Rapid gel preparation device applied to convective immunoelectrophoresis gel
By designing a rapid gel preparation device that includes an adjustment block and a hole needle, the problem of existing devices being unable to make holes was solved, enabling rapid gel preparation and uniform hole making, thus improving the accuracy and repeatability of electrophoresis experiments.
Patent Information
- Application Number
- CN202422633881.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-30
- Publication Date
- 2025-11-07
- Estimated Expiration
- 2034-10-30
AI Technical Summary
Existing rapid gel preparation devices for electrophoresis experiments cannot effectively meet the gel perforation requirements in counterimmunoelectrophoresis, resulting in limitations on the addition of antigens and antibodies and the formation of precipitation lines during the experiment.
A rapid gel dispensing device was designed, comprising an outer shell, an adjusting block, an adjusting gear, a return spring, and a pin. By sliding the adjusting block, the elastic potential energy of the return spring is increased, which drives the adjusting gear to rotate, thereby realizing the up and down movement of the pin to complete the punching and even distribution of the gel.
It enables rapid gel preparation and uniform perforation, ensuring the accuracy and repeatability of electrophoresis experiments and meeting the experimental requirements of counterimmunoelectrophoresis.
Smart Images
Figure CN223519820U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model provides a kind of fast glue device, belong to the technical field of immunoelectrophoresis gel, especially involve a kind of fast glue device applied to immunoelectrophoresis gel. BACKGROUND
[0002] Electrophoresis experiment is a separation technique that utilizes electric field to drive charged particles to migrate in a medium. In the fields of biology, chemistry and medicine, electrophoresis experiment is commonly used to separate, identify and analyze proteins, nucleic acids, polysaccharides and other charged biological macromolecules. Electrophoresis experiment is based on the different migration speeds of different molecules in an electric field, thereby realizing the separation of each component in the sample. Immunoelectrophoresis is an immunological method that combines electrophoresis technology and immunodiffusion technology. Its core principle is to detect and quantify specific proteins by using the relative migration of antigens and antibodies in agar gel under the action of an electric field. During electrophoresis, the two components migrate towards each other, meet between the two wells, and form a visible precipitate line at the appropriate proportion.
[0003] The existing fast glue device for electrophoresis experiment mainly aims to assist the addition of glue, however, these devices have limitations in adapting to the gel configuration required for immunoelectrophoresis. Specifically, they cannot effectively meet the auxiliary requirements of gel punching in immunoelectrophoresis. Immunoelectrophoresis requires gel punching during the configuration process to facilitate the addition of antigens and antibodies and subsequent electrophoresis separation and precipitate line formation. SUMMARY
[0004] In order to make up for the shortcomings of the prior art, the present application provides a fast glue device for immunoelectrophoresis gel, which solves the problem that the existing gel configuration device for electrophoresis experiment cannot meet the requirements of immunoelectrophoresis.
[0005] In order to solve the above technical problems, the utility model provides the following technical scheme: a kind of fast glue device applied to immunoelectrophoresis gel, including shell body, the inside of shell body is equipped with and is corresponding with the adjusting block of itself, adjusting block is connected with the adjusting plate of being placed in one side of itself in the inside by adjusting gear, the lower fixed connection of adjusting plate has hole needle, the inside of shell body is equipped with and is fixedly connected with the reset tension spring of adjusting block, the both sides of adjusting gear are fixedly connected with reset torsion spring.
[0006] Preferably, the shell body is provided with a glue comb placed on both sides of the adjusting block and penetrating through one side of the shell body, the glue comb is fixedly connected with a connecting plate corresponding to the shell body, the connecting plate is fixedly connected with a plurality of evenly distributed protrusions on the side close to the adjusting gear, and the protrusions are engaged with the adjusting gear.
[0007] Preferably, a cover is movably connected to the side of the connecting plate away from the adjusting gear, and the connecting plate is provided with a feeding opening penetrating through the connecting plate and corresponding to the comb.
[0008] Preferably, the adjusting plate is connected with connecting rods arranged on both sides of the adjusting gear on the side close to the adjusting block, both ends of the connecting rods are provided with fixing shafts penetrating through the connecting rods, the fixing shaft close to the adjusting gear is fixedly connected with the gear teeth of the adjusting gear, and the fixing shafts are arranged in the adjusting plate.
[0009] Preferably, the adjusting block is provided with a rotating groove corresponding to the adjusting rod, and the outer side of the rotating groove is connected with a limiting piece fixedly connected with the adjusting plate through a T-shaped limiting groove.
[0010] The one or more technical solutions provided in the embodiments have at least the following technical effects or advantages:
[0011] The adjusting block arranged in the shell body is set, the elastic potential energy of the reset spring is increased by sliding of the adjusting block, when the elastic potential energy is sufficient to twist the reset torsion spring, the adjusting gear is rotated, thereby driving the adjusting plate on one side to slide closely to the adjusting block, and then driving the hole needle to move up and down, so that the punching is realized.
[0012] Other advantages, objects and features of the present application will be clarified to some extent in the following description, and to some extent, it will be obvious to those skilled in the art based on the following description, or can be taught from the practice of the present application. BRIEF DESCRIPTION OF DRAWINGS
[0013] Figure 1 It is a connecting sectional view of the present application applied to the rapid gel preparation device for counter immunoelectrophoresis gel;
[0014] Figure 2 It is an exploded view of the adjusting block of the present application applied to the rapid gel preparation device for counter immunoelectrophoresis gel;
[0015] Figure 3 It is a sectional view of the adjusting block of the present application applied to the rapid gel preparation device for counter immunoelectrophoresis gel.
[0016] Figure 4 It is a sectional view of the shell body of the present application applied to the rapid gel preparation device for counter immunoelectrophoresis gel.
[0017] As shown in the figure:
[0018] 1, shell body;
[0019] 11, reset spring; 12, glue comb; 13, protrusion; 14, inlet;
[0020] 2, adjusting block;
[0021] 21, adjusting gear; 22, adjusting plate; 23, needle; 24, reset torsion spring; 25, connecting rod; 26, fixed shaft; 27, rotating groove; 28, limiting groove; 29, limiting piece. DETAILED DESCRIPTION
[0022] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.
[0023] It should be noted that the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only embodiments.
[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs; the terms used in the specification of the present application are only for the purpose of describing specific embodiments and are not intended to limit the present application; the term "and / or" used herein includes any and all combinations of one or more related listed items.
[0025] As shown in Figure 1 and Figure 2 A rapid gel preparation device for counter immunoelectrophoresis gel, which comprises an outer shell 1, characterized in that: the inside of the outer shell 1 is provided with a corresponding adjusting block 2, the inside of the adjusting block 2 is connected with a adjusting plate 22 placed on one side of itself through an adjusting gear 21, a needle 23 is fixedly connected below the adjusting plate 22, the inside of the outer shell 1 is provided with a reset spring 11 fixedly connected with the adjusting block 2, reset torsion springs 24 are fixedly connected on both sides of the adjusting gear 21, glue combs 12 are provided in the outer shell 1 and penetrate through one side of the plate body on both sides of the adjusting block 2, connecting plates corresponding to the outer shell 1 are fixedly connected above the glue combs 12, a plurality of protrusions 13 evenly distributed are fixedly connected on one side of the connecting plate close to the adjusting gear 21, and the protrusions 13 are engaged with the adjusting gear 21 correspondingly.
[0026] The utility model discloses a kind of innovative gel punching mechanisms, its core is in one inside the adjusting block 2 of shell body 1.By the translational motion of adjusting block 2, the elastic potential energy stored by reset spring 11 can be effectively increased.When this potential energy accumulates to a certain extent, enough to overcome and twist reset torsion spring 24, it will cause the rotational motion of adjusting gear 21.This rotating action further promotes the sliding of adjusting plate 22 on one side closely following adjusting block 2 by mechanical transmission system.In this process, the movement of adjusting plate 22 directly drives orifice needle 23 to realize up and down movement, to complete accurate punching operation.
[0027] The utility model not only can realize the rapid preparation of gel, but also can accurately punch in the equal division process of gel, which is crucial for experiments such as flow immunoelectrophoresis that require accurate punching. Through this design, the performance of the gel in electrophoresis experiments can be ensured, meeting the strict requirements of experiments on gel porosity and uniformity, thereby improving the accuracy and repeatability of experiments.
[0028] As shown in Figure 3 And Figure 4 The connecting plate is movably connected with a cover on the side away from the adjusting gear 21, and the connecting plate is provided with a pouring port 14 penetrating through itself and corresponding to the gel comb 2. The adjusting plate 22 is connected with connecting rods 25 placed on both sides of the adjusting gear 21 on the side close to the adjusting block 2. Both ends of the connecting rod 25 are provided with fixed shafts 26 penetrating through themselves. The fixed shaft 26 close to the adjusting gear 21 is fixedly connected with the gear teeth of the adjusting gear 21, and the other fixed shaft 26 is placed inside the adjusting plate 22. The adjusting block 2 is provided with a rotating groove 27 corresponding to the adjusting rod 25. The outer side of the rotating groove 27 is connected with a limiting piece 29 fixedly connected with the adjusting plate 22 through a T-shaped limiting groove 28.
[0029] In this embodiment, the orifice needle 23 is placed between the adjusting block 2 and the gel comb 12. The gel is discharged to the groove position on both sides by means of the arc-shaped protrusion at the front end of the adjusting block 2. When the adjusting gear 21 rotates away from the protrusion 13, the adjusting gear 21 drives the corresponding fixed shaft 26 to rotate upward, thereby moving one end of the connecting rod 25. The other end of the connecting rod 25 drives the adjusting plate 22 to move. The adjusting plate 22 closely abuts against the adjusting block 2 to slide up and down by means of the limiting piece 29, thereby realizing the up and down movement of the orifice needle and punching the gel on both sides of the adjusting block 2. With repeated movement of the adjusting block 2, the orifice needle 23 repeatedly moves to punch holes, ensuring that the gel in the gel comb 12 has holes. The pouring port 14 is used to add protein samples between the gel comb 12 after the gel is configured.
[0030] In use:
[0031] 1. Preparation stage:
[0032] Make sure all parts are properly installed
[0033] Check the inlet 14 on the connecting plate 1 is clear, make sure the cover is properly installed.
[0034] 2、Gel preparation:
[0035] Gel solution is added to the connecting plate above the gel comb 12 through the inlet 14. The gel solution will be evenly distributed to both sides of the gel comb 12 through the connecting plate.
[0036] Use the arc-shaped protrusion at the front end of the adjustment block 2 to direct the gel solution to the groove position on both sides of the gel comb 12, forming a gel strip.
[0037] 3、Punching operation:
[0038] By operating the device, the adjustment block 2 performs a translational motion, thereby increasing the elastic potential energy stored by the return spring 11.
[0039] When the elastic potential energy reaches a certain value, the return torsion spring 24 is twisted, triggering the rotation of the adjustment gear 21.
[0040] The rotation of the adjustment gear 21 moves the adjustment plate 22 through the fixed shaft 26 and connecting rod 25, and the movement of the adjustment plate 22 moves the hole needle 23 up and down, achieving precise punching.
[0041] The repeated movement of the adjustment block 2 causes the hole needle 23 to repeatedly punch the gel strip, ensuring that the gel strip forms evenly distributed holes.
[0042] 4、Sample addition:
[0043] After the gel is punched, protein samples or other reagents required for experiments are added to the gel strip between the gel combs 12 through the inlet 14.
[0044] 5、Electrophoresis preparation:
[0045] The gel combs 12 containing the gel and sample are removed from the outer housing 1 and placed into the electrophoresis tank, preparing for the convection immunoelectrophoresis experiment.
[0046] 6、Electrophoresis experiment:
[0047] According to the experimental steps of convection immunoelectrophoresis, perform electrophoresis operation, observe and record the experimental results.
[0048] 7、Cleaning and maintenance:
[0049] After the experiment is completed, disassemble the device and clean each part, especially the hole needle 23 on the gel comb 12 and the adjustment plate 22, to ensure the next use of the device.
[0050] Although the utility model has disclosed as above with preferable embodiments, it is not used to limit the utility model, anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the utility model, therefore the protection scope of the utility model should be limited by the claims.
Claims
1. A rapid gel preparation device for application to a counter immunoelectrophoresis gel comprising an outer housing (1), characterised in that: The inside of the outer shell (1) is provided with a corresponding adjusting block (2), the inside of the adjusting block (2) is connected with an adjusting plate (22) on one side through an adjusting gear (21), the lower part of the adjusting plate (22) is fixedly connected with a hole needle (23), the inside of the outer shell (1) is provided with a reset tension spring (11) fixedly connected with the adjusting block (2), and the two sides of the adjusting gear (21) are fixedly connected with reset torsion springs (24).
2. The rapid gel preparation device for the counter immunoelectrophoresis gel according to claim 1, characterized in that: The inside of the outer shell (1) is provided with a rubber comb (12) disposed on both sides of the adjusting block (2) and penetrating through one side of the plate body, the upper part of the rubber comb (12) is fixedly connected with a corresponding connecting plate of the outer shell (1), one side of the connecting plate close to the adjusting gear (21) is fixedly connected with a plurality of evenly distributed protrusions (13), and the protrusions (13) are correspondingly engaged with the adjusting gear (21).
3. A rapid gel casting device for a counter immunoelectrophoresis gel according to claim 2, characterized in that: The side of the connecting plate away from the adjusting gear (21) is movably connected with a cover, and the connecting plate is provided with a throwing port (14) penetrating through itself and corresponding to the rubber comb (12).
4. The device for rapid preparation of gel for counter immunoelectrophoresis according to claim 1, wherein: The side of the adjusting plate (22) close to the adjusting block (2) is connected with a connecting rod (25) disposed on both sides of the adjusting gear (21), both ends of the connecting rod (25) are provided with fixed shafts (26) penetrating through themselves, the fixed shaft (26) close to the adjusting gear (21) is fixedly connected with the gear teeth of the adjusting gear (21), and the other fixed shaft (26) is disposed in the adjusting plate (22).
5. A rapid gel casting device for a counter immunoelectrophoresis gel as claimed in claim 4, wherein: The inside of the adjusting block (2) is provided with a rotating groove (27) corresponding to the connecting rod (25), and the outer side of the rotating groove (27) is connected with a limiting piece (29) fixedly connected with the adjusting plate (22) through a T-shaped limiting groove (28).