Fluid detection system and gene sequencing kit

Abstract

The utility model relates to the field of gene detection, aims to solve the problem of low detection efficiency of fluid system detection in the prior art, and provides a fluid detection system and a gene sequencing kit. The fluid detection system comprises a test tube support assembly, a weight detection assembly and a transfer assembly. The test tube support assembly comprises a bottom frame assembly and a clamp assembly. A jacking table is arranged on the top face of the bottom frame assembly in a protruding mode. The clamp assembly comprises a top frame plate and a supporting part, the top frame plate is connected to the supporting part, and a matching through hole is formed in the top frame plate. The clamp assembly is configured to be capable of being supported on the bottom frame assembly through the supporting part and matched with the through hole to correspond to the jacking table. And the matching through hole is configured to allow the test tube to pass through and be supported on the jacking table. The test tube is used for containing reagents. The weight detection assembly is provided with a bearing part and is used for detecting the weight of a test tube placed on the bearing part; the transfer assembly is used for transferring the test tubes between the test tube support assembly and the counter weight assembly. The device has the beneficial effects of being high in detection efficiency, low in manpower dependence and accurate in test tube cutting and clamping.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of gene detection, in particular, to a fluid detection system and a gene sequencing kit. BACKGROUND

[0002] Before the gene sequencer is shipped, it needs to be tested whether the fluid liquid system is qualified. In some known technologies, the method is to draw and discharge fluid through the gene sequencer, and then compare the weight of the fluid before and after drawing and discharging to calculate whether the fluid liquid system is qualified. In the process, more manual work is needed, such as manual taking and placing of reagent boxes, manual weighing, manual recording and calculating data, etc. There are problems of low efficiency, low accuracy, easy to make mistakes, high dependence on manpower, etc. CONTENT OF THE UTILITY MODEL

[0003] The present application provides a fluid detection system and a gene sequencing kit to solve the problem of low detection efficiency of the fluid detection in the known technology.

[0004] In a first aspect, the present application provides a fluid detection system, which comprises a test tube support assembly, a weight detection assembly and a transfer assembly. The test tube support assembly comprises a chassis assembly and a clamp assembly. The top surface of the chassis assembly is provided with a jacking table. The clamp assembly comprises a top rack plate and a support part, and the top rack plate is connected to the support part. The top rack plate is provided with a matching through hole. The clamp assembly is configured to be supported on the chassis assembly by the support part, and the matching through hole corresponds to the jacking table. The matching through hole is configured to allow the test tube to pass through and be supported on the jacking table. The test tube is used to contain reagents. The weight detection assembly has a bearing part, and the weight detection assembly is used to detect the weight of the test tube placed on the bearing part. The transfer assembly is used to transfer the test tube between the test tube support assembly and the weight detection assembly.

[0005] The use of the fluid detection system in the present application is that the test tube after being drawn or discharged by the gene sequencer is placed on the test tube support assembly. The transfer assembly transfers the test tube from the test tube support assembly to the weight detection assembly for weighing. After the weighing is completed, the transfer assembly transfers the test tube back to the test tube support assembly or other positions. The operation is convenient and fast, easy to realize automatic detection, and the detection efficiency is high. In addition, the test tube support assembly is provided with a jacking table through the chassis assembly. When the clamp assembly carries the test tube and supports it on the chassis assembly, the test tube can be lifted, which is beneficial to the accurate grabbing of the test tube by the transfer assembly.

[0006] In one possible implementation, the chassis assembly comprises a chassis plate. The chassis plate comprises a plate body, a jacking table and two support tables. The two support tables are respectively connected to the top surface of the plate body and are spaced apart from each other along a first direction. The jacking table is located between the two support tables. The top rack plate is respectively matched to the two support tables along the two ends of the first direction.

[0007] In a possible implementation, the support part includes two support blocks. The two support blocks are respectively connected to two ends of the top frame plate along the first direction. The support table is provided with a matching groove, and the two support blocks are respectively matched in the matching grooves of the two support tables and supported on groove bottom surfaces of the matching grooves.

[0008] In a possible implementation, the support block has a support surface and a threaded column protruding from the support surface. The top frame plate is provided with two through holes, the top frame plate is supported on the support surface, and the two through holes are respectively sleeved on the threaded columns of the two support blocks. The clamp assembly further includes two connecting nuts, the two connecting nuts are respectively threadedly connected to the two threaded columns, and the top frame plate is locked on the support blocks.

[0009] In a possible implementation, the support part includes four support feet, and the four support feet are respectively connected to four corners of the top frame plate. The support table is provided with a matching groove, and two support feet are matched in one of the matching grooves, and the other two support feet are matched in the other matching groove.

[0010] In a possible implementation, the base frame assembly further includes a base plate and a plurality of support columns. The base frame plate is supported and connected to the base plate through the plurality of support columns.

[0011] In a possible implementation, the test tube includes a tube body and a tube rim. The tube rim is protruded on an outer circumferential surface of an open end of the tube body. When the clamp assembly is supported on the base frame assembly, the lifting table lifts the test tube, so that the tube rim is separated from the clamp assembly.

[0012] In a possible implementation, the fluid detection system further includes a workbench. The test tube support assembly, the weight detection assembly, and the transfer assembly are respectively installed on the workbench.

[0013] In a possible implementation, the fluid detection system further includes a display device. The display device is electrically connected to the weight detection assembly, and is used to display the weight detected by the weight detection assembly.

[0014] In a second aspect, a gene sequencing kit is provided, which includes a gene sequencer and the fluid detection system as described above. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some of the embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.

[0016] Figure 1 It is a perspective view of the fluid detection system of an embodiment of the present application.

[0017] Figure 2Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application. Figure 1 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application.

[0018] Figure 3 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application. Figure 2 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application.

[0019] Figure 4 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application. Figure 2 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application.

[0020] Figure 5 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application. Figure 1 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application.

[0021] Figure 6 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application. Figure 5 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application.

[0022] Figure 7 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application. Figure 5 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application.

[0023] Figure 8 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application. Figure 1 Fig. 1 is a perspective view of a tube rack assembly according to an embodiment of the present application.

[0024] Main element symbol explanation: 300-tube; 310-tube body; 320-tube edge; 100-fluid detection system; 110, 110a-tube rack assembly; 10-chassis assembly; 11-chassis plate; 11a-plate body; 11b-top lifting platform; 11c-support platform; 12-bottom plate; 13-support column; 20, 20a-clamp assembly; 21-top chassis plate; 22-support part; 23-support block; 23a-threaded column; 24-connection nut; 25-support foot; 26-washer; 27-matching plate; 30-checking weight assembly; 31-bearing part; 40-transport assembly; 41-transport manipulator; 42-clamping jaw; 50-workbench; 60-display device; C1-matching groove; C2-placing groove; P1-supporting surface; K1-matching through hole; K2-through hole; K3-lack hole; Y1-first direction. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all embodiments of the present application.

[0026] It is to be understood that where an element such as a layer, region or substrate is described as being "on" another element, it can be directly on the other element or intervening elements can also be present. Where an element such as a layer, region or substrate is described as being "connected" to another element, it can be directly connected to the other element or intervening elements can also be present. Where an element such as a layer, region or substrate is described as being "positioned on" another element, it can be directly positioned on the other element or intervening elements can also be present. Relative terms are used to describe one element's relationship to another element as illustrated in the drawings. Terms such as "vertical", "horizontal", "left", "right", and the like are used herein for the purpose of illustration only.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used in the description of the application herein is for describing particular embodiments only and is not intended to be limiting of the application. The use herein of the terms "and / or" includes a set of one or more associated listed items.

[0028] Some embodiments of the application are described in detail. The following embodiments and features of the embodiments can be combined with each other in non-conflicting cases.

[0029] Embodiments

[0030] Reference Figure 1 The embodiments provide a fluid detection system 100 for testing whether a fluid system of a gene sequencer is qualified. The gene sequencer can determine a pathogenic gene by analyzing a full sequence of a gene, so as to achieve early prevention and treatment.

[0031] The fluid system is an important component of the gene sequencer, which provides sufficient and uniform biochemical reagents for a sequencing chip. The sequencing process needs to detect nucleic acid bases one by one, and the identification of a single base needs to go through three steps of synthesis, identification and removal. Different biochemical reagents need to be input to the sequencing chip for reaction in the synthesis and removal process.

[0032] Some related technologies increase the amount of reagents on the sequencing chip to improve the accuracy of base identification, but this increases the amount of reagents and the cost of sequencing. After the sequencing is completed, the cleaning of the fluid pipeline is also time-consuming and laborious.

[0033] The fluid system of some gene sequencers includes functions of reagent selection, reagent transfer, accurate pumping, waste liquid collection, liquid path cleaning and the like.

[0034] The fluid detection system 100 provided by the embodiments can be used to test whether the functions of the fluid system of the gene sequencer, such as pumping accuracy, pumping precision, system airtightness and the like, are qualified.

[0035] In some related technologies, a fluid detection device is used to draw and discharge reagents by a gene sequencer, and then the reagents before and after the drawing and discharging are weighed and compared to calculate whether the drawing and discharging function of the fluid liquid system is qualified. In the process, more manual work is required, such as manual taking and placing of reagent boxes, manual weighing, manual recording and calculating data, etc., which has problems of low efficiency, low accuracy, easy to make mistakes, high dependence on manpower, etc.

[0036] Therefore, the embodiment provides a fluid detection system 100 which can efficiently and accurately realize fluid system detection and reduce the dependence on manpower. The following will be exemplarily introduced.

[0037] Referring to Figure 1 The fluid detection system 100 provided by the embodiment includes a workbench 50, a test tube support assembly 110, a weight detection assembly 30, a transfer assembly 40 and a display device 60. The test tube support assembly 110, the weight detection assembly 30, the transfer assembly 40 and the display device 60 are respectively installed on the tabletop of the workbench 50.

[0038] The test tube support assembly 110 can carry a test tube 300 for containing reagents used in gene sequencing. The reagents can be cleaning reagents, sequencing reagents, etc., which are not limited here.

[0039] The weight detection assembly 30 is used to detect the weight of the test tube 300. The weight detection assembly 30 can be an electronic scale with a ten-thousandth grade precision, so as to ensure the weight detection precision. The weight detection assembly 30 can have a data transmission function to facilitate real-time communication with the upper computer software.

[0040] The display device 60 is electrically connected to the weight detection assembly 30 and is used to display the weight detected by the weight detection assembly 30. The display device 60 can be a desktop display, an industrial display screen, etc. After the test tube 300 is placed on the weight detection assembly 30, a weight detection instruction can be automatically triggered by software to instruct the weight detection assembly 30 to weigh. After the weighing is completed, the data detected by the weight detection assembly 30 can be transmitted to an upper computer (such as a computer), and after being processed by the upper computer software, the detection data is displayed on a suitable interface of the display device 60. After the weight detection is completed, the upper computer can instruct the transfer assembly 40 to take the test tube 300 back to the original place or transfer it to a set position.

[0041] The fluid detection system 100 in the embodiment can perform weight detection according to a predetermined sequence or a flexibly set sequence.

[0042] The fluid detection system 100 can also be provided with an alarm mechanism, which can send a reminder through sound, light, etc. when the detection result does not meet the set requirements.

[0043] The weight detection assembly 30 has a bearing portion 31, and is configured to detect the weight of the test tube 300 placed on the bearing portion 31.

[0044] The transfer assembly 40 is configured to transfer the test tube 300 between the test tube holder assembly 110 and the weight detection assembly 30. In the present embodiment, the transfer assembly 40 includes a transfer robot 41 and a gripper 42. The gripper 42 can be specially configured to fit the test tube 300, and can stably and accurately pick up or put down the test tube 300. The transfer robot 41 can be translated and rotated. The gripper 42 is connected to the end of the transfer robot 41 and can be moved under the driving of the transfer robot 41 to transfer the test tube 300.

[0045] Figures 2-4 A test tube holder assembly 110 of the present embodiment is shown.

[0046] Referring to Figures 2-4 In the present embodiment, the test tube holder assembly 110 includes a base assembly 10 and a clamp assembly 20. The clamp assembly 20 can position and bear one or more test tubes 300. In use, the clamp assembly 20 can be placed on the base assembly 10 with the test tubes 300, or can be taken away from the base assembly 10.

[0047] The top surface of the base assembly 10 is provided with a jacking table 11b. The clamp assembly 20 includes a top rack plate 21 and a support portion 22, the top rack plate 21 is connected to the support portion 22, and the top rack plate 21 is provided with a matching through hole K1. The clamp assembly 20 is configured to be supported on the base assembly 10 by the support portion 22, and the matching through hole K1 corresponds to the jacking table 11b. The matching through hole K1 is configured to allow the test tube 300 to pass through and be supported on the jacking table 11b. In some embodiments, the support portion 22 can be a reagent bottle for containing reagents or waste liquid.

[0048] The use of the fluid detection system 100 in the present embodiment is that the test tube 300 after being pumped or drained by the gene sequencer is placed on the test tube holder assembly 110, the transfer assembly 40 transfers the test tube 300 from the test tube holder assembly 110 to the weight detection assembly 30 for weighing, and after the weighing is completed, the transfer assembly 40 transfers the test tube 300 back to the test tube holder assembly 110 or other positions, which is convenient and fast, and easy to realize automatic detection. In addition, the test tube holder assembly 110 is provided with the jacking table 11b by the base assembly 10, which can lift the test tube 300 when the clamp assembly 20 carries the test tube 300 to be supported on the base assembly 10, so that the test tube 300 extends out of the top rack plate 21, which is beneficial to the accurate grabbing of the test tube 300 by the transfer assembly 40, and beneficial to the separation of the test tube 300 from the top rack plate 21.

[0049] Optionally, the test tube 300 includes a tube body 310 and a tube rim 320 protruding from the outer circumferential surface of the open end of the tube body 310. Before the clamp assembly 20 is supported on the base assembly 10, the protruding rim of the test tube 300 is pressed against the top surface of the top base plate 21 under the action of gravity. When the clamp assembly 20 is supported on the base assembly 10, the lifting platform 11b lifts the test tube 300, so that the tube rim 320 is separated from the clamp assembly 20.

[0050] In some embodiments, the top base plate 21 is provided with a gasket 26 at a position corresponding to the fitting through hole K1 in the top base plate 21. The gasket 26 is arranged between the tube rim 320 of the test tube 300 and the top base plate 21. In this case, when the clamp assembly 20 is supported on the base assembly 10, the tube rim 320 is separated from the gasket 26.

[0051] Optionally, in the present embodiment, the base assembly 10 further includes a base plate 12 and a plurality of support columns 13. The base plate 11 is supported and connected to the base plate 12 by the plurality of support columns 13. The base plate 12 can be installed on the workbench 50 by locking members (e.g., screws).

[0052] Optionally, in the present embodiment, the top base plate 21 is provided with a plurality of fitting through holes K1, and each fitting through hole K1 is used to fit one test tube 300. The coverage area of the lifting platform 11b includes part or all of the test tubes 300. In this way, the clamp assembly 20 can carry a plurality of test tubes 300 at a time, and the fluid detection system 100 can sequentially perform the operations of grabbing and transferring, weighing, and returning the test tubes 300 to the clamp assembly 20, so as to complete the weighing of the plurality of test tubes 300.

[0053] In the present embodiment, the base assembly 10 includes a base plate 11. The base plate 11 includes a plate body 11a, a lifting platform 11b, and two support platforms 11c. The two support platforms 11c are respectively connected to the top surface of the plate body 11a and are spaced apart from each other along the first direction Y1. The lifting platform 11b is located between the two support platforms 11c. The top base plate 21 is fitted to the two support platforms 11c at the two ends thereof along the first direction Y1. In this way, the top base plate 21 is spaced apart from the base plate 11, thereby providing a protruding space for the lifting platform 11b.

[0054] Optionally, the support part 22 comprises two support blocks 23. The two support blocks 23 are respectively connected to the two ends of the top frame plate 21 along the first direction Y1. The support table 11c is provided with a matching groove C1, and the two support blocks 23 are respectively matched in the matching grooves C1 of the two support tables 11c and supported on the groove bottom surfaces of the matching grooves C1. In this way, when the clamp assembly 20 supports the test tube 300 on the support table 11c, the clamp assembly 20 will be lowered to the groove bottom surface of the matching groove C1, and during the lowering process, the bottom of the test tube 300 is limited by the lifting table 11b while the clamp assembly 20 continues to be lowered, so that the test tube 300 extends relative to the top frame plate 21 of the clamp assembly 20, which is beneficial for subsequent grabbing.

[0055] In the embodiment, the support block 23 has a support surface P1 and a threaded column 23a protruding from the support surface P1. The top frame plate 21 is provided with two through holes K2, the top frame plate 21 is supported on the support surface P1, and the two through holes K2 are respectively sleeved on the threaded columns 23a of the two support blocks 23. The clamp assembly 20 further comprises two connecting nuts 24, which are respectively threadedly connected to the two threaded columns 23a and lock the top frame plate 21 to the support block 23. In this way, the top frame plate 21 and the support block 23 of the clamp assembly 20 are detachable, and when needed, support blocks 23 of different heights can be replaced to adapt to test tubes 300 of different heights, which is convenient to use.

[0056] Again referring to Figure 1 , in the fluid detection system 100 of the embodiment, in addition to being provided with Figures 2-4 the test tube support assembly 110, the workbench 50 is also provided with another test tube support assembly 110a. The test tube support assembly 110a will be described below. Figures 5-7

[0057] Referring to Figures 5-7 , the test tube support assembly 110a comprises a base frame assembly 10 and another clamp assembly 20a. The base frame assembly 10 can be the same as Figures 2-4 . The clamp assembly 20a adopts a different design.

[0058] The clamp assembly 20a comprises a top frame plate 21 and a support part 22, and the support part 22 comprises four support feet 25 respectively connected to the four corners of the top frame plate 21. Optionally, the four support feet 25 are integrally formed on the top frame plate 21. In the embodiment, the support feet 25 integrally formed on the top frame plate 21 can reliably support the top frame plate 21, and the integrally formed structure reduces the number of components.

[0059] In the embodiment, two of the four support feet 25 are matched in one matching groove C1 of the base frame assembly 10, and the other two support feet 25 are matched in another matching groove C1.

[0060] ​The clamp assembly 20a of this embodiment, when supported on the chassis assembly 10, has the supporting feet 25 supported on the matching grooves C1, and can also make the lifting platform 11b lift the test tube 300, so as to facilitate the accurate clamping of the test tube 300. For details, please refer to the description of the embodiment of the lifting platform 11b, which will not be repeated here. Figures 2-4

[0061] Optionally, the clamp assembly 20a of this embodiment has the top frame plate 21 provided with the holes K3, and the matching plates 27 are matched in the holes K3. Part of the matching through holes K1 of the top frame plate 21 are provided in the matching plates 27. For example, as shown in the figure, the holes K3 and the matching plates 27 are four respectively, and the four matching plates 27 are matched in the four holes K3 respectively. Each of the matching plates 27 is provided with one matching through hole K1. Through the detachable matching plates 27, the matching plates 27 with different sizes of matching through holes K1 can be replaced to carry test tubes 300 with different sizes, so as to provide the versatility of the clamp assembly 20a.

[0062] For details, please refer to the description of the lifting platform 11b, which will not be repeated here. Figure 8 Optionally, the carrying part 31 of the detection assembly 30 of this embodiment is provided with the placing grooves C2, and the shape of the placing grooves C2 matches the bottom of the test tube 300, so as to facilitate the stable placement of the test tube 300 on the carrying part 31.

[0063] This embodiment also provides a gene sequencing kit, which comprises a gene sequencer and the fluid detection system 100 described above. Through the fluid detection system 100 described above, it can be efficiently and accurately detected whether the fluid system in the gene sequencer is qualified.

[0064] The above embodiments are only used to illustrate the technical solutions of the present application and not to limit it. Although the present application has been described in detail with reference to the above preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the spirit and scope of the present application.​

Claims

1. A fluid detection system, characterized by, The fluid detection system comprises: a test tube holder assembly comprising a base frame assembly and a clamp assembly; a top surface of the base frame assembly is provided with a lifting platform; the clamp assembly comprises a top frame plate and a support part, the top frame plate is connected to the support part, and the top frame plate is provided with a matching through hole; the clamp assembly is configured to be supported on the base frame assembly by the support part, and the matching through hole corresponds to the lifting platform; the matching through hole is configured to allow a test tube to pass through and be supported on the lifting platform; the test tube is used to contain reagents; a weight detection assembly having a bearing part, the weight detection assembly being used to detect the weight of the test tube placed on the bearing part; and a transfer assembly used to transfer the test tube between the test tube holder assembly and the weight detection assembly.

2. The fluid detection system according to claim 1, wherein: the base frame assembly comprises a base frame plate; the base frame plate comprises a plate body, the lifting platform and two support platforms; the two support platforms are respectively connected to the top surface of the plate body and are spaced apart from each other along a first direction; the lifting platform is located between the two support platforms; the two ends of the top frame plate along the first direction are respectively matched with the two support platforms.

3. The fluid detection system according to claim 2, wherein: the support part comprises two support blocks; the two support blocks are respectively connected to the two ends of the top frame plate along the first direction; the support platforms are provided with matching grooves, and the two support blocks are respectively matched in the matching grooves of the two support platforms and supported on the groove bottom surfaces of the matching grooves.

4. The fluid detection system according to claim 3, wherein: the support blocks have support surfaces and threaded columns protruding from the support surfaces; the top frame plate is provided with two through holes, the top frame plate is supported on the support surfaces, and the two through holes are respectively sleeved on the threaded columns of the two support blocks; the clamp assembly further comprises two connecting nuts, the two connecting nuts are respectively threadedly connected to the two threaded columns and lock the top frame plate on the support blocks.

5. The fluid detection system according to claim 2, wherein: the support part comprises four support feet, and the four support feet are respectively connected to the four corners of the top frame plate; the support platforms are provided with matching grooves, and two of the support feet are matched in one of the matching grooves, and the other two support feet are matched in the other matching groove.

6. The fluid detection system according to claim 2, wherein: the base frame assembly further comprises a base plate and a plurality of support columns; the base frame plate is supported and connected to the base plate by the plurality of support columns.

7. The fluid detection system according to claim 1, wherein: the test tube comprises a tube body and a tube rim, and the tube rim is provided on the outer circumferential surface of the open end of the tube body; when the clamp assembly is supported on the base frame assembly, the lifting platform lifts the test tube, so that the tube rim is separated from the clamp assembly.

8. The fluid detection system according to claim 1, further comprising a workbench. ​ The test tube rack assembly, the weight detection assembly and the transfer assembly are respectively installed on the workbench.

9. The fluid detection system of claim 1, wherein: The fluid detection system further comprises a display device; The display device is electrically connected to the weight detection assembly, and is configured to display the weight detected by the weight detection assembly.

10. A kit for genetic sequencing, characterized in that, Comprising: a gene sequencer, and The fluid detection system of any one of claims 1-9.

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