Microorganism anaerobic culture system

Abstract

The utility model provides a microorganism anaerobic culture system which comprises a vacuum pump, an oxygen-free bottle, a microorganism culture test tube, a needle head, a pressure gauge, an air pipe and a cross air pipe communication interface, and the vacuum pump, the oxygen-free bottle, the needle head and the pressure gauge are connected with the cross air pipe communication interface through the air pipe to form a communication device. The microorganism culture test tube comprises a rubber plug for sealing, and the needle penetrates through the rubber plug to be inserted into the microorganism culture test tube and is kept sealed. The microorganism anaerobic culture system is low in preparation cost and simple in structure, and can conveniently meet the preparation of an anaerobic microorganism culture environment in a laboratory.

Description

TECHNICAL FIELD

[0001] The utility model belongs to anaerobic culture system, especially designs a kind of microbial anaerobic culture system. TECHNICAL BACKGROUND

[0002] Anaerobic microorganisms are a class of microorganisms that can grow normally in anoxic or low-oxygen environments. Facultative anaerobic microorganisms (facultative anaerobes) are microorganisms that can grow in both aerobic and anaerobic conditions. They are widely present in nature, including the environment, soil, water sources and animals, and have important influence on human health and ecological systems. Most anaerobic microorganisms are bacteria, and a few are actinomycetes and mycoplasmas. They have different tolerance to oxygen and can be divided into obligate anaerobes, microaerophilic anaerobes and facultative anaerobes. Obligate anaerobes are extremely sensitive to oxygen and will die if exposed to aerobic environment; while facultative anaerobes can grow in aerobic or anaerobic conditions. The culture of anaerobic microorganisms requires special environment, such as the use of anaerobic jars, anaerobic bags or anaerobic glove boxes, etc., to exclude oxygen and create anaerobic conditions. Anaerobic microorganisms have very high requirements for culture environment, and often fail in laboratory culture because of the inability to prepare anaerobic environment or the failure to achieve anaerobic conditions. Anaerobic culture system has become an indispensable tool for microbiological research. However, the existing anaerobic culture environment preparation methods have high cost, inconvenient operation or cannot meet the absolute anaerobic requirements, and have not been completely popularized in practical application. SUMMARY

[0003] The utility model provides a kind of microbial anaerobic culture system, which is simple in structure, low in manufacturing cost and convenient for laboratory use.

[0004] A microbial anaerobic culture system includes a vacuum pump, an oxygen-free gas cylinder, a microbial culture test tube, a needle, a pressure gauge, an air pipe and a cross air pipe communication interface. The vacuum pump, the oxygen-free gas cylinder, the needle and the pressure gauge are connected to the cross air pipe communication interface through the air pipe, forming a communication device. The microbial culture test tube is sealed with a rubber plug, and the needle is inserted into the microbial culture test tube through the rubber plug while maintaining the seal.

[0005] As a specific implementation, the vacuum pump is connected to the cross air pipe communication interface through the air pipe, with a vacuum pump valve in between.

[0006] As a specific implementation, the oxygen-free gas cylinder is provided with a pressure relief valve, which is connected to the cross air pipe communication interface through the air pipe, with an oxygen-free gas cylinder valve in between.

[0007] As a specific implementation, the microbial culture test tube includes a rubber plug, which is used to seal the microbial culture test tube.

[0008] As a specific implementation, the needle has an air tube connected to a cross-shaped air tube interface, with a double-entry air tube buckle and an air valve in the middle.

[0009] As a specific implementation, the needle can penetrate the rubber stopper and enter the microbial culture tube while remaining sealed.

[0010] Beneficial effects: Compared with the prior art, this utility model has low cost and simple structure, and can meet the requirements for the preparation of laboratory liquid anaerobic culture medium. Attached Figure Description

[0011] Figure 1 This is a schematic diagram of the anaerobic system of this utility model. Detailed Implementation

[0012] The present invention will be further described below with reference to the accompanying drawings.

[0013] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances. Example

[0014] A microbial anaerobic culture system, such as Figure 1 As shown, it includes a vacuum pump 1, an oxygen-free cylinder 2, a microbial culture test tube 3, a needle 4, a pressure gauge 5, a trachea 6, and a cross-shaped trachea connector 7.

[0015] Vacuum pump 1 is connected to cross-shaped air pipe interface 7 via air pipe 6, with vacuum pump valve 101 installed in between.

[0016] The oxygen-free cylinder 2 is equipped with a pressure relief valve 201, which is connected to the cross-shaped air tube interface 7 through the air tube 6, and an oxygen-free cylinder valve 202 is set in the middle.

[0017] The microbial culture test tube 3 includes a rubber stopper 301, which seals the microbial culture test tube 3 tightly.

[0018] The needle 4 has an air tube 6 connected to a cross-shaped air tube interface 7, with a double air tube buckle 401 and an air valve 402 in the middle.

[0019] Pressure gauge 5 is connected to cross-shaped airway connector 7 via airway 6.

[0020] The needle 4 can pass through the rubber plug 301 to enter the inside of the microorganism culture test tube 3 and keep airtight.

[0021] The use principle of the above-mentioned microorganism anaerobic culture system is as follows:

[0022] According to the requirement of culturing microorganisms, the appropriate liquid culture medium is added into the microorganism culture test tube 3, the rubber plug 301 is covered, and the needle 4 is inserted into the microorganism culture test tube 3 through the rubber plug 301. The oxygen-free gas cylinder 2 can be filled with inert gases such as nitrogen and carbon dioxide, the vacuum pump valve 101, the oxygen-free gas cylinder valve 202 and the gas valve 402 are in the closed state, the pressure relief valve 201 on the oxygen-free gas cylinder 2 is opened, the oxygen-free gas cylinder valve 202 and the gas valve 402 are sequentially opened, when the pressure gauge 5 shows a stable number, the oxygen-free gas cylinder valve 202 is closed, and the pressure gauge 5 is waited for 1 minute, if the pressure gauge 5 shows a constant number, it indicates that the gas supplementing link is not leaking. The vacuum pump 1 is started, the vacuum pump valve 101 is opened, when the pressure gauge 5 shows a number close to-0.1 MPa, the vacuum pump valve 101 is closed, and the pressure gauge 5 is waited for 1 minute, if the pressure gauge 5 shows a constant number, it indicates that the gas extraction link is not leaking.

[0023] If there is leakage in the gas supplementing and gas extracting links, the airtightness needs to be checked and the airtightness problem needs to be solved, and if they are all not leaking, the subsequent steps can be continuously operated.

[0024] The oxygen-free gas cylinder valve 202 is opened, the oxygen-free gas in the microorganism culture tube 3 is supplemented, and the oxygen-free gas cylinder valve 202 is closed. The vacuum pump valve 101 is opened, the gas is extracted to a vacuum state, the vacuum pump valve 101 is closed, and the gas replacement in the microorganism culture tube 3 is completed.

[0025] After the above-mentioned gas replacement step is cycled for 3 times, the oxygen-free gas in the microorganism culture tube 3 is supplemented after the oxygen-free gas cylinder valve 202 is opened, the gas valve 402 is closed, the double-way air pipe buckle 401 is pulled off, the pressure gas in the microorganism culture tube 3 is discharged to restore normal pressure, and the needle 4 is immediately pulled out, that is, the preparation of the oxygen-free environment of the microorganism culture tube 3 is completed, the microorganism culture tube 3 can be placed in a sterilization pot for high-temperature sterilization, the strain is inoculated into the microorganism culture tube 3 by using a sterile syringe, and anaerobic culture is carried out.

[0026] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A microbial anaerobic culture system, comprising a vacuum pump, an oxygen-free gas cylinder, a microbial culture test tube, a needle, a pressure gauge, a gas pipe, and a cross pipe communication interface, the vacuum pump, the oxygen-free gas cylinder, the needle, and the pressure gauge being connected to the cross pipe communication interface through the gas pipe, forming a communication device, the microbial culture test tube being sealed by a rubber plug, the needle being inserted into the microbial culture test tube through the rubber plug and keeping the seal.

2. The microorganism anaerobic culturing system according to claim 1, characterized by, The vacuum pump is connected to the cross pipe communication interface through the gas pipe, with a vacuum pump valve being arranged in between.

3. The microorganism anaerobic culturing system of claim 1, wherein, The oxygen-free gas cylinder is provided with a pressure relief valve and is connected to the cross pipe communication interface through the gas pipe, with an oxygen-free gas cylinder valve being arranged in between.

4. The microorganism anaerobic culturing system of claim 1, wherein, The microbial culture test tube comprises a rubber plug for sealing the microbial culture test tube.

5. The microorganism anaerobic culturing system of claim 1, wherein, The needle is connected to the cross pipe communication interface through the gas pipe, with a double gas pipe buckle and a gas valve being arranged in between.

6. The microorganism anaerobic culturing system of claim 1, wherein, The needle can be inserted into the microbial culture test tube through the rubber plug and keep the seal.

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