Fluorescent light source control circuit for reducing fluorescence quenching

Abstract

The utility model provides a fluorescent light source control circuit capable of reducing fluorescence quenching. The fluorescent light source control circuit comprises a controller, a fluorescent switch assembly, an energy storage assembly, a comparison assembly and a power supply module. When the fluorescent light source is lightened, the energy storage assembly is charged until the voltage value of the energy storage assembly is larger than the reference voltage, the controller controls the fluorescent light source to be turned off, and the reference voltage corresponds to the needed camera exposure time, so that the fluorescent light source is turned off at the same time when the camera exposure is finished, and the irradiation time of the fluorescent light source on the fluorescent section is effectively controlled. The problem of fluorescence slice quenching caused by continuous irradiation of a fluorescence light source is solved.

Description

Technical Field

[0001] This utility model relates to the field of fluorescence imaging technology, and in particular to a fluorescence light source control circuit that reduces fluorescence quenching. Background Technology

[0002] Fluorescent sections face a significant technical challenge in practical applications: fluorescence quenching. Fluorescence quenching refers to the process by which fluorescent substances gradually lose their luminescence ability under continuous illumination, leading to a weakening or even disappearance of the fluorescence signal, severely affecting the imaging quality and resolution of fluorescence microscopy. This phenomenon is mainly caused by continuous irradiation from a fluorescent light source. Photoexcitation not only promotes fluorescence emission but also accelerates the photochemical reactions of fluorescent molecules, leading to changes or degradation in molecular structure, thereby reducing the number of molecules available for luminescence.

[0003] Currently, researchers have adopted various strategies to alleviate fluorescence quenching, including but not limited to: developing novel fluorescent dyes to improve photostability; optimizing microscope imaging parameters, such as reducing excitation light intensity and adjusting exposure time; and employing advanced imaging techniques, such as light-sheet illumination and time-resolved imaging, to reduce the overall illumination time and intensity of the sample. While these methods have extended the duration of fluorescence signals to some extent, they have not fundamentally solved the quenching problem caused by continuous fluorescence illumination. Especially under the requirements of long-term observation or high-resolution imaging, quenching of fluorescent sections remains a pressing technical challenge.

[0004] Therefore, the present invention aims to provide a new device to more effectively control the irradiation time of fluorescent sections by fluorescent light sources, reduce fluorescence quenching, and improve the quality of fluorescence microscopy imaging and the stability of fluorescent markers. Utility Model Content

[0005] To address the aforementioned problems, the purpose of this invention is to provide a fluorescent light source control circuit that reduces fluorescence quenching. During the illumination of the fluorescent light source, the energy storage component is charged until the voltage of the energy storage component exceeds a reference voltage. At this point, the controller turns off the fluorescent light source. The reference voltage corresponds to the required camera exposure time, thus ensuring that the fluorescent light source is turned off simultaneously when the camera exposure ends. This effectively controls the irradiation time of the fluorescent slide by the fluorescent light source, solving the problem of fluorescent slide quenching caused by continuous irradiation by the fluorescent light source.

[0006] This utility model is achieved through the following technical solution:

[0007] A fluorescent light source control circuit for reducing fluorescence quenching includes:

[0008] Controller;

[0009] A fluorescent switch component is connected to the output terminal of the controller and used to control the on or off of the fluorescent light source according to the on or off of the control signal of the controller;

[0010] An energy storage component is connected to the fluorescent switch component, and the energy storage component can store energy when the fluorescent switch component is on.

[0011] A comparison component has input terminals connected to the energy storage component and the controller respectively and an output terminal connected to the controller; the controller inputs a reference voltage to the input terminals of the comparison component;

[0012] An exposure brightness acquisition component is connected to the controller and used to acquire a fluorescent slice exposure brightness standard value and input the fluorescent slice exposure brightness standard value to the controller; the controller is used to output a corresponding reference voltage according to the fluorescent slice exposure brightness standard value;

[0013] A power module is connected to the controller, the fluorescent switch component and the comparison component and supplies power to them.

[0014] Further, the drive circuit further comprises a discharge component connected to the energy storage component and the controller respectively, and the discharge component is used to discharge the stored energy of the energy storage component according to the control of the controller.

[0015] Further, the fluorescent switch component comprises a light emitting diode and a first switch tube; an anode of the light emitting diode is connected to the power module; a cathode of the light emitting diode is connected to one of the electrode terminals of the first switch tube; a control terminal of the first switch tube is connected to the controller; and the other of the electrode terminals of the first switch tube is connected to the energy storage component.

[0016] Further, the energy storage component comprises a first resistor, a second resistor and a first energy storage element; a common terminal of the first resistor and the second resistor is connected to the fluorescent switch component; the other terminal of the first resistor is grounded; the other terminal of the second resistor is connected to the first energy storage element; the other terminal of the first energy storage element is grounded; and the common terminal of the second resistor and the first energy storage element is connected to the input terminal of the comparison component.

[0017] Further, the comparison component comprises a comparator; the input terminals of the comparator are connected to the energy storage component and the controller respectively; the controller inputs a reference voltage to the input terminals of the comparator; and the output terminal of the comparator is connected to the controller.

[0018] Further, the energy storage component further comprises a second capacitor connected in parallel to the first resistor.

[0019] Further, the first energy storage element comprises a first capacitor.

[0020] Further, the discharge assembly comprises a third switch tube, a control end of the third switch tube is connected with the controller, one of electrode ends of the third switch tube is connected with the energy storage assembly, and the other electrode end of the third switch tube is grounded.

[0021] Further, a third resistor is further connected in series between the control end of the first switch tube and the controller.

[0022] Compared with the prior art, the technical scheme and beneficial effects of the utility model are as follows:

[0023] (1) The fluorescent light source control circuit capable of reducing fluorescent quenching of the utility model, through the exposure brightness acquisition assembly and the controller, converts the exposure brightness standard value required by the camera into a reference voltage, charges the energy storage assembly during the lighting period of the fluorescent light source, when the voltage value of the energy storage assembly is greater than the reference voltage, the controller controls the fluorescent light source to be turned off, so that the fluorescent light source is turned off at the same time when the camera exposure is finished, the irradiation time of the fluorescent light source on the fluorescent slice is effectively controlled, the quenching of the fluorescent slice in the scanning process is effectively reduced, and the imaging quality of the fluorescence microscope and the accuracy of the experiment are improved.

[0024] (2) The drive circuit of the utility model further comprises a discharge assembly, when the voltage value of the energy storage assembly is greater than the reference voltage, the fluorescent light source is turned off, and a discharge control signal is sent to the discharge assembly at the same time, the discharge assembly discharges the stored energy of the energy storage assembly according to the discharge control signal, and prepares for the next control of the fluorescent light source irradiation. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 is a principle block diagram of a fluorescent light source control circuit capable of reducing fluorescent quenching provided by the utility model embodiment;

[0026] Figure 2 is a principle diagram of a fluorescent light source control circuit capable of reducing fluorescent quenching provided by the utility model embodiment. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the utility model embodiments clearer, the technical scheme in the utility model embodiments will be described clearly and completely in combination with the drawings in the utility model embodiments. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. It should be understood that the specific embodiments described herein are only used to explain the utility model, and are not used to limit the utility model. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.

[0028] Reference Figure 1The application discloses a fluorescent light source control circuit capable of reducing fluorescent quenching, which comprises a controller, a fluorescent switch assembly, an energy storage assembly, a comparison assembly and a power module. The power module is connected with the controller, the fluorescent switch assembly and the comparison assembly respectively and supplies power for them. The power module can comprise a DC-DC voltage regulating assembly, an AC-DC voltage regulating assembly and the like, and is not limited to specific circuits, and can output power required by each assembly. The power module is a prior art means, and will not be described herein.

[0029] The fluorescent switch assembly is connected with the output end of the controller and is used for controlling the opening or extinguishing of the fluorescent light source according to the on-off of the control signal of the controller. The energy storage assembly is connected with the fluorescent switch assembly, and the energy storage assembly can start energy storage when the fluorescent switch assembly is turned on. The input ends of the comparison assembly are connected with the energy storage assembly and the controller respectively, the energy storage assembly inputs the voltage value thereof to the input end of the comparison assembly, and the controller inputs a reference voltage to the input end of the comparison assembly. The output end is connected with the controller, and the controller is used for outputting a control signal according to the output signal of the comparison assembly to control the on-off of the fluorescent switch assembly. In the embodiment, the required exposure time value is input to the controller, the controller outputs a corresponding reference voltage according to the exposure time value, and the required exposure time is the exposure time of the camera collected in advance by the PC. It is worth noting that the process of obtaining the corresponding reference voltage according to the fluorescent slice exposure time does not involve the application points of the application, and will not be described in detail herein. It is only clear that the reference voltage of the application is related to the camera exposure time, and the irradiation time of the fluorescent light source can be well controlled by comparison with the reference voltage.

[0030] Principle: the controller controls the fluorescent switch assembly to be turned on, so that the fluorescent light source is turned on; meanwhile, the energy storage assembly stores energy, and the energy storage value is compared with the reference voltage input to the two input ends of the comparison assembly, and the comparison result is fed back to the controller. It can be understood that the energy storage value is gradually increased, when the energy storage value is greater than the reference voltage, the output value of the comparison assembly will change, the controller receives the changed output value, controls the fluorescent switch assembly to be disconnected, that is, controls the fluorescent light source to be extinguished. In the embodiment, the reference voltage is related to the camera exposure time, and the camera exposure time can be calibrated before the circuit works, and then input to the controller, and the controller converts the corresponding reference voltage and outputs the reference voltage to the comparison assembly. Therefore, when the required exposure value is reached, that is, the energy storage value of the energy storage assembly is greater than the reference voltage, the controller controls the fluorescent switch assembly to be disconnected, so that the fluorescent light source is extinguished, so as to reduce the irradiation time of the fluorescent light source on the fluorescent slice. Only when the camera needs to be exposed, the fluorescent light source is turned on, and the irradiation time of the fluorescent light source is effectively controlled, so that the influence of the fluorescent light source on the fluorescent slice quenching is greatly reduced, and the stability of the fluorescent signal and the reliability of the experimental result are improved.

[0031] The drive circuit further comprises a discharge component connected to the energy storage component and the controller respectively, when the energy storage value of the energy storage component is greater than the reference voltage, the controller sends a discharge control signal to the discharge component, the discharge component is used for discharging the stored energy of the energy storage component according to the discharge control signal, and preparation is made for the next control of the fluorescent light source irradiation.

[0032] In the embodiment, the fluorescent switch component comprises a light emitting diode D1 and a switch tube Q1, the light emitting diode D1 is the fluorescent light source. The switch tube Q1 is an NMOS tube, the drain of the switch tube Q1 is connected to the cathode of the light emitting diode D1, the anode of the light emitting diode D1 is connected to the power module, the gate of the switch tube Q1 is connected to the controller, the source of the switch tube Q1 is connected to the energy storage component, and the gate of the switch tube Q1 is further connected in series with the resistor R3 between the controller, so as to protect the switch tube Q1. When the controller sends high level to the switch tube Q1, the switch tube Q1 is turned on, and the light emitting diode D1 is lit.

[0033] The energy storage component comprises a resistor R1, a resistor R2 and a first energy storage element, and the first energy storage element comprises a capacitor C1 in the embodiment. The common end of the resistor R1 and the resistor R2 is connected to the source of the switch tube Q1, the other end of the resistor R1 is grounded, the other end of the resistor R2 is connected to the capacitor C1, the other end of the capacitor C1 is grounded, the common end of the resistor R2 and the capacitor C1 is connected to the comparison component, and the capacitor C2 is further connected in parallel across the resistor R1. When the switch tube Q1 is turned on, the power module, the light emitting diode D1, the switch tube Q1 and the resistor R1 to the ground form a loop, the voltage on the resistor R1 charges the capacitor C1 through the resistor R2, and the voltage on the capacitor C1 gradually rises. The voltage value on the capacitor C1 is input to the input end of the comparison component and compared with the reference voltage DAC OUT output by the controller.

[0034] The comparison component comprises a comparator IC1, the voltage value on the capacitor C1 is input to the inverting input end of the comparator IC1, and the reference voltage output by the controller is input to the non-inverting input end of the comparator IC1. When the exposure is insufficient, the switch tube Q1 is continuously turned on, the capacitor C1 is continuously charged, and the voltage value of the capacitor C1 continuously rises. At this time, the comparator IC1 outputs high level to the controller. When the voltage value of the capacitor C1 is greater than the reference voltage, that is, the exposure of the fluorescent slice is sufficient, the comparator IC1 outputs low level to the controller, and the controller receives the low level output by the comparator IC1 and sends low level to the gate of the switch tube Q1, so that the switch tube Q1 is turned off.

[0035] The bleeder assembly comprises a switch tube Q3, the switch tube Q3 adopts NMOS tube, the gate of the switch tube Q3 is connected with the controller, the drain of the switch tube Q3 is connected with the inverting input end of the comparator IC1, and the source of the switch tube Q3 is grounded.When the voltage value of the capacitor C1 is greater than the reference voltage, the controller controls the switch tube Q1 to be cut off, simultaneously controls the switch tube Q3 to be turned on, thereby discharging on the capacitor C1, and preparing for the next fluorescent light source to be turned on.

[0036] The above description shows and describes the preferred embodiments of the utility model, and it should be understood that the utility model is not limited to the form disclosed herein, and should not be regarded as excluding other embodiments, but can be used in various other combinations, modifications and environments, and can be modified within the scope of the utility model concept disclosed herein by the above teaching or related technical or knowledge. And the modification and change made by the person skilled in the art without departing from the spirit and scope of the utility model should be within the protection scope of the utility model claims attached herein.

Claims

1. A fluorescent light source control circuit for reducing fluorescence quenching, characterized in that, include: The controller has an internal exposure time value, and the controller outputs a corresponding reference voltage based on the exposure time value. A fluorescent switch assembly, connected to the output terminal of the controller, is used to control the fluorescent light source to turn on or off according to the control signal of the controller. An energy storage component is connected to the fluorescent switch component. When the fluorescent switch component is turned on, the energy storage component can start storing energy. The comparator has its input terminals connected to the energy storage component and the controller, respectively. The energy storage component inputs its voltage value to the input terminal of the comparator, and the controller inputs a reference voltage to the input terminal of the comparator. The output terminal is connected to the controller, which is used to output a control signal according to the output signal of the comparator to control the opening and closing of the fluorescent switch assembly; The power module is connected to the controller, fluorescent switch assembly, and comparator assembly, and the power module supplies power to them.

2. The fluorescent light source control circuit for reducing fluorescence quenching according to claim 1, characterized in that, It also includes a discharge component connected to the energy storage component and the controller respectively, the discharge component being used to discharge the stored energy of the energy storage component according to the control of the controller.

3. The fluorescent light source control circuit for reducing fluorescence quenching according to claim 1, characterized in that, The fluorescent switch assembly includes a light-emitting diode and a first switching transistor. The anode of the light-emitting diode is connected to the power module, the cathode of the light-emitting diode is connected to one of the terminals of the first switching transistor, the control terminal of the first switching transistor is connected to the controller, and the other terminal of the first switching transistor is connected to the energy storage assembly.

4. The fluorescent light source control circuit for reducing fluorescence quenching according to claim 1, characterized in that, The energy storage component includes a first resistor, a second resistor, and a first energy storage element. The common terminal of the first resistor and the second resistor is connected to the fluorescent switch component. The other end of the first resistor is grounded. The other end of the second resistor is connected to the first energy storage element. The other end of the first energy storage element is grounded. The common terminal of the second resistor and the first energy storage element is connected to the input terminal of the comparator.

5. A fluorescent light source control circuit for reducing fluorescence quenching according to claim 1, characterized in that, The comparison component includes a comparator, the input terminals of which are connected to the energy storage component and the controller, respectively. The controller inputs a reference voltage to the input terminal of the comparator, and the output terminal of the comparator is connected to the controller.

6. A fluorescence light source control circuit for reducing fluorescence quenching according to claim 4, characterized in that, The energy storage component also includes a second capacitor connected in parallel across the first resistor.

7. A fluorescence light source control circuit for reducing fluorescence quenching according to claim 4, characterized in that, The first energy storage device includes a first capacitor.

8. A fluorescence light source control circuit for reducing fluorescence quenching according to claim 2, characterized in that, The discharge assembly includes a third switch tube, the control terminal of which is connected to the controller, one of the terminals of which is connected to the energy storage assembly, and the other terminal of which is grounded.

9. A fluorescent light source control circuit for reducing fluorescence quenching according to claim 3, characterized in that, A third resistor is connected in series between the control terminal of the first switching transistor and the controller.

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