Gene amplification and gene detection all-in-one machine

Abstract

The utility model discloses a gene amplification and gene detection all-in-one machine which comprises a machine shell, an electrophoresis module arranged on the end face of the machine shell, a gene amplification module embedded in the end face of the machine shell, and a power supply module for supplying power to the electrophoresis module and the gene amplification module, and the power supply module is arranged in the machine shell; the electrophoresis module comprises an electrophoresis shell, an electrophoresis tank located in the electrophoresis shell and electrode bars installed at the two ends of the electrophoresis tank. The gene amplification module comprises a reaction plate and a thermoelectric module; a temperature control hole array is arranged in the middle of the reaction plate; and the thermoelectric module is used for providing cooling and heating functions for the reaction plate. According to the structure, the electrophoresis module, the gene amplification module and the centrifugal module are integrated on the shell and are centrally powered by the power supply module, so that the space of a laboratory is saved, and the experiment efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of biogene technology, and in particular to an integrated machine for gene amplification and gene detection. Background Technology

[0002] Currently, gene amplification instruments and gene detection instruments are usually separate devices in laboratories. This not only occupies a large amount of laboratory space, but also requires separate operation of different devices during the experiment, increasing the complexity and time cost. In addition, each device requires its own power supply system and operating space, which brings certain difficulties to the layout and management of the laboratory.

[0003] Moreover, before the gene amplification reaction, it is often necessary to use centrifugation to gather the liquid sample remaining on the wall of the reaction tube to the bottom of the reaction tube to ensure that the components in the reaction system are fully in contact and that the volume of the reaction is consistent with the preset volume, thereby improving the accuracy of the experiment; therefore, centrifuges are also an essential instrument, and the layout of centrifuges further increases the space cost of the laboratory. Utility Model Content

[0004] To address the aforementioned technical problems, this utility model provides an integrated gene amplification and gene detection machine that integrates an electrophoresis module, a gene amplification module, and a centrifugation module onto a single casing, saving laboratory space and improving experimental efficiency.

[0005] The technical solution of this utility model is:

[0006] The device includes a housing, an electrophoresis module disposed on the end face of the housing, a gene amplification module embedded on the end face of the housing, and a power supply module that provides power to the electrophoresis module and the gene amplification module, the power supply module being disposed inside the housing; the electrophoresis module includes an electrophoresis shell, an electrophoresis tank located inside the electrophoresis shell, and electrode rods installed at both ends of the electrophoresis tank; the gene amplification module includes a reaction plate with a temperature control hole array in the middle, and a thermoelectric module that provides cooling and heating functions to the reaction plate.

[0007] Furthermore, the end face of the housing is also fitted with a centrifugal module, which includes a centrifugal assembly. The centrifugal assembly includes a centrifugal mounting frame fixed inside the housing, a rotor for placing the reaction tube, and a centrifugal motor for driving the rotor and the reaction tube to rotate axially. The centrifugal motor is fixed inside the centrifugal mounting frame, and the rotor is connected to the motor shaft of the centrifugal motor through a rotor seat.

[0008] Furthermore, the gene amplification module also includes a heat dissipation module disposed below the reaction plate. The heat dissipation module includes a heat dissipation module with multiple heat dissipation fins and a fan assembly for accelerating the heat flow between the heat dissipation fins.

[0009] Furthermore, the gene amplification module also includes an amplification flap, which is hinged to the housing, and the housing is also provided with a support liner that cooperates with the amplification flap.

[0010] Furthermore: A hot cover plate and a heating circuit board are sequentially arranged above the reaction plate. The hot cover plate and the heating circuit board are fixed to each other by screws. The hot cover plate covers the reaction tube in the temperature control hole.

[0011] Furthermore, the reaction plate is provided with an annular pressure plate, which is tightly fitted to the circumferential surface of the reaction plate.

[0012] Furthermore, an annular sealing ring is provided between the annular pressure plate and the hot-press cover plate.

[0013] Furthermore, the centrifuge module also includes a centrifuge flip cover, which is hinged to the housing. The housing is also provided with a positioning liner that cooperates with the centrifuge flip cover. The inner wall of the centrifuge flip cover is provided with a number of pin protrusions, which cooperate with a number of pin holes on the positioning liner.

[0014] Furthermore: the positioning liner is also provided with a hollow cylindrical shell in the middle, the rotor rotates in the cylindrical shell, the upper part of the cylindrical shell is provided with an end cap, the end cap is made of transparent material, and the centrifuge flip cover is provided with a viewing window.

[0015] Furthermore, the electrophoresis module also includes a blue light component disposed below the electrophoresis tank. The blue light component includes a blue light fixing groove fixed below the end face of the housing. A light-emitting plate is provided in the mounting cavity of the blue light fixing groove, and the light source is disposed on the light-emitting plate. The end face of the housing opposite the light-emitting plate has an opening. Both the electrophoresis shell and the electrophoresis tank are made of transparent material.

[0016] The beneficial technical effects of this utility model are as follows: by integrating the gene amplification module, electrophoresis module and power supply module into one housing, the equipment structure is compact, saving laboratory space; the ease of operation of the equipment is improved; the structure also creatively integrates the blue light component and centrifugation module into the housing, realizing further integration of the equipment and efficient use of space, bringing convenience and benefits to laboratory work. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall external structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the electrophoresis assembly structure of this utility model;

[0019] Figure 3 This is a schematic diagram of the blue light component structure of this utility model;

[0020] Figure 4 This is a schematic diagram of the mounting hole structure of this utility model;

[0021] Figure 5 This is a schematic diagram of the centrifugal assembly structure of this utility model;

[0022] Figure 6 This is a schematic diagram of the overall front structure of this utility model;

[0023] Figure 7 This is a schematic diagram of the positioning liner structure of this utility model;

[0024] Figure 8 This is a schematic diagram of the internal structure of the gene amplification module of this utility model;

[0025] Figure 9 This is a utility model Figure 6 Overall AA sectional view;

[0026] Figure 10 This is a schematic diagram of the internal exploded structure of the gene amplification module of this utility model;

[0027] in:

[0028] 1. Housing; 11. Mounting hole; 12. Centrifugal through hole; 13. Air outlet; 2. Power module;

[0029] 3. Electrophoresis module; 31. Electrophoresis assembly; 311. Electrophoresis shell; 312. Electrophoresis tank; 313. Electrode tank; 314. Mounting part; 315. Banana plug; 32. Blue light assembly; 321. Blue light fixing tank; 322. Light-emitting plate; 323. Blue filter plate;

[0030] 4. Centrifuge module; 41. Centrifuge assembly; 411. Centrifuge mounting bracket; 412. Rotor; 413. Centrifuge motor; 414. Motor cover; 415. Elastic sleeve; 42. Centrifuge flip cover; 43. Opening / closing button; 44. Positioning liner; 441. Pin hole; 442. Cylindrical outer shell; 443. Reinforcing block; 444. End cap;

[0031] 5. Gene amplification module; 51. Reaction module; 511. Reaction plate; 512. Annular pressure plate; 513. Annular sealing ring; 514. Peltier device; 515. Heated cover pressure plate; 5151. Circumferential guide surface; 516. Heating circuit board; 517. Annular groove plate; 5171. Annular groove; 52. Heat dissipation module; 521. Heat dissipation module; 522. Fan assembly; 53. Amplification flip cover; 531. Positioning pin; 54. Support liner. Detailed Implementation

[0032] In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the specific embodiments of this utility model will be further described in detail below with reference to the accompanying drawings and examples. The following examples are used to illustrate this utility model, but are not intended to limit the scope of this utility model.

[0033] like Figure 1 As shown, the gene amplification and gene detection integrated machine of this utility model includes a housing 1, a power module 2 disposed within the housing 1, an electrophoresis module 3, a centrifugation module 4, and a gene amplification module 5 embedded in the end face of the housing 1. The power module 2 provides power to the electrophoresis module 3, the centrifugation module 4, and the gene amplification module 5 respectively; the centrifugation module 4 allows residual liquid samples on the reaction tube wall to gather to the bottom of the reaction tube under centrifugal force; the gene amplification module 5 can amplify DNA or RNA sequences from the liquid samples in the reaction tube; and the electrophoresis module 3 is used to perform electrophoresis on the liquid samples after gene amplification.

[0034] like Figures 2 to 3 As shown, the electrophoresis module 3 includes an electrophoresis assembly 31 and a blue light assembly 32 disposed below the electrophoresis assembly 31. The electrophoresis assembly 31 is located on the end face of the housing 1, and the blue light assembly 32 is located inside the housing 1. The blue light assembly 32 facilitates the monitoring of the entire electrophoresis process. The electrophoresis assembly 31 includes an electrophoresis shell 311 and an electrophoresis tank 312 located inside the electrophoresis shell 311 for electrophoresis. The bottom of the two inner sides of the electrophoresis tank 312 is respectively provided with recessed electrode grooves 313. The electrode grooves 313 on both sides are parallel to each other, and the electrode rods for electrophoresis are respectively accommodated in the electrode grooves 313 on both sides.

[0035] The electrode rods in the electrode slots 313 on both sides are respectively connected to the electrode output terminals of the power module 2. Specifically, banana plugs 315 are provided on the outside of the two sets of electrode slots 313, and the two sets of electrode rods are respectively plugged into the corresponding power interfaces through the banana plugs 315.

[0036] Because platinum wire has high chemical stability as an electrode, this embodiment uses platinum wire electrode rods to ensure the accuracy of electrophoretic separation. To prevent the platinum wire electrode rods from bending and breaking during use, a mounting part 314 is provided along the extension direction of the electrode groove 313. The platinum wire electrode rods are placed through the mounting part 314 to avoid mechanical damage.

[0037] The blue light component 32 includes a blue light fixing groove 321 that is fixed to the lower end face of the housing 1 with screws. A light-emitting plate 322 is provided in the mounting cavity of the blue light fixing groove 321, and the light source is set on the light-emitting plate 322. In this embodiment, the light source is blue light. A blue filter plate 323 is also provided on the light-emitting plate 322. After the stray light is filtered by the blue filter plate 323, the blue light is concentrated and enhanced for easy observation. At the same time, both the electrophoresis shell 311 and the electrophoresis tank 312 are made of transparent material.

[0038] like Figures 4 to 5 The centrifuge module 4 includes a centrifuge assembly 41. A mounting hole 11 is provided on the housing 1 at the location of the centrifuge module 4. The bottom of the mounting hole 11 has a centrifuge through hole 12 for the centrifuge assembly 41 to pass through and be fixed. The centrifuge assembly 41 includes a centrifuge mounting frame 411, a rotor 412 for placing reaction tubes, and a centrifuge motor 413 that drives the rotor 412 and reaction tubes to rotate axially along the centrifuge through hole 12. Specifically, the rotor 412 is connected to the motor shaft of the centrifuge motor 413 via a rotor 412 seat. Screw holes are provided around the centrifuge through hole 12. The centrifuge mounting frame 411 is fixed below the centrifuge through hole 12 by screws, and the centrifuge motor 413 is fixed inside the centrifuge mounting frame 411.

[0039] In order to avoid the rotor 412 from vibrating violently during high-speed rotation, in this embodiment, the motor cover 414 on the upper part of the centrifugal motor 413 and the lower part of the centrifugal motor 413 are respectively connected to the centrifugal fixing frame 411 through elastic fittings 415.

[0040] Furthermore, such as Figures 6 to 7 The centrifuge module 4 also includes a centrifuge cover 42 with a viewing window. The centrifuge cover 42 is hinged to the housing 1 and can be locked and opened by an opening / closing button 43. The housing 1 is also provided with a positioning liner 44 that cooperates with the centrifuge cover 42 to ensure that the centrifuge cover 42 is in the correct position when closed, and can also support the weight of the centrifuge cover 42. Specifically, the inner wall of the centrifuge cover 42 is provided with several pin protrusions, which cooperate one by one with several pin holes 441 on the positioning liner 44 to prevent the centrifuge cover 42 from shaking or misaligning after closing.

[0041] Furthermore, the positioning liner 44 also has a hollow cylindrical outer shell 442 in its center. The rotor 412 rotates inside the cylindrical outer shell 442. The outer peripheral wall of the cylindrical outer shell 442 is integrally reinforced with the positioning liner 44 by multiple reinforcing blocks 443 to improve the stability of the cylindrical outer shell 442. The cylindrical outer shell 442 serves to protect the internal components and reduce the noise of the motor operation.

[0042] The upper part of the cylindrical outer shell 442 is provided with an end cap 444, which can protect the centrifuge assembly 41 from dust and moisture. The end cap 444 is made of transparent material and works in conjunction with the viewing window of the centrifuge flip cover 42 to facilitate observation of the sample status.

[0043] like Figures 8 to 10 The gene amplification module 5 includes a reaction module 51 and a heat dissipation module 52. The reaction module 51 includes a reaction plate 511 with a temperature control hole array in the middle and a thermoelectric module that provides cooling and heating functions for the reaction plate 511. The temperature control hole array is used to accommodate each reaction tube. An annular pressure plate 512 is tightly attached around the reaction plate 511. Since the gene amplification process will experience frequent temperature changes, the annular pressure plate can prevent the reaction plate 511 from twisting or deforming when it expands and contracts due to thermal expansion and contraction or slight vibration of the instrument, thus maintaining the overall stability of the reaction plate 511.

[0044] The annular pressure plate 512 is equipped with an annular sealing ring 513 to reduce heat loss and make the thermal cycling process of gene amplification more efficient. Specifically, when the reaction plate 511 is heated, the annular sealing ring 513 ensures that heat is mainly concentrated within the reaction area, rather than dissipated into the surrounding environment. Similarly, during the cooling phase, the annular sealing ring 513 also prevents external heat from entering the reaction area, accelerating the cooling rate. This shortens the time of each thermal cycle and improves the overall efficiency of gene amplification.

[0045] The thermoelectric module includes a Peltier device 514 attached to the bottom of the reaction plate 511. Gene amplification requires multiple cycles at different temperatures. Through the effect of the Peltier device 514, better cooling and heating functions are achieved to meet the requirements of precise temperature control. At the same time, the Peltier device 514 has good temperature uniformity, which can make all parts of the reaction plate 511 uniformly heated or cooled, avoiding inconsistent amplification efficiency or inaccurate experimental results caused by uneven temperature.

[0046] The Peltier device 514 has conductors on two opposite sides to improve thermal conductivity. The conductors on both sides can effectively transfer heat from the temperature difference generated by the Peltier effect.

[0047] The heat dissipation module 52 is disposed below the Peltier device 514. The heat dissipation module 52 includes a heat dissipation module 521 with multiple heat dissipation fins and a fan assembly 522 for accelerating heat flow between the heat dissipation fins. To facilitate better heat dissipation of the heat dissipation module 52, air vents 13 are provided on the lower end surface and side surface of the housing 1 corresponding to the heat dissipation module 52.

[0048] A heating cap 515 and a heating circuit board 516 are sequentially arranged above the reaction plate 511, and are fixed at the four corners by screws. The heating cap 515 covers the reaction tube in the temperature control hole to ensure that each reaction tube can be subjected to appropriate pressure to prevent heat loss and liquid evaporation. At the same time, through the heating of the heating circuit board 516, the heating cap 515 can evenly transfer heat to the top of the reaction tube, so that the sample in the reaction tube can be evenly heated in the vertical direction.

[0049] Furthermore, to make the installation process of the hot cover plate 515 more convenient and accurate, a guide component is provided below the hot cover plate 515. In this embodiment, the guide component is an annular groove plate 517, which is disposed on the annular sealing ring 513. The lower end face of the hot cover plate 515 is provided with a continuous circumferential guide surface 5151, which is inserted into the inner wall of the annular groove plate 517.

[0050] Similarly, the gene amplification module 5 also includes an amplification flap 53, which is hinged to the housing 1 and can be locked and opened by an opening / closing button 43. The housing 1 also has a supporting liner 54 that cooperates with the amplification flap 53 to ensure that the amplification flap 53 is in the correct position when closed, and to support the weight of the amplification flap 53. Likewise, the inner wall of the amplification flap 53 has several pin protrusions that cooperate with several pin holes 441 on the supporting liner 54 to prevent the amplification flap 53 from shaking or becoming misaligned after closing.

[0051] Furthermore, the inner wall of the amplification flip cover 53 is also provided with a number of positioning pins 531 for pressing the annular groove plate 517. After the amplification flip cover 53 is closed, the positioning pins 531 are located in the annular groove 5171 of the annular groove plate 517 and abut against the bottom of the annular groove 5171.

[0052] The annular pressure plate 512 and the annular groove plate 517 are screwed to the upper surface of the heat dissipation module 521. Preferably, the upper surface of the heat dissipation module 521 can be insulated by insulating gaskets.

[0053] An operation panel is also provided on the upper surface of the casing 1, and a power plug for external connection is provided on one side of the casing 1.

[0054] In use, the reaction tube containing the liquid sample is placed into the rotor 412. Driven by the centrifugal motor 413, the liquid sample remaining on the wall of the reaction tube is gathered to the bottom of the reaction tube under the action of centrifugal force. The centrifuged and gathered reaction tube is then placed in the gene amplification module 5. Gene amplification is completed under the set temperature and time conditions. After the gene amplification liquid sample is processed accordingly, it is placed in the electrophoresis tank 312 through the electrophoresis tray for electrophoresis.

[0055] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A gene amplification and gene detection integrated machine, characterized in that: The device includes a housing (1), an electrophoresis module (3) disposed on the end face of the housing (1), and a gene amplification module (5) embedded on the end face of the housing (1), as well as a power supply module (2) that provides power to the electrophoresis module (3) and the gene amplification module (5), the power supply module (2) being disposed inside the housing (1); the electrophoresis module (3) includes an electrophoresis shell (311), an electrophoresis tank (312) located inside the electrophoresis shell (311), and electrode rods installed at both ends of the electrophoresis tank (312); the gene amplification module (5) includes a reaction plate (511) with a temperature control hole array in the middle, and a thermoelectric module that provides cooling and heating functions for the reaction plate (511).

2. The gene amplification and gene detection integrated machine according to claim 1, characterized in that: The end face of the housing (1) is also fitted with a centrifugal module (4). The centrifugal module (4) includes a centrifugal assembly (41). The centrifugal assembly (41) includes a centrifugal fixing frame (411) fixed inside the housing (1), a rotor (412) for placing the reaction tube, and a centrifugal motor (413) for driving the rotor (412) and the reaction tube to rotate axially. The centrifugal motor (413) is fixed inside the centrifugal fixing frame (411), and the rotor (412) is connected to the motor shaft of the centrifugal motor (413) through the rotor (412) seat.

3. The gene amplification and gene detection integrated machine according to claim 1, characterized in that: The gene amplification module (5) also includes a heat dissipation module (52) disposed below the reaction plate (511). The heat dissipation module (52) includes a heat dissipation module (521) with multiple heat dissipation fins and a fan assembly (522) for accelerating the heat flow between the heat dissipation fins.

4. The gene amplification and gene detection integrated machine according to claim 1, characterized in that: The gene amplification module (5) also includes an amplification flap (53), which is hinged to the housing (1). The housing (1) is also provided with a support liner (54) that cooperates with the amplification flap (53).

5. The gene amplification and gene detection integrated machine according to claim 1, characterized in that: A heat cover plate (515) and a heating circuit board (516) are arranged sequentially above the reaction plate (511). The heat cover plate (515) and the heating circuit board (516) are fixed to each other by screws. The heat cover plate (515) covers the reaction tube in the temperature control hole.

6. The gene amplification and gene detection integrated machine according to claim 5, characterized in that: The reaction plate (511) is provided with an annular pressure plate (512), which is tightly fitted to the circumferential surface of the reaction plate (511).

7. The gene amplification and gene detection integrated machine according to claim 6, characterized in that: An annular sealing ring (513) is provided between the annular pressure plate (512) and the hot cover pressure plate (515).

8. The gene amplification and gene detection integrated machine according to claim 2, characterized in that: The centrifuge module (4) also includes a centrifuge cover (42), which is hinged to the housing (1). The housing (1) is also provided with a positioning liner (44) that cooperates with the centrifuge cover (42). The inner wall of the centrifuge cover (42) is provided with a number of pin protrusions, which cooperate with a number of pin holes (441) on the positioning liner (44).

9. The gene amplification and gene detection integrated machine according to claim 8, characterized in that: The positioning liner (44) is also provided with a hollow cylindrical shell (442) in the middle. The rotor (412) rotates inside the cylindrical shell (442). The upper part of the cylindrical shell (442) is provided with an end cap (444). The end cap (444) is made of transparent material. The centrifuge flip cover (42) is provided with a viewing window.

10. The gene amplification and gene detection integrated machine according to claim 1, characterized in that: The electrophoresis module (3) also includes a blue light component (32) disposed below the electrophoresis tank (312). The blue light component (32) includes a blue light fixing groove (321) fixed below the end face of the housing (1). A light-emitting plate (322) is provided in the mounting cavity of the blue light fixing groove (321), and the light source is disposed on the light-emitting plate (322). The end face of the housing (1) opposite the light-emitting plate (322) is provided with an opening. Both the electrophoresis shell (311) and the electrophoresis tank (312) are made of transparent material.

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