Tissue grinding tube capable of rapidly purifying RNA (Ribonucleic Acid)

Abstract

The utility model provides a tissue grinding tube capable of quickly purifying RNA (Ribonucleic Acid), which relates to the technical field of RNA extraction, and comprises a grinding tube body, an auxiliary grinding piece and a plurality of grinding raised lines, two ends of the grinding tube body are concave inwards to form a grinding cavity, the plurality of grinding raised lines are fixed on the inner wall of the grinding cavity in an annular array, the auxiliary grinding piece is connected with the inner wall of a sealing cover, and the sealing cover is connected with the grinding cavity. When the grinding pipe body is placed in a grinding instrument for use, the auxiliary grinding piece is used for crushing a tissue sample in a grinding cavity during grinding; according to the utility model, during grinding, the movable rod provided with the crushing cutter rotates in an uncertain direction, the crushing cutter can continuously impact a tissue sample with different forces, and by virtue of the high-frequency cutting and crushing actions of the crushing cutter in different directions, the deep disintegration can be carried out on tough fiber tissues, high-toughness meat structures and the like; the internal cell structure and fiber framework are fully destroyed, so that the crushing effect is better realized, and the grinding process is greatly accelerated.

Description

TECHNICAL FIELD

[0001] The utility model relates to RNA extraction technical field especially point to a kind of tissue grinding tube of RNA can be quickly purified. BACKGROUND

[0002] RNA (Ribonucleic Acid) is a molecule widely exists in organism, it plays a vital role in the transmission and expression of genetic information, RNA and DNA (Deoxyribonucleic Acid) together constitute the basis of life. RNA extraction is a very important technology in molecular biology and genomics research, used to isolate RNA from various biological samples. The purpose of RNA extraction is to obtain high-quality, high-purity RNA for subsequent experimental analysis, such as RT-qPCR, Northern blot, RNA sequencing, etc.

[0003] In the process of purifying RNA, tissue grinding tubes play an important role. They are usually designed to ensure that tissue samples can be effectively broken down, so as to release the RNA inside the cells. In the process of grinding tissue samples, the current tissue grinding tube first adds tissue samples and grinding balls into the grinding tube, and then places the grinding tube in the grinder for grinding. The combination mode of the existing grinder and grinding tube often simply relies on the basic shaking and swinging power provided by the grinder to make the grinding balls in the grinding tube roll and collide in a limited space. Due to the lack of auxiliary grinding structure in the grinding tube, it is difficult to effectively guide and synergize the movement track, impact angle and force of the grinding balls. For example, when facing muscle tissue or connective tissue samples with tough texture and complex fiber structure, the grinding balls can only rely on random collision and rolling, and many times they are only repeatedly rubbed on the surface of the sample, which is difficult to truly penetrate into the inside of the tissue and completely tear and crush the tough fiber strands. This results in a long grinding time and a greatly reduced overall grinding efficiency, which not only seriously delays the progress of scientific research experiments, but also consumes a lot of human and material resources, becoming a key shortcoming that needs to be overcome in the current tissue sample grinding work. Therefore, a tissue grinding tube for quickly purifying RNA is proposed. SUMMARY

[0004] To solve the above problems of the prior art, the utility model provides a tissue grinding tube for quickly purifying RNA.

[0005] The technical solution of the utility model is as follows: a tissue grinding tube for quickly purifying RNA, comprising:

[0006] A grinding tube body, both ends of the grinding tube body are concave to form a grinding cavity, and a sealing cover is detachably installed at the end of the grinding cavity;

[0007] a plurality of grinding protrusions fixed to the inner wall of the grinding cavity in a ring array;

[0008] an auxiliary grinding member connected to the inner wall of the sealing cover, which is used to break the tissue sample in the grinding cavity during grinding when the grinding tube body is placed in the grinding instrument.

[0009] the auxiliary grinding member comprises:

[0010] a connecting rod, one end of which is connected to the middle of the inner wall of the sealing cover;

[0011] two rotating rings rotatably arranged on the outside of the connecting rod in an up-down manner, the outer wall of each connecting rod is connected to at least one protrusion, and a movable rod is connected between the two protrusions opposite to each other, and a plurality of layers of breaking knives are mounted on the outside of the movable rod along the length direction.

[0012] Preferably, the breaking knives are single-sided or double-sided.

[0013] Preferably, the grinding protrusions are composed of two arc-shaped protrusions, and a channel for accommodating the passage of the tissue sample is arranged between the arc-shaped protrusions and the inner wall of the grinding cavity.

[0014] Preferably, the inner wall of the sealing cover is provided with an internal thread at the end, and the outer wall of the end of the grinding tube body is provided with an external thread.

[0015] Preferably, a viewing window is embedded on the outside of the sealing cover.

[0016] Compared with the prior art, the utility model has the following beneficial effects:

[0017] 1. When the utility model is used, the movable rod provided with the breaking knives rotates in an indefinite direction during grinding, the breaking knives can continuously impact the tissue sample with different forces, the breaking knives can cut and break the tough fiber tissue, the strong and tough meat structure and the like in different directions and at high frequencies, the internal cell structure and fiber structure are fully destroyed, the crushing effect is better realized, the grinding process is greatly accelerated, and the working efficiency and quality of the overall sample processing are effectively improved.

[0018] 2. When the utility model is used, the grinding protrusions can guide the grinding beads to form more complex and efficient movement tracks in the vibration or stirring process, increase the collision and friction times with the tissue sample, and thus grind the tissue into fine and uniform powder more quickly, improve the grinding efficiency, and be beneficial to the rapid purification of RNA. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description, obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor.

[0020] Figure 1 It is an explosive structure schematic view of the present application;

[0021] Figure 2 It is an appearance structure schematic view of the present application;

[0022] Figure 3 It is a structure schematic view of the grinding tube body of the present application;

[0023] Figure 4 It is a cross-sectional structure schematic view of the grinding tube body of the present application;

[0024] Figure 5 It is a structure schematic view of the auxiliary grinding part of the present application.

[0025] In the figure: 1, grinding tube body; 2, sealing cover; 3, grinding cavity; 4, auxiliary grinding part; 41, connecting rod; 42, rotating ring; 43, convex strip; 44, movable rod; 45, crushing knife; 5, grinding convex strip; 6, visible window; 7, external thread; 8, internal thread. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present application will be described clearly and completely below in combination with the drawings in the embodiments of the present application, obviously, the described embodiments are only some embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative labor are within the protection scope of the present application.

[0027] The present application provides a kind of tissue grinding tube of RNA that can be quickly purified as Figures 1-5 As shown in the figure, the present application provides a kind of tissue grinding tube of RNA that can be quickly purified, including grinding tube body 1, the two ends of grinding tube body 1 are concave and form grinding cavity 3, grinding cavity 3 is used to hold tissue sample and grinding ball, the end of grinding cavity 3 is detachably installed with sealing cover 2, the detachable sealing cover 2 is convenient to disassemble, and visible window 6 is embedded on the outside of sealing cover 2, visible window 6 is made of transparent material, by means of visible window 6, staff can know the grinding condition of tissue sample in the grinding tube without opening sealing cover 2.

[0028] In addition, the tissue grinding tube capable of quickly purifying RNA further comprises grinding protrusions 5 and an auxiliary grinding part 4, the grinding protrusions 5 are arranged in a ring array on the inner wall of the grinding cavity 3, and the auxiliary grinding part 4 is connected to the inner wall of the sealing cover 2.

[0029] As shown in Figure 4 Preferably, the grinding protrusions 5 are composed of two arc-shaped protrusions, and a channel for passing the tissue sample is arranged between the arc-shaped protrusions and the inner wall of the grinding cavity 3.

[0030] When the grinding beads move in the grinding cavity 3, the grinding protrusions 5 can guide the grinding beads to form more complex and efficient movement trajectories during vibration or stirring, increase the collision and friction times with the tissue sample, and thus grind the tissue into fine and uniform powder more quickly, improve the grinding efficiency, and facilitate the subsequent rapid purification of RNA.

[0031] In addition, the grinding protrusions 5 can also be replaced by spiral or wavy protrusion tracks arranged in the tube to guide the grinding beads to form more complex and efficient movement trajectories during vibration or stirring, and increase the collision and friction times with the tissue sample.

[0032] As shown in Figure 5 The auxiliary grinding part 4 comprises:

[0033] The connecting rod 41 is arranged along the length direction of the grinding tube body 1, and one end of the connecting rod 41 is connected to the middle of the inner wall of the sealing cover 2.

[0034] The two rotating rings 42 are arranged above and below, and the two rotating rings 42 are rotatably arranged outside the connecting rod 41, preferably mounted by bearings, and at least one protrusion 43 is connected to the outer wall of each connecting rod 41, and the movable rod 44 is connected between the two protrusions 43 opposite to each other, the end of the movable rod 44 is connected to the protrusion 43 through a bearing, and a plurality of crushing knives 45 are mounted on the outer part of the movable rod 44 along the length direction, and the crushing knives 45 are single-edged or double-edged.

[0035] In summary, the two grinding cavities 3 have the powerful ability to grind two types of tissue samples simultaneously, greatly improving the grinding efficiency. In specific use, the tissue samples to be ground can be added to the grinding cavity 3, and the grinding balls are then added. Then, the sealing cover 2 is covered, and the grinding tube is placed in the grinder. After that, the grinder is started to perform the grinding operation. During grinding, the grinding balls move inside the grinding cavity 3 to grind the tissue samples. At the same time, the grinding balls collide with the grinding protrusions 5, causing them to instantaneously change direction and speed, and the movement trajectory becomes more complex and variable. This sudden change makes the subsequent impact angle of the grinding balls on the tissue samples more elusive and the force distribution more diverse, allowing the tissue samples to be ground into fine powder more quickly and completely, greatly improving the grinding precision, uniformity, and overall effect, and laying a solid foundation for subsequent experimental steps.

[0036] In addition, during grinding, the rotating ring 42 can rotate by collision, and the movable rod 44 with the crushing knife 45 installed can also rotate in an indefinite direction. When the movable rod 44 is in an indefinite rotating state, the crushing knife 45 can continuously impact the tissue samples with different forces. Especially for tissue samples with strong toughness, conventional grinding methods often take a long time and do not completely crush the samples. However, at this moment, the multi-directional and high-frequency cutting and crushing action of the crushing knife 45 can deeply disintegrate the tough fiber tissue and strong meat structure, fully destroy the internal cell structure and fiber structure, and better achieve the crushing effect, accelerate the release of RNA, and greatly speed up the grinding process, effectively improving the work efficiency and quality of the overall sample processing.

[0037] If the crushing knife 45 is single-edged, the edges of adjacent two layers of crushing knives 45 can be oppositely arranged, so that the edges can cut the tissue samples during forward and reverse rotation, thereby accelerating the overall grinding process and significantly improving work efficiency and quality.

[0038] For the detachable connection of the sealing cover 2, the inner wall end of the sealing cover 2 is provided with an internal thread 8, and the outer wall end of the grinding tube body 1 is provided with an external thread 7. The sealing cover 2 is connected to the end of the grinding tube body 1 by the internal thread 8. A sealing ring can be provided at the connection to improve the sealing performance after connection.

[0039] When installing the sealing cover 2, the sealing cover 2 can be directly screwed onto the end of the grinding tube body 1 provided with the external thread 7, thereby achieving efficient sealing and stable assembly, providing a solid structural support and connection guarantee.

[0040] The above merely describes preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A tissue grinding tube for rapid purification of RNA, characterized in that, The application relates to a grinding tube body (1) which is internally concave at both ends to form a grinding cavity (3), the end of the grinding cavity (3) is detachably provided with a sealing cover (2), a plurality of grinding protrusions (5) are fixed in an annular array on the inner wall of the grinding cavity (3), and an auxiliary grinding part (4) is connected to the inner wall of the sealing cover (2) and is used for crushing a tissue sample in the grinding cavity (3) during grinding when the grinding tube body (1) is placed in a grinder. The auxiliary grinding part (4) comprises a connecting rod (41) which is connected to the middle of the inner wall of the sealing cover (2), two rotating rings (42) which are arranged on the outer part of the connecting rod (41) in an up-down and rotatable mode, at least one protrusion (43) is connected to the outer wall of each connecting rod (41), a movable rod (44) is connected between the two protrusions (43) which are opposite to each other in an up-down mode, and a plurality of crushing knives (45) are arranged on the outer part of the movable rod (44) in a length direction. The crushing knives (45) are single-edged or double-edged. The grinding protrusions (5) are composed of two arc-shaped protrusions, and a channel for passing the tissue sample is arranged between the arc-shaped protrusions and the inner wall of the grinding cavity (3).

2. The RNA-quickly-purifiable tissue grinding tube according to claim 1, wherein: An inner thread (8) is arranged at the end of the inner wall of the sealing cover (2), and an outer thread (7) is arranged at the outer wall of the end of the grinding tube body (1). A visual window (6) is embedded on the outer part of the sealing cover (2). ​ 3. The RNA-quickly-purifiable tissue grinding tube according to claim 2, wherein: ​ 4. The RNA-quickly-purifiable tissue grinding tube according to claim 1, wherein: ​ 5. The RNA-quickly-purifiable tissue grinding tube according to claim 1, wherein: ​ 6. The RNA-quickly-purifiable tissue grinding tube according to claim 1, wherein: ​

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