Negative pressure ultrafiltration centrifuge tube device

Abstract

The utility model relates to the technical field of biological filtration, and particularly discloses a negative pressure ultrafiltration centrifuge tube device which comprises a negative pressure base, a negative pressure cover and an ultrafiltration bracket which are sequentially stacked and connected from bottom to top, the ultrafiltration support is provided with a plurality of placing holes arranged in a matrix, the placing holes are provided with ultrafiltration centrifuge tubes, a bottom frame is arranged in the negative pressure base, the bottom frame is provided with a plurality of groups of elastic limiting structures corresponding to the placing holes, and the elastic limiting structures clamp collecting tubes. The ultrafiltration centrifugal tubes and the corresponding collecting tubes are coaxially arranged, and at least part of each ultrafiltration centrifugal tube can extend into the corresponding collecting tube in the axial direction of the ultrafiltration centrifugal tube. Experiments such as liquid filtration, concentration, cell separation and desalination can be achieved through negative pressure, the application range is wide, compared with the prior art, the device has the advantages of being short in time and high in efficiency, the ultrafiltration membrane cannot be damaged or leaked, and cells can be effectively prevented from being damaged.

Description

TECHNICAL FIELD

[0001] The utility model relates to biological filtration technical field especially relates to a negative pressure ultrafiltration centrifugal tube device. BACKGROUND

[0002] The ultrafiltration centrifugal tube is a disposable filter device combining centrifugal force and ultrafiltration technology, which provides reliable data for the concentration, desalination and buffer exchange of protein samples in biological experiments. Compared with chromatography and dialysis, the ultrafiltration centrifugal tube is more gentle to molecules, does not require organic extraction and does not cause protein denaturation. The operation is fast, simple and efficient. While separating molecules, the concentration ratio is greatly improved, and the concentration efficiency is significantly improved. Ultrafiltration is mainly used for desalination, osmosis or buffer replacement; biological sample concentration and purification, including antigens, antibodies, enzymes, nucleic acids (single or double DNA / RNA samples), microorganisms, eluate, etc.; purification of macromolecular components in tissue culture extracts or cell lysates; removal of primers, connections or molecular markers from reaction mixtures; removal of proteins before HPLC.

[0003] However, the existing ultrafiltration centrifugal tube needs to be centrifuged and filtered by a rotary high-speed centrifuge, which takes a long time and has a limited number of centrifugations per time, resulting in low efficiency. In addition, high-speed centrifugal filtration can easily cause damage and leakage of the ultrafiltration membrane, and even cause abnormal phenomena such as cell damage. UTILITY MODEL CONTENT

[0004] The technical problem to be solved by the utility model is how to solve the problems of low efficiency and easy damage and leakage of the ultrafiltration membrane in the prior art.

[0005] To solve the above technical problems, the utility model provides a negative pressure ultrafiltration centrifugal tube device, which comprises a negative pressure base, a negative pressure cover and an ultrafiltration support connected in sequence from bottom to top.

[0006] The ultrafiltration support is provided with a plurality of placement holes arranged in a matrix, the placement holes are provided with ultrafiltration centrifugal tubes, the negative pressure base is provided with a chassis, the chassis is provided with a plurality of elastic limiting structures corresponding to the placement holes, the elastic limiting structures clasp collection tubes, the ultrafiltration centrifugal tubes and the corresponding collection tubes are coaxially arranged, and at least part of the ultrafiltration centrifugal tubes can extend into the collection tubes in the axial direction.

[0007] Further preferably, a sealing ring is embedded in the placement hole, and the ultrafiltration centrifugal tube can be connected to the sealing ring.

[0008] Further preferably, the sealing ring comprises a first abutting surface and a second abutting surface, the first abutting surface and the second abutting surface abut the outer surface and the inner surface of the ultrafiltration support respectively, and one end of the first abutting surface and the second abutting surface is connected to form an embedded groove.

[0009] Further preferably, the inner surface of the ultrafiltration support is further provided with at least one first skeleton and at least one second skeleton, and the first skeleton and the second skeleton are arranged intersectingly.

[0010] Further preferably, the negative pressure base is provided with a first cavity on one side of the ultrafiltration support, and the first cavity is provided with at least one convex structure for supporting the base, and the convex structure is configured to form a gap between the base and the negative pressure base.

[0011] Further preferably, the negative pressure base is provided with at least two convex columns near the edge of one side of the negative pressure cover, the negative pressure cover is provided with at least one cylindrical hole and at least one elliptical hole near the edge of one side of the negative pressure base, and the at least two convex columns are respectively inserted into the cylindrical hole and the elliptical hole.

[0012] Further preferably, the negative pressure cover is provided with a first step structure near one side of the ultrafiltration support, the ultrafiltration support is provided with a second step structure near one side of the negative pressure cover, and the first step structure and the second step structure are coupled to each other.

[0013] Further preferably, a sealing sheet is arranged between the first step structure and the second step structure.

[0014] Further preferably, the base is provided with a second cavity on one side of the ultrafiltration support, and a plurality of groups of the elastic limiting structures are arranged in the second cavity in a matrix, each group of the elastic limiting structures comprises at least two arc-shaped columns arranged on the same circle to limit and clamp the collection tube.

[0015] Further preferably, the base is further provided with a reinforcing rib connected to the convex surface of the arc-shaped column.

[0016] Compared with the prior art, the negative pressure ultrafiltration centrifugal tube device has the following advantages:

[0017] The utility model provides a negative pressure base, a negative pressure cover and an ultrafiltration support which are stacked and connected in sequence from bottom to top, and the placement holes arranged in a matrix can meet the filtration needs of multiple ultrafiltration centrifuge tubes at the same time, thereby improving operational efficiency. After the ultrafiltration centrifuge tubes are placed in the placement holes, an independent closed space is formed inside the negative pressure ultrafiltration centrifuge tube device. Under the action of negative pressure, the collection tube can independently collect the solution of each ultrafiltration centrifuge tube, thereby reducing the difference that may be caused by each tube affecting the results, and facilitating independent observation and inspection. In addition, the elastic limiting structure provided on the base frame can fasten the collection tube to the base frame under negative pressure. At the same time, when the collection tube is inserted and removed, the elastic limiting structure will not break and will not cause the collection tube to be squeezed. The utility model can realize liquid filtration, concentration, cell separation, desalination and other experiments through negative pressure, and has a wide range of applications. Compared with the existing method of centrifugal filtration through a rotary high-speed centrifuge, the utility model has the advantages of short time and high efficiency, and will not cause damage or leakage of the ultrafiltration membrane, and can effectively avoid cell damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a stereoscopic diagram of the negative pressure ultrafiltration centrifuge tube device of the present invention.

[0019] Figure 2 It is an explosion diagram of the negative pressure ultrafiltration centrifuge tube device of the present invention.

[0020] Figure 3 It is a structural schematic diagram of the negative pressure base of the utility model.

[0021] Figure 4 It is a structural schematic diagram of the negative pressure cover of the present invention.

[0022] Figure 5 It is a schematic diagram of the assembly of the negative pressure base and the negative pressure cover of the utility model.

[0023] Figure 6 It is a structural schematic diagram of the ultrafiltration support of the utility model.

[0024] Figure 7 It is a structural schematic diagram of the ultrafiltration support of the present invention from another perspective.

[0025] Figure 8 It is a cross-sectional view of the ultrafiltration support of the utility model.

[0026] Figure 9 This utility model Figure 8 Enlarged schematic diagram of point A in the middle.

[0027] Figure 10 It is an exploded schematic diagram of the negative pressure cover and the ultrafiltration support described in the present invention.

[0028] Figure 11 is the assembly diagram of the bottom frame and the collecting pipe.

[0029] Figure 12 is the explosion diagram of the bottom frame and the collecting pipe.

[0030] Figure 13 is the top view of the bottom frame.

[0031] Figure 14 is the top view of the negative pressure ultrafiltration centrifugal tube device.

[0032] Figure 15 is the sectional view of B-B section in the utility model. Figure 14

[0033] Figure 16 is the sectional view of C-C section in the utility model. Figure 14

[0034] Reference signs:

[0035] 10, negative pressure base; 101, first chamber; 102, connecting hole; 103, convex column; 104, convex structure;

[0036] 20, negative pressure cover; 201, cylindrical hole; 202, oval hole; 203, hollowed-out round corner; 204, first step structure;

[0037] 30, ultrafiltration support; 301, placing hole; 302, sealing ring; 302a, first abutting surface; 302b, second abutting surface; 302c, embedded groove; 303, first skeleton; 304, second skeleton; 305, second step structure;

[0038] 40, ultrafiltration centrifugal tube;

[0039] 50, bottom frame; 501, second chamber; 502, arc column; 503, through hole; 504, reinforcing rib;

[0040] 60, collecting pipe;

[0041] 70, sealing sheet. DETAILED DESCRIPTION

[0042] The specific implementation of the utility model will be described in further detail below in combination with the drawings and examples. The following examples are used to illustrate the utility model, but not to limit the scope of the utility model.

[0043] ​​In the description of the utility model, it should be understood that the utility model adopts the orientation or positional relation indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like based on the orientation or positional relation shown in the drawings, and it is only for the convenience of describing the utility model and simplifying the description, and therefore it cannot be understood as limiting the utility model, which indicates or implies that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the utility model.

[0044] The terms "first", "second", "third", etc. are used only for descriptive purposes and should not be construed as indicating or implying relative importance or an indicated number of technical features. Therefore, the features defined as "first", "second", etc. can explicitly or implicitly include at least one of the features. In the description of the utility model, the meaning of "multiple" is at least two, such as two, three, etc., unless otherwise specifically limited.

[0045] In addition, unless otherwise specifically defined and limited, the terms "mounting", "connection", "connection" should be broadly understood, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected, it can be mechanically connected, or it can be electrically connected, it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be the communication between two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0046] In the utility model, unless otherwise specifically defined and limited, the first feature is "on" or "under" the second feature, which can be direct contact between the first and second features, or indirect contact between the first and second features through an intermediate medium. Moreover, the first feature "above", "above" and "above" the second feature can be directly above or obliquely above the first feature, or only indicate that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" the second feature can be directly below or obliquely below the first feature, or only indicate that the horizontal height of the first feature is less than that of the second feature.

[0047] It should be noted that when an element is referred to as "fixed to" or "provided on" another element, it can be directly on another element or there can be a middle element. When an element is considered to be "connected" to another element, it can be directly connected to another element or there can be a middle element. The terms "vertical", "horizontal", "up", "down", "left", "right" and similar expressions used herein are only for the purpose of illustration, and do not indicate the only embodiment.

[0048] As shown in Figures 1-2 , Figures 14-16 , the embodiment provides a negative pressure ultrafiltration centrifuge tube device, which comprises a negative pressure base 10, a negative pressure cover 20, an ultrafiltration support 30, an ultrafiltration centrifuge tube 40, a base frame 50 and a collection tube 60, wherein the negative pressure base 10 is connected with a negative pressure system, and the embodiment can realize liquid filtration, concentration, cell separation, desalination and other experiments through negative pressure.

[0049] In some embodiments, the negative pressure base 10, the negative pressure cover 20 and the ultrafiltration support 30 are sequentially stacked and connected from bottom to top, the base frame 50 is arranged in the negative pressure base 10, the ultrafiltration support 30 is used for fixing the ultrafiltration centrifuge tube 40, and the base frame 50 is used for fixing the collection tube 60.

[0050] In some embodiments, the negative pressure base 10 is provided with a first cavity 101 on the side facing the ultrafiltration support 30, the side wall of the negative pressure base 10 is provided with a connecting hole 102 in communication with the first cavity 101, the connecting hole 102 is connected with the negative pressure system, and when the negative pressure system is configured to be started, the first cavity 101 can form a negative pressure state, so that the liquid filtration, concentration, cell separation, desalination and other experiments in the ultrafiltration centrifuge tube 40 can be realized.

[0051] In some embodiments, the ultrafiltration support 30 is provided with a plurality of placement holes 301 arranged in a matrix, and the ultrafiltration centrifuge tube 40 can be fixed in the placement hole 301 in the axial direction, so that the filtration use of a plurality of ultrafiltration centrifuge tubes 40 can be simultaneously satisfied, and the operation efficiency is improved.

[0052] In some embodiments, the base frame 50 is provided with a plurality of groups of elastic limiting structures corresponding to the placement holes 301, the elastic limiting structures clamping the collection tubes 60, the elastic limiting structures can fasten the collection tubes 60 on the base frame 50 under the negative pressure state, and at the same time, when the collection tubes 60 are inserted, the elastic limiting structures will not be broken, nor will the collection tubes 60 be squeezed and broken; the ultrafiltration centrifuge tube 40 and the corresponding collection tube 60 are coaxially arranged, and at least part of the ultrafiltration centrifuge tube 40 can extend into the collection tube 60 in the axial direction, so that the collection tube 60 can effectively collect the substances filtered out of the ultrafiltration centrifuge tube 40.

[0053] Compared with centrifugal filtration by a rotary high-speed centrifuge, the embodiment has the advantages of short time and high efficiency, and will not cause damage and leakage of the ultrafiltration membrane, and can effectively avoid damage of the cells.

[0054] In some embodiments, in order to form an independent and sealed space in the negative pressure ultrafiltration centrifuge tube device during work, for this purpose, as shown in Figures 6-9As shown, the sealing ring 302 is embedded in the placement hole 301, the ultrafiltration centrifugal tube 40 can be connected to the sealing ring 302, and the ultrafiltration centrifugal tube 40 is connected with the sealing ring 302 in an interference fit. In this way, when the ultrafiltration centrifugal tube 40 is connected to the sealing ring 302, the placement hole 301 can be completely sealed, so that external gas cannot enter the inside and affect the experimental results.

[0055] In other embodiments, the sealing ring 302 is preferably a medical-grade silicone ring to avoid affecting the specimen.

[0056] In addition, the sealing ring 302 can be elastically deformed without damaging the ultrafiltration centrifugal tube 40 during the plugging process, avoiding damage caused by friction between the ultrafiltration centrifugal tube 40 and the ultrafiltration support 30 during the plugging process. In addition, the sealing ring 302 can be connected with the ultrafiltration centrifugal tube 40 in an interference fit, and the sealing ring 302 tightly holds the ultrafiltration centrifugal tube 40, further improving the reliability of the experimental results.

[0057] In some embodiments, the sealing ring 302 includes a first abutting surface 302a and a second abutting surface 302b, which respectively abut with the outer surface and the inner surface of the ultrafiltration support 30. One end of the first abutting surface 302a and the second abutting surface 302b is connected to form an embedded groove 302c. In this way, the sealing ring 302 adopts an embedded structure which is more firm and durable. When the negative pressure ultrafiltration centrifugal tube device is in a negative pressure state, the pressure on the outside of the sealing ring 302 is greater than the pressure on the inside. At this time, the first abutting surface 302a is in close contact with the outer surface of the ultrafiltration support 30 to form a good sealing effect. When the ultrafiltration centrifugal tube 40 is pulled out, the second abutting surface 302b is in close contact with the inner surface of the ultrafiltration support 30 to limit the sealing ring 302. The sealing ring 302 will not be separated from the ultrafiltration support 30 along with the ultrafiltration centrifugal tube 40. A new ultrafiltration centrifugal tube 40 can be used for negative pressure filtration again without replacing the sealing ring 302, which is convenient and fast.

[0058] In some embodiments, the inner surface of the ultrafiltration support 30 is further provided with at least one first skeleton 303 and at least one second skeleton 304, and the first skeleton 303 and the second skeleton 304 intersect. The first skeleton 303 and the second skeleton 304 can effectively enhance the structural strength of the ultrafiltration support 30. On the one hand, the ultrafiltration support 30 will not deform under the action of negative pressure. On the other hand, the ultrafiltration support 30 will not be damaged when the ultrafiltration centrifugal tube 40 is inserted or pulled out.

[0059] In other embodiments, the first skeleton 303 and the second skeleton 304 intersect perpendicularly.

[0060] In other embodiments, the ultrafiltration support 30 is an aluminum alloy support, which can improve the structural strength of the ultrafiltration support 30 in cooperation with the skeleton.

[0061] In some embodiments, as shown in Figure 3 In some embodiments, the first chamber 101 of the negative pressure base 10 is provided with at least one protruding structure 104 for supporting the base frame 50, which is configured to form a gap between the base frame 50 and the negative pressure base 10, effectively raising the height of the base frame 50, enhancing the internal gas flow and improving the filtering effect.

[0062] In some embodiments, the base frame 50 is provided with a second chamber 501 on the side facing the ultrafiltration support 30, and the base frame 50 is provided with a through hole 503 communicating the first chamber 101 and the second chamber 501, which more effectively forms air pressure flow and further enhances the internal gas flow.

[0063] In some embodiments, to further improve the effect of air flow, the inner corner of the negative pressure cover 20 is provided with a hollow round corner 203.

[0064] In some embodiments, as shown in Figures 3-5 In some embodiments, the edge of the negative pressure base 10 near the negative pressure cover 20 is provided with at least two protruding columns 103, the edge of the negative pressure cover 20 near the negative pressure base 10 is provided with at least one cylindrical hole 201 and at least one elliptical hole 202, and the at least two protruding columns 103 are respectively inserted into the cylindrical hole 201 and the elliptical hole 202, which can realize precise insertion of the negative pressure base 10 and the negative pressure cover 20 and limit the rotational pair between the negative pressure base 10 and the negative pressure cover 20.

[0065] Specifically, the embodiment preferably has two protruding columns 103, which are respectively arranged on opposite sides of the negative pressure base 10, and the cylindrical hole 201 and the elliptical hole 202 are also respectively arranged on opposite sides of the negative pressure cover 20. When the negative pressure base 10 and the negative pressure cover 20 are connected, one of the protruding columns 103 is inserted into the cylindrical hole 201, and the other protruding column 103 is inserted into the elliptical hole 202. The setting of the elliptical hole 202 enables the negative pressure cover 20 to be more flexible to the negative pressure base 10, improving the assembly efficiency.

[0066] In some embodiments, as shown in Figure 10 In some embodiments, the negative pressure cover 20 is provided with a first step structure 204 near the ultrafiltration support 30, and the ultrafiltration support 30 is provided with a second step structure 305 near the negative pressure cover 20, and the first step structure 204 and the second step structure 305 are coupled to each other, which makes the installation of the ultrafiltration support 30 and the negative pressure cover 20 more convenient and flexible, and does not deviate under negative pressure.

[0067] In some embodiments, to further improve the sealing of the connection between the negative pressure cover 20 and the ultrafiltration support 30, a sealing sheet 70 is arranged between the first step structure 204 and the second step structure 305, so that, under the negative pressure state, the ultrafiltration support 30 is subjected to a downward force, and the first step structure 204 and the second step structure 305 are tightly connected under the action of the sealing sheet 70, forming a sealed state.

[0068] In other embodiments, the sealing sheet 70 is preferably a medical silica gel sheet to avoid contamination of the specimen.

[0069] In some embodiments, the thickness of the sealing sheet 70 is 1-2 mm, preferably 1.5 mm.

[0070] In some embodiments, the negative pressure cover 20 is a plexiglass negative pressure cover, which has the advantages of transparency and toughness, facilitating observation and improving the cooperation between the negative pressure cover 20 and the ultrafiltration support 30.

[0071] In some embodiments, as shown in Figures 11-13 The bottom frame 50 is provided with a second chamber 501 on the side facing the ultrafiltration support 30, and a plurality of sets of elastic limiting structures are arranged in the second chamber 501 in a matrix, wherein each set of elastic limiting structures can independently fix one collection tube 60, so as to avoid displacement of the collection tube 60, and at the same time, reduce the possible differential influence of each collection tube 60 on the result, facilitating independent observation and inspection.

[0072] In some embodiments, each set of elastic limiting structures includes at least two arc-shaped columns 502, and the present embodiment preferably includes three arc-shaped columns 502, which are arranged on the same circumference and can limit and clamp the collection tube 60, so as to tightly fix the collection tube 60 to the bottom frame 50 under the negative pressure state.

[0073] In some embodiments, when the collection tube 60 is inserted, in order to effectively prevent the arc-shaped column 502 from being broken or the collection tube 60 from being squeezed and broken, the bottom frame 50 is further provided with a reinforcing rib 504 connected with the convex surface of the arc-shaped column 502, thereby enhancing the structural strength of the arc-shaped column 502.

[0074] The working process of the utility model is as follows: please combine Figures 1-16In use, the collecting pipe 60 is first fixed on the elastic limiting structure of the chassis 50, and then the chassis 50 is placed on the negative pressure base 10, the cylindrical hole 201 and the elliptical hole 202 of the negative pressure cover 20 are respectively inserted into the convex columns 103 at two ends of the negative pressure base 10, the ultrafiltration centrifugal tube 40 is connected to the sealing ring 302, the second step structure 305 of the ultrafiltration support 30 is connected to the first step structure 204 of the negative pressure cover 20, the position of the ultrafiltration centrifugal tube 40 is adjusted so that it can be at least partially extended into the collecting pipe 60 in the axial direction, the external negative pressure system is connected to the connecting hole 102, and after the negative pressure system is started, the substances in the ultrafiltration centrifugal tube 40 can realize liquid filtration, concentration, cell separation, desalination and other experiments under the negative pressure state.

[0075] In conclusion, the negative pressure ultrafiltration centrifugal tube device provided by the utility model can realize liquid filtration, concentration, cell separation, desalination and other experiments through negative pressure, has a wide range of applications, has the advantages of short time and high efficiency compared with the existing centrifugal filtration mode through a rotary high-speed centrifuge, and will not cause the ultrafiltration membrane to be damaged and leak, so that the cells can be effectively prevented from being damaged.

[0076] The above only describes the preferred embodiments of the utility model, and it should be noted that, for ordinary technical personnel in the technical field, without departing from the technical principles of the utility model, a number of improvements and replacements can be made, and these improvements and replacements should also be regarded as the protection range of the utility model. The basic principles, main features and advantages of the utility model are shown and described above, and for those skilled in the art, it is obvious that the utility model is not limited to the details of the above preferred embodiments, the embodiments should be regarded as exemplary and non-limiting, the scope of the utility model is defined by the appended claims rather than the above description, and therefore all changes falling within the meaning and range of the equivalent elements of the claims should be included in the utility model.

[0077] In addition, it should be understood that, although the present specification is described in terms of embodiments, not every embodiment contains only one independent technical solution, and the description manner of the specification is only for the sake of clarity, and those skilled in the art should regard the specification as a whole, and the technical solutions in the embodiments can also be properly combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A negative pressure ultrafiltration centrifuge tube device, characterized in that: It includes a negative pressure base, a negative pressure cover and an ultrafiltration support which are stacked and connected in sequence from bottom to top; The ultrafiltration bracket is provided with a plurality of placement holes arranged in a matrix, and the placement holes are provided with ultrafiltration centrifuge tubes. A base frame is provided in the negative pressure base, and the base frame is provided with a plurality of groups of elastic limiting structures corresponding to the placement holes. The elastic limiting structures clamp the collection tubes, and the ultrafiltration centrifuge tubes are coaxially arranged with the corresponding collection tubes, and at least part of the ultrafiltration centrifuge tubes can extend into the collection tubes along their axial direction.

2. A negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: A sealing ring is embedded in the placement hole, and the ultrafiltration centrifuge tube can be connected to the sealing ring.

3. A negative pressure ultrafiltration centrifuge tube device according to claim 2, characterized in that: The sealing ring includes a first abutting surface and a second abutting surface, the first abutting surface and the second abutting surface respectively abut against the outer surface and the inner surface of the ultrafiltration support, and one end of the first abutting surface and the second abutting surface are connected to form an embedding groove.

4. A negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: The inner surface of the ultrafiltration support is further provided with at least one first skeleton and at least one second skeleton, and the first skeleton and the second skeleton are arranged to intersect.

5. A negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: A first chamber is provided on a side of the negative pressure base facing the ultrafiltration support. The first chamber is provided with at least one protruding structure for supporting the base frame. The protruding structure is configured to form a gap between the base frame and the negative pressure base.

6. A negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: The edge of the negative pressure base close to the negative pressure cover is provided with at least two protrusions, and the edge of the negative pressure cover close to the negative pressure base is provided with at least one cylindrical hole and at least one elliptical hole, and the at least two protrusions are respectively plugged into the cylindrical hole and the elliptical hole.

7. A negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: A first step structure is provided on a side of the negative pressure cover close to the ultrafiltration support, and a second step structure is provided on a side of the ultrafiltration support close to the negative pressure cover. The first step structure and the second step structure are coupled to each other.

8. A negative pressure ultrafiltration centrifuge tube device according to claim 7, characterized in that: A sealing sheet is provided between the first step structure and the second step structure.

9. The negative pressure ultrafiltration centrifuge tube device according to claim 1, characterized in that: A second chamber is provided on the side of the base frame facing the ultrafiltration support, and multiple groups of elastic limiting structures are arranged in a matrix in the second chamber. Each group of elastic limiting structures includes at least two arc columns, and the at least two arc columns are placed on the same circumference so as to be able to limit and clamp the collection tube.

10. The negative pressure ultrafiltration centrifuge tube device according to claim 9, characterized in that: The base frame is further provided with reinforcing ribs, and the reinforcing ribs are connected to the convex surface of the arc column.

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