Stem cell in-vitro dynamic scale amplification device
By designing a dynamic in vitro scale expansion device for stem cells and using a multi-layer culture plate and a peristaltic pump liquid supply system, the problems of uneven cell distribution and sterility were solved, and efficient and low-cost stem cell culture was achieved, which is suitable for large-scale commercial applications.
Patent Information
- Application Number
- CN202422110426.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-29
- Publication Date
- 2025-10-10
- Estimated Expiration
- 2034-08-29
AI Technical Summary
Existing stem cell culture devices have problems with sterility and consistency caused by uneven cell distribution and open culture. They are also inefficient and costly, unable to meet GMP requirements, thus limiting the development of the stem cell industry.
An in vitro dynamic scale expansion device for stem cells was designed, which uses multi-layer culture plates, a peristaltic pump liquid supply system and a closed sterile environment to ensure uniform cell distribution and gas exchange, thereby achieving efficient and low-cost cell culture.
It achieves uniform distribution of cells in a closed sterile environment, improves culture efficiency, meets GMP requirements, reduces costs, and adapts to large-scale commercial application needs.
Smart Images
Figure CN223422690U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of stem cell culture, in particular to a dynamic scale culture device for stem cells. Background Art
[0002] A stem cell in vitro dynamic scale expansion device is a high-precision device designed specifically to maintain healthy stem cell growth. It typically features advanced temperature control systems, gas concentration control systems, and culture medium supply control systems to maintain a constant temperature of around 37°C, close to human body temperature. The gas control system automatically adjusts the concentrations of carbon dioxide and oxygen to ensure optimal cell growth.
[0003] In the prior art, some cell culture devices, when in use, will have unfixed culture plates that are unevenly distributed during the culture process. This will lead to uneven cell distribution and affect cell growth and differentiation. Some cells will concentrate in certain areas, while no cells will grow in other areas, resulting in inconsistent culture results. At the same time, the culture process is almost always open and requires constant manual fluid replacement, making it impossible to ensure the sterility of the culture process and the consistency of the culture results. In addition, existing stem cell culture devices are almost all used in scientific research. The culture volume per unit space is extremely small, the efficiency is extremely low, and it does not meet GMP requirements. The high cost cannot meet the market demand for quantity and quality, thus greatly hindering the development of stem cell and product-related industries. To this end, a stem cell in vitro dynamic scale expansion device is proposed to solve the above problems. Utility Model Content
[0004] In order to make up for the above shortcomings, the utility model provides a stem cell in vitro dynamic scale expansion device, which aims to transform the small-batch, low-efficiency, and high-cost cultivation of stem cells, which has only remained in the field of scientific research applications, into large-scale, high-efficiency, and low-cost commercial applications, thereby promoting the development of industries related to stem cells and their products.
[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions:
[0006] A device for dynamic in vitro scale expansion of stem cells comprises a box body, the front end of the box body is rotatably connected to a box door, the front top of the box door is fixedly connected to a control screen, the rear bottom of the box body is fixedly connected to two fans, the inner bottom end of the box body is fixedly connected to a waste liquid collection box, a culture environment control system is installed inside the box body, which includes a gas environment control module and a temperature environment control module, a plurality of placement plates are fixedly installed on the left and right sides of the inner wall of the box body, a material placement cavity is formed between the placement plates, and multiple layers of culture plates are slidably connected in the material placement cavity, a nutrient solution storage box and a liquid supply device are also fixedly installed in the box body, the nutrient solution storage box is connected to the liquid supply device through a pipeline, the liquid supply device is connected to the multiple layers of culture plates through a pipeline, and the multiple layers of culture plates are also connected to the waste liquid collection box through a pipeline.
[0007] Furthermore, the multi-layer culture plate is composed of multiple cell culture plates stacked together, and the upper surface of the cell culture plate is fixedly provided with a culture fluid overflow area, a culture fluid inflow area and a cell growth area. The culture fluid overflow area and the culture fluid inflow area are arranged on both sides of the cell growth area, and the lower end surface of the culture fluid overflow area is fixedly provided with an overflow hole.
[0008] Furthermore, an upper sealing fixing groove is fixedly opened on the outer side of the upper end of the cell culture plate, and a lower buckling sealing groove is fixedly opened on the lower end surface of the cell culture plate. Multiple cell culture plates are buckled and connected to each other through the upper sealing fixing groove and the lower buckling sealing groove.
[0009] Furthermore, the cell culture plate is fastened to the adjacent upper or lower cell culture plate in a manner of fastening in opposite directions, i.e., the culture fluid inflow area of the upper cell culture plate corresponds to the culture fluid overflow area of the lower culture plate in upper and lower positions, and the culture fluid overflow area of the upper cell culture plate corresponds to the culture fluid inflow area of the lower culture plate in upper and lower positions.
[0010] Furthermore, the liquid supply device comprises a plurality of peristaltic pumps, the input ends of the plurality of peristaltic pumps are connected to the nutrient solution storage tank via a pipeline, and the output ends are connected to the liquid inlet end of the multi-layer culture plate via a pipeline.
[0011] Furthermore, a barrier protrusion is provided between the culture fluid inflow area and the cell growth area. The barrier protrusion is a right triangle, and a buffer protrusion is fixedly provided at the connecting end of the barrier protrusion and the cell growth area.
[0012] The utility model has the following beneficial effects:
[0013] The utility model realizes the stability of the culture environment, the dynamics of the culture fluid, and the initiative of gas exchange, so that the cells are evenly distributed throughout the culture process and obtain better gas exchange, nutrient supplementation, and timely discharge of metabolic waste. In particular, the entire culture process is carried out in a closed and sterile environment and can be quickly adapted to the culture process based on GMP requirements. This has very important economic value for obtaining a large number of healthy and sterile stem cells and supernatants for subsequent development of related cell products, and is particularly crucial for effectively obtaining a large number of healthy stem cells in a short period of time.
[0014] By using specialized, flexible multi-layer culture plates, the number of cells cultured within a limited space can be multiplied and costs can be reduced, which is particularly important for applications requiring large numbers of cells or highly concentrated supernatants. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 It is a three-dimensional schematic diagram of the utility model;
[0016] Figure 2 This is a schematic structural diagram of the fan of the present invention;
[0017] Figure 3 This is a schematic diagram of the main internal structure of the utility model;
[0018] Figure 4 This is a schematic diagram of the top view of a single cell culture plate of the present invention;
[0019] Figure 5 This is a schematic diagram of the main cross-sectional structure of a single cell culture plate of the present invention.
[0020] Legend:
[0021] 1. Chamber; 2. Chamber door; 3. Control panel; 4. Fan; 5. Waste liquid collection box; 6. Placement plate; 7. Multi-layer culture plate; 8. Nutrient solution storage tank; 9. Liquid supply device; 10. Cell culture plate; 11. Culture solution overflow area; 12. Culture solution inflow area; 13. Cell growth area; 14. Overflow hole; 15. Upper sealing fixing groove; 16. Lower buckle sealing groove; 17. Barrier protrusion; 18. Buffer protrusion. DETAILED DESCRIPTION
[0022] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0023] See also Figure 1-5 As shown, a stem cell in vitro dynamic scale expansion device includes a box body 1, the front end of the box body 1 is rotatably connected to a box door 2, the front end top of the box door 2 is fixedly connected to a control screen 3, the rear end bottom of the box body 1 is fixedly connected to two fans 4, the inner bottom end of the box body 1 is fixedly connected to a waste liquid collection box 5, a culture environment control system is installed inside the box body 1, which includes a gas environment control module and a temperature environment control module, a plurality of placement plates 6 are fixedly installed on the left and right sides of the inner wall of the box body 1, a material placement cavity is formed between the placement plates 6, and a multi-layer culture plate 7 is slidably connected in the material placement cavity, a nutrient solution storage tank 8 and a liquid supply device 9 are also fixedly installed in the box body 1, the nutrient solution storage tank 8 is connected to the liquid supply device 9 through a pipeline, the liquid supply device 9 is connected to the multi-layer culture plate 7 through a pipeline, and the multi-layer culture plate 7 is also connected to the waste liquid collection box 5 through a pipeline.
[0024] The multi-layer culture plate 7 is composed of multiple cell culture plates 10 stacked together, and the upper surface of the cell culture plate 10 is fixedly provided with a culture fluid overflow area 11, a culture fluid inflow area 12 and a cell growth area 13. The culture fluid overflow area 11 and the culture fluid inflow area 12 are opened on both sides of the cell growth area 13, and the lower end surface of the culture fluid overflow area 11 is fixedly provided with an overflow hole 14.
[0025] An upper sealing fixing groove 15 is fixedly opened on the outer side of the upper end of the cell culture plate 10, and a lower buckling sealing groove 16 is fixedly opened on the lower end surface of the cell culture plate 10. Multiple cell culture plates 10 are buckled and connected to each other through the upper sealing fixing groove 15 and the lower buckling sealing groove 16.
[0026] The cell culture plate 10 is fastened to the adjacent upper or lower cell culture plate 10 in opposite directions, i.e., the culture fluid inflow area 12 of the upper cell culture plate 10 corresponds to the culture fluid overflow area 11 of the lower culture plate in upper and lower positions, and the culture fluid overflow area 11 of the upper cell culture plate 10 corresponds to the culture fluid inflow area 12 of the lower culture plate in upper and lower positions.
[0027] The liquid supply device 9 comprises a plurality of peristaltic pumps, the input ends of the plurality of peristaltic pumps are connected to the nutrient solution storage tank 8 via a pipeline, and the output ends are connected to the liquid inlet end of the multi-layer culture plate 7 via a pipeline.
[0028] A blocking protrusion 17 is provided between the culture fluid inflow area 12 and the cell growth area 13 . The blocking protrusion 17 is a right triangle, and a buffering protrusion 18 is fixedly provided at the connection end between the blocking protrusion 17 and the cell growth area 13 .
[0029] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for part of the technical features of the equivalent replacement, the spirit and principles of the present application, made any modification, equivalent replacement, improvement, etc., should be included within the scope of the present application.
Claims
1. A stem cell in vitro dynamic scale expansion device, comprising a housing (1), characterized in that: The front end of the box body (1) is rotatably connected to a box door (2), the front top of the box door (2) is fixedly connected to a control screen (3), the rear bottom of the box body (1) is fixedly connected to two fans (4), the inner bottom of the box body (1) is fixedly connected to a waste liquid collection box (5), a culture environment control system is installed inside the box body (1), which includes a gas environment control module and a temperature environment control module, a plurality of placement plates (6) are fixedly installed on the left and right sides of the inner wall of the box body (1), a material placement cavity is formed between the placement plates (6), and a multi-layer culture plate (7) is slidably connected in the material placement cavity, a nutrient solution storage box (8) and a liquid supply device (9) are also fixedly installed in the box body (1), the nutrient solution storage box (8) and the liquid supply device (9) are connected through a pipeline, the liquid supply device (9) and the multi-layer culture plate (7) are connected through a pipeline, and the multi-layer culture plate (7) is also connected to the waste liquid collection box (5) through a pipeline.
2. The device for in vitro dynamic scale expansion of stem cells according to claim 1, characterized in that: The multi-layer culture plate (7) is composed of a plurality of stacked cell culture plates (10). The upper surface of the cell culture plate (10) is fixedly provided with a culture fluid overflow area (11), a culture fluid inflow area (12) and a cell growth area (13). The culture fluid overflow area (11) and the culture fluid inflow area (12) are provided on both sides of the cell growth area (13). The lower end surface of the culture fluid overflow area (11) is fixedly provided with an overflow hole (14).
3. The device for in vitro dynamic scale expansion of stem cells according to claim 2, characterized in that: An upper sealing fixing buckle groove (15) is fixedly provided on the outer side of the upper end of the cell culture plate (10), and a lower buckling sealing groove (16) is fixedly provided on the lower end surface of the cell culture plate (10). A plurality of cell culture plates (10) are buckled and connected to each other via the upper sealing fixing buckle groove (15) and the lower buckling sealing groove (16).
4. The device for in vitro dynamic scale expansion of stem cells according to claim 3, characterized in that: The cell culture plate (10) is fastened to the adjacent upper or lower cell culture plate (10) in a manner of fastening in opposite directions, i.e., the culture fluid inflow area (12) of the upper cell culture plate (10) corresponds to the culture fluid overflow area (11) of the lower culture plate in upper and lower positions, and the culture fluid overflow area (11) of the upper cell culture plate (10) corresponds to the culture fluid inflow area (12) of the lower culture plate in upper and lower positions.
5. The in vitro dynamic scale expansion device for stem cells according to claim 1, characterized in that: The liquid supply device (9) comprises a plurality of peristaltic pumps, the input ends of the plurality of peristaltic pumps are connected to the nutrient solution storage tank (8) via a pipeline, and the output ends are connected to the liquid inlet end of the multi-layer culture plate (7) via a pipeline.
6. The in vitro dynamic scale expansion device for stem cells according to claim 2, characterized in that: A blocking protrusion (17) is provided between the culture fluid inflow area (12) and the cell growth area (13). The blocking protrusion (17) is a right triangle, and a buffering protrusion (18) is fixedly provided at the connection end between the blocking protrusion (17) and the cell growth area (13).