Separation and purification device for removing endotoxin in plasmid DNA

By designing the components of the separation and purification device and automating its operation, the problems of cumbersome operation and high cost in existing technologies have been solved, achieving efficient, safe and high-purity separation of plasmid DNA, which is suitable for laboratory environments.

CN223481116UActive Publication Date: 2025-10-28YU JI (SHANGHAI) BIOLOGICAL TECH CO LTD
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Patent Information

Application Number
CN202422676071.5
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-11-04
Publication Date
2025-10-28
Estimated Expiration
2034-11-04

AI Technical Summary

Technical Problem

The existing methods for removing endotoxins from plasmid DNA are cumbersome and require professional personnel to operate, which increases the risk and experimental costs for ordinary workers. In addition, efficient methods are expensive and are not suitable for large-scale applications.

Method used

A separation and purification device including components such as a fixture, a processing tank, a separation tank, and a collection tank has been designed. By protecting the components and automating the operation, the operation process is simplified, and the stability and safety of the device are improved. It is suitable for the purification of plasmid DNA and other biomacromolecules.

Benefits of technology

It simplifies the operation process, reduces operational risks, improves the purity and production efficiency of plasmid DNA, is suitable for laboratory environments, reduces labor costs, and has high practicality and market prospects.

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Abstract

The utility model relates to the technical field of separation and purification devices, and discloses a separation and purification device for removing endotoxin in plasmid DNA (Deoxyribonucleic Acid), which comprises a fixed frame, a treatment barrel arranged in the middle of the fixed frame, a motor arranged at the side part of the treatment barrel, a mounting cover arranged at the end part of the treatment barrel, a handle arranged at the side part of the mounting cover, and a connecting pipe inserted in the middle of the mounting cover, a separation barrel is connected to the side portion of the connecting pipe, a protection assembly is arranged in the middle of the separation barrel, and the protection assembly arranged in the separation barrel comprises a separation groove, a contact rod, a sleeve block and a limiting assembly, so that plasmid DNA and endotoxin can be effectively separated, the purity of DNA is improved, and the separation efficiency is improved. Components such as a treatment barrel, a separation barrel and a collection barrel in the device are connected through connecting pipes and flow dividing pipes, an operator can control the flow direction of fluid conveniently, the operation process is simplified, through arrangement of structures such as a fixing frame, a mounting cover and the connecting pipes, the whole device has high stability in the operation process, and risks in the operation process are reduced.
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