboard.

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