Purification device for preparing biological polysaccharide

Abstract

The utility model relates to the field of bioengineering, and discloses a purification device for preparing biological polysaccharide, which comprises a filter cartridge and a purification box, the upper surface of the filter cartridge is fixedly connected with a material receiving cover, the upper surface of the filter cartridge is provided with a motor, the output end of the motor is fixedly connected with a rotating rod, the outer wall of the rotating rod is fixedly connected with stirring blades, and the stirring blades are fixedly connected with the purification box. An activated carbon filter plate is fixedly connected to the inner wall of the filter cartridge, a connecting ring is arranged in the filter cartridge, a clamping rod is fixedly connected to the outer wall of the connecting ring, a clamping assembly is arranged on the outer wall of the filter cartridge and used for fixing the clamping rod, and a sliding assembly is arranged in the connecting ring. The sliding assembly is used for assisting the connecting ring to move up and down. According to the utility model, the cleaning of the activated carbon filter plate is realized, so that the mode that the filter plate needs to be detached and cleaned in the traditional purification device is avoided, the workload of operators is greatly reduced, and the waste of raw material liquid is effectively reduced through the arrangement of the scraper.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of bioengineering, especially to a purification device for preparing biological polysaccharide. BACKGROUND

[0002] Biological polysaccharide is a kind of biological macromolecule with important physiological functions and extensive application value. In the pharmaceutical field, it can be used as a drug carrier, an immunomodulator, an anticoagulant, etc., providing a key material basis for the research and development of new drugs and disease treatment. In its preparation process, the purity of raw materials and intermediate products needs to be ensured to ensure the quality and performance of the final product. Therefore, in order to ensure the quality of biological polysaccharide, a purification device for preparing biological polysaccharide has emerged.

[0003] The purification device for biological polysaccharide generally includes filtering, precipitation, ion exchange chromatography, gel filtration chromatography, dialysis, etc. The filtering operation is the most important step in the preparation of biological polysaccharide, which is generally composed of multiple layers of filter screens, filter membranes or filter media, and the impurities are separated from the solution or mixture by physical interception, adsorption, etc.

[0004] However, most of the existing biological polysaccharide purification devices focus on the optimization of filtering function in the design, while ignoring the cleaning process. Since there is a lack of efficient cleaning mechanism for the filtering mechanism in the existing device, there is no suitable cleaning interface, automatic cleaning device or easy-to-disassemble structure, which leads to the need for a large amount of time and effort for the staff when cleaning the filtering mechanism, and even the entire purification device may need to be partially disassembled, thereby increasing the maintenance cost and downtime, and adversely affecting the continuous production process of biological polysaccharide. SUMMARY

[0005] In order to make up for the above shortcomings, the utility model provides a purification device for preparing biological polysaccharide, which aims to improve the problem that the existing biological polysaccharide purification device ignores the cleaning process, thereby requiring a large amount of time and effort for the staff.

[0006] In order to achieve the above object, the utility model provides the following technical scheme: A kind of purification device for preparing biological polysaccharide, including filter cartridge and purification tank, the filter cartridge upper surface is fixedly connected with receiving cover, the filter cartridge upper surface is provided with motor, the motor output end is fixedly connected with rotating rod, the rotating rod outer wall is fixedly connected with stirring blade, the filter cartridge inner wall is fixedly connected with activated carbon filter plate, the filter cartridge is internally provided with connecting ring, the connecting ring outer wall is fixedly connected with clamping rod, the filter cartridge outer wall is provided with clamping assembly, the clamping assembly is used to fixed clamping rod, the connecting ring is internally provided with sliding assembly, the sliding assembly is used to assist the up and down movement of connecting ring, the clamping assembly includes clamping barrel, the clamping barrel inside is fixedly connected with spring, one end of the spring is fixedly connected with clamping block, the clamping block outer wall one side is fixedly connected with pull block, the clamping barrel inside is provided with sliding groove one.

[0007] Further, the sliding assembly includes guide groove, the guide groove is opened in the connecting ring inside, the filter cartridge inside is fixedly connected with guide rod, the guide rod outer wall is slidably connected in the guide groove inner wall.

[0008] Further, the rotating rod outer wall is fixedly connected with connecting rod, the connecting rod outer wall is slidably connected with sleeve rod, the sleeve rod outer wall one side is fixedly connected with scraper, the connecting rod upper surface is fixedly connected with slide nail.

[0009] Further, the sleeve rod inside is provided with sliding groove two, the slide nail outer wall is slidably connected in the sliding groove two inner wall.

[0010] Further, the clamping rod inside is provided with clamping groove, and the clamping groove is used to be clamped with clamping block.

[0011] Further, the filter cartridge lower surface is fixedly connected with flow guide pipe, and the flow guide pipe outer wall is provided with water pump.

[0012] Further, the purification tank inside is fixedly connected with purification cartridge, and the purification cartridge inside is provided with chromatography column and gel column.

[0013] Further, the purification tank inside is slidably connected with dialysis box, and the dialysis box is used to further purify polysaccharide.

[0014] The utility model has the advantages of:

[0015] 1. In the utility model, through pulling the connecting ring upwards, the connecting ring can slide upwards along the guide rod, thereby realizing the upward movement of the clamping rod outside, and the clamping rod will slide along the sliding groove inside the clamping sleeve, when the clamping rod slides, the clamping block will be extruded, thereby realizing the movement of the clamping block into the clamping sleeve, and the spring will be extruded and contracted by the clamping block, until the clamping groove slides to the position opposite to the clamping block, and then through the elastic action of spring recovery, the clamping of the clamping block and the clamping groove is realized, thereby realizing the fixation of the connecting ring, and then through the slot opened by the upward movement of the connecting ring, the cleaning of the activated carbon filter plate is realized, thereby avoiding the mode of disassembling and cleaning the filter plate of the traditional purification device, and greatly reducing the workload of the operator.

[0016] 2. In the utility model, the rotating rod is driven by the motor, thereby the plurality of stirring blades outside can be driven to rotate, thereby realizing the full contact of the raw material and the precipitating agent, when the rotating rod rotates, the connecting rod will also rotate, thereby the sleeve rod outside the connecting rod can extend outward under the limiting of the slide pin and the sliding groove, thereby realizing the full contact of the inner wall of the scraper filter cylinder, and then through the driving rotation of the connecting rod, the scraper can scrape off the raw material liquid on the inner wall of the filter cylinder, thereby effectively reducing the waste of the raw material liquid. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 A three-dimensional structure schematic view of the purification device for preparing biological polysaccharide is provided for the utility model;

[0018] Figure 2 A filter cylinder structure schematic view of the purification device for preparing biological polysaccharide is provided for the utility model;

[0019] Figure 3 A connecting ring structure schematic view of the purification device for preparing biological polysaccharide is provided for the utility model;

[0020] Figure 4 A clamping sleeve structure schematic view of the purification device for preparing biological polysaccharide is provided for the utility model;

[0021] Figure 5 A connecting box structure schematic view of the purification device for preparing biological polysaccharide is provided for the utility model;

[0022] Figure 6 A scraper structure schematic view of the purification device for preparing biological polysaccharide is provided for the utility model.

[0023] LEGEND:

[0024] 1, filter cartridge; 2, motor; 3, receiving cover; 4, rotating rod; 5, activated carbon filter plate; 6, connecting ring; 7, guide rod; 8, guide groove; 9, clamping rod; 10, clamping groove; 11, stirring blade; 12, clamping sleeve; 13, sliding groove I; 14, clamping block; 15, spring; 16, pulling block; 17, purification tank; 18, purification cartridge; 19, dialysis box; 20, flow guide pipe; 21, water pump; 22, scraper; 23, connecting rod; 24, sleeve rod; 25, sliding groove II; 26, sliding pin. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0026] Reference Figure 1 - Figure 2The utility model provides a kind of embodiment: a kind of purification device for preparing biological polysaccharide, including filter cartridge 1 and purification tank 17, filter cartridge 1 upper surface is fixedly connected with material receiving cover 3, first raw material liquid is passed through material receiving cover 3 and enters filter cartridge 1, the filter screen arranged in filter cartridge 1 inside will intercept large particle impurities in raw material liquid, the activated carbon filter plate 5 arranged in filter cartridge 1 will carry out preliminary filtration to the raw material entering, activated carbon carries out adsorption filtration to small molecule impurities in raw material liquid, after filtering, according to certain proportion, slowly add precipitant to filter cartridge 1, then start motor 2, motor 2 output end is fixedly connected with rotating rod 4, rotating rod 4 outer wall is fixedly connected with stirring blade 11, motor 2 can drive the stirring blade 11 of rotating rod 4 outer wall and stir raw material, so that raw material and precipitant are fully contacted, to realize the separation of polysaccharide and impurity, polysaccharide solution separated passes through the flow guide pipe 20 under filter cartridge 1 and flows out, and then pass through water pump 21 and provide power, polysaccharide solution can be guided into purification cartridge 18, filter cartridge 1 upper surface is provided with motor 2, filter cartridge 1 inner wall is fixedly connected with activated carbon filter plate 5, filter cartridge 1 inside is provided with connecting ring 6, connecting ring 6 outer wall is fixedly connected with clamping rod 9, filter cartridge 1 outer wall is provided with clamping assembly, and clamping assembly is used to fixed clamping rod 9, connecting ring 6 inside is provided with sliding assembly, and sliding assembly is used to assist the up-and-down movement of connecting ring 6, filter cartridge 1 lower surface is fixedly connected with flow guide pipe 20, and flow guide pipe 20 outer wall is provided with water pump 21, purification tank 17 inside is fixedly connected with purification cartridge 18, and purification cartridge 18 inside is provided with chromatography column and gel column, by the fact that purification cartridge 18 inside is provided with chromatography column and gel column, the impurity that the difference of molecular weight and polysaccharide is effectively removed, to obtain polysaccharide component purified further, the impurity that the difference of molecular weight and polysaccharide in polysaccharide solution is removed, polysaccharide solution purified through previous step is loaded into dialysis cassette 19, since there is concentration gradient inside and outside dialysis cassette 19, small molecule impurities will diffuse to dialysate through semi-permeable membrane, and polysaccharide molecules are retained in dialysis cassette 19, to realize the further purification of polysaccharide, purification tank 17 inside is slidably connected with dialysis cassette 19, and dialysis cassette 19 is used to further purify polysaccharide.

[0027] Refer to Figure 2 Figure 4 ​The clamping assembly includes a clamping tube 12, a spring 15 is fixedly connected to the inside of the clamping tube 12, one end of the spring 15 is fixedly connected to a clamping block 14, and one side of the outer wall of the clamping block 14 is fixedly connected to a pull block 16. A slide groove 13 is provided inside the clamping tube 12. When the activated carbon filter plate 5 needs to be cleaned, the connecting ring 6 can be pulled upward first so that the connecting ring 6 can slide upward along the guide rod 7. At the same time, the connecting ring 6 will also drive the clamping rod 9 on its outer side to move upward. The guide rod 7 is fixed to the inside of the filter cartridge 1, and a guide groove 8 is provided inside the connecting ring 6. The outer wall of the guide rod 7 is slidably connected to the inner wall of the guide groove 8, which provides a guiding effect for the up and down sliding of the connecting ring 6. When the clamping rod 9 is in the When moving upward, it will enter the inside of the clamping tube 12, and the clamping rod 9 can slide along the internal slide groove 13 of the clamping tube 12. At the same time, the clamping rod 9 will squeeze the clamping block 14 when sliding, so that the clamping block 14 will move into the clamping tube 12. At the same time, the spring 15 will also be squeezed and contracted by the clamping block 14 until the clamping groove 10 inside the clamping rod 9 slides to a position facing the clamping block 14. The elastic action of the spring 15 restores the clamping block 14, so that the clamping block 14 can be completely clamped in the inside of the clamping groove 10, thereby achieving the fixation of the connecting ring 6, and then the cleaning of the activated carbon filter plate 5 is achieved by moving the connecting ring 6 upward to open the notch. The sliding assembly includes a guide groove 8, which is opened inside the connecting ring 6.

[0028] Reference Figure 5 - Figure 6 When the rotating rod 4 rotates, the connecting rod 23 on its outer wall is driven to rotate. Since the connecting rod 23 and the sleeve rod 24 are slidably connected, and the sliding nail 26 on the connecting rod 23 slides in the slide groove 25 of the sleeve rod 24, the centrifugal force generated by the rotation of the rotating rod 4 will drive the connecting rod 23 to rotate, thereby causing the sleeve rod 24 on the outer wall of the connecting rod 23 to extend outward, thereby realizing that the scraper 22 on the outer wall of the sleeve rod 24 can fully contact the inner wall of the filter drum 1, and the scraper 22 can clean the inner wall of the filter drum 1 through the driven rotation of the connecting rod 23, effectively reducing the waste of raw material liquid.

[0029] Working principle: when using the device, first, the raw material liquid enters the filter cartridge 1 through the receiving cover 3, the filter screen arranged inside the filter cartridge 1 can intercept the large particle impurities in the raw material liquid, and the activated carbon filter plate 5 arranged in the filter cartridge 1 can preliminarily filter the entering raw material, the activated carbon can adsorb and filter the small molecular impurities in the raw material liquid, after the filtration is completed, the precipitating agent is slowly added into the filter cartridge 1 according to a certain proportion, and then the motor 2 is started, the stirring blade 11 on the outer wall of the rotating rod 4 can be driven to stir the raw material, so that the polysaccharide and the impurities are separated, the separated polysaccharide solution flows out through the flow guide pipe 20 below the filter cartridge 1, and then the polysaccharide solution is guided into the purification cartridge 18 through the water pump 21, the chromatography column and the gel column arranged inside the purification cartridge 18 can effectively remove the impurities with large molecular weight difference from the polysaccharide, so that the polysaccharide component is further purified, the impurities with large molecular weight difference from the polysaccharide in the polysaccharide solution are removed, the polysaccharide solution purified through the above steps is loaded into the dialysis box 19, since there is a concentration gradient between the inside and outside of the dialysis box 19, the small molecular impurities diffuse into the dialysis liquid through the semi-permeable membrane, and the polysaccharide molecules are retained in the dialysis box 19, so that the polysaccharide is further purified;

[0030] When the activated carbon filter plate 5 needs to be cleaned, the connecting ring 6 can be pulled upwards first, so that the connecting ring 6 can slide upwards along the guide rod 7, and the connecting ring 6 can also drive the clamping rod 9 outside to move upwards, when the clamping rod 9 moves upwards, it enters the inside of the clamping sleeve 12, and the clamping rod 9 can slide along the sliding groove one 13 in the inside of the clamping sleeve 12, and the clamping rod 9 can extrude the clamping block 14 when sliding, so that the clamping block 14 moves into the clamping sleeve 12, and the spring 15 is also extruded and contracted by the clamping block 14, until the clamping groove 10 in the clamping rod 9 slides to the position opposite to the clamping block 14, the elastic effect of the spring 15 after restoration makes the clamping block 14 completely clamped in the clamping groove 10, so that the fixing of the connecting ring 6 is realized, and then the slot opened by the upward movement of the connecting ring 6 realizes the cleaning of the activated carbon filter plate 5;

[0031] When the rotating rod 4 rotates, the connecting rod 23 on the outer wall of the rotating rod 4 rotates, so that the sleeve rod 24 on the outer wall of the connecting rod 23 extends outward under the driving rotation of the rotating rod 4, so that the scraper 22 on the outer wall of the sleeve rod 24 can fully contact with the inner wall of the filter cartridge 1, and the scraper 22 can clean the inner wall of the filter cartridge 1 under the driving rotation of the connecting rod 23, so as to effectively reduce the waste of the raw material liquid.

[0032] Finally, it should be noted that: the above only for the preferred embodiments of the present application, and is not intended to limit the present application, although the foregoing embodiments of the present application has been described in detail, for the skilled in the art, it still can be modified, or for part of the technical features of the equivalent replacement, the spirit and principles of the present application, made any modification, equivalent replacement, improvement, etc., should be included within the scope of the present application.

Claims

1. A purification device for the preparation of biological polysaccharides comprising a filter cartridge (1) and a purification tank (17), characterized in that: The upper surface of the filter cartridge (1) is fixedly connected with a receiving cover (3), the upper surface of the filter cartridge (1) is provided with a motor (2), the output end of the motor (2) is fixedly connected with a rotating rod (4), the outer wall of the rotating rod (4) is fixedly connected with a stirring blade (11), the inner wall of the filter cartridge (1) is fixedly connected with an activated carbon filter plate (5), the inside of the filter cartridge (1) is provided with a connecting ring (6), the outer wall of the connecting ring (6) is fixedly connected with a clamping rod (9), the outer wall of the filter cartridge (1) is provided with a clamping assembly, the clamping assembly is used for fixing the clamping rod (9), the inside of the connecting ring (6) is provided with a sliding assembly, and the sliding assembly is used for assisting the up-down movement of the connecting ring (6). The clamping assembly comprises a clamping sleeve (12), the inside of the clamping sleeve (12) is fixedly connected with a spring (15), one end of the spring (15) is fixedly connected with a clamping block (14), one side of the outer wall of the clamping block (14) is fixedly connected with a pulling block (16), and a sliding groove one (13) is formed in the inside of the clamping sleeve (12).

2. A purification apparatus for the preparation of biological polysaccharides according to claim 1, characterized in that: The sliding assembly comprises a guide groove (8), the guide groove (8) is formed in the inside of the connecting ring (6), and the inside of the filter cartridge (1) is fixedly connected with a guide rod (7).

3. A purification unit for the preparation of biological polysaccharides according to claim 1, characterized in that: The outer wall of the rotating rod (4) is fixedly connected with a connecting rod (23), the outer wall of the connecting rod (23) is slidably connected with a sleeve rod (24), one side of the outer wall of the sleeve rod (24) is fixedly connected with a scraper (22), and the upper surface of the connecting rod (23) is fixedly connected with a sliding nail (26).

4. A purification apparatus for the preparation of biological polysaccharides according to claim 3, characterized in that: The inside of the sleeve rod (24) is provided with a sliding groove two (25), and the outer wall of the sliding nail (26) is slidably connected to the inner wall of the sliding groove two (25).

5. A purification unit for the preparation of biological polysaccharides according to claim 1, characterized in that: The inside of the clamping rod (9) is provided with a clamping groove (10), and the clamping groove (10) is used for clamping the clamping block (14).

6. The purification device for preparing biopolysaccharides according to claim 1, characterized in that: The lower surface of the filter cartridge (1) is fixedly connected with a flow guide pipe (20), and the outer wall of the flow guide pipe (20) is provided with a water pump (21).

7. A purification unit for the preparation of biological polysaccharides according to claim 1, characterized in that: The inside of the purification box (17) is fixedly connected with a purification cylinder (18), and the inside of the purification cylinder (18) is provided with a chromatography column and a gel column.

8. A purification unit for the preparation of biological polysaccharides according to claim 1, characterized in that: The inside of the purification box (17) is slidably connected with a dialysis box (19), and the dialysis box (19) is used for further purifying polysaccharides.

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