Integrated nano-liter injection pump
By using an airbag to compress and fix the glass capillary, the problem of easy breakage of glass capillary in nanoliter microinjection pumps is solved, achieving more efficient experimental operation and lower material costs.
Patent Information
- Application Number
- CN202422485490.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-15
- Publication Date
- 2025-11-07
- Estimated Expiration
- 2034-10-15
AI Technical Summary
Existing nanoliter microinjection pumps are prone to breakage when the glass capillary is fixed, which affects injection accuracy and makes cleaning difficult, thus affecting experimental results.
The glass capillary is fixed by airbag compression, which uses uniform air pressure to avoid hard contact and local stress concentration, reducing the risk of breakage. The use of annular airbags and limiting channels ensures accurate positioning.
It effectively reduces the risk of glass capillary tube breakage during needle loading, simplifies the cleaning process, improves experimental efficiency and accuracy, and reduces experimental material costs.
Smart Images
Figure CN223522492U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the field of biomedical technology, and particularly relates to an integrated nanoliter injection pump. BACKGROUND
[0002] In biomedical research, nanoliter microinjection technology is an important means for intracellular injection, gene injection, drug delivery and other experiments. It is mainly used for quantitative and constant-speed nanoliter microinjection or suction operation of egg cells, fish embryos, insect larvae, protozoa, animal brain tissues and the like.
[0003] At present, nanoliter microinjection pumps are widely used in the microinjection process of cells or tissues. They mainly realize the microinjection of cells or tissues through a micro pump, an injection needle and a glass capillary tube. The main working principle is that the injection needle is driven by a pushing mechanism inside the pump body to slowly advance from one end of the glass capillary tube, and then the liquid in the tube is accurately pushed to the target position, so that accurate microinjection is realized. However, the interface of the existing nanoliter microinjection pump for connecting the glass capillary tube usually adopts a split structure, and a threaded compression fixing method is used when the needle is installed. The glass capillary tube is a disposable consumable, and its tube diameter is extremely small. This fixing method requires a high compression force during operation, which easily causes the glass capillary tube to break at the fixed position due to pressure loss, and then generates debris. These debris not only affect the injection accuracy, but also are very difficult to clean up, which easily interferes with the experimental results. Therefore, the utility model provides an integrated nanoliter injection pump to reduce the damage of the glass capillary tube, simplify the cleaning process, and improve the experimental efficiency and accuracy.
[0004] However, an effective solution has not been proposed for the problems in the related art. TECHNICAL SOLUTION
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical scheme: an integrated nanoliter injection pump, comprising a pump body, an interface is arranged at the front end of the pump body, a needle pushing body penetrates through the interface, a needle fixing assembly is arranged at the interface, the needle fixing assembly comprises a connector, a sealing gasket and a gasket, the connector is sleeved and fixed outside the interface, a fixing cavity is formed in the inside of the connector, an installation cavity is connected to one end of the fixing cavity, the sealing gasket is installed in the inside of the installation cavity, the gasket is abutted at the interface of the pump body through the sealing gasket, an annular air bag is fixed to the inner wall of the fixing cavity, air exchange channels are formed at both sides of the connector, one of the air exchange channels is provided with a control air valve at the end, the other air exchange channel is connected with an air pipe, an inflation air bag is installed at one end of the air pipe, a one-way air valve is installed on the air pipe body, and a limiting channel is formed in the center of the connector, the sealing gasket and the gasket.
[0006] As a preferred technical scheme of the utility model, the limiting channel at the center of the joint, the sealing gasket and the gasket and the center opening of the annular air bag are arranged in a line, and the push pin body of the pump body respectively penetrates the limiting channel at the center of the joint, the sealing gasket, the gasket and the center opening of the annular air bag.
[0007] As a preferred technical scheme of the utility model, the limiting channel comprises a capillary channel and a push pin channel, and the capillary channel and the push pin channel are communicated.
[0008] As a preferred technical scheme of the utility model, one end of the capillary channel is connected with the push pin channel, and the other end of the capillary channel is provided with a guide opening.
[0009] As a preferred technical scheme of the utility model, the inflatable air bag, the air pipe and the one-way air valve are fixed to the outer wall of the joint.
[0010] Compared with the prior art, the utility model has the beneficial effects that: the utility model adopts the mode that the air bag extrudes the glass capillary tube to fix the glass capillary tube through uniform air pressure, avoids hard contact and local stress concentration, thereby effectively reduces the risk that the glass capillary tube is broken due to uneven stress in the process of needle installation, reduces the difficulty and time of cleaning, avoids the interference of experimental results, improves experimental efficiency, reduces experimental material cost, is simple and easy to operate, and is easy to master. BRIEF DESCRIPTION OF DRAWINGS
[0011] The drawings are used to provide further understanding of the utility model, and constitute a part of the specification, are used together with the embodiments of the utility model to explain the utility model, and do not constitute the limitation to the utility model.
[0012] Figure 1 It is the whole structure schematic diagram of the utility model;
[0013] Figure 2 It is the sectional structure schematic diagram of the joint in the utility model;
[0014] Figure 3 It is the structure schematic diagram of the joint inserted into the glass capillary tube in the utility model;
[0015] Figure 4 It is the structure schematic diagram of the joint in the utility model;
[0016] In the drawing: 1, pump body; 2, interface; 3, joint; 4, sealing gasket; 5, gasket; 6, fixed cavity; 7, installation cavity; 8, annular air bag; 9, air exchange channel; 10, control air valve; 11, air pipe; 12, inflatable air bag; 13, one-way air valve; 14, capillary channel; 15, push pin channel; 16, guide opening; 17, push pin body. DETAILED DESCRIPTION
[0017] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model.
[0018] Embodiment
[0019] Please refer to Figures 1-4 The utility model provides the following technical scheme: a kind of integrated nanoliter grade injection pump, including pump body 1, this is the basic part of entire device, for realizing the recycling of push needle body 17 is pushed out;Pump body 1 front end is provided with interface 2, interface 2 is provided with fixed needle assembly, the assembly is used to fix glass capillary and ensure the sealing property in microinjection process.Fixed needle assembly includes connector 3, sealing washer 4 and gasket 5, sealing washer 4 and gasket 5 are used to ensure the sealing property in injection process;Connector 3 is sleeved and fixed in the outside of interface 2, and fixed cavity 6 is opened in the inside of connector 3, one end of fixed cavity 6 is connected with installation cavity 7, sealing washer 4 is installed in the inside of installation cavity 7, gasket 5 is butted at the interface 2 of pump body 1 by sealing washer 4, and the inner wall of fixed cavity 6 is fixed with annular air bag 8, for fixing glass capillary when being inflated;Connector 3 is provided with air passage 9 on the both sides of annular air bag 8, for adjusting the air pressure in annular air bag 8;Two air passages 9 are all communicated with the inside of annular air bag 8, and one end of one air passage 9 is installed with control air valve 10, control air valve 10 is used to control the discharge of gas, so as to remove glass capillary after use;The other end of the other air passage 9 is connected with air pipe 11, one end of air pipe 11 is installed with inflation air bag 12, for manually inflating annular air bag 8;Air pipe 11 pipe body is installed with one-way air valve 13, one-way air valve 13 ensures that gas can only flow in one direction, to prevent the backflow of gas in annular air bag 8;The center of connector 3, sealing washer 4 and gasket 5 is all provided with limiting channel, for providing positioning during installation or disassembly of capillary, to ensure that each component is correctly installed in place.
[0020] In order to ensure the penetration of capillary, in the embodiment, as a preferred technical scheme of the utility model, the limiting channels in the center of connector 3, sealing washer 4 and gasket 5 and the center port of annular air bag 8 are arranged in line, and the push needle body 17 of pump body 1 respectively penetrates the limiting channels in the center of connector 3, sealing washer 4 and gasket 5 and the center port of annular air bag 8.
[0021] In order to ensure the fit of capillary and push needle body 17, in the embodiment, as a preferred technical scheme of the utility model, the limiting channel includes capillary channel 14 and push needle channel 15, and the capillary channel 14 and the push needle channel 15 are communicated.
[0022] In order to facilitate the guiding of the capillary tube to insert into the limiting channel, in the embodiment, as a preferred technical scheme of the utility model, one end of the capillary tube channel 14 is connected with the push pin channel 15, and the other end of the capillary tube channel 14 is provided with a guiding port 16.
[0023] In order to facilitate the operation and increase the overall aesthetic property, in the embodiment, as a preferred technical scheme of the utility model, the inflatable air bag 12, the air pipe 11 and the one-way air valve 13 are fixed to the outer wall of the joint 3.
[0024] In summary, by means of the above technical scheme of the utility model, when in use, firstly, it is ensured that the glass capillary tube has been properly treated according to the experimental requirements, such as being drawn into a proper needle tip size. At the same time, it is checked whether the pump body 1 and the rest of the accessories are clean and undamaged, and the necessary tools, such as a clamp, are prepared. The injection material is sucked into the tip of the capillary tube by means of the capillary phenomenon, and then the treated glass capillary tube is carefully inserted into the joint 3 at the interface 2 of the pump body 1, and the guiding port 16 is inserted into the capillary tube channel 14, and at the same time, the push pin body 17 of the pump body 1 penetrates the glass capillary tube and is inserted into the inside of the capillary tube, until the capillary tube port end abuts at the connection between the capillary tube channel 14 and the push pin channel 15, then the thumb presses the inflatable air bag 12, and air enters the inside of the annular air bag 8 from the air pipe 11 to make the annular air bag 8 bump and expand, and then abut against the outer wall of the capillary tube, so as to ensure that the capillary tube can be stably fixed; then the pump body 1 is fixed on the test fine adjustment support, and according to the distance, angle and injection depth between the capillary tube and the sample and other parameters, the position of the tip of the capillary tube is accurately adjusted, and finally the injection operation is performed on the push pin body 17 by means of the advancing mechanism inside the pump body 1.
[0025] Finally, it should be pointed out that: in the utility model, unless otherwise explicitly specified and limited, the terms "mounting", "setting", "connecting", "fixing", "screw connection" and the like should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integrated; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through an intermediate medium; it can be the communication inside two elements or the interaction relationship between two elements, unless otherwise explicitly limited, the above-mentioned terms in the utility model can be understood according to the specific meaning of the above-mentioned terms in the utility model by the person skilled in the art according to the specific situation.
[0026] The above merely describes preferred embodiments of the present application and is not intended to limit the present application. Although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art will appreciate that the technical solutions recorded in the foregoing embodiments can be modified, or some technical features thereof can be replaced equivalently. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. An integrated nanoliter injection pump, comprising a pump body (1), a front end of the pump body (1) is provided with an interface (2), a push needle body (17) is through-penetrated in the interface (2), characterized in that: The interface (2) is provided with a fixed needle assembly, the fixed needle assembly comprises a joint (3), a sealing gasket (4) and a gasket (5), the joint (3) is sleeved and fixed outside the interface (2), the joint (3) is internally provided with a fixed cavity (6), one end of the fixed cavity (6) is connected with a mounting cavity (7), the sealing gasket (4) is mounted in the mounting cavity (7), the gasket (5) is abutted on the interface (2) of the pump body (1) through the sealing gasket (4), the inner wall of the fixed cavity (6) is fixedly provided with an annular air bag (8), the joint (3) is provided with an air exchange channel (9) on both sides of the annular air bag (8), the two air exchange channels (9) are both communicated with the inside of the annular air bag (8), one end of the air exchange channel (9) is provided with a control air valve (10), the other end of the air exchange channel (9) is connected with an air pipe (11), one end of the air pipe (11) is provided with an inflating air bag (12), the air pipe (11) is provided with a one-way air valve (13), the center of the joint (3), the sealing gasket (4) and the gasket (5) is provided with a limiting channel.
2. The integrated nanoliter syringe pump of claim 1, wherein: The limiting channel in the center of the joint (3), the sealing gasket (4) and the gasket (5) and the center of the annular air bag (8) are arranged in a line, and the push needle body (17) of the pump body (1) penetrates the limiting channel in the center of the joint (3), the sealing gasket (4) and the gasket (5) and the center of the annular air bag (8) respectively.
3. The integrated nanoliter syringe pump of claim 2, wherein: The limiting channel comprises a capillary channel (14) and a push needle channel (15), and the capillary channel (14) and the push needle channel (15) are communicated.
4. The integrated nanoliter syringe pump of claim 3, wherein: One end of the capillary channel (14) is connected with the push needle channel (15), and the other end of the capillary channel (14) is provided with a guide opening (16).
5. The integrated nanoliter syringe pump of claim 1, wherein: The inflating air bag (12), the air pipe (11) and the one-way air valve (13) are fixed to the outer wall of the joint (3).