Fermentation container culture medium monitoring device and automatic sample adding fermentation system

Abstract

The embodiment of the utility model provides a fermentation container culture medium monitoring device and an automatic sample adding fermentation system. The fermentation container culture medium monitoring device comprises a fermentation container; the sampling mechanism comprises a filtering part and a sampling communicating pipe, the filtering part is arranged in the fermentation container, the sampling communicating pipe is communicated with the filtering part, and the filtering part is used for obtaining a liquid sample in the fermentation container; the analysis detector is connected with the sampling communicating pipe, and the analysis detector is used for detecting the content of components in the liquid sample; and the purging mechanism is connected with the sampling communicating pipe and is used for purging the sampling mechanism through the sampling communicating pipe. According to the embodiment, the liquid sample in the fermentation container is obtained through the sampling mechanism, and the component content in the liquid sample is detected through the analysis detector, so that manual sampling and detection are not needed, the detection efficiency can be improved, and the monitoring accuracy is improved; and the risk of bacterial contamination can be reduced through the arrangement of the purging mechanism.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of microbial fermentation, especially to a fermentation container culture medium monitoring device and an automatic sample adding fermentation system. BACKGROUND

[0002] The key link of synthetic biology is to realize target product by fermentation chassis cell, and the key equipment is the fermentation container such as fermentation tank, culture bag. The culture medium needs to provide the required substances such as carbon source, nitrogen source, vitamin, etc. to maintain normal metabolism during the fermentation culture process of chassis cell or microorganism.

[0003] During different stages of fermentation, the content of various components in the culture medium needs to be supplemented in time according to the fermentation situation. For example, glucose is a good carbon source because of its low industrial cost and easy utilization by microorganisms or cells, but the glucose concentration needs to be controlled to maintain the growth of chassis cell or microorganism during the fermentation culture process. Similar to the human body, the blood sugar concentration needs to be controlled every day, and the food sugar source needs to be supplemented to maintain the blood sugar concentration, which cannot be too high or too low. During the fermentation culture process, the glucose concentration consumed by microbial metabolism changes all the time. At present, the glucose concentration needs to be monitored by manually taking sample from the fermentation container, then detecting the glucose concentration in the sample by biochemical analyzer or glucose analyzer, and then manually controlling the glucose concentration according to the fermentation process.

[0004] However, such operation is complex and difficult to quickly detect results and supplement the corresponding culture medium components according to the results. In addition, the manual sampling method also increases the risk of contamination. UTILITY MODEL CONTENT

[0005] Therefore, in order to overcome at least part of the defects and deficiencies in the prior art, the utility model embodiment provides a fermentation container culture medium monitoring device and an automatic sample adding fermentation system.

[0006] Specifically, in one aspect, the fermentation container culture medium monitoring device provided by the utility model embodiment comprises: a fermentation container; a sampling mechanism, the sampling mechanism comprising a filter and a sampling communication pipe, the filter being arranged in the fermentation container, the sampling communication pipe communicating the filter, and the filter being used to obtain a liquid sample in the fermentation container; an analysis detector connected to the sampling communication pipe, the analysis detector being used to detect the content of components in the liquid sample; and a purging mechanism connected to the sampling communication pipe and used to purge the sampling mechanism through the sampling communication pipe.

[0007] In one specific embodiment of the utility model, the filter is a sampling sintered part, and the sampling sintered part is provided with a filtering through hole.

[0008] In one specific embodiment of the present application, the filter through hole has a pore size ranging from 0.1 to 0.22 microns.

[0009] In one specific embodiment of the present application, the sampling mechanism further comprises: a sample container connected to the sampling communication pipe; and a sampling driving member connected between the analysis detector and the sample container, the sampling driving member being used to sample from the sample container to the analysis detector.

[0010] In one specific embodiment of the present application, the purging mechanism comprises: an air filter, one end of the air filter being provided with a compressed air inlet, and the other end being communicated with the sampling communication pipe.

[0011] In one specific embodiment of the present application, the fermentation container culture medium monitoring device further comprises: a first valve connected between the purging mechanism and the sampling communication pipe; a second valve connected between the sampling communication pipe and the filter; and a third valve connected between the sample container and the sampling communication pipe.

[0012] On the other hand, the automatic sampling fermentation system provided by the embodiments of the present application comprises: the fermentation container culture medium monitoring device as described above; a feeding mechanism connected to the fermentation container; and a control system, the analysis detector and the feeding mechanism being electrically connected to the control system, the control system being used to control the feeding mechanism to supplement glucose into the fermentation container according to the detection result of the analysis detector.

[0013] In one specific embodiment of the present application, the feeding mechanism comprises: a feeding buffer member, the feeding buffer member being used to accommodate glucose supplement solution; a metering member connected to the feeding buffer member; and a feeding driving member connected between the feeding buffer member and the fermentation container, and the feeding driving member being electrically connected to the control system.

[0014] In one specific embodiment of the present application, the number of the fermentation containers is multiple, the number of the sampling mechanisms is multiple, the number of the metering members is multiple, and the number of the feeding driving members is multiple, one-to-one correspondence between the multiple sampling mechanisms and the multiple fermentation containers, the multiple sampling mechanisms being connected to the analysis detector, one-to-one correspondence between the multiple feeding driving members and the multiple fermentation containers, one-to-one correspondence between the multiple metering members and the multiple feeding driving members, and the multiple metering members being connected to the feeding buffer member.

[0015] In one specific embodiment of the present application, the analysis detector and the control system are arranged in a clean environment.

[0016] From the above, the fermentation container culture medium monitoring device provided by the embodiments of the present application comprises a fermentation container, a sampling mechanism, an analysis detector and a purging mechanism, the sampling mechanism comprises a filter and a sampling communication pipe, the filter is arranged in the fermentation container, and the sampling communication pipe is connected with the filter, so that the liquid sample in the fermentation container can be obtained through the sampling mechanism, and the component content in the liquid sample can be detected through the analysis detector, so that manual sampling and detection are not needed, the detection efficiency can be improved, and the monitoring accuracy is improved; and the sampling communication pipe is connected with the purging mechanism through the purging mechanism, the sampling mechanism is purged through the purging mechanism, and the risk of bacterial contamination is reduced. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical scheme of the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained according to these drawings without creative labor for those skilled in the art.

[0018] Figure 1 A structure schematic view of the fermentation container culture medium monitoring device provided by the embodiments of the present application.

[0019] Figure 2 A structure schematic view of the fermentation container culture medium monitoring device provided by the embodiments of the present application. Figure 1 A structure schematic view of the fermentation container culture medium monitoring device provided by the embodiments of the present application.

[0020] Figure 3 Another structure schematic view of the fermentation container culture medium monitoring device provided by the embodiments of the present application.

[0021] Figure 4 A structure schematic view of the automatic sample adding fermentation system provided by the embodiments of the present application.

[0022] Figure 5 A structure schematic view of the automatic sample adding fermentation system provided by the embodiments of the present application.

[0023] Main element label:

[0024] 10, fermentation container; 11, first valve; 12, second valve; 13, third valve; 20, sampling mechanism; 21, filter; 211, filter through hole; 22, sampling communication pipe; 23, sample container; 24, sampling driving piece; 30, air filter; 40, analysis detector; 50, feeding mechanism; 51, feeding buffer; 52, metering piece; 53, feeding driving piece; 54, feeding control valve; 60, control system. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the present application clearer, the technical scheme in the embodiments of the present application will be described clearly and completely below with reference to the drawings. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments described in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the present application.

[0026] In the embodiments of the present application, the description such as "first", "second" and the like is only for the purpose of description, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" can explicitly or implicitly include at least one of the features.

[0027] Referring to Figures 1 to 3 , the embodiments of the present application provide a fermentation vessel culture medium monitoring device. As shown in Figure 1 , the fermentation vessel culture medium monitoring device may, for example, include a fermentation vessel 10, a sampling mechanism 20, an analysis detector 40 and a purging mechanism.

[0028] Specifically, referring to Figure 1 and Figure 2 , the fermentation vessel 10 may, for example, be a device used in industry to carry out microbial fermentation. The sampling mechanism 20 may, for example, include a filter 21 and a sampling communication pipe 22, the filter 21 may, for example, be arranged in the fermentation vessel 10, and the sampling communication pipe 22 is connected to the filter 21, the filter 21 is used to obtain a liquid sample in the fermentation vessel 10, and the liquid sample obtained by the filter 21 may, for example, be transmitted into the sampling communication pipe 22. The analysis detector 40 may, for example, be a glucose analyzer, and of course may, for example, be other component analysis detectors, the analysis detector 40 is connected to the sampling communication pipe 22, the analysis detector 40 may, for example, obtain the liquid sample in the filter 21 through the sampling communication pipe 22, and is used to detect the component content in the liquid sample.

[0029] The existing method is easy to cause the loss of the cells or microorganisms in the sampling process when analyzing the components such as glucose and glycerol in the fermentation container, and is easy to cause pollution. Before the analyzer detects the sample, the bacteria bodies need to be removed by centrifugation manually, and the supernatant is taken for detection to avoid the cells or mycelium from entering the analyzer to affect the use of the equipment. The filter 21 provided in the embodiment can directly intercept the microbial bodies or mycelium, which is similar to replacing the manual centrifugation operation, so that the liquid sample is similar to the fermentation supernatant, thereby simplifying the sampling process, avoiding damage to the detection equipment, and being applicable to different types of fermentation container experiments. Through the setting of the sampling mechanism 20 and the analysis detector 40, the liquid sample in the fermentation container 10 can be obtained through the sampling mechanism 20, and the component content in the liquid sample can be detected through the analysis detector 40, so that manual sampling and detection are not required, the detection efficiency can be improved, and the monitoring accuracy can be improved.

[0030] The blowing mechanism can be connected to the sampling communication pipe 22, for example, for blowing the sampling mechanism 20 through the sampling communication pipe 22. The blowing mechanism can blow the sampling communication pipe 22 and the filter 21, for example, by sterile gas. Through the setting of the blowing mechanism, sterile sampling can be realized, and the risk of contamination can be reduced. Before the sampling mechanism 20 samples, the blowing mechanism can blow the filter 21, for example, by sterile gas, to prevent the fermentation liquid from sticking to the surface and causing blockage so that the sample cannot be taken out. The sterile gas can effectively blow away the surface attachments from the forward blowing, so that the surface attachments of the filter 21 can be blown away to prevent sampling blockage. After the blowing is completed, the sampling mechanism 20 samples, and after the sampling is completed, the blowing mechanism can blow out the residual liquid in the sampling communication pipe 22, and the pipeline sample is emptied to prevent the pipeline residual from affecting the next analysis and detection value. The pipeline is guaranteed to have a positive pressure environment before and after the blowing is completed, and the risk of contamination during the sampling process is reduced.

[0031] In one embodiment of the embodiment, the blowing mechanism can include an air filter 30, one end of the air filter 30 is provided with a compressed air inlet, and the other end is communicated with the sampling communication pipe 22. The compressed air enters the air filter 30 through the compressed air inlet, and the air filter 30 forms sterile air after filtration. The sterile air enters the sampling mechanism 20 through the sampling communication pipe 22.

[0032] The fermentation container culture medium monitoring device provided by the embodiment of the utility model includes fermentation container 10, sampling mechanism 20, analysis detector 40 and purging mechanism, sampling mechanism 20 includes filter piece 21 and sampling communication pipe 22, by setting filter piece 21 in fermentation container 10, sampling communication pipe 22 is connected through filter piece 21, so that the liquid sample in fermentation container 10 can be obtained through sampling mechanism 20, and the cost content in the liquid sample is detected through analysis detector 40, so that manual sampling and detection are not needed, the detection efficiency can be improved, and the accuracy of monitoring is improved; and sampling communication pipe 22 is communicated through purging mechanism, sampling mechanism 20 is purged through purging mechanism, so that the risk of bacterial contamination is reduced.

[0033] Referring to Figure 2 In one embodiment of the embodiment, the filter piece 21 may, for example, be a sampling sintered piece, the sampling sintered piece may, for example, be a rod-shaped structure, and the filter piece 21 is small in size and does not affect the stirring of the fermentation container 10. The sampling sintered piece is provided with a filtering through hole 211, and the pore size of the filtering through hole 211 ranges from 0.1 to 0.22 μm. The material of the filter piece 21 may, for example, be metal or ceramic material. Since the diameters of cells, bacteria and spores, fungi and spores usually reach several microns, the filter piece 21 with the pore size in the above range can not only avoid the loss of the culture target in the fermentation container during sampling, but also effectively prevent external microorganisms from entering the fermentation container.

[0034] Referring to Figure 3 The sampling mechanism 20 may, for example, further include a sample container 23 and a sampling driving piece 24. The sample container 23 may, for example, be a sampling bottle, and the size of the sample container 23 can be configured according to the size of the sampling amount; the sampling driving piece 24 may, for example, be a control pump. The sample container 23 is connected to the sampling communication pipe 22, and the sampling driving piece 24 is connected between the analysis detector 40 and the sample container 23. The sampling process may, for example, be based on the pressure in the fermentation container 10 to naturally press the liquid sample in the filter piece 21 out of the sample container 23 connected to the sampling communication pipe 22, and when sampling is needed, the liquid sample may, for example, be sucked to the analysis detector 40 through the sampling driving piece 24 for detection and analysis. The excess liquid sample may, for example, be pumped into a waste bottle to ensure that the sample container 23 is free of residual liquid sample.

[0035] In the embodiment, the fermentation vessel culture medium monitoring device may, for example, further comprise a first valve 11, a second valve 12 and a third valve 13. The first valve 11 is connected between the purging mechanism and the sampling communication pipe 22, and may, for example, be a sterile air control valve for controlling the opening and closing of the purging mechanism, i.e. for controlling whether sterile gas enters the sampling mechanism 20. The second valve 12 is connected between the sampling communication pipe 22 and the filter 21, and is a sampling communication pipe 22 control valve for controlling the liquid sample in the filter 21 to enter the sampling communication pipe 22. The third valve 13 is connected between the sample container 23 and the sampling communication pipe 22, and is a sampling communication pipe 22 control valve, and the third valve 13 is used for controlling the liquid sample in the sampling communication pipe 22 to enter the sample container 23, i.e. the second valve 12 and the third valve 13 are used for controlling the sampling of the sample, and the sampling amount of the liquid sample can be controlled by controlling the opening and closing time of the second valve 12 and the third valve 13.

[0036] Referring to Figure 4 The embodiment of the utility model may, for example, further provide an automatic sample adding fermentation system. The automatic sample adding fermentation system may, for example, comprise the fermentation vessel culture medium monitoring device provided by the above embodiment, a feeding mechanism 50 and a control system 60. The feeding mechanism 50 is connected to the fermentation vessel 10, and the feeding mechanism 50 and the analytical detector 40 are respectively electrically connected to the control system 60, and the control system 60 is used for controlling the feeding mechanism 50 to supplement glucose into the fermentation vessel 10 according to the detection result of the analytical detector 40. The analytical detector 40 transmits the detection result to the control system 60, and the control system 60 may, for example, compare the detection result with the component content threshold value, and control the feeding mechanism 50 to supplement a specified amount of glucose into the fermentation vessel 10 according to the requirement, so as to ensure that the glucose content in the fermentation vessel 10 reaches the fermentation requirement.

[0037] In the embodiment, the control system 60 can be electrically connected to the sampling drive 24, and the control system 60 can control the sampling drive 24 to automatically sample at a sampling frequency. For example, the control system 60 can monitor the ingredient content of the liquid sample in the fermentation vessel 10 by controlling the sampling frequency of the sampling drive 24, and the interval of the sampling frequency can be, for example, 15 min-180 min, and the embodiment is not limited thereto. According to the sampling interval signal setting, for example, 20 min sampling once, before sampling every 20 min, the control system 60 can send a sampling signal to the sampling drive 24, for example, the first valve 11 and the second valve 12 are in the open state, the filtered sterile gas passes through the sampling communication pipe 22 to blow the filter 21, and the blowing time can be, for example, 5-30 s, then the first valve 11 is closed and the third valve 13 is opened, the tank pressure pushes the liquid sample out of the sampling communication pipe 22 for, for example, 1-10 s, then the second valve 12 is closed and the first valve 11 is opened, so that the sterilization gas is emptied from the sampling communication pipe 22, and the blowing time can be, for example, 1-3 s, then the first valve 11 and the third valve 13 are closed, and the sampling drive 24 can suck the liquid sample in the sample container 23 to detect the glucose concentration. The amount of liquid sample in the whole process can be controlled according to the sterilization gas pressure, the fermentation vessel pressure and the opening time of each valve, so as to ensure that the sampling liquid sample is small.

[0038] Further, the feeding mechanism 50 can include, for example, a feeding buffer 51, a metering element 52 and a feeding drive 53. The feeding buffer 51 is used to contain the glucose supplement liquid, and the feeding buffer 51 can be, for example, a glucose feeding tank. The metering element 52 can be, for example, a mass meter or a flow meter, and the metering element 52 is connected to the feeding buffer 51 for metering the weight of the glucose supplemented into the fermentation vessel 10. The feeding drive 53 is connected between the feeding buffer 51 and the fermentation vessel 10, and the feeding drive 53 is electrically connected to the control system 60. The feeding drive 53 and the fermentation vessel 10 can be further provided with a feeding control valve 54.

[0039] For example, the control system 60 may, for example, compare the detection result of the analysis detector 40 with a set threshold value, if the detection result is greater than or equal to the threshold value, no feeding is required, if the detection result is less than the threshold value, the control system 60 may, for example, control the feeding mechanism to feed, specifically, the control system 60 may, for example, control the feeding drive 53 to feed, the amount of feeding is controlled by the metering element 52, specifically, for example, the volume value V1 of the metering element 52 is collected, the control system 60 calculates the target glucose feeding value V, the tank pressure of the feeding buffer 51, i.e. the glucose feeding tank, is higher than the tank pressure of the fermentation container 10, the feeding control valve 54 is opened, the metering element 52 may, for example, accumulate the glucose feeding flow value, when the metering element 52 reaches the V2 value, the feeding control valve 54 is closed, wherein V2 = V1 + V, when the metering element 52 reaches V2, it indicates that the target feeding amount has been fed, until the next sampling frequency detection, the above process is repeated to complete the glucose online control and automatic feeding, until the glucose control is maintained to the target control value to the stable state.

[0040] Through the setting of the control system 60 and the feeding mechanism, the control system 60 can automatically calculate whether feeding is required or the weight of the feeding according to the detection result of the analysis detector 40, realize process unmanned control, can reduce the risk of manual process operation failure and less manual, and can well control the glucose concentration, effectively control the microbial metabolism, for example, excessive glucose feeding produces acetic acid and reduces the target protein yield; furthermore, compared with the traditional operation offline sampling, the present system realizes the advantages of small sampling frequency and automatic sampling at night, which is beneficial to the control of glucose concentration in the early stage of process development. Microbial metabolism consumes glucose rapidly, if the sampling interval is long, there may be a case that the process has consumed all the glucose, therefore, the present embodiment can reduce the glucose sampling detection interval, can immediately follow up the glucose metabolism consumption situation, and is beneficial to the improvement of target process yield.

[0041] Referring to Figure 5In one embodiment of the present embodiment, the number of fermentation containers 10 may, for example, be multiple, the number of sampling mechanisms 20 may, for example, be multiple, the number of metering members 52 is multiple, and the number of feeding driving members 53 is multiple. The multiple sampling mechanisms 20 are respectively connected to the multiple fermentation containers 10 one by one, the multiple sampling mechanisms 20 are respectively connected to the analysis and detection instrument 40, the multiple feeding driving members 53 are respectively connected to the multiple fermentation containers 10 one by one, the multiple metering members 52 are respectively connected to the multiple feeding driving members 53 one by one, and the multiple metering members 52 are also connected to the feeding buffer 51. In the present embodiment, the fermentation containers 10, the feeding buffer 51, the filtering member 21, the sampling communication pipe 22, and the blowing mechanism are arranged in the general area, and the analysis and detection instrument 40, the control system 60, and the sample container 23 are arranged in the clean environment. Each fermentation container 10 is independently sampled into the corresponding sample container 23, and then the independent sampling driving member 24 is configured to suck the liquid sample in the sample container 23 into the analysis and detection instrument 40, each sampling driving member 24 can sample at the same time, realize that multiple groups of samples analyze the glucose concentration value together, and the analysis and detection instrument 40 feeds back multiple groups of detection results to the control system 60, and the control system 60 controls the feeding according to the multiple groups of detection results.

[0042] The production fermentation container is large in space and in the general area, the analysis and detection, and the control system 60 is arranged in the clean area, in this case, an independent sampling communication pipe 22 needs to be designed to reduce the sampling amount and the pipe length, the long sampling communication pipe 22 can be solved by subsequent compressed air blowing to the sample container 23, the long distance sampling problem is solved, multiple groups of tanks share one group of analysis and detection instruments 40 are realized, and the glucose addition value is controlled through the independent metering member 52, only one feeding buffer 51 needs to be matched, and the investment cost can be saved.

[0043] In addition, it can be understood that the foregoing various embodiments are only exemplary descriptions of the present application, and under the premise that the technical features do not conflict, the structures are not contradictory, and the purpose of the application is not violated, the technical solutions of the various embodiments can be arbitrarily combined and used.

[0044] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A fermentation vessel broth monitoring apparatus, characterized by, The application relates to a fermentation container medium monitoring device. The fermentation container (10) comprises a sampling mechanism (20) and an analysis detector (40). The sampling mechanism (20) comprises a filter (21) and a sampling communication pipe (22), wherein the filter (21) is arranged in the fermentation container (10), and the sampling communication pipe (22) is connected to the filter (21) and used for obtaining a liquid sample in the fermentation container (10). The analysis detector (40) is connected to the sampling communication pipe (22) and used for detecting the content of components in the liquid sample. The fermentation container (10) further comprises a blowing mechanism connected to the sampling communication pipe (22) and used for blowing the sampling mechanism (20) through the sampling communication pipe (22).

2. The fermentation vessel culture medium monitoring apparatus of claim 1, wherein, The filter (21) is a sampling sintered part, and a filtering through hole (211) is arranged on the sampling sintered part.

3. The fermentation vessel culture medium monitoring apparatus of claim 2, wherein, The filtering through hole (211) has a pore size ranging from 0.1 to 0.22 mu m.

4. The fermentation vessel broth monitoring apparatus of claim 1, wherein, The sampling mechanism (20) further comprises a sample container (23) connected to the sampling communication pipe (22) and a sampling driving part (24) connected between the analysis detector (40) and the sample container (23) and used for sampling from the sample container (23) to the analysis detector (40). The blowing mechanism comprises an air filter (30) having one end provided with a compressed air inlet and the other end connected to the sampling communication pipe (22). The fermentation container (10) further comprises a first valve (11) connected between the blowing mechanism and the sampling communication pipe (22), a second valve (12) connected between the sampling communication pipe (22) and the filter (21), and a third valve (13) connected between the sample container (23) and the sampling communication pipe (22).

5. The fermentation vessel culture medium monitoring apparatus of claim 1, wherein, The application further relates to a fermentation container medium monitoring device. The fermentation container (10) comprises a feeding mechanism (50).

6. The fermentation vessel culture medium monitoring apparatus of claim 4, wherein, The analysis detector (40) and the feeding mechanism (50) are respectively electrically connected to a control system (60), and the control system (60) is used for controlling the feeding mechanism (50) to supplement glucose into the fermentation container (10) according to the detection result of the analysis detector (40). The feeding mechanism (50) comprises a feeding buffer (51) used for containing a glucose supplement solution, a metering part (52) connected to the feeding buffer (51), and a feeding driving part (53) connected between the feeding buffer (51) and the fermentation container (10) and electrically connected to the control system (60). ​ ​ 7. An automated sample addition fermentation system characterized by, ​ ​ ​ ​ ​ 8. The automated sample addition fermentation system of claim 7, wherein, ​ ​ ​ ​ 9. The automated sample addition fermentation system of claim 8, wherein, The number of the fermentation containers (10) is multiple, the number of the sampling mechanisms (20) is multiple, the number of the metering members (52) is multiple, the number of the feeding driving members (53) is multiple, one-to-one correspondence of multiple sampling mechanisms (20) is connected to multiple fermentation containers (10) respectively, multiple sampling mechanisms (20) are connected to the analysis and detection instrument (40) respectively, one-to-one correspondence of multiple feeding driving members (53) is connected to multiple fermentation containers (10) respectively, one-to-one correspondence of multiple metering members (52) is connected to multiple feeding driving members (53) respectively, and multiple metering members (52) are connected to the feeding buffer (51).

10. The automated sample addition fermentation system of claim 7, wherein, The analysis and detection instrument (40) and the control system (60) are arranged in a clean environment.

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