Protein filler preassembled column loader

Abstract

The utility model discloses a protein purification filler preloaded column loader which comprises an outer sleeve and an embedded pipe, the outer sleeve and the embedded pipe are concentric hollow pipe fittings, the embedded pipe is embedded in the outer sleeve, an interlayer between the outer sleeve and the embedded pipe forms a purification bin, the ends of the same side of the outer sleeve and the embedded pipe are fixed to a rear end socket of a cylindrical structure, and an air inlet channel communicated with the interior of the embedded pipe is formed in the rear end socket. A feeding channel communicated with the interior of the purification bin is arranged in the rear sealing head, a feeding pipe with a tubular structure is arranged on the side wall of the rear sealing head, and the exterior of the feeding channel is communicated with the feeding pipe; a switch valve capable of controlling the opening and closing of the air inlet channel is arranged on the rear sealing head; a rubber seal head made of rubber is sealed at the end part of one side, far away from the rear seal head, of the embedded pipe, and the rubber seal head and the rear seal head are connected through a tension spring; the device has the beneficial effects that the liquid protein purification filler can be subjected to conventional purification operation, and the purification efficiency is high.

Description

Technical Field

[0001] This utility model belongs to the technical field of protein purification equipment, and specifically relates to a protein packing pre-loaded column carrier. Background Technology

[0002] The patent document with publication number CN203091244U mentions that in the life sciences field, packing materials are frequently used for the separation and purification of proteins. The use of packing materials is categorized into chromatography and batch adsorption methods, depending on whether a chromatography column is used; the former is more widely used. When using chromatography, the packing material needs to be uniformly packed into a pre-packed chromatography column. When the mixed protein solution passes through this column at a certain flow rate, it selectively adsorbs onto the packing material.

[0003] However, existing protein packing column carriers are generally designed for solid packing materials, such as a simple protein purification device with publication number CN207193167U. With scientific advancements, liquid protein purification packing materials have gradually appeared in some purification experiments with high purification requirements, but existing technologies rarely have carrier mechanisms for liquid protein packing column carriers. Utility Model Content

[0004] The purpose of this invention is to provide a protein filler pre-loaded column carrier to solve the technical problems mentioned in the background section.

[0005] The above-mentioned technical objective of this utility model is achieved through the following technical solution:

[0006] A pre-packed column carrier for protein purification filler includes: an outer tube and an inner tube; the outer tube and the inner tube are concentric hollow tubes, with the inner tube embedded inside the outer tube, and the interlayer between the outer tube and the inner tube forming a purification chamber; the ends of the outer tube and the inner tube on the same side are fixed to a cylindrical rear end cap; the rear end cap has an air inlet channel communicating with the interior of the inner tube, and a feed channel communicating with the interior of the purification chamber; the side wall of the rear end cap has a tubular feed pipe, and the feed channel communicates with the outside of the feed pipe; the rear end cap has a switch valve that can control the opening and closing of the air inlet channel; the end of the inner tube away from the rear end cap is sealed with a rubber end cap, and the rubber end cap and the rear end cap are connected by a tension spring, which has a shortening elastic force tendency, so that the rubber end cap squeezes and seals the end of the inner tube.

[0007] Furthermore, to facilitate the titration and discharge of protein solution in the purification chamber, a conical tubular structure is embedded at the end of the outer tube away from the rear end cap to form an injection tube.

[0008] Furthermore: In order to facilitate the injection of protein solution into the purification chamber and to prevent the protein solution from leaking out of the purification chamber, a first one-way pipe is fixed to the end of the feed pipe, and a second one-way pipe is fixed to the end of the rear end cap away from the outer tube. The air inlet channel is connected to the outside and the second one-way pipe. The internal structure and function of the first one-way pipe and the second one-way pipe are the same, both allowing fluid to enter the outer tube or the inner tube in one direction only from the outside.

[0009] Furthermore, to facilitate the connection of the second one-way tube to a conventional syringe, the end of the second one-way tube away from the rear end cap is extended outward and fitted with a rubber ring.

[0010] Furthermore: In order to facilitate the connection of the second one-way tube to some precision pneumatic or hydraulic tools, the outer ring of the second one-way tube is provided with external threads for connecting external equipment.

[0011] Furthermore: In order to prevent the protein purification packing material from directly entering the injection tube during protein purification, the rubber end cap mainly consists of a disc-shaped sealing plate and a column-shaped sealing column. The diameter of the sealing column is smaller than the inner diameter of the inner tube and is movably embedded in the inner tube. The near ends of the sealing column and the tension spring are fixedly connected. Under the tension of the tension spring, the sealing plate closes the port of the inner tube. A convex annular structure is provided on the outer periphery of the sealing plate near the sealing column to form a guide ring. The inner diameter of the guide ring is larger than the outer diameter of the inner tube. The sealing plate, sealing column, and guide ring are integrally formed rubber structures.

[0012] Furthermore: the first one-way tube includes: a tube wall, a flexible plug, a hollow plug, a material passage, a sliding cavity, a sliding column, and a spring; the tube wall is a cylindrical structure, with a cylindrical hollow plug fixedly embedded in the tube wall, a material passage connecting both sides of the hollow plug on the hollow plug, a cylindrical sliding cavity at the center of the hollow plug, a cylindrical sliding column slidably embedded in the sliding cavity, a flexible plug made of rubber fixed at the outer end of the sliding column, and an inwardly convex annular structure forming a tube opening at the end of the tube wall away from the rear end cap; a spring is concentrically embedded on the sliding column between the flexible plug and the hollow plug, the spring having an elongating elastic force tendency, and under the elastic force of the spring, the flexible plug presses and seals the tube opening.

[0013] Furthermore, to facilitate observation of the protein purification filler and protein solution inside the outer and inner tubes, both the outer and inner tubes are made of transparent material.

[0014] In summary, this utility model has the following beneficial effects:

[0015] ① This allows for routine purification of liquid protein purification packing materials: With the setup of an outer tube, inner tube, and purification chamber, during protein purification, a suitable syringe is used; a tube of sterile nitrogen is drawn, the syringe tip is embedded inside the rubber ring, the cap on the end of the injection tube is removed, and the entire assembly is placed vertically with the injection tube facing downwards. The valve is then opened, and the syringe is slowly pushed, injecting sterile nitrogen into the second one-way tube. The second one-way tube guides the sterile nitrogen from the inlet channel into the inner tube, increasing the pressure within the inner tube. The protein purification packing material at the bottom of the inner tube pushes open the rubber cap at the end of the inner tube, allowing the packing material to enter the purification chamber. An equal volume of purified protein solution is discharged from the injection tube, completing the purification process.

[0016] ② High purification efficiency: Due to the presence of the guide ring, and because the entire device is vertically positioned with the injection tube facing downwards during use, and the purification is titration-based, the flow rate of the protein purification packing material entering the purification chamber is relatively slow. Therefore, the low-density protein purification packing material entering the purification chamber from the inner tube will quickly rise due to density issues under the guidance of the guide ring and the obstruction of the sealing plate. As a volume exchange, it will squeeze out the protein liquid near the injection tube. The rising protein purification packing material will also refine itself as it floats in the purification chamber, causing turbulence in the protein liquid within the chamber. This turbulence will disrupt the protein liquid in the purification chamber and ensure sufficient contact and reaction between the protein liquid and the purification packing material, thereby improving the purification rate of the protein liquid. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0018] Figure 2 This is a partial cross-sectional structural diagram of the present invention;

[0019] Figure 3 This is a schematic diagram of the air intake channel and the feed channel in this utility model;

[0020] Figure 4 This is an exploded schematic diagram illustrating the conduction principle of the outer tube and the inner tube in this utility model;

[0021] Figure 5 yes Figure 4 Enlarged view of the structure at point A in the middle;

[0022] Figure 6 This is a partial cross-sectional view of the first unidirectional tube in this utility model.

[0023] In the diagram, 1. Outer tube; 2. Inner tube; 3. Purification chamber; 4. Injection tube; 5. Rear end cap; 6. First one-way tube; 7. Feed tube; 8. Second one-way tube; 9. Rubber ring; 10. Switch valve; 11. Rubber end cap; 12. Tension spring; 51. Air inlet channel; 52. Feed channel; 61. Tube wall; 62. Flexible plug; 63. Hollow plug; 64. Material passage; 65. Sliding cavity; 66. Sliding column; 67. Spring; 68. Tube opening; 111. Sealing column; 112. Sealing plate; 113. Guide ring. Detailed Implementation

[0024] The present invention will be further described in detail below with reference to the accompanying drawings.

[0025] Example:

[0026] Please see Figures 1-6 The present invention provides the following technical solution:

[0027] A protein purification packing column carrier includes: an outer tube 1 and an inner tube 2; the outer tube 1 and the inner tube 2 are concentric hollow tubes, the inner tube 2 is embedded in the outer tube 1, the interlayer between the outer tube 1 and the inner tube 2 forms a purification chamber 3, the same-side ends of the outer tube 1 and the inner tube 2 are fixed on a cylindrical rear end cap 5, the rear end cap 5 has an air inlet channel 51 that communicates with the interior of the inner tube 2, the rear end cap 5 has a feed channel 52 that communicates with the interior of the purification chamber 3, and the side wall of the rear end cap 5 has a tubular feed pipe 7, the feed channel 52 communicates with the outside of the feed pipe 7; The rear end cap 5 is provided with a switch valve 10 that can control the opening and closing of the air intake channel 51 (the switch valve 10 is a direct application of this application and is not within the scope of protection of this application, so its specific structure is omitted. It can be a ball valve, in which a perforated ball core is embedded in the air intake channel 51, and the opening and closing of the air intake channel 51 is controlled by rotating the ball core); the end of the inner tube 2 away from the rear end cap 5 is sealed with a rubber end cap 11 made of rubber. The rubber end cap 11 and the rear end cap 5 are connected by a tension spring 12. The tension spring 12 has a shortening elastic tendency, so that the rubber end cap 11 squeezes and seals the end of the inner tube 2.

[0028] The outer sleeve 1, at the end furthest from the rear end cap 5, has a tapered tubular structure embedded to form an injection tube 4.

[0029] The end of the feed pipe 7 is fixed with a first one-way pipe 6, and the end of the rear end cap 5 away from the outer sleeve 1 is fixed with a second one-way pipe 8. The air inlet channel 51 is connected to the outside and the second one-way pipe 8. The internal structure and function of the first one-way pipe 6 and the second one-way pipe 8 are the same, both allowing fluid to enter the outer sleeve 1 or the inner sleeve 2 in one direction only from the outside.

[0030] The end of the second one-way tube 8 away from the rear end cap 5 extends outward and is fitted with a rubber ring 9 (the outer diameter of the syringe head used must be larger than the inner diameter of the rubber ring 9).

[0031] The outer ring of the second one-way tube 8 is provided with external threads for connecting to external equipment.

[0032] The rubber end cap 11 mainly consists of a disc-shaped sealing plate 111 and a column-shaped sealing post 112. The diameter of the sealing post 112 is smaller than the inner diameter of the inner tube 2 and is movably embedded in the inner tube 2. The sealing post 112 and the near ends of the tension spring 12 are fixedly connected. Under the tension of the tension spring 12, the sealing plate 111 closes the port of the inner tube 2. The outer periphery of the sealing plate 111 near the sealing post 112 is provided with an outwardly protruding annular structure forming a guide ring 113. The inner diameter of the guide ring 113 is larger than the outer diameter of the inner tube 2. The sealing plate 111, the sealing post 112, and the guide ring 113 are integrally formed rubber structures.

[0033] The first one-way tube 6 includes: a tube wall 61, a flexible plug 62, a hollow plug 63, a material passage 64, a sliding cavity 65, a sliding column 66, and a spring 67. The tube wall 61 is a cylindrical structure, and a cylindrical hollow plug 63 is embedded and fixed inside the tube wall 61. The hollow plug 63 is provided with a material passage 64 that connects both sides of the hollow plug 63. A cylindrical sliding cavity 65 is provided at the center of the hollow plug 63. A cylindrical sliding column 66 is slidably embedded in the sliding cavity 65. A rubber flexible plug 62 is fixed at the outer end of the sliding column 66. The end of the tube wall 61 away from the rear end cap 5 is provided with an inwardly convex annular structure to form a tube opening 68. A spring 67 is concentrically embedded on the sliding column 66 between the flexible plug 62 and the hollow plug 63. The spring 67 has an elongating elastic tendency. Under the elastic force of the spring 67, the flexible plug 62 compresses and seals the tube opening 68.

[0034] Both the outer tube 1 and the inner tube 2 are made of transparent materials (such as glass or acrylic).

[0035] Brief description of usage:

[0036] When using (this pre-packed column is only suitable for low-density protein purification packing for high-density protein solutions), first open the switch valve 10 to inject the protein purification packing into the inner tube 2.

[0037] Before conducting a protein purification experiment, the protein solution to be purified is injected into the feed pipe 7 through the first one-way tube 6. Under pressure, the protein solution to be purified passes through the first one-way tube 6, pushes open the flexible plug 62, and the spring 67 is compressed. The protein solution to be purified enters the first one-way tube 6 through the gap between the flexible plug 62 and the pipe opening 68, and then enters the feed pipe 7 through the feed channel 64. The protein solution to be purified in the feed pipe 7 enters the purification chamber 3 through the feed channel 52 for storage, awaiting purification. After the protein purification packing is filled, the flexible plug 62 loses external pressure, and under the action of the spring 67, the flexible plug 62 compresses and seals the pipe opening 68 again; then the switch valve 10 is closed.

[0038] During protein purification, a suitable syringe is used (the outer diameter of the syringe tip must be larger than the inner diameter of the rubber ring 9); a tube of sterile nitrogen is drawn, the syringe tip is embedded inside the rubber ring 9, the cap on the end of the injection tube 4 is removed, and the entire utility model is placed vertically with the injection tube 4 facing downwards. Then, the switch valve 10 is opened, and the syringe is slowly pushed. The syringe slowly injects sterile nitrogen into the second one-way tube 8. The second one-way tube 8 introduces sterile nitrogen from the air inlet channel 51 into the inner tube 2, causing the pressure inside the inner tube 2 to increase. The protein purification packing material at the bottom of the inner tube 2 pushes open the rubber cap 11 that is pressed at the end of the inner tube 2, and the protein purification packing material in the inner tube 2 enters the purification chamber 3. An equal volume of purified protein solution is discharged from the injection tube 4, completing the purification operation (during operation, the first two drops of purified protein solution are generally not completely purified and are often discarded).

[0039] With the establishment of the guide ring 113, since the entire utility model is placed vertically with the injection tube 4 facing downwards during use, and the purification is titration purification, the flow rate of the protein purification packing entering the purification chamber 3 is relatively slow. Therefore, the low-density protein purification packing entering the purification chamber 3 from the embedded tube 2 will quickly float to the surface due to density issues under the guidance of the guide ring 113 and the blocking effect of the sealing plate 111. As a volume exchange, it will squeeze out the protein liquid near the injection tube 4. The floating protein purification packing will become finer as it floats in the purification chamber 3, and will also cause the protein liquid in the purification chamber 3 to form turbulence. The turbulence will disturb the protein liquid in the purification chamber 3 and make full contact between the protein liquid and the purification packing to carry out an adsorption reaction (only adsorbing impurities and not dissolving with proteins), thereby improving the purification rate of the protein liquid and reducing errors in biological experiments or biological product manufacturing.

[0040] Meanwhile, because the syringe is pre-filled with sterile nitrogen, the gas entering the syringe will minimize contamination of the protein solution and maintain its purity.

[0041] This specific embodiment is merely an explanation of the present utility model and is not intended to limit the present utility model. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but as long as they are within the scope of the claims of the present utility model, they are protected by patent law.

Claims

1. A protein filler pre-packed column carrier, comprising: Outer tube (1), inner tube (2); characterized in that: the outer tube (1) and the inner tube (2) are concentric hollow tubes, the inner tube (2) is embedded in the outer tube (1), the interlayer between the outer tube (1) and the inner tube (2) forms a purification chamber (3), the same-side ends of the outer tube (1) and the inner tube (2) are fixed on a cylindrical rear end cap (5), the rear end cap (5) is provided with an air inlet channel (51) communicating with the inside of the inner tube (2), and the rear end cap (5) is provided with a feed channel (52) communicating with the inside of the purification chamber (3). The rear end cap (5) has a tubular feed pipe (7) on its side wall, and the feed channel (52) is connected to the outside of the feed pipe (7). The rear end cap (5) has a switch valve (10) that can control the opening and closing of the air intake channel (51). The end of the inner tube (2) away from the rear end cap (5) is sealed with a rubber end cap (11). The rubber end cap (11) and the rear end cap (5) are connected by a tension spring (12). The tension spring (12) has a shortening elastic tendency, so that the rubber end cap (11) squeezes and seals the end of the inner tube (2).

2. The protein filler pre-packed column carrier according to claim 1, characterized in that: The outer sleeve (1) has a tapered tubular structure embedded at the end away from the rear end cap (5) to form an injection tube (4).

3. The protein filler pre-packed column carrier according to claim 1, characterized in that: The feed pipe (7) is fixed with a first one-way pipe (6) at one end, and the end of the rear end cap (5) away from the outer sleeve (1) is fixed with a second one-way pipe (8). The air inlet channel (51) is connected to the outside of the second one-way pipe (8). The internal structure and function of the first one-way pipe (6) and the second one-way pipe (8) are the same, allowing fluid to enter the outer sleeve (1) or the inner sleeve (2) in one direction only.

4. A protein filler pre-packed column carrier according to claim 3, characterized in that: The end of the second one-way tube (8) away from the rear end cap (5) extends outward and is fitted with a rubber ring (9).

5. A protein filler pre-packed column carrier according to claim 3, characterized in that: The outer ring of the second one-way tube (8) is provided with external threads for connecting external equipment.

6. A protein filler pre-packed column carrier according to claim 1, characterized in that: The rubber end cap (11) is mainly composed of a disc-shaped sealing plate (111) and a column-shaped sealing column (112). The diameter of the sealing column (112) is smaller than the inner diameter of the inner tube (2) and is movably embedded in the inner tube (2). The near ends of the sealing column (112) and the tension spring (12) are fixedly connected. The sealing plate (111) closes the port of the inner tube (2) under the tension of the tension spring (12). The outer periphery of the sealing plate (111) near the sealing column (112) is provided with an outwardly protruding annular structure to form a guide ring (113). The inner diameter of the guide ring (113) is larger than the outer diameter of the inner tube (2). The sealing plate (111), the sealing column (112), and the guide ring (113) are integrally formed rubber structures.

7. A protein filler pre-packed column carrier according to claim 3, characterized in that: The main body of the first one-way tube (6) is a tube wall (61) with a tube structure. A hollow plug (63) with a cylindrical structure is embedded in the tube wall (61). The hollow plug (63) is provided with a material passage (64) that connects the two sides of the hollow plug (63). A cylindrical sliding cavity (65) is provided in the center of the hollow plug (63). A cylindrical sliding column (66) is slidably embedded in the sliding cavity (65). A flexible plug (62) made of rubber is fixed at the outer end of the sliding column (66). A protruding annular structure is provided at the end of the tube wall (61) away from the rear end cap (5) to form a tube opening (68). A spring (67) is concentrically embedded on the sliding column (66) between the flexible plug (62) and the hollow plug (63). The spring (67) has an elongation tendency. Under the elastic force of the spring (67), the flexible plug (62) presses and seals the tube opening (68).

8. A protein filler pre-packed column carrier according to claim 1, characterized in that: Both the outer tube (1) and the inner tube (2) are made of transparent material.

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