Suction printing sliding rail platform of CTC enrichment dyeing device

Abstract

The utility model relates to the technical field of medical inspection equipment, in particular to a suction printing sliding rail platform of a CTC enrichment dyeing device. Comprising a heating platform and a left sliding groove and a right sliding groove which are formed in the two sides of the heating platform, a jacking device for driving the left sliding groove and the right sliding groove to ascend and descend is arranged below the left sliding groove and the right sliding groove, and a micro-fluidic chip fixing assembly is installed between the left sliding groove and the right sliding groove in a sliding mode; the micro-fluidic chip fixing assembly is of a rectangular frame structure with pulleys arranged at four corners, and a bearing platform used for clamping a micro-fluidic chip is arranged in a frame of the micro-fluidic chip fixing assembly. The platform is high in automation degree, high in cell enrichment and dyeing operation efficiency and particularly suitable for cell enrichment and dyeing operation of batch CTC chips, and the CTC detection efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of medical testing equipment technology, specifically to a suction slide platform for a CTC enrichment staining device. Background Technology

[0002] CTC, or circulating tumor cell detection, is a newly emerging tumor diagnostic technology that has gained popularity in recent years. Studies have shown that circulating tumor cells can be detected in peripheral blood before solid tumors form, making CTC detection highly suitable for early screening and diagnosis of malignant tumors. Furthermore, CTC detection has shown good results in prognosis, disease progression monitoring, recurrence prediction, postoperative monitoring of small lesions in malignant tumors, and the design and monitoring of targeted drug therapy effects, making it an advanced method for early screening and diagnosis of malignant tumors. However, due to the very low concentration of circulating tumor cells in peripheral blood, the capture and identification of circulating tumor cells in CTC detection presents a significant challenge. Another Chinese patent of the applicant, 2019103326886, discloses an integrated reaction device and method for the enrichment and staining of nucleated cells in body fluids by blotting, authorized publication number CN111855333B. This reaction device includes a microporous membrane with a fixing component, a reaction tank on the fixing component, a top cover on the reaction tank, a liftable platform on one side of the fixing component, and a storage chamber below the fixing component; the storage chamber is filled with an absorbent material. When the cell suspension passes through the microporous membrane, cells larger than the membrane pore size are retained in the reaction vessel and adsorbed onto the membrane surface due to the suction filtration and retention method. Because the water-absorbing substance has a stable suction rate, gentle attraction force, and is slow and essentially pressureless, the cells adhere to the membrane surface. This achieves the capture and enrichment of malignant cells and pathogens in body fluids. The microporous membrane in this device, also called a microfluidic chip, is a key component for enriching circulating tumor cells. Existing microporous membranes all use flexible fiber filter membranes or filter paper as filter media. For example, another Chinese patent of the applicant, 202321916277X, discloses "A Microfluidic Device and Integrated Cell Enrichment and Staining Equipment," with authorization announcement number CN220371064U, in which the microfluidic device includes: a liquid accumulation platform, a filter assembly, and a platform mesh. The liquid collection platform has a funnel-shaped internal structure with guide strips protruding from its surface for guiding the filtrate. The filtration assembly is a microfiltration membrane located above the liquid collection platform for filtering the liquid sample. During CTC testing, blood samples and test reagents need to be added to the microfluidic device one by one, which is cumbersome and inefficient. Utility Model Content

[0003] To address the problems of the prior art, this invention provides an aspiration slide platform for a CTC enrichment staining device, used for batch CTC chip cell enrichment staining operations, thereby improving CTC detection efficiency.

[0004] To achieve the above objectives, this utility model provides the following technical solution:

[0005] The CTC enrichment and staining device of this utility model includes a suction slide platform, a heating platform, and a left slide and a right slide on both sides of the heating platform. A lifting device for driving the left slide and the right slide is provided below them. A microfluidic chip fixing assembly is slidably installed between the left slide and the right slide.

[0006] The above structural design allows multiple microfluidic chips to be placed on a fixed assembly to receive reagents simultaneously, and multiple microfluidic chips can be heated at the same time. This is beneficial for the cell enrichment and staining of batch CTC chips and improves the efficiency of CTC detection.

[0007] Preferably, the microfluidic chip fixing assembly is a rectangular frame structure with pulleys at the four corners. The pulleys can slide freely in the left and right sliding grooves. The frame of the microfluidic chip fixing assembly is provided with a support for holding the microfluidic chip.

[0008] With the above structural design, the pulley drives the microfluidic chip fixing component to slide along the left and right slide grooves.

[0009] Preferably, the pulley is connected to the microfluidic chip fixing assembly via an extension rod perpendicular to the left or right slide groove, and an extension rod lifting device is provided on the outside of the heating platform corresponding to the position of the extension rod.

[0010] With the above structural design, after adding reagents to the microfluidic chip, the microfluidic chip fixing component can be tilted by raising one side of the microfluidic chip fixing component, which is conducive to the rapid discharge of excess reagents.

[0011] Preferably, the support is a folded edge disposed inside the frame of the microfluidic chip fixing component.

[0012] With the above structural design, the microfluidic chip fixing component has a simple structure and facilitates the placement and removal of the microfluidic chip.

[0013] Preferably, the front ends of the left slide and the right slide have extension sections that extend beyond the front side of the heating platform, and the length of the extension section is not less than the width of the microfluidic chip fixing assembly.

[0014] With the above structural design, the absorbent material at the bottom of the microfluidic chip can be peeled off after enrichment by moving the microfluidic chip fixing component, which facilitates subsequent operations.

[0015] Preferably, the lifting device is disposed at both ends of the left slide groove and the right slide groove.

[0016] With the above structural design, the lifting devices set at both ends of the left and right slides keep the left and right slides in a horizontal state and raise and lower synchronously, thereby smoothly lifting the microfluidic chip fixing component and preventing reagents from spilling out of the microfluidic chip fixing component during movement.

[0017] Compared with the prior art, the beneficial effects of this utility model are:

[0018] This device can simultaneously place multiple microfluidic chips on a fixed assembly to receive reagents, and can simultaneously heat multiple microfluidic chips, which is beneficial for the cell enrichment and staining of batch CTC chips and improves the efficiency of CTC detection. Attached Figure Description

[0019] Figure 1 This is a three-dimensional structural diagram of one embodiment of the present invention.

[0020] Figure 2 This is a three-dimensional structural diagram of the microfluidic chip fixing assembly.

[0021] Figure 3 This is a cross-sectional view of a microfluidic chip. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] This invention relates to a CTC enrichment staining device with an aspiration slide platform comprising a heating platform 4 and a left slide 1 and a right slide 2 disposed on both sides of the heating platform 4. The left slide 1 and right slide 2 are used to support the microfluidic chip 5 for enrichment staining. The microfluidic chip 5, also called a microporous membrane, is a key component for enriching circulating tumor cells. To quickly remove residual reagents after enrichment staining, a liquid aspiration component 7 is usually attached to the back of the microfluidic chip 5 to absorb and store residual liquid passing through the microfluidic chip 5. After cell enrichment is completed, the liquid aspiration component 7 needs to be separated from the microfluidic chip 5, and the microfluidic chip 5 is extracted for the next detection step.

[0024] Below the left slide 1 and right slide 2, a lifting device 3 is installed to drive their lifting and lowering. To maintain balance, one lifting device 3 is installed at each end of the left slide 1 and right slide 2. In use, the four lifting devices 3 operate synchronously, keeping the left slide 1 and right slide 2 in a horizontal state and lifting and lowering synchronously. The lifting device 3 can be a hydraulic cylinder or a pneumatic cylinder.

[0025] A microfluidic chip fixing assembly 6 is slidably installed between the left slide groove 1 and the right slide groove 2. For example... Figure 2 As shown, the microfluidic chip fixing assembly 6 is a rectangular frame structure with pulleys 601 at its four corners. The pulleys 601 are connected to the microfluidic chip fixing assembly 6 via extension rods 603 perpendicular to the left slide groove 1 or the right slide groove 2. An extension rod lifting device 8 is provided on the outside of the heating platform 4 corresponding to the position of the extension rod 603. This extension rod lifting device 8 can be a hydraulic cylinder or a pneumatic cylinder. Inside the frame of the microfluidic chip fixing assembly 6, there is a support platform 602 for holding the microfluidic chip 5. The support platform 602 is either a folded edge or a boss located inside the frame of the microfluidic chip fixing assembly 6. When the microfluidic chip 5 is placed inside the frame of the microfluidic chip fixing assembly 6, the folded edge or the boss can support the edge of the microfluidic chip 5, preventing it from falling out of the frame.

[0026] Furthermore, the front ends of the left slide 1 and the right slide 2 have extension sections 9 that extend beyond the front side of the heating platform 4, and the length of the extension sections 9 is not less than the width of the microfluidic chip fixing assembly 6. That is, the length of the left slide 1 and the right slide 2 exceeds the width of at least one microfluidic chip fixing assembly 6 of the heating platform 4.

[0027] In operation, the lifting device 3, left slide 1, and right slide 2 are initially in a low position. The microfluidic chip fixing assembly 6 is placed at the front end of the left slide 1 and right slide 2, which is also the front end of the heating platform 4. At this time, the height of the microfluidic chip 5 placed on the microfluidic chip fixing assembly 6 is slightly higher than the upper surface of the heating platform 4, but the position of the liquid absorption assembly 7, which is snapped under the microfluidic chip 5, is lower than the upper surface of the heating platform 4. During the enrichment process, after the microfluidic chip 5 is filled with reagent, the excess waste reagent passes through the microfluidic chip 5 and collects in the liquid absorption assembly 7. At this time, the microfluidic chip fixing assembly 6 is pushed towards the heating platform 4, and the microfluidic chip 5 moves to the top of the heating platform 4 along with the microfluidic chip fixing assembly 6. The liquid absorption assembly 7 is blocked by the heating platform 4 and cannot move, so it will fall off from under the microfluidic chip 5, thus separating the liquid absorption assembly 7 from the microfluidic chip 5. The power to push the microfluidic chip fixing assembly 6 can be manual or an automatically controlled mechanical device. After the microfluidic chip fixing assembly 6 is moved above the heating platform 4, an extension rod 603 on its side moves directly above the extension rod lifting device 8. Cell staining reagent is added into the microfluidic chip 5. Subsequently, the heating platform 4 operates to raise the temperature of the reagent in the microfluidic chip 5, maintaining its high reactivity. After cell staining is complete, the extension rod lifting device 8 is activated to raise one end of the microfluidic chip fixing assembly 6, tilting the device and removing the reagent from the microfluidic chip 5. After reagent removal, the lifting device 3 is raised, raising the positions of the left slide 1, right slide 2, and microfluidic chip fixing assembly 6, detaching them from the upper surface of the heating platform 4. After staining is complete, the microfluidic chip 5 can be removed for microscopic examination.

[0028] Contents not described in detail in this specification are existing technologies known to those skilled in the art. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A CTC enrichment dyeing apparatus with a suction slide platform, comprising a heating platform (4) and a left slide groove (1) and a right slide groove (2) disposed on both sides of the heating platform (4), characterized in that: A lifting device (3) for driving the left slide (1) and the right slide (2) is provided below them, and a microfluidic chip fixing assembly (6) is slidably installed between the left slide (1) and the right slide (2).

2. The suction slide platform of the CTC enrichment dyeing apparatus according to claim 1, characterized in that: The microfluidic chip fixing assembly (6) is a rectangular frame structure with pulleys (601) at the four corners. The pulleys (601) can slide freely in the left slide groove (1) and the right slide groove (2).

3. The suction slide platform of the CTC enrichment dyeing apparatus according to claim 1 or 2, characterized in that: The microfluidic chip fixing assembly (6) has a support (602) inside its frame for securing the microfluidic chip (5).

4. The suction slide platform of the CTC enrichment dyeing apparatus according to claim 2, characterized in that: The pulley (601) is connected to the microfluidic chip fixing assembly (6) via an extension rod (603) perpendicular to the left slide groove (1) or the right slide groove (2). An extension rod lifting device (8) is provided on the outside of the heating platform (4) at a position corresponding to the extension rod (603).

5. The suction slide platform of the CTC enrichment dyeing apparatus according to claim 3, characterized in that: The support (602) is a folded edge set inside the frame of the microfluidic chip fixing assembly (6).

6. The suction slide platform of the CTC enrichment dyeing apparatus according to claim 1, characterized in that: The front ends of the left slide (1) and the right slide (2) have extensions (9) that extend beyond the front side of the heating platform (4).

7. The suction slide platform of the CTC enrichment dyeing apparatus according to claim 6, characterized in that: The length of the extension section (9) is not less than the width of the microfluidic chip fixing component (6).

8. The suction slide platform of the CTC enrichment dyeing apparatus according to claim 1, characterized in that: The lifting device (3) is located at both ends of the left slide (1) and the right slide (2).

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