Porous membrane wall type PCR reaction tube and nucleic acid POCT detector
By designing a multi-porous membrane-walled PCR reaction tube and a nucleic acid POCT detector, the simultaneous detection of nucleic acids from multiple pathogens was achieved, solving the problem of excessively long detection time in existing technologies and improving detection efficiency and accuracy.
Patent Information
- Application Number
- CN202422694779.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-05
- Publication Date
- 2025-10-31
- Estimated Expiration
- 2034-11-05
AI Technical Summary
Existing PCR reaction tubes and nucleic acid POCT detectors can only detect one pathogen at a time, resulting in excessively long nucleic acid testing times and failing to meet the needs for early diagnosis of multiple pathogens.
A multi-well membrane-walled PCR reaction tube is designed, comprising a container body and a light-transmitting element. The container body is provided with an inlet, a notch, and a flow channel. The light-transmitting element forms multiple detection wells. Combined with the detection slot of a nucleic acid POCT detector, each well corresponds one-to-one with the detection light channel, enabling the simultaneous detection of nucleic acids of multiple pathogens.
It significantly improves detection efficiency, shortens the time for pathogen nucleic acid detection, enhances convenience and accuracy of test results, is suitable for robotic arm operation, and shortens the diagnostic cycle.
Smart Images
Figure CN223500880U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of pathogen nucleic acid detection technology, and in particular to a multi-porous membrane-walled PCR reaction tube and a nucleic acid POCT detector. Background Technology
[0002] There are many types of pathogens that can cause infection, making early diagnosis impossible through clinical symptoms alone. Pathogen testing is the most direct basis for early diagnosis of infectious diseases.
[0003] Nucleic acid testing is more sensitive and specific than marker detection such as antigens or antibodies in early diagnosis. Pathogen nucleic acid testing can directly detect the genetic material of pathogens (such as viral or bacterial DNA or RNA), accurately confirming the presence of infection, especially in the early stages. In contrast, antigen and antibody testing may take time because it takes time for the immune system to produce an antibody response to an antigen after infection, and antibody testing detects the body's immune response to pathogens; therefore, it may not provide accurate diagnostic results in the early stages of infection.
[0004] Existing PCR reaction tubes consist of a tube body and a cap. The tube body has a cavity and a well connected to the cavity. During fluorescence detection, the nucleic acid POCT detector detects and analyzes the nucleic acid in the sample to be tested by emitting light into the well.
[0005] However, due to the wide variety of pathogens that can cause infection, the above-mentioned detection methods can only detect one pathogen per test, and multiple tests are often required to obtain an accurate diagnostic result, which leads to an excessively long overall time for nucleic acid testing. Utility Model Content
[0006] The main purpose of this invention is to provide a multi-well membrane-walled PCR reaction tube and a nucleic acid POCT detector, which aims to meet the requirements of multi-well membrane-walled PCR reaction tubes for simultaneously performing nucleic acid detection of multiple pathogens, shorten the nucleic acid detection time, and thus shorten the diagnostic cycle.
[0007] To achieve the above objectives, this utility model proposes a porous membrane-walled PCR reaction tube, comprising:
[0008] The container body includes a liquid inlet, at least two notches, and a flow channel connecting the liquid inlet and the at least two notches; and
[0009] A light-transmitting element is provided at the notch of the container body, and together with the notch, forms at least two detection holes.
[0010] Optionally, the notches are all opened at the edge of the container body away from the liquid inlet and are evenly spaced.
[0011] Optionally, the light-transmitting element is in the form of a U-shaped strip.
[0012] Optionally, the light-transmitting element and the container body are injection molded; or
[0013] The light-transmitting element is snapped onto the container body; or
[0014] The light-transmitting element is adhered to the container body; or
[0015] The light-transmitting element is fused to the container body.
[0016] Optionally, the porous membrane-walled PCR reaction tube further includes a cap screwed onto the inlet of the container body.
[0017] Optionally, the container body includes a circular tube and a flow divider plate integrally formed with the circular tube, the liquid inlet is located at one end of the circular tube, and the flow channel and the notch are located on the flow divider plate.
[0018] Optionally, the circular tube is provided with a protrusion extending radially thereon.
[0019] To achieve the above objectives, this utility model also proposes a nucleic acid POCT detection instrument, including a detection stage and a detection slot disposed on the detection stage. The detection slot is used to accommodate the multi-porous membrane-walled PCR reaction tubes as described above. The multi-porous membrane-walled PCR reaction tubes include:
[0020] The container body includes a liquid inlet, at least two notches, and a flow channel connecting the liquid inlet and the at least two notches; and
[0021] A light-transmitting element is provided at the notch of the container body, and together with the notch, forms at least two detection holes.
[0022] Optionally, the notches are all opened at the edge of the container body away from the liquid inlet and are evenly spaced.
[0023] Optionally, the detection slot is provided with a detection light channel that is one-to-one with the detection well position of the multi-porous membrane PCR reaction tube.
[0024] Optionally, the top cover of the detection slot is provided with a movable light-shielding element.
[0025] In the technical solution of this utility model, the multi-porous membrane-walled PCR reaction tube includes a container body and a light-transmitting element. The container body has an inlet, at least two notches, and a flow channel connecting the inlet and the at least two notches. The light-transmitting element is located at the notches of the container body and forms at least two detection wells with the notches. It is understood that this utility model provides a multi-porous membrane-walled PCR reaction tube with a significantly higher heat transfer rate than traditional PCR reaction tubes. It has multiple detection wells, which can meet the requirements of nucleic acid POCT detectors for simultaneously performing nucleic acid detection of multiple pathogens. Each detection well is set one-to-one with the detection light channel of the detector. During fluorescence detection, light passes through the light-transmitting element and shines onto the nucleic acid of the sample to be tested in the detection well. It can simultaneously perform nucleic acid detection of 1 to 21 pathogens, greatly improving detection efficiency, significantly shortening the time for pathogen nucleic acid detection, and is suitable for robotic arm operation, significantly improving convenience and greatly shortening the diagnostic cycle. Attached Figure Description
[0026] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.
[0027] Figure 1 This is a schematic diagram of the structure of an embodiment of the porous membrane-walled PCR reaction tube of this utility model;
[0028] Figure 2 This is a schematic diagram of the structure of an embodiment of the nucleic acid POCT detection instrument of this utility model.
[0029] Explanation of icon numbers:
[0030] 100. Multi-well membrane-walled PCR reaction tube; 10. Container body; 20. Light-transmitting component; 30. Cap; 10a. Notch; 10b. Flow channel; 101. Detection well; 11. Circular tube; 12. Flow divider; 111. Protruding column; 200. Detection stage; 200a. Detection groove; 300. Light-shielding component.
[0031] The realization of the purpose, functional features and advantages of this utility model will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation
[0032] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0033] It should be noted that if the embodiments of this utility model involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.
[0034] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.
[0035] Furthermore, if the embodiments of this utility model involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, features defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, if the word "and / or" appears throughout the text, it means including three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution that simultaneously satisfies A and B. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.
[0036] This invention proposes a porous membrane-walled PCR reaction tube, which can be applied to nucleic acid POCT detection instruments, etc., and is not limited to this.
[0037] Reference Figure 1 and Figure 2In one embodiment of the present invention, the porous membrane-walled PCR reaction tube 100 includes a container body 10 and a light-transmitting element 20. The container body 10 is provided with an inlet, at least two notches 10a, and a flow channel 10b connecting the inlet and the at least two notches 10a. The light-transmitting element 20 is disposed at the notch 10a of the container body 10 and surrounds the notch 10a to form at least two detection wells 101.
[0038] In this embodiment, the porous membrane-walled PCR reaction tube 100 may further include a cap 30, which is screwed onto the liquid inlet of the container body 10. Both the container body 10 and the cap 30 may be made of medical-grade plastic material, and the light-transmitting element 20 may be made of medical-grade PPMA, PC, etc., which is not limited here.
[0039] In this embodiment, there are preferably seven notches 10a, which can simultaneously meet the needs of nucleic acid detection for 21 pathogens. The notches 10a can be opened at the edge of the container body 10 away from the liquid inlet and evenly spaced.
[0040] During manufacturing, the light-transmitting element 20 and the container body 10 can be injection molded, the light-transmitting element 20 can be snapped onto the container body 10, the light-transmitting element 20 can be glued onto the container body 10, or the light-transmitting element 20 can be fused onto the container body 10, which is not limited here.
[0041] It is understood that this utility model provides a porous membrane-walled PCR reaction tube 100, which has a significantly higher heat transfer rate than traditional PCR reaction tubes. It has multiple detection wells 101, which can meet the requirements of nucleic acid POCT detectors to simultaneously perform nucleic acid detection of multiple pathogens. Each detection well 101 is set one-to-one with the detection light channel of the detector. During fluorescence detection, the light passes through the light-transmitting element 20 and shines on the nucleic acid of the sample to be tested in the detection well 101. It can simultaneously perform nucleic acid detection of 1 to 21 pathogens, greatly improving detection efficiency, significantly shortening the time for pathogen nucleic acid detection, and is suitable for robotic arm operation, significantly improving convenience and greatly shortening the diagnostic cycle.
[0042] To save materials and facilitate processing, while simultaneously meeting the needs for nucleic acid detection of multiple pathogens, refer to Figure 1 In one embodiment, the container body 10 may include a circular tube 11 and a flow divider 12 integrally formed with the circular tube 11. The liquid inlet is located at one end of the circular tube 11, and the flow channel 10b and the notch 10a are located on the flow divider 12.
[0043] In this embodiment, the light-transmitting element 20 can be U-shaped to simultaneously surround all the gaps 10a, facilitating assembly and providing good sealing. Of course, in some other embodiments, multiple light-transmitting elements can be used to seal the multiple gaps 10a on the edge of the diverter plate 12 respectively; the number and shape of the light-transmitting elements 20 are not limited here.
[0044] In addition, to improve the stability of the electric gripper of the nucleic acid POCT detector in holding the multi-porous membrane-walled PCR reaction tube 100, refer to Figure 1 and Figure 2 Based on the above embodiments, the circular tube 11 is provided with a protrusion 111 extending radially thereon. When the electric clamp is holding the tube, the protrusion 111 of the porous membrane PCR reaction tube 100 is inserted into the corresponding insertion hole of the electric clamp.
[0045] This invention also proposes a nucleic acid POCT detection instrument, which includes a detection stage 200 and a detection slot 200a disposed on the detection stage 200. The detection slot 200a is used to accommodate the aforementioned porous membrane-walled PCR reaction tube 100. The specific structure of the porous membrane-walled PCR reaction tube 100 is as described in the above embodiments. Since the nucleic acid POCT detection instrument proposed in this invention includes all schemes of all embodiments of the aforementioned porous membrane-walled PCR reaction tube 100, it has at least the same technical effects as the aforementioned porous membrane-walled PCR reaction tube 100, which will not be described in detail here.
[0046] Reference Figure 1 and Figure 2 In this embodiment, a detection optical channel is provided in the detection slot 200a at the detection well position 101 corresponding to the multi-well membrane-walled PCR reaction tube 100, and is configured one-to-one with it. This configuration can simultaneously complete the detection of 1 to 21 pathogen nucleic acids, greatly improving detection efficiency, significantly shortening the detection time of pathogen nucleic acid, significantly improving convenience, and ensuring the accuracy of detection results.
[0047] To further improve the accuracy of test results, refer to Figure 1 and Figure 2 In one embodiment, the top cover of the detection slot 200a is provided with a movable light-shielding member 300. During detection, the light-shielding member 300 can be moved by a person or by the grippers of the detection instrument.
[0048] The above description is only an optional embodiment of the present utility model and does not limit the patent scope of the present utility model. All equivalent structural transformations made under the inventive concept of the present utility model using the contents of the present utility model specification and drawings, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present utility model.
Claims
1. A porous membrane-walled PCR reaction tube, characterized in that, include: The container body is provided with a liquid inlet, at least two notches, and a flow channel connecting the liquid inlet and the at least two notches; as well as A light-transmitting element is provided at the notch of the container body, and together with the notch, forms at least two detection holes.
2. The porous membrane-walled PCR reaction tube as described in claim 1, characterized in that, The notches are all located on the edge of the container body away from the liquid inlet and are evenly spaced.
3. The porous membrane-walled PCR reaction tube as described in claim 2, characterized in that, The light-transmitting element is U-shaped.
4. The porous membrane-walled PCR reaction tube as described in claim 1, characterized in that, The light-transmitting component and the container body are formed by injection molding; or The light-transmitting element is snapped onto the container body; or The light-transmitting element is adhered to the container body; or The light-transmitting element is fused to the container body.
5. The porous membrane-walled PCR reaction tube as described in claim 1, characterized in that, The porous membrane-walled PCR reaction tube also includes a cap, which is screwed onto the inlet of the container body.
6. The porous membrane-walled PCR reaction tube as described in claim 1, characterized in that, The container body includes a circular tube and a flow divider plate integrally formed with the circular tube. The liquid inlet is located at one end of the circular tube, and the flow channel and the notch are located on the flow divider plate.
7. The porous membrane-walled PCR reaction tube as described in claim 6, characterized in that, The circular tube is provided with protruding posts that extend radially therefrom.
8. A nucleic acid POCT detection instrument, characterized in that, The device includes a detection stage and a detection slot disposed on the detection stage, the detection slot being used to accommodate a porous membrane-walled PCR reaction tube as described in any one of claims 1 to 7.
9. The nucleic acid POCT detection instrument as described in claim 8, characterized in that, The detection slot is equipped with a detection light channel that is one-to-one with the detection well position of the multi-porous membrane PCR reaction tube.
10. The nucleic acid POCT detection instrument as described in claim 8, characterized in that, The top cover of the detection slot is equipped with a movable light-shielding component.