Cell culture reactor
The gear system driven by the servo motor makes the culture fluid inside the cell culture reactor shake, which solves the problem of uneven consumption of culture fluid limiting cell proliferation, achieves sufficient mixing of cells and culture fluid and accelerates cell proliferation.
Patent Information
- Application Number
- CN202422753767.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-10-10
- Estimated Expiration
- 2034-11-12
AI Technical Summary
In existing cell culture reactors, uneven consumption of culture fluid limits cell proliferation, and the reactor needs to be shaken to accelerate the mixing of cells and culture fluid.
A cell culture reactor was designed. A servo motor drives a gear system to move the moving rod and the fixing assembly, causing the culture fluid inside the reactor to shake, thereby achieving sufficient mixing of cells and the culture fluid.
By shaking the culture medium, the cell proliferation process is accelerated and the cell density is increased.
Smart Images
Figure CN223422695U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture reactor. Background Art
[0002] Cell culture reactors, especially cell culture bioreactors, are key equipment for cell culture and biological reactions under controlled conditions. They provide an ideal environment to support cell growth, proliferation and production of desired biological products. These reactors can precisely control environmental conditions such as temperature, oxygen and carbon dioxide concentrations, and pH value to meet the needs of cell growth and metabolism.
[0003] However, existing cell culture reactors are mainly used for the culture of suspended cells and are placed statically during the culture process. However, since the culture fluid is in a static state during cell growth, the culture fluid will be consumed unevenly, thereby limiting the proliferation of cells. In order to obtain a higher cell density, the reactor needs to be shaken to fully mix the cells and the culture fluid to accelerate cell proliferation. A cell culture reactor is now proposed to solve the above problems. Utility Model Content
[0004] The utility model aims at the technical problems existing in the prior art and provides a cell culture reactor, which solves the problem that the culture fluid is consumed unevenly and thus restricts cell proliferation.
[0005] The technical solution of the utility model for solving the above technical problems is as follows: A cell culture reactor comprises a reaction table, the top of the reaction table is fixedly connected to a servo motor, the output end of the servo motor is fixedly connected to a drive shaft, the middle part of the drive shaft is fixedly connected to a first spur gear, both ends of the drive shaft are rotatably connected to a fixed seat, one side of the fixed seat is meshed with a driven component, one side of the driven component is movably connected to an elastic component, the middle part of the elastic component is fixedly connected to a reaction plate, and the top of the reaction plate is fixedly connected to a fixing component.
[0006] Preferably, the driven assembly includes a second spur gear, a middle portion of the second spur gear is fixedly connected to a driven shaft, and both ends of the driven shaft are fixedly connected to driven plates.
[0007] Preferably, one side of the second spur gear is meshed with the first spur gear, both ends of the driven shaft are rotatably connected to the reaction table, and one side of the driven plate is movably connected to the reaction table.
[0008] Preferably, the elastic component includes a moving rod, both ends of the moving rod are fixedly sleeved with return springs, both sides of the top of the moving rod are fixedly connected to limit blocks, and one end of the return spring is fixedly connected to the fixed rod.
[0009] Preferably, the outer wall of the movable rod is fixedly connected to the reaction plate, the limit block is a T-shaped structure, the outer wall of the limit block is movably engaged with the reaction table, and both ends of the fixed rod are fixedly connected to the reaction table.
[0010] Preferably, the fixing assembly includes a hinged seat, both ends of which are fixedly connected to hinged rods, the middle part of one hinged rod is hinged to a first clamping plate, the middle part of the other hinged rod is hinged to a second clamping plate, and the top of the first clamping plate is linearly threaded with a fixing nut.
[0011] Preferably, the bottom of the hinged seat is fixedly connected to the reaction plate, one side of the first clamping plate is movably connected to the second clamping plate, and the fixing nut is threadedly connected to the second clamping plate.
[0012] The beneficial effect of the utility model is that the utility model is provided with a first spur gear, a driven assembly, an elastic assembly, a reaction plate, a clamping assembly and the like, wherein the servo motor drives the driving shaft to rotate, the driving shaft drives the first spur gear to rotate, the first spur gear and the second spur gear are meshed and the first spur gear drives the second spur gear to rotate, the second spur gear drives the driven shaft to rotate, the driven shaft drives the driven plate to rotate, so that the arc surface of the driven plate pushes the moving rod, the moving rod drives the limit block to slide along both sides of the reaction table, the moving rod pulls the reset spring to stretch and extend, when the arc surface of the driven plate rotates to one circle, the straight surface of the driven plate tends to be horizontal, the moving rod does not abut against the arc surface of the driven plate, the rebound force of the reset spring pulls the moving rod to reset the moving rod, and the continuous rotation of the driven plate enables the moving rod to drive the clamping assembly to move repeatedly through the reaction plate, causing the clamping assembly to shake, thereby causing the culture medium in the reactor to shake, so that the culture medium and cells are fully mixed, thereby accelerating the proliferation of cells. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 It is a schematic diagram of the overall positional relationship structure of the utility model;
[0014] Figure 2 It is a schematic diagram of the cross-sectional structure of the entire utility model;
[0015] Figure 3 This is a schematic diagram of the positional relationship structure of the clamping assembly of the present invention;
[0016] Figure 4 This is a schematic diagram of the positional relationship structure of the elastic component of the utility model;
[0017] Figure 5 This is a schematic diagram of the positional relationship structure of the driven component of the utility model.
[0018] In the accompanying drawings, the components represented by the reference numerals are as follows:
[0019] 1. Reaction table, 2. Servo motor, 3. Drive shaft, 4. First spur gear, 5. Fixed seat, 6. Driven assembly, 61. Second spur gear, 62. Driven shaft, 63. Driven plate, 7. Elastic assembly, 71. Moving rod, 72. Return spring, 73. Limit block, 74. Fixed rod, 8. Reaction plate, 9. Fixing assembly, 91. Articulated seat, 92. Articulated rod, 93. First clamping plate, 94. Second clamping plate, 95. Fixing nut. DETAILED DESCRIPTION
[0020] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without making creative efforts are within the scope of protection of this application.
[0021] In the description of this application, the terms "first" and "second" are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly specify the number of the technical features indicated. Therefore, a feature specified as "first" or "second" may explicitly or implicitly include one or more of the described features. In the description of this application, "plurality" means two or more, unless otherwise specifically specified.
[0022] In the description of this application, the term "for example" is used to mean "used as an example, illustration or explanation". Any embodiment described as "for example" in this application is not necessarily to be construed as being more preferred or advantageous than other embodiments. In order to enable any person skilled in the art to implement and use the present invention, the following description is given. In the following description, details are listed for the purpose of explanation. It should be understood that a person of ordinary skill in the art can recognize that the present invention can be implemented without using these specific details. In other examples, well-known structures and processes will not be elaborated in detail to avoid obscuring the description of the present invention with unnecessary details. Therefore, the present invention is not intended to be limited to the embodiments shown, but is consistent with the widest scope consistent with the principles and features disclosed in this application.
[0023] Example 1
[0024] like Figures 1 to 5As shown, the utility model provides a cell culture reactor, including a reaction table 1, a servo motor 2 is fixedly connected to the top of the reaction table 1, the output end of the servo motor 2 is fixedly connected to a drive shaft 3, the middle of the drive shaft 3 is fixedly connected to a first spur gear 4, both ends of the drive shaft 3 are rotatably connected to a fixed seat 5, one side of the fixed seat 5 is meshed with a driven component 6, one side of the driven component 6 is movably connected to an elastic component 7, the middle of the elastic component 7 is fixedly connected to a reaction plate 8, and the top of the reaction plate 8 is fixedly connected to a fixing component 9;
[0025] The above scheme is adopted: by providing a first spur gear 4, a driven component 6, an elastic component 7, a reaction plate 8, a clamping component 9 and other structures, the servo motor 2 drives the driving shaft 3 to rotate, the driving shaft 3 drives the first spur gear 4 to rotate, the first spur gear 4 is meshed with the second spur gear 61 and makes the first spur gear 4 drive the second spur gear 61 to rotate, the second spur gear 61 drives the driven shaft 62 to rotate, the driven shaft 62 drives the driven plate 63 to rotate, so that the arc surface of the driven plate 63 pushes the moving rod 71, and the moving rod 71 drives the limit block 73 to slide along both sides of the reaction table 1, and the moving plate 63 is rotated. The movable rod 71 pulls the return spring 72, causing the return spring 72 to stretch and extend. When the arc surface of the driven plate 63 rotates one circle, the straight surface of the driven plate 63 tends to be horizontal, and the movable rod 71 does not abut the arc surface of the driven plate 63. The movable rod 71 is pulled by the rebound force of the return spring 72, so that the movable rod 71 is reset. Through the continuous rotation of the driven plate 63, the movable rod 71 can drive the fixing assembly 9 to move repeatedly through the reaction plate 8, causing the fixing assembly 9 to shake, thereby causing the culture medium in the reactor to shake, so that the culture medium and cells are fully mixed, and the cell proliferation is accelerated.
[0026] Example 2
[0027] like Figures 2 to 5 As shown, the driven assembly 6 includes a second spur gear 61, a driven shaft 62 is fixedly connected to the middle portion of the second spur gear 61, and driven plates 63 are fixedly connected to both ends of the driven shaft 62;
[0028] One side of the second spur gear 61 is meshed with the first spur gear 4 , both ends of the driven shaft 62 are rotatably connected to the reaction table 1 , and one side of the driven plate 63 is movably connected to the reaction table 1 ;
[0029] With the above scheme: by rotating the servo motor 2 to drive the driving shaft 3, the driving shaft 3 drives the first spur gear 4 to rotate, the first spur gear 4 meshes with the second spur gear 61 and drives the second spur gear 61 to rotate, the second spur gear 61 drives the driven shaft 62 to rotate, the driven shaft 62 drives the driven plate 63 to rotate, so that the arc surface of the driven plate 63 pushes the moving rod 71, the reaction table 1 supports and fixes the driven shaft 62, the second spur gear 61 supports the second spur gear 61, so that the second spur gear 61 can correctly mesh with the first spur gear 4, the reaction table 1 supports and fixes the servo motor 2, the reaction table 1 limits and fixes the fixed seat 5, the fixed seat 5 limits and supports the driving shaft 3, to prevent the servo motor 2 from moving during the rotation of the driving shaft 3.
[0030] Example 3
[0031] As Figures 2 to 5 shown, the elastic assembly 7 includes a moving rod 71, both ends of the moving rod 71 are fixedly sleeved with a reset spring 72, both sides of the top of the moving rod 71 are fixedly connected with a limiting block 73, one end of the reset spring 72 is fixedly connected with a fixed rod 74;
[0032] The outer wall of the moving rod 71 is fixedly connected with the reaction plate 8, the limiting block 73 is a T-shaped structure, the outer wall of the limiting block 73 is movably clamped with the reaction table 1, and the two ends of the fixed rod 74 are fixedly connected with the reaction table 1;
[0033] With the above scheme: during the movement of the moving rod 71 pushed by the driven plate 63, the moving rod 71 drives the limiting block 73 to slide along the two sides of the reaction table 1, the moving rod 71 pulls the reset spring 72, so that the reset spring 72 is stretched and elongated, when the arc surface of the driven plate 63 turns a circle, the straight surface of the driven plate 63 tends to be horizontal, the moving rod 71 does not abut against the arc surface of the driven plate 63, the moving rod 71 is pulled by the rebounding force of the reset spring 72, so that the moving rod 71 is reset, through the continuous rotation of the driven plate 63, the moving rod 71 can drive the clamping assembly 9 to move repeatedly through the reaction plate 8, so that the cells in the reactor and the culture solution are shaken, the culture solution and the cells are fully mixed, and the proliferation of the cells is accelerated;
[0034] The moving rod 71 is limited and supported by the reaction table 1. Transverse grooves that are adapted to the size of the moving rod 71 are provided on both sides of the reaction table 1. A T-shaped slot that is adapted to the size of the limit block 73 is provided on the top of the transverse slot. When the moving rod 71 drives the limit block 73 to slide along the two sides of the reaction table 1, the moving rod 71 moves along the transverse slot. When the moving rod 71 pulls the return spring 72, the limit block 73 moves along the T-shaped slot through the movement of the moving rod 71. The limit block 73 is limited by the T-shaped slot, and the moving rod 71 is limited by the transverse slot. The reaction table 1 limits the fixed rod 74, and the fixed rod 74 fixes one end of the return spring 72, effectively preventing the return spring 72 from falling off when stretched. The reaction plate 8 has a T-shaped structure.
[0035] Example 4
[0036] like Figures 2 to 5 As shown, the clamping assembly 9 includes an articulated seat 91, and both ends of the articulated seat 91 are fixedly connected to articulated rods 92. A first clamping plate 93 is hingedly connected to the middle of one articulated rod 92, and a second clamping plate 94 is hingedly connected to the middle of the other articulated rod 92. The top of the first clamping plate 93 is linearly threadedly connected to a fixing nut 95;
[0037] The bottom of the hinged seat 91 is fixedly connected to the reaction plate 8, one side of the first clamping plate 93 is movably connected to the second clamping plate 94, and the fixing nut 95 is threadedly connected to the second clamping plate 94;
[0038] The above scheme is adopted: by placing the reactor inside the first clamping plate 93, and flipping the second clamping plate 94 with the hinge rod 92 as the center of the circle, so that the threaded holes at the top of the first clamping plate 93 and the second clamping plate 94 are aligned, and the fixing nut 95 is inserted into the threaded hole so that the fixing nut 95 connects and fixes the first clamping plate 93 and the second clamping plate 94, and the reactor is limited and fixed inside the clamping assembly 9, and the hinge seat 91 is supported and fixed by the reaction plate 8, and the hinge seat 91 fixes the two hinged rods 92, and the two hinged rods 92 are hinged to the bottom ends of the first clamping plate 93 and the second clamping plate 94 respectively. By hinged connection, the first clamping plate 93 and the second clamping plate 94 can be flipped with the hinge rod 92 as the center of the circle respectively, and the first clamping plate 93 and the second clamping plate 94 are connected and fixed by the fixing nut 95, so that the reactor is limited and fixed inside the clamping assembly 9, and the first clamping plate 93 and the second clamping plate 94 are both semicircular structures, and the first clamping plate 93 and the second clamping plate 94 can form a complete ring after being clamped.
[0039] The working principle and use process of this utility model:
[0040] First, place the reactor inside the first clamping plate 93, flip the second clamping plate 94 with the hinge rod 92 as the center, so that the threaded holes on the top of the first clamping plate 93 and the second clamping plate 94 are aligned, and the fixing nut 95 is inserted into the threaded hole so that the fixing nut 95 connects and fixes the first clamping plate 93 and the second clamping plate 94. At this time, the reactor is limited and fixed inside the clamping assembly 9, and the servo motor 2 drives the drive shaft 3 to rotate, and the drive shaft 3 drives the first spur gear 4 to rotate. The first spur gear 4 is meshed with the second spur gear 61, so that the first spur gear 4 drives the second spur gear 61 to rotate. At this time, the second spur gear 61 drives the driven shaft 62 to rotate, and the driven shaft 62 drives the driven plate 63 to rotate, so that the arc surface of the driven plate 63 pushes the moving rod 71;
[0041] When the movable rod 71 is moved by the push of the driven plate 63, the movable rod 71 drives the limit block 73 to slide along both sides of the reaction table 1. At this time, the movable rod 71 pulls the return spring 72, causing the return spring 72 to stretch and extend. When the arc surface of the driven plate 63 rotates to one circle, the straight surface of the driven plate 63 tends to be horizontal. At this time, the movable rod 71 does not abut the arc surface of the driven plate 63, and the movable rod 71 is pulled by the rebound force of the return spring 72, so that the movable rod 71 is reset. Through the continuous rotation of the driven plate 63, the movable rod 71 can drive the fixing assembly 9 to move repeatedly through the reaction plate 8, thereby causing the cells and culture medium inside the reactor to shake, so that the culture medium and cells are fully mixed, accelerating the proliferation of cells, and completing the operation.
[0042] It should be noted that, in the above embodiments, the description of each embodiment has its own focus. For parts that are not described in detail in a certain embodiment, reference can be made to the relevant description of other embodiments.
[0043] Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.
[0044] Obviously, those skilled in the art may make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations fall within the scope of the claims of the present invention and their equivalents, the present invention is intended to include such modifications and variations.
Claims
1. A cell culture reactor, comprising a reaction table (1), characterized in that: The top of the reaction table (1) is fixedly connected to a servo motor (2), the output end of the servo motor (2) is fixedly connected to a drive shaft (3), the middle of the drive shaft (3) is fixedly connected to a first straight gear (4), both ends of the drive shaft (3) are rotatably connected to a fixed seat (5), one side of the fixed seat (5) is meshed with a driven component (6), one side of the driven component (6) is movably connected to an elastic component (7), the middle of the elastic component (7) is fixedly connected to a reaction plate (8), and the top of the reaction plate (8) is fixedly connected to a clamping component (9).
2. The cell culture reactor according to claim 1, characterized in that: The driven assembly (6) comprises a second spur gear (61), a driven shaft (62) is fixedly connected to the middle of the second spur gear (61), and driven plates (63) are fixedly connected to both ends of the driven shaft (62).
3. The cell culture reactor according to claim 2, characterized in that: One side of the second spur gear (61) is meshed with the first spur gear (4), both ends of the driven shaft (62) are rotationally connected to the reaction table (1), and one side of the driven plate (63) is movably connected to the reaction table (1).
4. The cell culture reactor according to claim 1, wherein: The elastic assembly (7) comprises a moving rod (71), both ends of the moving rod (71) are fixedly sleeved with a return spring (72), both sides of the top of the moving rod (71) are fixedly connected to a limit block (73), and one end of the return spring (72) is fixedly connected to a fixed rod (74).
5. The cell culture reactor according to claim 4, characterized in that: The outer wall of the movable rod (71) is fixedly connected to the reaction plate (8), the limiting block (73) is a T-shaped structure, the outer wall of the limiting block (73) is movably connected to the reaction table (1), and both ends of the fixed rod (74) are fixedly connected to the reaction table (1).
6. The cell culture reactor according to claim 1, characterized in that: The clamping assembly (9) comprises an articulated seat (91), both ends of the articulated seat (91) are fixedly connected to articulated rods (92), a first clamping plate (93) is hingedly connected to the middle of one articulated rod (92), and a second clamping plate (94) is hingedly connected to the middle of the other articulated rod (92), and a fixing nut (95) is linearly threadedly connected to the top of the first clamping plate (93).
7. The cell culture reactor according to claim 6, characterized in that: The bottom of the hinged seat (91) is fixedly connected to the reaction plate (8), one side of the first clamping plate (93) is movably connected to the second clamping plate (94), and the fixing nut (95) is threadedly connected to the second clamping plate (94).