Kit convenient for placing sample bottle

Abstract

The utility model discloses a kit convenient for placing a sample bottle, which belongs to the technical field of kits, and comprises a kit for storing the sample bottle, a centrifugal separation device for performing centrifugal operation on the sample bottle is arranged in the kit, and the centrifugal separation device comprises a driving mechanism, an auxiliary mechanism and a rotating mechanism, the driving mechanism is arranged in the kit, the rotating mechanisms are arranged on the driving mechanism at equal intervals in a surrounding manner, and the auxiliary mechanism is arranged in the kit; the device can be used for placing and storing a plurality of sample bottles, in the storage process, centrifugal rotation operation can be performed on the sample bottles capable of being stored in the kit through starting of centrifugal separation equipment, exosomes stored in the sample bottles can be separated from impurities through centrifugation, the components of exosome samples are more uniform, and the purity of the exosome samples is improved. Therefore, more accurate analysis, detection and research of subsequent samples are improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of reagent box, specifically to a reagent box convenient for sample bottle placement. BACKGROUND

[0002] In recent years, exosome technology has become a hot spot for cancer detection and prognosis due to its non-invasiveness and rich biomarker content. The goal of this project is to embed these genes as potential markers into a model by combining saliva exosome extraction technology to achieve more accurate prognosis evaluation of laryngeal cancer. Advanced acoustofluidic technology (such as the Acoustofluidic platform) is used to extract exosomes from patient saliva. This technology can efficiently separate and extract exosomes, ensuring sample purity and biomarker stability. RNA / DNA is extracted from exosomes to detect the expression of TIMP1, MNX1, LCK, and MMP3, and high-precision molecular detection methods such as droplet digital PCR are used for analysis.

[0003] After sampling, the exosomes are placed in the required sample bottle for storage. In order to reflect the "timeliness", "accuracy" and convenience of carrying the sample exosome extraction, all completed sample bottles are placed in the required reagent box for storage, which can be carried conveniently and detected at any time. In order to ensure the accuracy of the exosome sample in the sample bottle, there is no reagent box that can store and centrifuge the required sample bottle. In order to analyze, detect and research the sample more accurately, it is very important to separate the impurities by centrifuging the sample. Therefore, the reagent box for placing the sample bottle is provided. UTILITY MODEL CONTENT

[0004] The utility model aims at the deficiency of prior art, provides a reagent box convenient for sample bottle placement to solve the above-mentioned technical problem.

[0005] The utility model embodiment adopts the following technical scheme: including the reagent box for storing sample bottle, the centrifugal separation equipment for sample bottle centrifugal operation is equipped in the reagent box, the centrifugal separation equipment includes drive mechanism, auxiliary mechanism and rotating mechanism, the drive mechanism sets up in the reagent box, the rotating mechanism is equipped with several, several rotating mechanism are all equidistantly around and set up on drive mechanism, the auxiliary mechanism sets up in the reagent box, and several rotating mechanism and auxiliary mechanism are respectively in the cooperation rotation state, the reagent box includes reagent chamber and reagent cover, the reagent cover sets up on reagent chamber and is openable setting.

[0006] Further, the driving mechanism comprises a driving motor, a driving wheel, a driven wheel and a belt, the driving motor is arranged in the reagent box, the driving wheel is arranged on the output end of the driving motor, the driven wheel is rotatably connected in the reagent box and located beside the driving wheel, and the belt is sleeved on the driving wheel and the driven wheel.

[0007] Further, the reagent box is provided with a control system for electrically connecting the driving motor, the driving motor is a direct current speed regulation motor, and the control system is provided with a control display screen, the control display screen is a touch display screen and can be used for controlling the driving motor to operate.

[0008] Further, the auxiliary mechanism comprises an auxiliary shaft and an auxiliary bevel gear, the middle part of the driven wheel is hollow, the auxiliary shaft is arranged on the reagent box and penetrates through the hollow position of the driven wheel, and the auxiliary bevel gear is arranged on the upper end of the auxiliary shaft.

[0009] Further, each of the rotating mechanisms comprises a rotating support, a rotating shaft and a rotating storage cylinder, the rotating support is arranged on the upper end of the driven wheel, the upper end of the rotating support is inclined, the rotating shaft is arranged on the upper end of the rotating support and faces the inner side, the rotating shaft is rotatably connected with the rotating support, a rotating gear matched with the auxiliary bevel gear is sleeved on the rotating shaft, and the rotating storage cylinder is arranged on the upper end of the rotating shaft.

[0010] Further, the rotating storage cylinder is internally provided with a storage groove, and the storage groove is arranged in a size matched with a conventional sample bottle.

[0011] Further, the inclination angle of the upper end of each rotating support is forty degrees.

[0012] The above at least one technical scheme adopted by the embodiment of the utility model can achieve the following beneficial effects:

[0013] The utility model discloses, can be used for the storage of multiple sample bottles, and the sample bottle can be taken at any time, and in the storage process, the sample bottle can be centrifuged by the centrifugal separation equipment, and the exosome in the sample bottle can be separated from the impurities, so that the composition of the exosome sample is more uniform, and the subsequent sample analysis, detection and research are more accurate. ACCURATE DESCRIPTION

[0014] The accompanying drawings, which are included to provide a further understanding of the utility model, form a part of the utility model, and the illustrative embodiments of the utility model and the description thereof are used to explain the utility model, and do not constitute improper limitation on the utility model.

[0015] Figure 1The utility model discloses a reagent cover opening state's structure schematic view.

[0016] Figure 2 The utility model discloses a three-dimensional structure schematic view.

[0017] Figure 3 The utility model discloses a centrifugal separation equipment's three-dimensional structure schematic view.

[0018] Figure 4 The utility model discloses a driving mechanism and rotating mechanism's three-dimensional structure schematic view.

[0019] Figure 5 For Figure 4 The utility model discloses a centrifugal separation equipment's three-dimensional structure schematic view.

[0020] The utility model discloses a centrifugal separation equipment's three-dimensional structure schematic view.

[0021] Reagent box 1, reagent chamber 11, reagent cover 12, centrifugal separation equipment 2, driving mechanism 21, driving motor 211, driving wheel 212, driven wheel 213, belt 214, control display screen 215, auxiliary mechanism 22, auxiliary shaft 221, auxiliary bevel gear 222, rotating mechanism 23, rotating support 231, rotating shaft 232, rotating storage cylinder 233, rotating gear 234, storage groove 235. Specific implementation

[0022] To make the utility model's purpose, technical scheme and advantage more clear, below will combine the utility model specific embodiment and corresponding drawing to the utility model technical scheme clear, complete description. Obviously, the described embodiment is only the utility model a part embodiment, is not all embodiment. Based on the embodiment in the utility model, all other embodiments that the ordinary skill in the art person obtains under no creative labor premise, all belong to the utility model protection's range.

[0023] The following will be combined with the drawings, and the technical scheme provided by each embodiment of the utility model will be described in detail.

[0024] The utility model embodiment provides a reagent box convenient for sample bottle placement, including the reagent box 1 that is used to store sample bottle, the centrifugal separation equipment 2 for sample bottle to carry out centrifugal operation is equipped in the reagent box 1, the centrifugal separation equipment 2 includes driving mechanism 21, auxiliary mechanism 22 and rotating mechanism 23, the driving mechanism 21 is arranged in the reagent box 1, the rotating mechanism 23 is equipped with several, several rotating mechanism 23 are equidistantly around setting on driving mechanism 21, the auxiliary mechanism 22 is arranged in the reagent box 1, and several rotating mechanism 23 and auxiliary mechanism 22 are in the cooperation rotating state respectively, the reagent box 1 includes reagent chamber 11 and reagent cover 12, the reagent cover 12 is arranged on reagent chamber 11 and is openable setting.

[0025] The device can be used for placing and storing a plurality of sample bottles, and during storage, the sample bottles capable of being stored in the kit 1 can be subjected to centrifugal rotation operation through the start of the centrifugal separation equipment 2, and through centrifugation, the exosomes stored in the sample bottles can be separated from the impurities, the composition of the exosome sample is more uniform, and therefore, subsequent sample analysis, detection and research are more accurate.

[0026] In a further preferred embodiment of the utility model, the driving mechanism 21 comprises a driving motor 211, a driving wheel 212, a driven wheel 213 and a belt 214, the driving motor 211 is arranged inside the kit 1, the driving wheel 212 is arranged on the output end of the driving motor 211, the driven wheel 213 is rotatably connected inside the kit 1 and located beside the driving wheel 212, the belt 214 is sleeved on the driving wheel 212 and the driven wheel 213, the kit 1 is provided with a control system for electrically connecting the driving motor 211, the driving motor 211 is a direct current speed regulation motor, the control system is provided with a control display screen 215, the control display screen 215 is a touch display screen and can be used to control the operation of the driving motor 211.

[0027] The driving motor 211 is a direct current speed regulation motor, and a control display screen 215 is arranged on the control system. Since saliva is a complex biological fluid of a reagent box convenient for sample bottle placement, containing cell fragments, protein aggregates, microorganisms and various components. The exosomes can be separated from these impurities by centrifugation. In the centrifugation process at a relatively low speed, such as 300-500g, centrifugation for 5-10 minutes, the cells and large cell fragments in the saliva can be removed first. These substances have a large specific gravity and will settle at the bottom of the centrifugal tube under the action of centrifugal force. Then, the speed is gradually increased, such as 10,000-20,000g, and centrifugation is performed for 30-60 minutes. Smaller impurity particles can be further removed, and the exosomes are enriched in the supernatant or precipitate after centrifugation, depending on the size and density characteristics of the exosomes. This helps to obtain a higher purity exosome sample for more accurate analysis and research in the subsequent process. Concentration of exosomes: In some cases, the concentration of exosomes in saliva may be low, which is not conducive to direct detection and research. Centrifugation can concentrate exosomes together. For example, when using ultracentrifugation at 100,000-200,000g, exosomes can be precipitated at the bottom of the centrifugal tube under the action of centrifugal force. Thus, the exosomes originally dispersed in a larger volume of saliva can be concentrated in a smaller volume range, facilitating subsequent extraction, analysis steps, such as proteomics, nucleic acid analysis, and other experiments, which can meet the sample concentration requirements of experiments. Therefore, the operator can set the rotation speed of the driving motor 211 through the control display screen 215 according to the type of sample, so as to adjust the rotation speed to a suitable speed according to different sample types and requirements.

[0028] In further preferable embodiments of the utility model, the auxiliary mechanism 22 includes an auxiliary shaft 221 and an auxiliary bevel gear 222, the middle part of the driven wheel 213 is hollow, the auxiliary shaft 221 is arranged on the reagent box 1 and the upper end passes through the hollow position of the driven wheel 213, the auxiliary bevel gear 222 is arranged on the upper end of the auxiliary shaft 221, and the plurality of rotating mechanisms 23 each include a rotating support 231, a rotating shaft 232 and a rotating storage cylinder 233, the rotating support 231 is arranged on the upper end of the driven wheel 213, the upper end of the rotating support 231 is arranged obliquely, the rotating shaft 232 is arranged on the upper end of the rotating support 231 and faces the inside, the rotating shaft 232 is rotationally connected with the rotating support 231, a rotating gear 234 that is adapted to and engaged with the auxiliary bevel gear 222 is sleeved on the rotating shaft 232, and the rotating storage cylinder 233 is arranged on the upper end of the rotating shaft 232.

[0029] When it is necessary to place and store the sample bottle, the reagent cover 12 is opened, the required sample bottle is placed into the corresponding rotating storage cylinder 233, and the reagent cover 12 is closed.

[0030] When centrifugation of the sample bottle inside the kit 1 is needed to achieve better subsequent detection, the driving motor 211 is started by operating the control display screen 215, and the driving motor 211 drives the driving wheel 212 on the output end of the driving motor 211 to rotate, the driving wheel 212 rotates to drive the driven wheel 213 inside the kit 1 through the cooperation of the belt 214, the driven wheel 213 rotates to drive the plurality of rotating supports 231 to rotate, the plurality of rotating supports 231 rotate to drive the corresponding rotating shafts 232 to rotate, the plurality of rotating shafts 232 rotate to drive the corresponding rotating gears 234 and the rotating storage barrels 233 to rotate around the auxiliary bevel gear 222, and because the plurality of rotating shafts 232 and the corresponding rotating supports 231 are rotationally connected, the plurality of rotating gears 234 rotate around the auxiliary bevel gear 222, and the plurality of rotating gears 234 also rotate around the auxiliary bevel gear 222 because the auxiliary bevel gear 222 is engaged, the plurality of rotating gears 234 rotate around the auxiliary bevel gear 222, and the plurality of rotating shafts 232 rotate around the auxiliary bevel gear 222, so that the sample bottles inside the plurality of rotating storage barrels 233 rotate around the auxiliary bevel gear 222, and the plurality of rotating storage barrels 233 also rotate, so as to achieve centrifugation of the stored sample bottles, and the exosomes inside the sample bottles are separated from the impurities by centrifugation, so that the composition of the exosome sample is more uniform, thereby improving the subsequent accurate analysis, detection and research of the sample.

[0031] In a further preferred embodiment of the present application, the rotating storage barrel 233 is provided with a storage groove 235, and the storage groove 235 is provided with a size suitable for the conventional sample bottle.

[0032] The rotating storage barrel 233 is provided with a storage groove 235, and the storage groove 235 is provided with a size suitable for the conventional sample bottle, so that the sample bottle can be conveniently placed.

[0033] In a further preferred embodiment of the present application, the inclination angle of the upper end of the plurality of rotating supports 231 is forty degrees.

[0034] The centrifugal rotation angle is generally between 20°-45°, the sample is placed at a certain angle with the rotation shaft during centrifugation, this design makes the centrifugal force have components in the vertical and horizontal directions, when the centrifuge starts, the sample will quickly gather to the outside of the centrifuge tube bottom. The advantage is that the centrifugal speed is relatively fast, which can make the sample precipitate in a short time, for example, in clinical examination, for the rapid centrifugation of blood samples, the angle type centrifuge can separate blood cells and plasma in a few minutes, the design of the inclination angle helps the blood cells to quickly settle at the bottom of the centrifuge tube, therefore the inclination angle of the upper end of the plurality of rotating supports 231 of the device is forty degrees, which can make the plurality of sample bottles be in a forty-degree state for centrifugation and placement, and the occupied space is relatively small, which can quickly separate cell fragments, bacterial precipitates and the like, and is a relatively ideal choice.

[0035] The above merely describes the embodiments of the present application and is not intended to limit the present application. The present application can be variously changed and modified by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the scope of the claims of the present application.

Claims

1. A kit that facilitates the placement of sample bottles, characterized in that: include; A kit for storing sample bottles (1); The kit (1) is internally provided with a centrifugal separation device (2) for centrifuging the sample bottles; The centrifugal separation device (2) comprises a driving mechanism (21), an auxiliary mechanism (22) and a rotating mechanism (23); The driving mechanism (21) is arranged inside the reagent box (1), and a plurality of the rotating mechanisms (23) are provided. The plurality of rotating mechanisms (23) are equidistantly arranged around the driving mechanism (21). The auxiliary mechanism (22) is arranged inside the reagent box (1), and the plurality of rotating mechanisms (23) and the auxiliary mechanism (22) are respectively in a coordinated rotation state. The reagent box (1) includes a reagent chamber (11) and a reagent cover (12). The reagent cover (12) is arranged on the reagent chamber (11) and is openable.

2. A kit for facilitating sample bottle placement according to claim 1, characterized in that: The driving mechanism (21) comprises a driving motor (211), a driving wheel (212), a driven wheel (213) and a belt (214); the driving motor (211) is arranged inside the reagent box (1); the driving wheel (212) is arranged on the output end of the driving motor (211); the driven wheel (213) is rotatably connected inside the reagent box (1) and is located beside the driving wheel (212); and the belt (214) is sleeved on the driving wheel (212) and the driven wheel (213).

3. A kit for facilitating sample bottle placement according to claim 1, characterized in that: The test kit (1) is provided with a control system for electrically connecting the drive motor (211), wherein the drive motor (211) is a DC speed-regulating motor. The control system is provided with a control display screen (215), wherein the control display screen (215) is a touch display screen operable to control the operation of the drive motor (211).

4. A kit for facilitating the placement of sample bottles according to claim 2, characterized in that: The auxiliary mechanism (22) comprises an auxiliary shaft (221) and an auxiliary bevel gear (222); the middle portion of the driven wheel (213) is hollow; the auxiliary shaft (221) is arranged on the reagent box (1) and the upper end thereof passes through the hollow portion of the driven wheel (213); and the auxiliary bevel gear (222) is arranged at the upper end of the auxiliary shaft (221).

5. The kit for facilitating sample bottle placement according to claim 1, wherein: The plurality of rotating mechanisms (23) each comprise a rotating bracket (231), a rotating shaft (232) and a rotating storage cylinder (233); the rotating bracket (231) is arranged at the upper end of the driven wheel (213); the upper end of the rotating bracket (231) is inclined; the rotating shaft (232) is arranged at the upper end of the rotating bracket (231) and faces inward; the rotating shaft (232) is rotatably connected to the rotating bracket (231); a rotating gear (234) adapted to and meshed with the auxiliary bevel gear (222) is sleeved on the rotating shaft (232); and the rotating storage cylinder (233) is arranged at the upper end of the rotating shaft (232).

6. A kit for facilitating the placement of sample bottles according to claim 5, characterized in that: A storage groove (235) is provided inside the rotating storage cylinder (233), and the storage groove (235) is configured to be compatible with a conventional sample bottle in size.

7. The kit for facilitating the placement of sample bottles according to claim 5, characterized in that: The inclination angle of the upper ends of the plurality of rotating brackets (231) is forty degrees.

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