Separation and purification device for removing endotoxin in plasmid DNA

Abstract

The utility model relates to the technical field of separation and purification devices, and discloses a separation and purification device for removing endotoxin in plasmid DNA (Deoxyribonucleic Acid), which comprises a fixed frame, a treatment barrel arranged in the middle of the fixed frame, a motor arranged at the side part of the treatment barrel, a mounting cover arranged at the end part of the treatment barrel, a handle arranged at the side part of the mounting cover, and a connecting pipe inserted in the middle of the mounting cover, a separation barrel is connected to the side portion of the connecting pipe, a protection assembly is arranged in the middle of the separation barrel, and the protection assembly arranged in the separation barrel comprises a separation groove, a contact rod, a sleeve block and a limiting assembly, so that plasmid DNA and endotoxin can be effectively separated, the purity of DNA is improved, and the separation efficiency is improved. Components such as a treatment barrel, a separation barrel and a collection barrel in the device are connected through connecting pipes and flow dividing pipes, an operator can control the flow direction of fluid conveniently, the operation process is simplified, through arrangement of structures such as a fixing frame, a mounting cover and the connecting pipes, the whole device has high stability in the operation process, and risks in the operation process are reduced.

Description

Technical Field

[0001] This utility model relates to the field of separation and purification equipment technology, specifically to a separation and purification equipment for removing endotoxins from plasmid DNA. Background Technology

[0002] Plasmid DNA is widely used in molecular biology research, genetic engineering, biotechnology, vaccine development, gene therapy, and other fields. As a gene vector, plasmids deliver foreign genes during cell transformation and are an indispensable tool in genetic engineering. Endotoxins are lipopolysaccharide components of the cell walls of Gram-negative bacteria, released when bacteria die or lyse. Endotoxins have negative effects on many biological experiments and medical applications, including inducing inflammatory responses, affecting cell function, and reducing the purity and stability of plasmid DNA.

[0003] Some existing methods involve cumbersome procedures that require professional personnel, which increases the risk to ordinary workers and raises experimental costs and time. Some efficient methods are also costly and unsuitable for large-scale application. Therefore, they do not meet the current needs. To address this, we propose a separation and purification device for removing endotoxins from plasmid DNA. Utility Model Content

[0004] This invention provides a separation and purification device for removing endotoxins from plasmid DNA, which has the beneficial effect of improving the ease of operation. It solves the problems mentioned in the background art that some methods in the prior art have cumbersome operation steps, require professional personnel to operate, which increases the danger of ordinary workers, increases experimental costs and time, and that some efficient methods are too expensive to be suitable for large-scale application.

[0005] This utility model provides the following technical solution: a separation and purification device for removing endotoxins from plasmid DNA, comprising a fixed frame, a processing tank installed in the middle of the fixed frame, a motor installed on the side of the processing tank, a mounting cover installed at the end of the processing tank, a handle installed on the side of the mounting cover, a connecting tube inserted into the middle of the mounting cover, a separation tank connected to the side of the connecting tube, and a protective component provided in the middle of the separation tank.

[0006] As an optional embodiment of the separation and purification device for removing endotoxins from plasmid DNA according to this utility model, wherein: the side of the connecting tube is connected to an installation tube, the end of the installation tube is inserted into the side of the extractor, the side of the extractor is connected to a feeding tube, and the side of the installation cover and the side of the separation tank are both connected to a diversion tube.

[0007] As an optional embodiment of the separation and purification device for removing endotoxins from plasmid DNA according to this utility model, wherein: a collection bucket is inserted into the side of the diversion tube, a screening box is connected to the side of the collection bucket through the diversion tube, and a collection box is connected to the side of the screening box through the diversion tube.

[0008] As an optional embodiment of the separation and purification device for removing endotoxins from plasmid DNA according to this utility model, the protective component includes a separation groove formed in the middle of the separation tank, a contact rod connected to the side of the separation groove, the contact rod being fixedly installed on the side of the sleeve block, and a limiting component being provided in the middle of the sleeve block.

[0009] As an optional embodiment of the separation and purification device for removing endotoxins from plasmid DNA according to this utility model, wherein: a connecting rod is fixedly connected to one side of the sleeve block, a fixing rod is installed on the other side of the sleeve block, and a rotating rod is hinged to the side of the connecting rod and the sleeve block.

[0010] As an optional embodiment of the separation and purification device for removing endotoxins from plasmid DNA according to this utility model, wherein: a fixing block is hinged to the end of the rotating rod, the side of the fixing block is fixedly connected to the inner wall of the separation tank, and a compression spring is sleeved on the side of the fixing rod.

[0011] As an alternative solution to the separation and purification device for removing endotoxins from plasmid DNA according to this utility model, the limiting component includes a connecting groove formed in the middle of the sleeve block, a connecting spring installed in the middle of the connecting groove, and a squeezing plate fixedly connected to the end of the connecting spring.

[0012] As an optional embodiment of the separation and purification device for removing endotoxins from plasmid DNA according to this utility model, wherein: a connecting plate is fixedly connected to the bottom of the connecting spring, the rotating rod is hinged to the connecting plate via a rotating shaft, and the end of the rotating rod is hinged to the fixed block via a rotating shaft.

[0013] This utility model has the following beneficial effects:

[0014] 1. This separation and purification device for removing endotoxins from plasmid DNA effectively separates plasmid DNA from endotoxins, improving DNA purity, through protective components installed in the separation tank, including a separation tank, contact rod, sleeve, and limiting components. The various components, such as the processing tank, separation tank, and collection tank, are connected by connecting pipes and diversion pipes, facilitating operator control of fluid flow and simplifying the operation process. The design of the fixing frame, mounting cover, and connecting pipes ensures high stability during operation, reducing operational risks. The modular design allows for easy disassembly and replacement of components, facilitating maintenance and cleaning. The protective components effectively prevent plasmid DNA loss during separation, while the limiting components and compression springs enhance operational safety.

[0015] 2. This separation and purification device for removing endotoxins from plasmid DNA, through the cooperation of components such as a feeder, a feeding tube, and a splitter, can achieve a certain degree of automation, reducing labor costs. This device is not only suitable for the separation and purification of plasmid DNA but can also be used for the purification of other biomolecules. The device has a compact design and small footprint, making it suitable for use in environments with limited space, such as laboratories. By optimizing the separation and purification process, this device can significantly improve the production efficiency of plasmid DNA. This separation and purification device has high practicality and market potential in removing endotoxins from plasmid DNA. Attached Figure Description

[0016] Figure 1 This is a three-dimensional structural diagram of the present invention.

[0017] Figure 2 This is a three-dimensional side view of the present invention.

[0018] Figure 3 This is a schematic diagram of the protective component structure of this utility model.

[0019] Figure 4 This is a schematic diagram of the limiting component structure of this utility model.

[0020] In the diagram: 110, Fixing frame; 120, Feeding pipe; 130, Processing tank; 140, Mounting cover; 150, Handle; 160, Connecting pipe; 170, Mounting pipe; 180, Feeder; 190, Separating tank; 200, Diverting pipe; 210, Collecting tank; 220, Screening box; 230, Motor; 240, Collecting box; 250, Separating trough; 260, Contact rod; 270, Sleeve block; 280, Compression spring; 290, Connecting rod; 300, Rotating rod; 310, Fixing block; 320, Fixing rod; 330, Connecting trough; 340, Connecting spring; 350, Compression plate; 360, Connecting plate; 370, Rotating shaft; 380, Rotating shaft; 390, Protective component; 400, Limiting component. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Example 1 aims to address the problem that some existing methods involve cumbersome procedures requiring professional personnel, which increases the risk to ordinary workers and raises experimental costs and time. Furthermore, some efficient methods are too costly for large-scale application. Please refer to [link to relevant documentation]. Figures 1-4 A separation and purification device for removing endotoxins from plasmid DNA includes a frame 110, a processing tank 130 mounted in the middle of the frame 110, a motor 230 mounted on the side of the processing tank 130, a mounting cover 140 mounted at the end of the processing tank 130, a handle 150 mounted on the side of the mounting cover 140, a connecting tube 160 inserted into the middle of the mounting cover 140, a separation tank 190 connected to the side of the connecting tube 160, and a protective component 390 provided in the middle of the separation tank 190.

[0023] A mounting pipe 170 is connected to the side of the connecting pipe 160. The end of the mounting pipe 170 is inserted into the side of the feeder 180. A feeding pipe 120 is inserted into the side of the feeder 180. A diversion pipe 200 is inserted into the side of the mounting cover 140 and the side of the separation tank 190. A collection tank 210 is inserted into the side of the diversion pipe 200. A screening box 220 is connected to the side of the collection tank 210 through the diversion pipe 200. A collection box 240 is connected to the side of the screening box 220 through the diversion pipe 200.

[0024] The processing tank 130 is mounted on the mounting frame 110. The motor 230 is used to drive the processing tank 130 to rotate or stir, so as to mix plasmid DNA with buffer or other reagents. When it is necessary to open the mounting cover 140, the handle 150 on the mounting cover 140 is rotated to open and close the processing tank 130. The connecting tube 160 is used to connect the processing tank 130 to the subsequent separation tank 190. The protection component 390 is located in the middle of the separation tank 190. The separation tank 250 is used to contain the mixture and promote the separation of plasmid DNA and endotoxin by physical or chemical methods. The contact rod 260 connects the separation tank 250 and the sleeve block 270. The limiting component 400 helps to fix and protect the separation process. The mounting tube 170 on the side of the connecting tube 160 is connected to the extractor 180 for drawing the mixture in the processing tank 130 into the separation tank 190.

[0025] The feed pipe 120 is used to add the mixture or reagent to the processing tank 130. The side of the mounting cover 140 and the side of the separation tank 190 are respectively connected to the diversion pipe 200, which is used to guide the separated liquid to different collection containers. The collection tank 210 is used to collect the liquid after the initial separation. The screening box 220 may be used for further screening or purification steps. Finally, the collection box 240 is used to collect the final purified product. The sleeve block 270, connecting rod 290, fixing rod 320, rotating rod 300 and fixing block 310 in the protection component 390 constitute a mechanical structure to protect the mixture in the separation tank 250 from external interference during the separation process. The design of the compression spring 280 and the compression plate 350 can provide a certain pressure to help the separation process. The design of the connecting spring 340 and the connecting plate 360 ​​allows the rotating rod 300 to rotate within a certain range, thereby dynamically adjusting the pressure in the separation tank 250 and the position of the contact rod 260. When the rotating rod 300 rotates, the fixing block 310 will move accordingly to adapt to different situations that may occur during the separation process.

[0026] In this embodiment, the protective component 390, which is installed in the separation tank 190 and includes a separation tank 250, a contact rod 260, a sleeve block 270, and a limiting component 400, can effectively separate plasmid DNA from endotoxin, improving DNA purity. The various components in the device, such as the processing tank 130, separation tank 190, and collection tank 210, are connected by a connecting pipe 160 and a diversion pipe 200, facilitating operator control of fluid flow and simplifying the operation process. The installation of the fixing frame 110, mounting cover 140, and connecting pipe 160 ensures high stability during operation, reducing operational risks. The modular design of the device makes it easy to disassemble and replace components, facilitating maintenance and cleaning. The protective component 390 effectively prevents plasmid DNA loss during separation, while the limiting component 400 and the compression spring 280 enhance operational safety.

[0027] Example 2 aims to address the shortcomings of existing methods, which may involve multiple steps, resulting in low efficiency and unsuitability for rapid production and urgent needs. This example is an improvement upon Example 1. For details, please refer to [link / reference]. Figures 1-4 The protection component 390 includes a separation groove 250 formed in the middle of the separation barrel 190, a contact rod 260 connected to the side of the separation groove 250, the contact rod 260 being fixedly installed on the side of the sleeve block 270, and a limit component 400 being provided in the middle of the sleeve block 270.

[0028] A connecting rod 290 is fixedly connected to one side of the sleeve block 270, and a fixing rod 320 is installed on the other side of the sleeve block 270. A rotating rod 300 is hinged to the side of the connecting rod 290 and the sleeve block 270. A fixing block 310 is hinged to the end of the rotating rod 300. The side of the fixing block 310 is fixedly connected to the inner wall of the separation barrel 190. A compression spring 280 is sleeved on the side of the fixing rod 320.

[0029] The limiting component 400 includes a connecting groove 330 formed in the middle of the sleeve block 270, a connecting spring 340 installed in the middle of the connecting groove 330, a pressing plate 350 fixedly connected to the end of the connecting spring 340, a connecting plate 360 ​​fixedly connected to the bottom of the connecting spring 340, a rotating rod 300 hinged to the connecting plate 360 ​​through a rotating shaft 370, and the end of the rotating rod 300 hinged to the fixed block 310 through a rotating shaft 380.

[0030] In this embodiment, the combination of components such as the feeder 180, the feeding tube 120, and the diversion tube 200 enables a certain degree of automation, reducing labor costs. This device is not only suitable for the separation and purification of plasmid DNA, but also for the purification of other biomolecules. The device has a compact design and a small footprint, making it suitable for use in environments with limited space, such as laboratories. By optimizing the separation and purification process, this device can significantly improve the production efficiency of plasmid DNA. This separation and purification device has high practicality and market prospects in removing endotoxins from plasmid DNA.

[0031] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0032] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A separation and purification apparatus for removing endotoxins from plasmid DNA, comprising a holder (110), characterized in that: A processing tank (130) is installed in the middle of the fixed frame (110). A motor (230) is installed on the side of the processing tank (130). An installation cover (140) is installed at the end of the processing tank (130). A handle (150) is installed on the side of the installation cover (140). A connecting pipe (160) is inserted into the middle of the installation cover (140). A separation tank (190) is connected to the side of the connecting pipe (160). A protective component (390) is provided in the middle of the separation tank (190).

2. The separation and purification apparatus for removing endotoxins from plasmid DNA according to claim 1, characterized in that: The connecting pipe (160) is connected to the side of the mounting pipe (170), the end of the mounting pipe (170) is inserted into the side of the material extractor (180), the side of the material extractor (180) is connected to the feeding pipe (120), and the side of the mounting cover (140) and the side of the separation bucket (190) are both connected to the diversion pipe (200).

3. The separation and purification apparatus for removing endotoxins from plasmid DNA according to claim 2, characterized in that: A collection bucket (210) is inserted into the side of the diversion pipe (200), and a screening box (220) is connected to the side of the collection bucket (210) through the diversion pipe (200). A collection box (240) is connected to the side of the screening box (220) through the diversion pipe (200).

4. The separation and purification apparatus for removing endotoxins from plasmid DNA according to claim 1, characterized in that: The protective component (390) includes a separation groove (250) formed in the middle of the separation barrel (190), a contact rod (260) is connected to the side of the separation groove (250), the side of the contact rod (260) is fixedly installed on the side of the sleeve block (270), and a limit component (400) is provided in the middle of the sleeve block (270).

5. The separation and purification apparatus for removing endotoxins from plasmid DNA according to claim 4, characterized in that: A connecting rod (290) is fixedly connected to one side of the sleeve block (270), and a fixing rod (320) is installed on the other side of the sleeve block (270). A rotating rod (300) is hinged to the side of the connecting rod (290) and the sleeve block (270).

6. The separation and purification apparatus for removing endotoxins from plasmid DNA according to claim 5, characterized in that: The end of the rotating rod (300) is hinged to a fixing block (310), the side of the fixing block (310) is fixedly connected to the inner wall of the separation barrel (190), and a compression spring (280) is sleeved on the side of the fixing rod (320).

7. The separation and purification apparatus for removing endotoxins from plasmid DNA according to claim 6, characterized in that: The limiting component (400) includes a connecting groove (330) formed in the middle of the sleeve block (270), a connecting spring (340) is installed in the middle of the connecting groove (330), and a pressing plate (350) is fixedly connected to the end of the connecting spring (340).

8. The separation and purification apparatus for removing endotoxins from plasmid DNA according to claim 7, characterized in that: The bottom of the connecting spring (340) is fixedly connected to a connecting plate (360), the rotating rod (300) is hinged to the connecting plate (360) through a rotating shaft (370), and the end of the rotating rod (300) is hinged to the fixed block (310) through a rotating shaft (380).

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