Microorganism separation culture device

By designing a microbial isolation and culture device that combines a rotating stage and a power mechanism, the problem of uneven distribution of culture medium was solved, and uniform addition of culture medium and efficient culture were achieved.

CN223522504UActive Publication Date: 2025-11-07QINGDAO HUASHENG SHIDA BIOPHARMACEUTICAL CO LTD
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Patent Information

Application Number
CN202422921512.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-11-28
Publication Date
2025-11-07
Estimated Expiration
2034-11-28

AI Technical Summary

Technical Problem

In existing microbial isolation and culture devices, when nutrients in the culture medium are consumed during long-term cultivation, the need to add culture medium can easily lead to uneven distribution due to manual operation.

Method used

A microbial isolation and culture device was designed, comprising a rotating stage, a fixed stage, a culture dish, and a power mechanism. Through the cooperation of the upper and lower wedges, the rotating stage drives the culture dish to rotate and shake. Combined with water bath heating and a detachable microporous filter membrane, uniform distribution of the culture medium is achieved.

Benefits of technology

This method achieves uniform distribution of the culture medium in the petri dish, improves culture efficiency and effectiveness, and reduces the unevenness caused by manual operation.

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Abstract

The utility model relates to the technical field of microorganism culture, in particular to a microorganism separation culture device which comprises a culture table, a plurality of mounting grooves are distributed in the culture table in an array manner; a culture unit is arranged in the mounting groove; the culture unit comprises a base, a rotary table, a fixed table, culture dishes and a power mechanism; a mounting cavity is formed in the base, and a plurality of lower wedge blocks are fixed to the upper end face of the base in the circumferential direction. The rotating table is arranged on the base, and a plurality of upper wedge blocks are fixed to the lower end face of the rotating table in the circumferential direction. A clamping groove is formed in the upper end face of the fixed table, and the fixed table is fixedly connected to the rotating table. The culture dish is clamped and fixed in the clamping groove; and the power mechanism is arranged in the mounting cavity. The power device drives the rotary table to drive the culture dishes to rotate; when the rotating table rotates, an upper wedge block on the lower end surface of the rotating table is matched with a lower wedge block on the upper end surface of the base to drive the rotating table to drive the culture dish to shake, so that the culture solution added into the culture dish is uniformly distributed.
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Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of microorganism culture, and particularly relates to a microorganism separation and culture device. BACKGROUND

[0002] Microorganism separation and culture is a basic technology in microbiology, aiming to separate specific microorganisms from complex microbial populations. This technology includes sample collection, processing, microorganism separation, culture, identification, and preservation. Microorganism separation and culture provides an important means for the study of microbiology and the role of microorganisms in food, medicine, agriculture, and other fields, enabling people to better understand the physiological characteristics, metabolic pathways, and ecological functions of microorganisms, thereby providing new ideas and methods for solving various biological problems.

[0003] In the prior art, for example, a Chinese patent with publication number CN218491696U, entitled "Microorganism separation and culture device", includes a main culture component and an auxiliary culture component. Through the cooperation of the main culture component and the auxiliary culture component, the microorganism culture device can separate an independent space and work independently, and can culture and process two kinds of microorganisms at a time. Compared with related technical fields and similar technical solutions, when the microorganism separation and culture time is relatively long, the nutrients in the culture medium will be gradually consumed, and it is necessary to add culture solution to the culture dish in a timely manner. However, when the culture solution is manually shaken, the distribution of the culture solution may be uneven due to different force and frequency of the person. SUMMARY

[0004] The utility model provides a microorganism separation and culture device to solve the above problems.

[0005] The utility model adopts the following technical scheme: a microorganism separation and culture device, including a culture table, a plurality of installation grooves are arranged on the culture table in an array, a culture unit is arranged in the installation groove, the culture unit includes a base, a rotating table, a fixed table, a culture dish and a power mechanism, a plurality of lower wedge blocks are fixed uniformly on the upper end face of the base along the circumference, an installation cavity is arranged in the base, a heating device is arranged above the installation cavity, the rotating table is arranged on the base, a plurality of upper wedge blocks are fixed uniformly on the lower end face of the rotating table along the circumference, the upper wedge blocks abut against the upper end face of the base, and the lower wedge blocks abut against the lower end face of the rotating table, a threaded hole is arranged at the center of the upper end face of the rotating table, the fixed table is arranged on the rotating table, a clamping groove is arranged on the upper end face of the fixed table, a threaded hole is arranged at the center of the lower end face of the fixed table, the threaded hole is threadedly connected with the threaded hole, the culture dish is clamped in the clamping groove, and the power mechanism is arranged in the installation cavity to drive the rotating table to rotate the culture dish, and the rotating table drives the culture dish to shake up and down under the cooperation of the upper wedge blocks and the lower wedge blocks.

[0006] Further, the power mechanism comprises a transmission device and a driving device; the transmission device comprises a matching hole, a rotating rod, a spline and a first bevel gear; the matching hole is arranged at the center of the lower end surface of the rotating table; the rotating rod is vertically arranged and rotationally connected with the bottom of the base; the rotating rod is rotationally connected with the top of the base through a sealing bearing; the spline is fixedly installed on the upper end of the rotating rod; the spline is slidingly matched with the matching hole; and the first bevel gear is fixedly installed on the rotating rod.

[0007] Further, the driving device comprises a driving motor and a second bevel gear; the driving motor is fixedly installed in the mounting cavity of the base; the second bevel gear is fixedly installed on the output shaft of the driving motor and engaged with the first bevel gear; the driving motor outputs power through the second bevel gear and the first bevel gear to the rotating rod; the rotating rod is slidingly matched with the matching hole through the spline to drive the rotating table to rotate; when the rotating body rotates, the upper wedge block rotates along the circumference and collides with the lower wedge block to make the rotating table vibrate.

[0008] Further, the heating device comprises a heating cylinder and an electric heating wire; the heating cylinder is coaxially fixed on the upper end of the base and sleeved on the outside of the rotating table; the electric heating wire is arranged on the upper end surface of the base; water is added in the heating cylinder to perform water bath heating on the culture dish so as to keep the temperature in the culture dish suitable for microbial culture. Further, the upper end of the culture dish is provided with an upper cover; the upper cover is buckled on the culture dish; an air exchange hole is arranged at the center of the upper cover; a microporous filter film is detachably arranged above the air exchange hole; a liquid adding hole is arranged at one side of the center of the upper cover; a liquid adding plug is detachably arranged in the liquid adding hole; and a rubber ring is fixedly installed on the inner side of the upper cover.

[0009] Further, the microporous filter film is pasted on the upper cover through a bottom adhesive tape; when it is replaced, only the microporous filter film to be replaced needs to be torn off and a new microporous filter film is pasted.

[0010] Beneficial effects are that the power mechanism drives the rotating table to rotate and drives the rotating table to vibrate up and down with the culture dish through the cooperation of the upper wedge block and the lower wedge block, so that the culture solution added into the culture dish is uniformly distributed. BRIEF DESCRIPTION OF DRAWINGS

[0011] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or prior art description, obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor under the premise of the drawings.

[0012] Figure 1 The structural schematic view of an embodiment of the microbial separation and culture device of the present application;

[0013] Figure 2 The structural schematic view of the culture unit of the embodiment of the present application;

[0014] Figure 3 The bottom view of the rotating table of the culture unit of the embodiment of the present application;

[0015] Figure 4 The sectional view at A-A in the above figure; Figure 3 The sectional view at A-A in the above figure;

[0016] Figure 5 The structural schematic view of the base of the culture unit of the embodiment of the present application.

[0017] In the figure: 001, culture table; 100, base; 110, lower wedge block; 120, rotating rod; 130, spline; 140, first bevel gear; 150, driving motor; 160, second bevel gear; 170, heating wire; 180, heating cylinder; 200, rotating table; 210, matching hole; 220, upper wedge block; 230, threaded hole; 300, fixed table; 310, stud; 400, culture dish; 500, upper cover; 510, microporous filter membrane; 520, liquid adding plug; 530, rubber ring. DETAILED DESCRIPTION

[0018] In the description of the present application, it should be noted that, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connecting" should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integrally connected; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through intermediate medium, or the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0019] An embodiment of the microbial separation and culture device of the present application, such as Figures 1 to 5The utility model discloses a kind of microbial separation culture devices, including culture table 001;Multiple installation slots are distributed with array on culture table 001;Culture unit is equipped in installation slot;Culture unit includes base 100, rotating table 200, fixed table 300, culture dish 400 and power mechanism;Multiple lower wedges 110 are uniformly fixed with on end surface of base 100 along circumference, installation cavity is equipped in base 100, heating device is equipped above installation cavity;By being provided with multiple culture unit, multiple culture dishes 400 can be placed once, and multiple microorganisms can be separated and cultured once.

[0020] Rotating table 200 is arranged on base 100, and multiple upper wedges 220 are uniformly fixed with on lower end surface of rotating table 200 along circumference, the upper wedge 220 and the end surface of base 100 are abutted, and the lower wedge 110 and the lower end surface of rotating table 200 are abutted;Threaded hole 230 is arranged at the center of the upper end surface of rotating table 200;Fixed table 300 is arranged on rotating table 200, and clamping groove is arranged on the upper end surface of fixed table 300;Screw column 310 is fixed at the center of the lower end surface of fixed table 300, and screw column 310 is threadedly connected with threaded hole 230;Different fixed tables 300 are used to replace different culture dishes 400 according to different sizes.

[0021] Culture dish 400 is clamped in clamping groove;Power mechanism is arranged in installation cavity, and is used to drive rotating table 200 to rotate culture dish 400, and rotating table 200 drives culture dish 400 to shake up and down under the cooperation of upper wedge 220 and lower wedge 110.

[0022] Power mechanism includes transmission device and driving device;Transmission device includes cooperation hole 210, rotating rod 120, spline 130 and first bevel gear 140;Cooperation hole 210 is arranged at the center of the lower end surface of rotating table 200;Rotating rod 120 is vertically arranged, and rotating rod 120 is rotatably connected with the bottom of base 100;Rotating rod 120 is rotatably connected with the top of base 100 through sealing bearing;Spline 130 is fixedly installed on the upper end of rotating rod 120;Spline 130 is slidably connected with cooperation hole 210;First bevel gear 140 is fixedly installed on rotating rod 120;Driving device includes driving motor 150 and second bevel gear 160, and driving motor 150 is fixedly installed in installation cavity of base 100;Second bevel gear 160 is fixedly installed on the output shaft of driving motor 150, and second bevel gear 160 is engaged with first bevel gear 140;Driving motor 150 outputs power through second bevel gear 160 and first bevel gear 140 to rotating rod 120, and rotating rod 120 drives rotating table 200 to rotate through the sliding connection between spline 130 and cooperation hole 210, and rotating body drives upper wedge 220 to rotate along circumference when rotating, and upper wedge 220 rotates along circumference to collide with lower wedge 110, so that rotating table 200 shakes.

[0023] The heating device comprises a heating cylinder 180, and an electric heating wire 170, the heating cylinder 180 is coaxially fixed on the upper end of the base 100 and is sleeved outside the rotating table 200, the electric heating wire 170 is arranged on the upper end surface of the base 100, water is added in the heating cylinder 180, so that the culture dish 400 is heated by water bath, and the culture dish 400 is kept at a temperature suitable for microbial culture.

[0024] The upper end of the culture dish 400 is provided with an upper cover 500, the upper cover 500 is buckled on the culture dish 400, the center of the upper cover 500 is provided with an air exchange hole, a microporous filter membrane 510 is detachably arranged above the air exchange hole, a liquid adding hole is arranged on one side of the center of the upper cover 500, and a liquid adding plug 520 is detachably arranged in the liquid adding hole, and a rubber ring 530 is fixedly arranged on the inner side of the upper cover 500.

[0025] The microporous filter membrane 510 is pasted on the upper cover 500 through a bottom adhesive tape, and when the microporous filter membrane 510 needs to be replaced, the microporous filter membrane 510 to be replaced is only torn off and a new microporous filter membrane 510 is pasted.

[0026] In combination with the above embodiment, the use principle and working process of the utility model are as follows: when the microorganism is separated and cultured, the upper cover 500 matched with the size of the culture dish 400 is selected and the fixing table 300 is selected; the microporous filter membrane 510 to be replaced is torn off, and a new microporous filter membrane is pasted on the upper cover 500; the fixing table 300 is fixed on the rotating table 200; after the culture dish 400 is coated with a microbial sample, the upper cover 500 is buckled on the culture dish 400, and the culture dish 400 is placed in the clamping groove of the fixing table 300; water is added into the heating cylinder 180, and the heating device heats the water in the heating cylinder 180 to a temperature suitable for the survival of the microorganism, so that the culture dish 400 is heated by water bath, and the culture dish 400 is more uniformly heated.

[0027] The microorganism separation and culture need a long time, when the culture liquid needs to be added into the culture dish 400, the liquid adding plug 520 in the liquid adding hole is pulled out, the culture liquid is added into the culture dish 400 from the liquid adding hole; then the power mechanism is powered on to start working, the driving motor 150 drives the power to the second bevel gear 160 at the output end; the second bevel gear 160 is engaged with the first bevel gear 140 to drive the power to the first bevel gear 140; the first bevel gear 140 drives the rotating shaft to rotate, the rotating shaft drives the top end spline 130 to rotate; the spline 130 is slidably matched with the matching hole 210 in the center of the lower end surface of the rotating table 200, drives the rotating table 200 to rotate, and drives the upper wedge block 220 to rotate circumferentially when the rotating table 200 rotates, so that the rotating table 200 is shaken when the upper wedge block 220 rotates circumferentially and touches the lower wedge block 110; the rotating table 200 transmits the circumferential rotation and shaking to the culture dish 400 through the fixing table 300, so that the culture dish 400 rotates circumferentially and shakes, and the culture liquid added in the culture dish 400 is more uniformly distributed.

[0028] The above merely describes preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A microbial isolation culture device, characterized by: The culture table (001) is provided with a plurality of installation slots arranged in an array; a culture unit is arranged in the installation slot; the culture unit comprises a base (100), a rotating table (200), a fixed table (300), a culture dish (400) and a power mechanism; A plurality of lower wedge blocks (110) are fixed on the upper end surface of the base (100) in a circumferential direction; an installation cavity is arranged in the base (100); a heating device is arranged above the installation cavity; The rotating table (200) is arranged on the base (100); a plurality of upper wedge blocks (220) are fixed on the lower end surface of the rotating table (200) in a circumferential direction; the upper wedge blocks (220) abut against the upper end surface of the base (100); the lower wedge blocks (110) abut against the lower end surface of the rotating table (200); a threaded hole (230) is arranged at the center of the upper end surface of the rotating table (200); the fixed table (300) is arranged on the rotating table (200); a clamping groove is arranged on the upper end surface of the fixed table (300); a stud (310) is fixed at the center of the lower end surface of the fixed table (300); the stud (310) is threadedly connected with the threaded hole (230); The culture dish (400) is clamped in the clamping groove; The power mechanism is arranged in the installation cavity; the power mechanism drives the rotating table (200) to rotate and drives the culture dish (400) to shake up and down under the cooperation of the upper wedge blocks (220) and the lower wedge blocks (110).

2. The microbial isolation culture device of claim 1, wherein: The power mechanism comprises a transmission device and a driving device; the transmission device comprises a matching hole (210), a rotating rod (120), a spline (130) and a first bevel gear (140); the matching hole (210) is arranged at the center of the lower end surface of the rotating table (200); the rotating rod (120) is arranged vertically; the rotating rod (120) is rotatably connected with the bottom of the base (100); the rotating rod (120) is rotatably connected with the top of the base (100) through a sealing bearing; the spline (130) is fixedly arranged on the upper end of the rotating rod (120); the spline (130) is slidably connected with the matching hole (210) in an up-down direction; the first bevel gear (140) is fixedly arranged on the rotating rod (120).

3. The microbial isolation culture device of claim 2, wherein: The driving device comprises a driving motor (150) and a second bevel gear (160); the driving motor (150) is fixedly arranged in the installation cavity of the base (100); the second bevel gear (160) is fixedly arranged on the output shaft of the driving motor (150); the second bevel gear (160) is engaged with the first bevel gear (140).

4. The microbial isolation culture device of claim 3, wherein: The heating device comprises a heating cylinder (180) and an electric heating wire (170); the heating cylinder (180) is coaxially fixed on the upper end of the base (100) and is sleeved outside the rotating table (200); the electric heating wire (170) is arranged on the upper end surface of the base (100).

5. The microbial isolation culture device of claim 1, wherein: The upper end of the culture dish (400) is provided with an upper cover (500); the upper cover (500) is buckled on the culture dish (400), the center of the upper cover (500) is provided with an air exchange hole, the upper air exchange hole is detachably provided with a microporous filter membrane (510), one side of the center of the upper cover (500) is provided with a liquid adding hole, the liquid adding hole is detachably provided with a liquid adding plug (520), and the inner side of the upper cover (500) is fixedly installed with a rubber ring (530).

6. The microbial isolation culture device of claim 5, wherein: The microporous filter membrane (510) is pasted on the upper cover (500) through a bottom adhesive tape.

Citation Information

Patent Citations

  • Microorganism separation culture device

    CN218491696U