Rapid detection device for sulfate reducing bacteria
By designing a rapid detection device with a detachable bottle cap and storage cylinder, the problems of sulfate-reducing bacteria detection devices' dependence on large facilities and H2S gas overflow were solved, and real-time monitoring and safe operation of the fermentation liquid were achieved.
Patent Information
- Application Number
- CN202521815765.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-26
- Publication Date
- 2025-10-10
- Estimated Expiration
- 2035-08-26
AI Technical Summary
Existing sulfate-reducing bacteria detection devices require large facilities and are difficult to achieve real-time monitoring of fermentation broth. In addition, H2S gas easily overflows, causing environmental pollution and operational risks.
A rapid detection device consisting of a detachable bottle cap and a storage cylinder was designed. The storage cylinder was filled with an impregnated carbon column and fixed by elastic connections and positioning components to prevent H2S gas from overflowing.
Real-time monitoring and safe operation of fermentation liquid are achieved, H2S gas overflow is avoided, and the risk of environmental pollution is reduced.
Smart Images

Figure CN223422672U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of sulfate-reducing bacteria detection, in particular to a rapid detection device for sulfate-reducing bacteria. Background Art
[0002] Sulfate-reducing bacteria (SRB) are a type of anaerobic bacteria that use sulfate or other oxidized sulfides as electron acceptors to dissimilate organic matter. They have been studied and gradually applied to the treatment of acidic heavy metal-contaminated mine wastewater with significant results. The principle is that under anaerobic or minimal oxygen conditions, SRB uses organic matter or H2 as electron donors to reduce sulfate to produce -2-valent sulfide ions. The produced -2-valent sulfide ions combine with heavy metal ions in the wastewater to form metal sulfide precipitates, thereby removing sulfate and heavy metal ions from the wastewater.
[0003] Conventional laboratory sulfate-reducing bacteria (SRB) culture equipment requires large-scale facilities such as liquid nitrogen tanks, making it difficult to widely use in conventional laboratories. Furthermore, it is difficult to achieve real-time monitoring and control of the fermentation broth. To address this issue, patent document CN216404362U uses commonly used laboratory instruments. The device can be easily assembled and completed. By timely adjusting environmental conditions, suitable SRB (sulfate-reducing bacteria) fermentation conditions can be created, and experimental gas can be tested. However, if the concentration of H2S in the sulfate-reducing bacteria test bottle is too high, it is easy for the H2S to not fully react with the lead acetate reagent in the sulfate-reducing bacteria test bottle. This can lead to the leakage of toxic H2S gas when the bottle is opened to process the residue, causing environmental pollution and operational risks.
[0004] In order to solve the above problems, it is necessary to improve the structure of the sulfate-reducing bacteria detection bottle based on the structure of publication number CN216404362U to solve the above technical problems. Utility Model Content
[0005] In order to solve the above technical problems, the utility model provides a rapid detection device for sulfate-reducing bacteria.
[0006] The rapid detection device for sulfate-reducing bacteria provided by the utility model comprises a detection bottle, a detachable bottle cap is installed on the detection bottle, and a catheter is fixedly installed on the bottle cap;
[0007] The bottle cap is provided with an installation cavity, and an elastically connected storage cylinder is inserted into the installation cavity, and the storage cylinder stores the impregnated carbon column, the cylinder wall of the storage cylinder is provided with a plurality of annularly distributed through grooves, and the outer cylinder wall of the storage cylinder is provided with an elastic telescopic sleeve;
[0008] The bottle cap is also provided with a positioning assembly for positioning the storage cylinder to slide into the detection bottle depth.
[0009] Preferably, the positioning assembly includes a guide rod, which is fixedly mounted on the bottle cap and is sleeved with a slidingly connected collar, which is fixedly connected to the top of the storage cylinder and is inserted with a threaded fastening screw.
[0010] Preferably, a spring is sleeved on the storage cylinder, one end of the spring is fixedly connected to the edge of the upper surface of the storage cylinder, and the other end of the spring is fixedly connected to the upper surface of the bottle cap.
[0011] Preferably, the elastic telescopic sleeve is provided on the storage tube and covers the spring, one end of the elastic telescopic sleeve is fixedly connected to the edge of the upper surface of the storage tube, and the other end of the elastic telescopic sleeve is fixedly connected to the upper surface of the bottle cap.
[0012] Preferably, the storage barrel is equipped with an abutment assembly, which includes a sliding rod, which is inserted into the installation cavity opened in the storage barrel and is slidably connected to the installation cavity, and a supporting base plate for sealing the bottom opening of the storage barrel is installed at the bottom of the sliding rod, and a tension spring is fixedly installed on the top of the sliding rod, and the other end of the tension spring is fixedly connected to the inner top wall of the installation cavity.
[0013] Preferably, the diameter of the edge of the barrel opening at the bottom of the storage barrel is larger than the inner diameter of the installation cavity.
[0014] Preferably, a magnetic ring A is fixedly installed on the bottle mouth of the detection bottle, and a magnetic ring B is fixedly installed inside the bottle cap, and the magnetic properties of the magnetic ring B and the magnetic ring A are opposite.
[0015] Compared with related technologies, the rapid detection device for sulfate-reducing bacteria provided by the present invention has the following beneficial effects:
[0016] When the experiment is finished, the storage tube is pressed down to drive the storage tube to slide into the detection bottle. At this time, the spring is compressed. After sliding to a suitable height, the fastening screw is rotated to fix the storage tube on the guide rod, thereby locking the depth of the storage tube sliding into the detection bottle. At this time, the elastic telescopic sleeve is contracted. Since the storage tube has a through groove on its periphery, the unreacted H2S can be quickly absorbed by the impregnated carbon column in the storage tube, thereby avoiding the hidden danger of H2S overflowing after the detection bottle is opened. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 A schematic structural diagram of a preferred embodiment of the rapid detection device for sulfate-reducing bacteria provided by the present invention;
[0018] Figure 2 for Figure 1 The schematic diagram of the structure of the detection bottle and bottle cap shown;
[0019] Figure 3 for Figure 1 The schematic diagram of the cross-sectional structure of the storage cylinder when it is on the bottle cap;
[0020] Figure 4 for Figure 3 Schematic diagram of the cross-sectional structure of the storage cylinder shown.
[0021] Numbers in the figure: 1. Detection bottle; 11. Magnetic ring A; 2. Bottle cap; 2a. Mounting cavity; 21. Magnetic ring B; 22. Conduit; 3. Storage cylinder; 3a. Mounting cavity; 3b. Through groove; 4. Impregnated carbon column; 5. Abutment assembly; 51. Sliding rod; 52. Supporting base; 53. Tension spring; 6. Spring; 7. Elastic telescopic sleeve; 8. Positioning assembly; 81. Guide rod; 82. Collar; 83. Fastening screw. DETAILED DESCRIPTION
[0022] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.
[0023] The specific implementation of the present invention is described in detail below with reference to specific embodiments.
[0024] See also Figures 1 to 4 The embodiment of the present invention provides a rapid detection device for sulfate-reducing bacteria, which includes a detection bottle 1, a storage cylinder 3 and an impregnated carbon column 4. The detection bottle 1 is equipped with a detachable bottle cap 2, and the bottle cap 2 is fixedly equipped with a conduit 22. The detection bottle 1 replaces the sulfate-reducing bacteria detection bottle in the patent document with publication number CN216404362U, and the conduit 22 is used to communicate with the triangular flask.
[0025] Among them, a magnetic ring A11 is fixedly installed on the bottle mouth of the test bottle 1, and a magnetic ring B21 is fixedly installed inside the bottle cap 2, and the magnetic properties of the magnetic ring B21 are opposite to those of the magnetic ring A11. Since the bottle cap 2 is connected to the test bottle 1 by magnetic attraction, it is convenient to remove the bottle cap 2 and replace the impregnated carbon column 4.
[0026] In the embodiments of the present invention, please refer to Figures 1 to 4 The storage cylinder 3 is provided with an abutting assembly 5, which includes a sliding rod 51. The sliding rod 51 is inserted into the mounting cavity 3a opened in the storage cylinder 3 and is slidably connected to the mounting cavity 3a. A supporting base plate 52 for sealing the bottom opening of the storage cylinder 3 is installed at the bottom of the sliding rod 51. In this embodiment, the supporting base plate 52 is rotatably mounted on the bottom of the sliding rod 51, and a tension spring 53 is fixedly mounted on the top of the sliding rod 51, and the other end of the tension spring 53 is fixedly connected to the inner top wall of the mounting cavity 3a.
[0027] Need to explain: before installing the impregnated carbon column 4, take off the bottle cap 2 and hold the storage cylinder 3, then deflect the supporting disc 52 so that the bottom of the storage cylinder 3 is open to the structure, then insert the impregnated carbon column 4 into the storage cylinder 3, then pull down the supporting disc 52, so that the sliding rod 51 slides down along the installation cavity 3a and stretches the tension spring 53, until the supporting disc 52 is below the bottom of the impregnated carbon column 4, then rotate the supporting disc 52 so that the supporting disc 52 corresponds to the bottom of the impregnated carbon column 4, then loosen the supporting disc 52, so that the sliding rod 51 is combined with the impregnated carbon column 4 under the action of the elastic force of the tension spring 53, thus effectively fixing the impregnated carbon column 4;
[0028] And when replacing the impregnated carbon column 4, deflect the supporting disc 52 so that the bottom of the storage cylinder 3 is open to the structure, then pour out the impregnated carbon column 4 from the storage cylinder 3, which is simple and convenient to operate.
[0029] Further, the diameter of the edge of the cylinder opening at the bottom of the storage cylinder 3 is greater than the inner diameter of the installation cavity 2a, so as to avoid the storage cylinder 3 from slipping out of the installation cavity 2a.
[0030] In the embodiment of the utility model, please refer to Figures 1 to 4 , the bottle cap 2 is provided with an installation cavity 2a, and the installation cavity 2a is provided with an elastically connected storage cylinder 3, the storage cylinder 3 is provided with a spring 6, one end of the spring 6 is fixedly connected with the edge of the upper cylinder surface of the storage cylinder 3, and the other end of the spring 6 is fixedly connected with the upper surface of the bottle cap 2, the outer cylinder wall of the storage cylinder 3 is provided with an elastic telescopic sleeve 7, the elastic telescopic sleeve 7 is sleeved on the storage cylinder 3 and covers the spring 6, one end of the elastic telescopic sleeve 7 is fixedly connected with the edge of the upper cylinder surface of the storage cylinder 3, and the other end of the elastic telescopic sleeve 7 is fixedly connected with the upper surface of the bottle cap 2, the storage cylinder 3 stores an impregnated carbon column 4, and the cylinder wall of the storage cylinder 3 is provided with a plurality of annularly distributed through grooves 3b;
[0031] The bottle cap 2 is also provided with a positioning assembly 8 for positioning the sliding of the storage cylinder 3 into the detection bottle 1 to detect the depth, and the positioning assembly 8 comprises a guide rod 81, the guide rod 81 is fixedly installed on the bottle cap 2, a sleeve ring 82 is sleeved on the guide rod 81 in a sliding connection mode, the sleeve ring 82 is fixedly connected with the cylinder top of the storage cylinder 3, and a fastening screw rod 83 is inserted on the sleeve ring 82 in a threaded connection mode.
[0032] Need to explain: when the gas produced by fermentation in the triangular bottle enters the detection bottle 1, the gas reacts with the lead acetate reagent in the detection bottle 1 to generate black precipitate, accompanied by a rotten egg smell, which indicates that there is sulfate reducing bacteria in the fermentation bottle;
[0033] In the initial state, the edge of the bottom of the storage tube 3 abuts against the bottle cap 2 under the action of the spring 6, and the bottom of the storage tube 3 is sealed by the supporting plate 52. Therefore, the impregnated carbon column 4 in the storage tube 3 does not adsorb (or adsorbs very little) H2S. In addition, the elastic telescopic sleeve 7 is also provided on the storage tube 3 to prevent the gas in the gap between the storage tube 3 and the installation cavity 2a from escaping into the air.
[0034] When the experiment is over, the storage tube 3 is pressed down to drive the storage tube 3 to slide into the detection bottle 1. At this time, the spring 6 is compressed. After sliding to a suitable height (higher than the liquid level of the lead acetate reagent), the fastening screw 83 is rotated to fix the storage tube 3 on the guide rod 81, thereby locking the depth of the storage tube 3 sliding into the detection bottle 1. At this time, the elastic telescopic sleeve 7 is contracted. Since the storage tube 3 has a through groove 3b on its periphery, the unreacted H2S can be quickly absorbed by the impregnated carbon column 4 in the storage tube 3, avoiding the hidden danger of H2S overflow after the detection bottle 1 is opened.
[0035] In this embodiment, the impregnation carbon column 4 can be selected according to the needs. For example, the impregnating agent in the impregnated carbon column 4 can be any one of potassium iodide (KI), zinc oxide (ZnO) or potassium hydroxide (KOH).
[0036] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the description and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.
Claims
1. A rapid detection device for sulfate-reducing bacteria, comprising a detection bottle (1), a detachable bottle cap (2) mounted on the detection bottle (1), and a conduit (22) fixedly mounted on the bottle cap (2), characterized in that: The bottle cap (2) is provided with a mounting cavity (2a), and an elastically connected storage cylinder (3) is inserted into the mounting cavity (2a), and an impregnated carbon column (4) is stored in the storage cylinder (3), a cylinder wall of the storage cylinder (3) is provided with a plurality of annularly distributed through grooves (3b), and an elastic telescopic sleeve (7) is installed on the outer cylinder wall of the storage cylinder (3); The bottle cap (2) is also provided with a positioning assembly (8) for positioning the depth of the storage cylinder (3) sliding into the detection bottle (1).
2. The rapid detection device for sulfate-reducing bacteria according to claim 1, characterized in that: The positioning assembly (8) comprises a guide rod (81), the guide rod (81) being fixedly mounted on the bottle cap (2), and a slidingly connected collar (82) being sleeved on the guide rod (81), the collar (82) being fixedly connected to the top of the storage cylinder (3), and a threaded fastening screw (83) being inserted into the collar (82).
3. The rapid detection device for sulfate-reducing bacteria according to claim 1, characterized in that: A spring (6) is sleeved on the storage cylinder (3), one end of the spring (6) is fixedly connected to the edge of the upper surface of the storage cylinder (3), and the other end of the spring (6) is fixedly connected to the upper surface of the bottle cap (2).
4. The rapid detection device for sulfate-reducing bacteria according to claim 3, characterized in that: The elastic telescopic sleeve (7) is sleeved on the storage cylinder (3) and covers the spring (6); one end of the elastic telescopic sleeve (7) is fixedly connected to the edge of the upper surface of the storage cylinder (3), and the other end of the elastic telescopic sleeve (7) is fixedly connected to the upper surface of the bottle cap (2).
5. The rapid detection device for sulfate-reducing bacteria according to claim 1, characterized in that: The storage cylinder (3) is provided with an abutting assembly (5), the abutting assembly (5) comprising a sliding rod (51), the sliding rod (51) being inserted into a mounting cavity (3a) provided in the storage cylinder (3) and being slidably connected to the mounting cavity (3a), and a supporting plate (52) for sealing the bottom opening of the storage cylinder (3) being provided at the bottom of the sliding rod (51), a tension spring (53) being fixedly provided at the top of the sliding rod (51), and the other end of the tension spring (53) being fixedly connected to the inner top wall of the mounting cavity (3a).
6. The rapid detection device for sulfate-reducing bacteria according to claim 5, characterized in that: The diameter of the edge of the barrel opening at the bottom of the storage barrel (3) is larger than the inner diameter of the installation cavity (2a).
7. The rapid detection device for sulfate-reducing bacteria according to claim 1, characterized in that: A magnetic ring A (11) is fixedly mounted on the mouth of the detection bottle (1), and a magnetic ring B (21) is fixedly mounted inside the bottle cap (2), wherein the magnetic properties of the magnetic ring B (21) are opposite to those of the magnetic ring A (11).
Citation Information
Patent Citations
Sulfate reducing bacteria culture and detection device for laboratory
CN216404362U