Sample bottle for streptomyces darkness strain breeding
By adopting a conical wall and vertical wall design in the sample bottle for dark Streptomyces strain selection, combined with anti-backflow and fixed components, the problem of uneven mixing of solutions during centrifugation or shaking is solved, thereby improving the accuracy and efficiency of the experiment.
Patent Information
- Application Number
- CN202423008207.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-06
- Publication Date
- 2025-10-10
- Estimated Expiration
- 2034-12-06
AI Technical Summary
During the strain selection process of Streptomyces pyogenes, the solution flows upward toward the vertical wall due to centrifugal force during centrifugation or shaking, resulting in uneven mixing and affecting the accuracy of experimental results and work efficiency.
A sample bottle including a conical wall and a vertical wall is designed, combined with an anti-backflow component and a fixing component. The angle between the conical wall and the vertical wall is used to limit the flow of the solution to prevent the solution from flowing back, and the sealing of the bottle body is ensured by the limiting groove and the fixing component.
It solves the problem of uneven solution mixing, improves the efficiency and convenience of the sample bottle, ensures the stability and controllability of the solution during centrifugation or shaking, and avoids solution leakage and external contamination.
Smart Images
Figure CN223422670U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of sample bottles, in particular to a sample bottle for breeding dark Streptomyces strains. Background Art
[0002] Dark Streptomyces is a prokaryotic organism of the Streptomycete family and the genus Streptomyces. Its spore hyphae are loose spiral to wavy, and its spores are oblong to cylindrical with a smooth surface. They were isolated from Mexican soil samples. During the strain selection process, it is often necessary to move or transport the sample bottles. Traditional sample bottles are easy to tip over during transportation or movement, while the sample bottles for strain selection have a design to prevent tipping. During the breeding process, dark Streptomyces needs to grow and reproduce under specific conditions. Any slight environmental change affects its growth and variation. The sample bottles for strain selection usually have good sealing and stability. When breeding dark Streptomyces, it is necessary to frequently add or remove substances to the sample bottles for inoculation, sampling, testing and other operations. The design of the sample bottles for strain selection facilitates these operations for staff and improves work efficiency.
[0003] The existing sample bottles for the selection and breeding of dark Streptomyces meet the selection and breeding needs of dark Streptomyces through design and the realization of multiple functions. These sample bottles first provide an environment suitable for the growth of dark Streptomyces, including having a suitable capacity to accommodate an appropriate amount of culture medium and strains, a unique shape that helps to evenly distribute the culture medium and strains, and good sealing performance to effectively prevent external contamination and evaporation of the culture medium. At the same time, they can accurately control the culture conditions, such as maintaining a suitable temperature, adjusting humidity, and providing a specific gas environment to meet the growth needs of dark Streptomyces. In terms of operation and observation, these sample bottles are easy for operators to hold and perform various operations. They have openings that are convenient for adding culture medium and strains, and are marked with clear scales or marks to facilitate accurate measurement and recording of the volume of the culture medium and the concentration of the strains. In addition, these sample bottles also have sufficient strength and stability to ensure that they will not break or leak during use, ensuring the safety of the operator.
[0004] However, in the actual use of sample bottles for dark Streptomyces strain selection, there are still some problems. One of the more prominent problems is that during the experimental operation, such as when centrifugation or shaking is required, the solution will flow upward onto the vertical wall due to the action of centrifugal force, resulting in uneven mixing of the solution in the sample bottle. The occurrence of this situation seriously affects the efficiency of the sample bottles for dark Streptomyces strain selection, causing deviations in the experimental results, and failing to accurately reflect the growth and variation of dark Streptomyces. It also brings many inconveniences to scientific researchers and, to a certain extent, hinders the smooth progress of dark Streptomyces strain selection. Utility Model Content
[0005] In order to make up for the above shortcomings, the utility model provides a sample bottle for streptomyces tenebrarius breeding, aiming at improving the problem that the solution flows up on the vertical wall because of the centrifugal force when centrifuging or shaking in the experiment process, resulting in uneven mixing of the solution.
[0006] In order to realize the above object, the utility model adopts the following technical scheme: a sample bottle for streptomyces tenebrarius breeding, including the conical wall, the conical wall bottom fixedly connected with the bottom wall, the conical wall inside is provided with the backflow prevention subassembly, the conical wall top is provided with the bottle lid, the bottle lid bottom is provided with the fixed component;
[0007] The backflow prevention subassembly includes the vertical wall and the extension wall, the vertical wall outer wall is fixedly connected in the conical wall inside, the extension wall inner wall is fixedly connected in the vertical wall top, the vertical wall top is opened in the limiting slot.
[0008] As a further description of the above technical scheme:
[0009] The fixed component includes the connecting wall and the fixed column, the connecting wall top is fixedly connected in the bottle lid bottom, the fixed column top is fixedly connected in the connecting wall bottom.
[0010] As a further description of the above technical scheme:
[0011] The fixed column top is fixedly connected with a plurality of fixed blocks, and the bottom of each fixed block is fixedly connected with a limiting strip.
[0012] As a further description of the above technical scheme:
[0013] The fixed column outer wall is fixedly connected with a plurality of upper fixed rings, and the fixed column outer wall is fixedly connected with a plurality of anti-skid rings.
[0014] As a further description of the above technical scheme:
[0015] The fixed column outer wall is fixedly connected with a plurality of lower fixed rings, and the lower fixed ring and the upper fixed ring are fixedly connected in parallel array on the fixed column outer wall.
[0016] As a further description of the above technical scheme:
[0017] The vertical wall top is provided with a sliding slot, and the limiting slot is arranged above the sliding slot.
[0018] As a further description of the above technical scheme:
[0019] The connecting wall outer wall is slidably connected in the limiting slot inside, and the fixed column outer wall is slidably connected in the vertical wall inner wall.
[0020] As a further description of the above technical solutions:
[0021] The plurality of fixed blocks are arranged in a ring array and fixedly connected at the top of the fixed column, and the limiting strips and the connecting walls are arranged in a parallel array at the top of the fixed column.
[0022] The utility model has the advantages of the following:
[0023] 1. In the utility model, the connecting part between the conical wall and the vertical wall is lengthened, so that the solution in the bottle does not flow upward into the vertical wall due to the centrifugal force during centrifugation or shaking in the experimental process, the problem of uneven mixing of the solution caused by the solution flowing upward into the vertical wall due to the centrifugal force during centrifugation or shaking in the experimental process is solved, and the efficiency of the sample bottle for dark streptomycin strain breeding is improved.
[0024] 2. In the utility model, the bottle cap is fixed by the limiting strip and the sliding groove, so that the entire bottle body is sufficiently sealed during the experimental process, and the solution does not flow out during centrifugation or shaking, the problem of the solution spilling from the bottle during centrifugation or shaking due to insufficient sealing of the bottle body during the experimental process is solved, and the convenience of the sample bottle for dark streptomycin strain breeding is improved. BRIEF DESCRIPTION OF DRAWINGS
[0025] Figure 1 A perspective view of a sample bottle for dark streptomycin strain breeding is provided for the utility model;
[0026] Figure 2 A schematic view of the internal structure of the conical wall of a sample bottle for dark streptomycin strain breeding is provided for the utility model;
[0027] Figure 3 A schematic view of the internal structure of the vertical wall of a sample bottle for dark streptomycin strain breeding is provided for the utility model.
[0028] LEGEND:
[0029] 1. Conical wall; 2. Bottom wall; 3. Vertical wall; 4. Extension wall; 5. Bottle cap; 6. Connecting wall; 7. Fixed column; 8. Limiting groove; 9. Fixed block; 10. Limiting strip; 11. Sliding groove; 12. Upper fixed ring; 13. Anti-skid ring; 14. Lower fixed ring. DETAILED DESCRIPTION
[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0031] Reference Figure 1 and Figure 2 The utility model provides an embodiment: a sample bottle for the breeding of dark Streptomyces strains, comprising a conical wall 1, which is the main component of the sample bottle, and has a conical shape and a certain structural stability. The bottom of the conical wall 1 is fixedly connected to the bottom wall 2, which plays a supporting and sealing role, ensuring that the bottom of the sample bottle is stable. The conical wall 1 is provided with an anti-backflow component inside, which can effectively prevent the solution from flowing back and ensure the normal flow and mixing of the solution in the sample bottle. The top of the conical wall 1 is provided with a bottle cap 5 for sealing the sample bottle to prevent solution leakage and external contamination. The bottom of the bottle cap 5 is provided with a fixing component, which can firmly fix the bottle cap 5 on the vertical wall 3 to ensure the sealing and stability of the sample bottle.
[0032] The anti-backflow assembly includes a vertical wall 3 and an extension wall 4. The outer wall of the vertical wall 3 is fixedly connected to the inside of the conical wall 1, playing a major blocking role to prevent the solution from flowing back upward. The inner wall of the extension wall 4 is fixedly connected to the top of the vertical wall 3 to prevent the solution from flowing out of the bottle mouth when the bottle is full. A limiting groove 8 is provided on the top of the vertical wall 3 to limit the movement range of the bottle cap 5. The fixing assembly includes a connecting wall 6 and a fixing column 7. The top of the connecting wall 6 is fixedly connected to the bottom of the bottle cap 5 for connecting the bottle cap 5 and the fixing column 7. The top of the fixing column 7 is fixedly connected to the bottom of the connecting wall 6 for connecting other fixing components.
[0033] Specifically, during the actual use of the sample bottle, when centrifugation or shaking operations are required, the angle of about 15° to 45° formed between the vertical wall 3 and the conical wall 1 cleverly limits the solution in the bottle to the range of the conical wall 1, so that it will not easily surge upward or overflow due to centrifugal force or shaking, and the solution is firmly trapped in the area of the conical wall 1, ensuring the stability and controllability of the solution during centrifugation or shaking, avoiding uneven mixing or other adverse effects of the solution due to accidental flow, and providing reliable protection for the normal use of the sample bottle.
[0034] Reference Figure 3The top of the fixing column 7 is fixedly connected with a plurality of fixing blocks 9 for connecting the fixing column 7 and the limiting strip 10. The bottom of each fixing block 9 is fixedly connected with the limiting strip 10 for fixing the bottle cap 5. The outer wall of the fixing column 7 is fixedly connected with a plurality of upper fixing rings 12 for fixing the anti-slip ring 13. The outer wall of the fixing column 7 is fixedly connected with a plurality of anti-slip rings 13 for fixing the bottle cap 5 and the vertical wall 3 and sealing the bottle body. The outer wall of the fixing column 7 is fixedly connected with a plurality of lower fixing rings 14 for fixing the anti-slip ring 13. The lower fixing ring 14 and the upper fixing ring 12 are arranged in a parallel array and fixedly connected to the outer wall of the fixing column 7. A sliding groove 11 is provided at the top of the vertical wall 3. The limiting groove 8 is provided above the sliding groove 11. The outer wall of the connecting wall 6 is slidably connected to the inner wall of the limiting groove 8. The outer wall of the fixing column 7 is slidably connected to the inner wall of the vertical wall 3. The plurality of fixing blocks 9 are arranged in a circular array and fixedly connected to the top of the fixing column 7. The limiting strip 10 and the connecting wall 6 are arranged in a parallel array at the top of the fixing column 7.
[0035] Specifically, when the bottle body needs to be sealed, the operator will first pick up the bottle cap 5, and then slowly slide the anti-slip ring 13 connected to the outer wall of the fixing column 7 into the interior of the vertical wall 3. At this time, the anti-slip ring 13 fits tightly against the inner wall of the vertical wall 3, providing a reliable anti-slip effect to prevent accidental slippage during operation. Then, the fixing block 9 and the limiting strip 10 connected to the outer wall of the fixing column 7 are accurately aligned with the opening position of the sliding groove 11. After the fixing block 9 slides smoothly into the interior, the operator will slide toward the extended side. During this process, the fixing block 9 and the limiting strip 10 move smoothly along the sliding groove 11 until the bottle cap 5 is firmly fixed as a whole, thereby achieving sealing of the bottle body.
[0036] Working principle: When using the sample bottle for breeding dark Streptomyces strains, first place the dark Streptomyces to be bred into the bottle body, and then shake the solution inside evenly. At this time, the angle formed between the vertical wall 3 and the conical wall 1 cleverly limits the solution in the bottle body to the range of the conical wall 1, so that it will not easily surge upward or overflow due to centrifugal force or shaking, and firmly trap the solution in the area of the conical wall 1, ensuring the stability and controllability of the solution during centrifugation or shaking. After that, the operator picks up the bottle cap 5, and then slides the anti-slip ring 13 connected to the outer wall of the fixed column 7 into the vertical wall 3, and then aligns the fixed block 9 and the limit strip 10 connected to the outer wall of the fixed column 7 with the opening position of the sliding groove 11. After sliding the fixed block 9 into the interior, slide toward the extended side, thereby fixing and sealing the bottle cap 5 as a whole, and then placing the sample bottle in a suitable environment for breeding.
[0037] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.
Claims
1. A sample bottle for the selection and breeding of dark Streptomyces species, comprising a tapered wall (1), characterized in that: The bottom of the conical wall (1) is fixedly connected to a bottom wall (2), an anti-backflow component is provided inside the conical wall (1), a bottle cap (5) is provided on the top of the conical wall (1), and a fixing component is provided at the bottom of the bottle cap (5); The backflow prevention assembly comprises a vertical wall (3) and an extension wall (4), wherein the outer wall of the vertical wall (3) is fixedly connected to the inside of the conical wall (1), and the inner wall of the extension wall (4) is fixedly connected to the top of the vertical wall (3), and a limiting groove (8) is provided on the top of the vertical wall (3).
2. The sample bottle for selecting and breeding dark Streptomyces according to claim 1, characterized in that: The fixing assembly comprises a connecting wall (6) and a fixing column (7), wherein the top of the connecting wall (6) is fixedly connected to the bottom of the bottle cap (5), and the top of the fixing column (7) is fixedly connected to the bottom of the connecting wall (6).
3. The sample bottle for selecting and breeding dark Streptomyces according to claim 2, characterized in that: A plurality of fixing blocks (9) are fixedly connected to the top of the fixing column (7), and a limiting strip (10) is fixedly connected to the bottom of each fixing block (9).
4. The sample bottle for selecting and breeding dark Streptomyces according to claim 2, characterized in that: The outer wall of the fixing column (7) is fixedly connected with a plurality of upper fixing rings (12), and the outer wall of the fixing column (7) is fixedly connected with a plurality of anti-slip rings (13).
5. The sample bottle for selecting and breeding dark Streptomyces according to claim 2, characterized in that: A plurality of lower fixing rings (14) are fixedly connected to the outer wall of the fixing column (7), and the lower fixing rings (14) and the upper fixing rings (12) are arranged in a parallel array and fixedly connected to the outer wall of the fixing column (7).
6. The sample bottle for selecting and breeding dark Streptomyces according to claim 1, characterized in that: A sliding groove (11) is provided on the top of the vertical wall (3), and the limiting groove (8) is provided above the sliding groove (11).
7. The sample bottle for selecting and breeding dark Streptomyces according to claim 2, characterized in that: The outer wall of the connecting wall (6) is slidably connected to the inside of the limiting groove (8), and the outer wall of the fixing column (7) is slidably connected to the inner wall of the vertical wall (3).
8. The sample bottle for selecting and breeding dark Streptomyces according to claim 3, characterized in that: The plurality of fixed blocks (9) are arranged in a circular array and fixedly connected to the top of the fixed column (7), and the limiting strips (10) and the connecting wall (6) are arranged in a parallel array and arranged on the top of the fixed column (7).