Nattokinase separation and purification structure

Abstract

The utility model provides a nattokinase separating and purifying structure which comprises a bottom plate, supporting columns are evenly and fixedly connected to one side of the upper end face of the bottom plate, a sealing structure is fixedly connected to the upper end faces of the supporting columns, a cover plate is fixedly connected to the upper end face of the sealing structure, and a centrifugal structure is rotationally connected to the middle of the lower end face of an inner cavity of the sealing structure. A conveying structure is fixedly connected to the middle of the lower end face of an inner cavity of the supporting column, a conveying structure is fixedly connected to the end face of one side of the conveying structure, a separation structure is fixedly connected to the middle of the upper end face of the bottom plate, and a storage structure is fixedly connected to the right side of the separation structure. Therefore, the nattokinase mixed solution is purified during use, the purification efficiency of the nattokinase mixed solution is improved during use, the cost is reduced, and the problems that an existing purification and separation device is complex in structure and poor in separation effect, the activity of nattokinase is affected, the production cost is high and the like are solved. And batch and large-scale production is not convenient.

Description

Technical Field

[0001] This utility model relates to the field of nattokinase isolation and purification technology, and in particular to a nattokinase isolation and purification structure. Background Technology

[0002] Thrombotic diseases, including arterial and venous thrombotic diseases, have become one of the leading causes of death and disability worldwide. Researchers around the world are actively working to overcome this intractable disease, thus creating a huge market potential for thrombolytic drugs. Currently, commonly used antithrombotic drugs are mainly divided into three categories: antiplatelet drugs, anticoagulants, and thrombolytic drugs. While current thrombolytic drugs offer certain therapeutic effects, they also bring corresponding toxic side effects. Nattokinase, as a new generation of thrombolytic drugs, has many advantages, including direct and diversified pharmacological effects, a long in vivo half-life, a relatively small molecular weight allowing direct absorption through the gastrointestinal system, high safety, and low cost. Therefore, the research and development of nattokinase has received further attention and focus.

[0003] The existing equipment has the following problems when in use: the existing purification and separation equipment has a complex structure, poor separation effect, affects the activity of nattokinase, and the high-purity extraction method has high production cost, making it inconvenient for mass production. Utility Model Content

[0004] The problem this invention aims to solve is that existing purification and separation devices have complex structures, poor separation effects, affect the activity of nattokinase, and have high production costs, making them inconvenient for mass production and large-scale manufacturing.

[0005] To solve the above-mentioned technical problems, this utility model provides a nattokinase separation and purification structure including a base plate, a support column uniformly fixedly connected to one side of the upper surface of the base plate, a sealing structure fixedly connected to the upper surface of the support column, a cover plate fixedly connected to the upper surface of the sealing structure, a centrifugal structure rotatably connected to the middle of the lower surface of the inner cavity of the sealing structure, a driving structure fixedly connected to one side of the lower surface of the sealing structure, a transmission structure fixedly connected to the middle of the lower surface of the inner cavity of the support column, a conveying structure fixedly connected to one side of the transmission structure, a separation structure fixedly connected to the middle of the upper surface of the base plate, and a storage structure fixedly connected to the right side of the separation structure.

[0006] The sealing structure includes a working plate fixedly connected to the upper end face of the support column, a sealing frame fixedly connected to the upper end face of the working plate, a waste port fixedly connected to the lower part of the left end face of the sealing frame, a partition plate slidably connected to the inner cavity of the waste port, and a cover plate including a top plate fixedly connected to the upper end face of the sealing frame, and a feed port fixedly connected to the middle part of the upper end face of the top plate.

[0007] Preferably, the centrifugal structure includes a rotating shaft rotatably connected to the middle of the upper end face of the working plate, a centrifugal plate fixedly connected to the upper end face of the rotating shaft, a centrifugal frame fixedly connected to the upper end face of the centrifugal plate, an ultrafiltration plate being snapped into the inner cavity of the centrifugal frame, and a transmission structure being provided in the inner cavity of the rotating shaft.

[0008] Preferably, the drive structure includes a toothed ring rotatably connected to the middle of the lower end face of the work plate, a threaded rod rotatably connected to one side of the lower end face of the work plate, the threaded rod meshing with the toothed ring, a drive motor fixedly connected to the work plate on one side of the threaded rod, the output end of the drive motor being fixedly connected to the threaded rod, and the toothed ring penetrating the work plate and fixedly connected to the rotating shaft.

[0009] Preferably, the transmission structure includes a pressure valve fixedly connected to the lower end face of the inner cavity of the support column, a transmission pipe fixedly connected to the upper end face of the pressure valve, the transmission pipe passing through the toothed ring, the rotating shaft and the centrifugal plate, an electric telescopic rod fixedly connected to the lower end face of the inner cavity of the transmission pipe, and a sealing plug matching the inner cavity of the transmission pipe fixedly connected to the upper end face of the electric telescopic rod.

[0010] Preferably, the conveying structure includes an output pipe fixedly connected to the middle of the right end face of the pressure valve, an output valve fixedly connected to the right end face of the output pipe, a separation structure fixedly connected to the upper end face of the output valve, the separation structure including a connecting plate fixedly connected to the upper end face of the output valve, connecting columns fixedly connected to the four corners of the lower end face of the connecting plate, and a connecting frame fixedly connected to the upper end face of the connecting plate.

[0011] Preferably, the storage structure includes an output port fixedly connected to the lower right end face of the connecting frame, and a storage box fixedly connected to the output port. The storage box is fixedly connected to the middle right side of the upper end face of the base plate.

[0012] Compared with the prior art, this utility model provides a structure for isolating and purifying nattokinase, which has the following beneficial effects:

[0013] 1. This utility model, through the sealing structure on the upper end face of the support column, stores the waste liquid during the purification of nattokinase. The cover plate on the upper end face of the sealing structure facilitates the addition of nattokinase mixture during use. The centrifugal structure within the sealed cavity purifies the nattokinase mixture during use, thereby improving purification efficiency, reducing costs, and solving the problems of existing purification and separation devices that are complex in structure, have poor separation effect, affect nattokinase activity, and have high production costs, making them inconvenient for mass production.

[0014] 2. This utility model utilizes a driving structure on the lower end face of a sealed structure to drive the centrifugal structure during use. A transmission structure within the support column cavity allows for the extraction of purified nattokinase. A conveying structure on one side of the transmission structure transports the purified nattokinase solution. A separation structure in the middle of the upper end face of the base plate separates the purified nattokinase solution during use. A storage structure on one side of the base plate stores the processed nattokinase solution. This allows for further separation of the purified nattokinase solution during use, increasing the purity of the nattokinase mixture and facilitating mass production. Attached Figure Description

[0015] Figure 1 This is a front-view perspective stereoscopic diagram of the present invention;

[0016] Figure 2 This is a rear-view stereoscopic diagram of the present invention;

[0017] Figure 3 This is a half-sectional perspective view of the present invention.

[0018] In the diagram: 1-Base plate, 2-Support column, 3-Sealing structure, 31-Working plate, 32-Sealing frame, 33-Waste port, 34-Partition plate, 4-Cover plate, 41-Top plate, 42-Inlet, 5-Centrifugal structure, 51-Rotating shaft, 52-Centrifugal plate, 53-Centrifugal frame, 54-Ultrafiltration plate, 6-Drive structure, 61-Gear ring, 62-Threaded rod, 63-Drive motor, 7-Transmission structure, 71-Pressure valve, 72-Transmission pipe, 73-Electric telescopic rod, 74-Sealing plug, 8-Conveying structure, 81-Output pipe, 82-Output valve, 9-Separation structure, 91-Connecting column, 92-Connecting plate, 93-Connecting frame, 10-Storage structure, 101-Outlet, 102-Storage box. Detailed Implementation

[0019] This utility model relates to a structure for isolating and purifying nattokinase, such as... Figure 1 As shown, the system includes a base plate 1, with support columns 2 uniformly fixedly connected to one side of the upper surface of the base plate 1, a sealing structure 3 fixedly connected to the upper surface of the support columns 2, a cover plate 4 fixedly connected to the upper surface of the sealing structure 3, a centrifugal structure 5 rotatably connected to the middle of the lower surface of the inner cavity of the sealing structure 3, a driving structure 6 fixedly connected to one side of the lower surface of the sealing structure 3, a transmission structure 7 fixedly connected to the middle of the lower surface of the inner cavity of the support columns 2, a conveying structure 8 fixedly connected to one side of the transmission structure 7, a separation structure 9 fixedly connected to the middle of the upper surface of the base plate 1, and a storage structure 10 fixedly connected to the right side of the separation structure 9.

[0020] Furthermore, the centrifugal structure 5 includes a rotating shaft 51 rotatably connected to the middle of the upper end face of the working plate 31. A centrifugal plate 52 is fixedly connected to the upper end face of the rotating shaft 51, and a centrifugal frame 53 is fixedly connected to the upper end face of the centrifugal plate 52. An ultrafiltration plate 54 is snapped into the inner cavity of the centrifugal frame 53. A transmission structure 7 is provided in the inner cavity of the rotating shaft 51. Through the centrifugal structure 5 in the inner cavity of the sealed structure 3, the nattokinase mixture is purified during use, thereby improving the purification efficiency of the nattokinase mixture and reducing costs.

[0021] Furthermore, the drive structure 6 includes a gear ring 61 rotatably connected to the middle of the lower end face of the working plate 31. A threaded rod 62 is rotatably connected to one side of the lower end face of the working plate 31. The threaded rod 62 meshes with the gear ring 61. A drive motor 63 is fixedly connected to the working plate 31 on one side of the threaded rod 62. The output end of the drive motor 63 is fixedly connected to the threaded rod 62. The gear ring 61 passes through the working plate 31 and is fixedly connected to the rotating shaft 51. Through the drive structure 6 on the lower end face of the sealing structure 3, the centrifugal structure 5 is driven during use.

[0022] Furthermore, the transmission structure 7 includes a pressure valve 71 fixedly connected to the lower end face of the inner cavity of the support column 2. A transmission pipe 72 is fixedly connected to the upper end face of the pressure valve 71. The transmission pipe 72 passes through the toothed ring 61, the rotating shaft 51, and the centrifugal plate 52. An electric telescopic rod 73 is fixedly connected to the lower end face of the inner cavity of the transmission pipe 72. A sealing plug 74 that matches the inner cavity of the transmission pipe 72 is fixedly connected to the upper end face of the electric telescopic rod 73. The purified nattokinase can be extracted during use through the transmission structure 7 in the inner cavity of the support column 2.

[0023] Furthermore, the conveying structure 8 includes an output pipe 81 fixedly connected to the middle of the right end face of the pressure valve 71. An output valve 82 is fixedly connected to the right end face of the output pipe 81. A separation structure 9 is fixedly connected to the upper end face of the output valve 82. The separation structure 9 includes a connecting plate 92 fixedly connected to the upper end face of the output valve 82. Connecting posts 91 are fixedly connected to the four corners of the lower end face of the connecting plate 92. A connecting frame 93 is fixedly connected to the upper end face of the connecting plate 92. The nattokinase purified liquid is conveyed through the conveying structure 8 on one side of the conveying structure 7 during use. The nattokinase purified liquid is separated through the separation structure 9 in the middle of the upper end face of the bottom plate 1 during use.

[0024] Furthermore, the storage structure 10 includes an output port 101 fixedly connected to the lower right end face of the connecting frame 93. The output port 101 is fixedly connected to a storage box 102, which is fixedly connected to the middle right side of the upper end face of the base plate 1. The processed nattokinase solution is stored through the storage structure 10 on one side of the base plate 1, which allows for the re-separation of the nattokinase purified solution during use, thereby increasing the purity of the nattokinase mixture and facilitating mass production.

[0025] Working principle and usage process of this utility model:

[0026] During operation, the nattokinase mixture is poured into the inner cavity of the ultrafiltration plate 54 through the feed port 42. At this time, the drive structure 6 is activated, causing the output end of the drive motor 63 to rotate, which in turn causes the threaded rod 62 to rotate, thus causing the gear 61 to rotate, which in turn causes the rotating shaft 51 to rotate, thus causing the centrifuge plate 52 to rotate, and the centrifuge rack 53 to rotate. This further causes the nattokinase mixture to be filtered and preserved under the action of centrifugal force. At this time, the electric telescopic rod 73 is activated, causing the electric telescopic rod 73 to extend, thus raising the height of the sealing plug 74. The purified nattokinase solution after filtration enters the pressure valve 71 through the transfer pipe 72, and then passes through the pressure valve 71 and the output pipe 81, and then enters the inner cavity of the connecting rack 93 through the output valve 82. At this time, the personnel add (NH4)2SO4 solution and phosphate buffer to the inner cavity of the connecting rack 93 to separate the nattokinase. The separated nattokinase then enters the inner cavity of the storage tank 102 through the output port 101.

[0027] It is understood that this utility model has been described through some embodiments, and those skilled in the art will recognize that various changes or equivalent substitutions can be made to these features and embodiments without departing from the spirit and scope of this utility model. Furthermore, under the teachings of this utility model, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the protection scope of this utility model.

Claims

1. A structure for isolating and purifying nattokinase, comprising a substrate (1), characterized in that, A support column (2) is uniformly fixedly connected to one side of the upper end face of the base plate (1). A sealing structure (3) is fixedly connected to the upper end face of the support column (2). A cover plate (4) is fixedly connected to the upper end face of the sealing structure (3). A centrifugal structure (5) is rotatably connected to the middle of the lower end face of the inner cavity of the sealing structure (3). A driving structure (6) is fixedly connected to one side of the lower end face of the sealing structure (3). A transmission structure (7) is fixedly connected to the middle of the lower end face of the inner cavity of the support column (2). A conveying structure (8) is fixedly connected to one end face of the transmission structure (7). A separation structure (9) is fixedly connected to the middle of the upper end face of the base plate (1). A storage structure (10) is fixedly connected to the right side of the separation structure (9).

2. The nattokinase isolation and purification structure according to claim 1, characterized in that: The sealing structure (3) includes a working plate (31) fixedly connected to the upper end face of the support column (2), a sealing frame (32) fixedly connected to the upper end face of the working plate (31), a waste port (33) fixedly connected to the lower part of the left end face of the sealing frame (32), a partition (34) slidably connected to the inner cavity of the waste port (33), and a cover plate (4) including a top plate (41) fixedly connected to the upper end face of the sealing frame (32), and a feed port (42) fixedly connected to the middle part of the upper end face of the top plate (41).

3. The nattokinase isolation and purification structure according to claim 2, characterized in that: The centrifugal structure (5) includes a rotating shaft (51) rotatably connected to the middle of the upper end face of the working plate (31), a centrifugal plate (52) fixedly connected to the upper end face of the rotating shaft (51), a centrifugal frame (53) fixedly connected to the upper end face of the centrifugal plate (52), an ultrafiltration plate (54) clamped in the inner cavity of the centrifugal frame (53), and a transmission structure (7) provided in the inner cavity of the rotating shaft (51).

4. The nattokinase isolation and purification structure according to claim 3, characterized in that: The drive structure (6) includes a gear ring (61) rotatably connected to the middle of the lower end face of the working plate (31), a threaded rod (62) rotatably connected to one side of the lower end face of the working plate (31), the threaded rod (62) meshing with the gear ring (61), a drive motor (63) fixedly connected to the working plate (31) on one side of the threaded rod (62), the output end of the drive motor (63) fixedly connected to the threaded rod (62), and the gear ring (61) passing through the working plate (31) and fixedly connected to the rotating shaft (51).

5. The nattokinase isolation and purification structure according to claim 4, characterized in that: The transmission structure (7) includes a pressure valve (71) fixedly connected to the lower end face of the inner cavity of the support column (2), a transmission pipe (72) fixedly connected to the upper end face of the pressure valve (71), the transmission pipe (72) passing through the toothed ring (61), the rotating shaft (51) and the centrifugal plate (52), an electric telescopic rod (73) fixedly connected to the lower end face of the inner cavity of the transmission pipe (72), and a sealing plug (74) matching the inner cavity of the transmission pipe (72) fixedly connected to the upper end face of the electric telescopic rod (73).

6. The nattokinase isolation and purification structure according to claim 5, characterized in that: The conveying structure (8) includes an output pipe (81) fixedly connected to the middle of the right end face of the pressure valve (71), an output valve (82) fixedly connected to the right end face of the output pipe (81), a separation structure (9) fixedly connected to the upper end face of the output valve (82), the separation structure (9) includes a connecting plate (92) fixedly connected to the upper end face of the output valve (82), connecting columns (91) fixedly connected to the four corners of the lower end face of the connecting plate (92), and a connecting frame (93) fixedly connected to the upper end face of the connecting plate (92).

7. The nattokinase isolation and purification structure according to claim 5, characterized in that: The storage structure (10) includes an output port (101) fixedly connected to the lower right end face of the connecting frame (93), and a storage box (102) fixedly connected to the output port (101). The storage box (102) is fixedly connected to the middle right side of the upper end face of the base plate (1).

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