Reaction tube fixing device

Abstract

The utility model belongs to the technical field of chemistry, and provides a reaction tube fixing device. The reaction tube fixing device comprises a fixing base plate and a fixing part, wherein a plurality of single tube holes and a groove positioned between at least two single tube holes are formed in the surface of the fixing base plate; the single tube hole is used for placing a reaction tube; the fixing part is detachably connected with the fixing base plate, the fixing part comprises a fixing plate, the fixing plate comprises a first end face and a second end face which are opposite to each other, the first end face is embedded into the groove, the second end face is provided with a limiting groove, and the limiting groove is used for limiting the connecting strip between the two adjacent reaction tubes. The reaction tubes and the connecting strips are fixed through cooperation of the grooves, the fixing plates and the limiting grooves, the problem that in the experiment process, due to the fact that the row tubes cannot be fixed, the row tubes deform, and the experiment fails is solved, and the reaction tubes and the connecting strips can be firmly placed in the use process and taken out conveniently and smoothly.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of chemistry, and provides a reaction tube fixing device. BACKGROUND

[0002] In a fluorescent quantitative polymerase chain reaction (PCR) test, an eight-row PCR tube is the most commonly used reaction tube, and the fluorescent quantitative PCR experiment is currently widely and commonly used in the fields of biology and medicine.

[0003] A commonly used eight-row PCR tube is usually composed of eight tube bodies through a middle small connecting strip, and the material is relatively soft, and therefore the deformation of one eight-row PCR tube is relatively large. Usually in the test process, the experimenter fixes the eight-row PCR tube by using a PCR module, and if the eight-row PCR tube is not fixed well during centrifugation or heating, the tube body will be subjected to extrusion or uneven pressure, resulting in deformation, so that part of the tube body cannot be closely combined with the module hole wall, leading to abnormal temperature control of the reagent in the tube during the experiment process, and experiment failure.

[0004] The existing PCR module only has several single holes for fixing a plurality of eight-row PCR tubes, and the eight-row PCR tube can still be loose during high temperature and thermal cycling, and the eight-row PCR tube can fall off from the module, leading to experiment failure. SUMMARY

[0005] The application embodiment provides the technical problem to be solved by the application, that is, fixing the row tube through a simple structure without affecting normal placement or liquid suction operation, and ensuring that the row tube will not cause experiment failure due to deformation during the experiment process.

[0006] To solve the above problems, the application provides a reaction tube fixing device, which comprises a fixing base plate and a fixing piece. The surface of the fixing base plate is provided with a plurality of single tube holes and a groove between at least two single tube holes; the single tube hole is used for placing a reaction tube; the fixing piece is detachably connected with the fixing base plate, and the fixing piece comprises a fixing plate, the fixing plate comprises opposite first and second end faces, the first end face is embedded in the groove, and the second end face is provided with a limiting groove for limiting the connecting strip between the adjacent two reaction tubes.

[0007] In an embodiment, the opening diameter of the limiting groove is smaller than the bottom diameter of the limiting groove.

[0008] In an embodiment, the opening diameter of the limiting groove is greater than the width of the connecting strip, and the difference between the opening diameter of the limiting groove and the width of the connecting strip is less than a preset value.

[0009] In an embodiment, the side wall of the limiting groove is a circular arc surface, and when the reaction tube is placed in the single-tube hole, the center of the circular arc surface is higher than the position of the connecting strip.

[0010] In an embodiment, when the reaction tube is placed in the single-tube hole, the first end surface of the fixing plate is lower than the highest position of the reaction tube.

[0011] In an embodiment, the fixing member further comprises a first fixing part and a second fixing part, and the first fixing part and the second fixing part are fixedly connected to the fixing plate at both ends in the length direction of the fixing plate.

[0012] The fixing base plate comprises opposite first and second side surfaces, the first fixing part is used for detachable connection with the first side surface of the fixing base plate, and the second fixing part is used for detachable connection with the second side surface of the fixing base plate.

[0013] In an embodiment, the first and second side surfaces have first fixing holes, the first and second fixing parts have second fixing holes, and the first and second fixing holes are detachably connected through fasteners.

[0014] In an embodiment, the plurality of single-tube holes are arranged in an array, and the groove has a plurality of grooves extending in the same direction.

[0015] In an embodiment, the groove has three grooves, and the first and second grooves are symmetrical to the third groove.

[0016] In an embodiment, the surface of the fixing base plate further comprises a plurality of weight-removing holes, and the plurality of weight-removing holes are arranged at intervals with the plurality of single-tube holes.

[0017] In an embodiment, the fixing base plate comprises a plurality of counterbores on the side surface, and the counterbores are used for fixing the fixing base plate to the test bench.

[0018] In an embodiment, the reaction tube fixing device further comprises a row pipe, and the row pipe comprises a plurality of reaction tubes, and the plurality of reaction tubes are connected in sequence through the connecting strip.

[0019] In an embodiment, the row pipe is an a-row PCR pipe, the fixing base plate has a×b single-tube holes, the a×b single-tube holes are arranged in a rows and b columns, and the groove extends in the column direction.

[0020] In an embodiment, the row pipe is an eight-row PCR pipe, the fixing base plate has 96 single-tube holes, the 96 single-tube holes are arranged in 8 rows and 12 columns, and the groove extends in the column direction.

[0021] The technical scheme provided by the above embodiments of the application is characterized in that a plurality of single-tube holes and a groove between at least two single-tube holes for placing reaction tubes are arranged on the surface of a fixed base plate; a first end surface of the fixed plate is embedded in the groove, and a second end surface is provided with a limiting groove; and a device for limiting the connecting strip between adjacent two reaction tubes is used to fix the parallel-tube device. The problem that the parallel-tube device is not fixed well in experiments, the tube body is squeezed or unevenly pressed, and deformation occurs to cause experimental failure is solved, so that the reaction tube and the connecting strip can be placed firmly and conveniently and smoothly taken out during use. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical scheme of the embodiments of the application, the drawings used in the embodiments of the application will be briefly introduced.

[0023] Figure 1 The assembly drawing of the reaction tube fixing device provided by the embodiments of the application is shown in the figure.

[0024] Figure 2 The schematic diagram of the fixing member device provided by the embodiments of the application is shown in the figure.

[0025] Figure 3 The schematic diagram of the fixed base plate device provided by the embodiments of the application is shown in the figure.

[0026] Figure 4 The schematic diagram of the parallel-tube device provided by the embodiments of the application is shown in the figure.

[0027] Among them, the above-mentioned drawings include the following marks:

[0028] 1-fixed base plate; 10-single-tube hole; 11-groove; 12-first side surface; 13-second side surface, 14-first fixing hole, 15-weight hole; 16-counterbore hole; 17-first groove; 18-second groove; 19-third groove;

[0029] 2-fixing member; 20-fixed plate, 21-first end surface; 22-second end surface; 23-limiting groove; 24-first fixing part; 25-second fixing part; 26-second fixing hole;

[0030] 3-parallel-tube device; 30-connecting strip; 31-reaction tube. DETAILED DESCRIPTION

[0031] The technical scheme in the embodiments of the application will be described below with reference to the drawings in the embodiments of the application.

[0032] Similar reference numerals and letters denote similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings. At the same time, in the description of this application, the terms "first", "second", etc. are only used to distinguish the description and should not be understood as indicating or implying relative importance.

[0033] Figure 1 This is an assembly diagram of the reaction tube 31 fixing device provided in the embodiment of the present application. Figure 1 As shown, the present application provides a device for fixing a reaction tube 31, comprising: a fixing base plate 1 and a fixing member 2. The surface of the fixing base plate 1 is provided with a plurality of single-tube holes 10 and a groove 11 located between at least two of the single-tube holes 10; the single-tube holes 10 are used to place the reaction tubes 31; the shape of the single-tube holes 10 is close to the body of the reaction tube 31, and the inner wall of the single-tube hole 10 can be in close contact with the outer wall of the reaction tube 31, thereby ensuring efficient heat conduction. The fixing member 2 is made by cutting and bending a stainless steel sheet metal and is detachably connected to the fixing base plate 1 via the groove 11.

[0034] Figure 2 This is a structural diagram of the fixing member 2 provided in the embodiment of the present application, combined with Figure 2 As shown, the fixing member 2 includes a fixing plate 20, and the fixing plate 20 includes a first end face 21 and a second end face 22 relative to each other. The first end face 21 is embedded in the groove 11, and the second end face 22 is provided with a limiting groove 23, which is used to limit the connecting strip 30 between two adjacent reaction tubes 31.

[0035] The connecting bar 30 is placed parallel to the y-axis in the fixing device of the reaction tube 31 , the groove 11 is arranged parallel to the x-axis in the fixing device of the reaction tube 31 , and the groove 11 and the connecting bar 30 are arranged perpendicularly and crosswise in the xy plane.

[0036] The technical solution provided by the above-mentioned embodiment of the present application fixes the strip tubes 3 by fixing the base plate 1 and the fixing member 2, so that during the experiment, the experimenter only needs to place the first end face 21 of the fixing plate 20 in the groove 11, and then place the reaction tube 31 connected by the connecting bar 30 into the corresponding single tube hole 10 of the fixing base plate 1 according to experimental needs, and press down slightly to ensure that the reaction tube 31 is completely inserted and that the limiting groove 23 completely clamps the connecting bar 30, thereby completing the fixation of the reaction tube 31 and the connecting bar 30, eliminating the problem of experimental failure caused by deformation of the reaction tube 31 due to the inability to fix the strip tube 3. At the same time, this structure does not affect the normal removal of the reaction tube 31 and the connecting bar 30.

[0037] In one embodiment, if Figure 2As shown, the opening diameter of the limiting groove 23 is smaller than the bottom diameter of the limiting groove 23, and the opening height of the limiting groove 23 is the same as the height of the connecting strip 30 after the reaction tube 31 is placed on the fixed base plate 1, so that the connecting strip 30 can be clamped at the opening of the limiting groove 23.

[0038] In an embodiment, the opening diameter of the limiting groove 23 is greater than the width of the connecting strip 30, so that the connecting strip 30 can be smoothly placed at the opening of the limiting groove 23, and the difference between the opening diameter of the limiting groove 23 and the width of the connecting strip 30 is less than a preset value, so that the connecting strip 30 can be clamped with the limiting groove 23.

[0039] The preset value ensures that the connecting strip 30 of the reaction tube 31 can be slightly clamped but not too tight to cause inconvenience in placing and taking out, and avoids that the connecting strip 30 is clamped too tightly with the limiting groove 23, so that the reaction tube 31 produces a strong recoil when taken out, causing the amplification reagent to splash and the instrument surface to be contaminated.

[0040] In an embodiment, the side wall of the limiting groove 23 is two circular arc surfaces, the circular arc surfaces are smooth in transition and have a larger upper opening, and when the reaction tube 31 is placed, the connecting strip 30 can be smoothly placed between the two circular arcs. When the reaction tube 31 is placed in the single tube hole 10, the center of the circular arc surface is higher than the position of the connecting strip 30, which ensures that the connecting strip 30 can be clamped after the reaction tube 31 is placed.

[0041] The minimum distance between the two circular arcs is just greater than the width of the connecting strip 30 of the reaction tube 31, and the difference between the minimum distance between the two circular arcs and the width of the connecting strip 30 is less than a preset value, which ensures that the connecting strip 30 of the reaction tube 31 can be slightly clamped but not too tight to cause inconvenience in placing and taking out.

[0042] In an embodiment, when the reaction tube 31 is placed in the single tube hole 10, the first end surface 21 is flush, and the first end surface 21 of the fixed plate 20 is lower than the highest position of the reaction tube 31, which prevents interference with structures such as a pipette during the test, causing errors in the experiment or leading to experimental failure.

[0043] Figure 3 A fixed base plate 1 device schematic diagram provided in an embodiment of the present application is shown in FIG. 1. Figures 2-3 As shown, in an embodiment, the fixing member 2 further includes a first fixing portion 24 and a second fixing portion 25, the first fixing portion 24 and the second fixing portion 25 are located at both ends of the fixed plate 20 in the length direction, i.e., the fixed plate 20 is arranged in parallel with the X-axis, the first fixing portion 24 and the second fixing portion 25 are arranged in parallel with the Y-axis, and both ends of the fixed plate 20 are fixedly connected with the first fixing portion 24 and the second fixing portion 25, respectively.

[0044] The fixed substrate 1 includes a first side surface 12 and a second side surface 13 arranged opposite to each other. The first side surface 12 and the second side surface 13 are arranged parallel to the Y axis. The first fixing portion 24 is used to be detachably connected to the first side surface 12 of the fixed substrate 1; the second fixing portion 25 is used to be detachably connected to the second side surface 13 of the fixed substrate 1.

[0045] In one embodiment, the first side surface 12 and the second side surface 13 have a first fixing hole 14 , the first fixing portion 24 and the second fixing portion 25 have a second fixing hole 26 , and the first fixing hole 14 and the second fixing hole 26 are detachably connected via fasteners.

[0046] In one embodiment, the plurality of single tube holes 10 are arranged in an array, and there are a plurality of grooves 11 , and the plurality of grooves 11 extend in the same direction.

[0047] The connecting bar 30 is placed parallel to the y-axis in the fixing device of the reaction tube 31 , the groove 11 is arranged parallel to the x-axis in the fixing device of the reaction tube 31 , and the groove 11 and the connecting bar 30 are arranged perpendicularly and crosswise in the xy plane.

[0048] like Figure 3 As shown, in one embodiment, three grooves 11 are provided: a first groove 17, a second groove 18, and a third groove 19, arranged parallel to the X-axis in the fixed base plate 1. The third groove 19 is located at the axial midline of the fixed base plate 1. The first groove 17 and the second groove 18 are located on either side of the midline, near the single tube holes 10 at the two axial ends of the fixed base plate 1. The first groove 17 and the second groove 18 are symmetrical with respect to the third groove 19. The reaction tubes 31 connected by the connecting strips 30 are placed parallel to the Y-axis in the fixed base plate 1. The three grooves 11 respectively capture the connecting strips 30 at the middle and at the ends of the reaction tubes 31, securing the connecting strips 30 and preventing deformation at one end or the middle of the tube strips 3 during experiments due to forces such as pipetting. Furthermore, the reaction tubes 31 and connecting strips 30 can be removed without significant resistance and without additional manipulation. The entire structure is securely placed and easily and smoothly removed during use.

[0049] In one embodiment, the surface of the fixed substrate 1 also includes a plurality of de-weighting holes 15, which are arranged at intervals with the plurality of single-tube holes 10 to reduce the overall weight of the fixed substrate 1 and increase the heating and cooling rate when controlling the temperature of the fixed substrate 1.

[0050] In one embodiment, the fixed base plate 1 includes a plurality of countersunk holes 16 on the side surface, and the countersunk holes 16 are used to fix the fixed base plate 1 to the test bench.

[0051] Figure 4 This is a schematic diagram of the arrangement of the tubes 3 provided in the embodiment of the present application. Figures 2-3As shown in an embodiment, the reaction tube 31 fixing device further comprises: a row of tubes 3, the row of tubes 3 comprising a plurality of reaction tubes 31, the plurality of reaction tubes 31 being connected in sequence by connecting strips 30.

[0052] In combination Figure 1 As shown in an embodiment, the row of tubes 3 is an eight-row PCR tube, and the fixing base plate 1 has 96 single-tube holes 10, which are arranged in 8 rows (the rows being parallel to the Y axis) and 12 columns (the columns being parallel to the X axis), and the grooves 11 extend in the column direction (parallel to the X axis).

[0053] In an embodiment, the row of tubes 3 is an eight-row PCR tube (i.e., a can be eight), and the fixing base plate 1 has 96 single-tube holes 10, which are arranged in 8 rows (the rows being parallel to the Y axis) and 12 columns (the columns being parallel to the X axis), and the grooves 11 extend in the column direction (parallel to the X axis).

[0054] The above embodiments are only used to illustrate the technical solutions of the present application and are not used to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application. The above embodiments are only used to illustrate the technical solutions of the present application and are not used to limit the present application. For those skilled in the art, the present application can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A reaction tube fixing device characterized by comprising: The reaction tube fixing device comprises: a fixing base plate, a surface of the fixing base plate is provided with a plurality of single tube holes and a groove between at least two single tube holes; the single tube hole is used for placing a reaction tube; a fixing piece, the fixing piece is detachably connected with the fixing base plate, the fixing piece comprises a fixing plate, the fixing plate comprises opposite first and second end faces, the first end face is embedded in the groove, and the second end face is provided with a limiting groove for limiting a connecting strip between adjacent two reaction tubes.

2. The reaction tube holder of claim 1, wherein The opening diameter of the limiting groove is smaller than the bottom diameter of the limiting groove.

3. The reaction tube holder of claim 1, wherein, The opening diameter of the limiting groove is greater than the width of the connecting strip, and the difference between the opening diameter of the limiting groove and the width of the connecting strip is less than a preset value.

4. The reaction tube fixing device according to claim 1, characterized in that: The side wall of the limiting groove is two circular arc surfaces, and when the reaction tube is placed in the single tube hole, the center position of the circular arc surface is higher than the position of the connecting strip.

5. The reaction tube holder of claim 1, wherein When the reaction tube is placed in the single tube hole, the first end face of the fixing plate is lower than the highest position of the reaction tube.

6. The reaction tube holder of claim 1, wherein The fixing piece further comprises a first fixing part and a second fixing part, and the first fixing part and the second fixing part are located at both ends of the length direction of the fixing plate and are fixedly connected with the fixing plate. The fixing base plate comprises opposite first and second side faces, the first fixing part is used for detachable connection with the first side face of the fixing base plate, and the second fixing part is used for detachable connection with the second side face of the fixing base plate.

7. The reaction tube holder of claim 6, wherein, The first and second side faces have first fixing holes, the first and second fixing parts have second fixing holes, and the first fixing holes and the second fixing holes are detachably connected through fasteners.

8. The reaction tube holder of claim 1, wherein, The plurality of single tube holes are arranged in an array, and the groove has a plurality of grooves extending in the same direction.

9. The reaction tube holder of claim 1, wherein, The groove has three grooves, and the first and second grooves are symmetrical to the third groove.

10. The reaction tube holder of claim 1, wherein, The surface of the fixing base plate further comprises a plurality of weight removal holes, and the plurality of weight removal holes are arranged at intervals with the plurality of single tube holes.

11. The reaction tube holder of claim 1, wherein, The fixing base plate comprises a plurality of counterbores on the side face, and the counterbores are used for fixing the fixing base plate with a test bench.

12. The reaction tube holder of claim 1, wherein, Further comprising: a row of tubes, the row of tubes comprises a plurality of reaction tubes, and the plurality of reaction tubes are connected in sequence through connecting strips.

13. The reaction tube holder of claim 12, wherein, The row of tubes is an a row of PCR tubes, the fixing base plate has a×b single tube holes, the a×b single tube holes are arranged in a row of a and a column of b, and the groove extends in the column direction.

14. The reaction tube holder of claim 13, wherein, The row of tubes is an eight row of PCR tubes, the fixing base plate has 96 single tube holes, the 96 single tube holes are arranged in 8 rows and 12 columns, and the groove extends in the column direction.

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