Centrifugal device for cells and proteins

Abstract

The utility model provides a centrifugal device for cells and proteins, and relates to cell or protein centrifugation. The centrifugal device for the cells and the proteins comprises a centrifugal tube, a bracket arranged in the centrifugal tube and a filter membrane tank which is arranged in the bracket, wherein the notch direction of the filter membrane tank faces the top of the bracket; the filter membrane tank is suitable for loading a filter membrane; a plurality of groups of side wall filtering assemblies which are sequentially arranged in the circumferential direction of the bracket are arranged on the side wall of the bracket, and a bottom filtering assembly is arranged at the bottom of the bracket; and each group of side wall filtering components comprises a plurality of side wall filtering holes which are sequentially arranged along the extension direction of the bracket. According to the utility model, the probability that macromolecules and particulate matters are accumulated on the surface of the filter membrane in the filtering process can be reduced, the shearing force on biomolecules in the centrifugal process is reduced, the molecular activity is prevented from being greatly reduced, and the filtering efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the centrifugal field of cell or protein, especially a centrifugal device for cell and protein. BACKGROUND

[0002] The commonly used ultrafiltration centrifugal tube on the market adopts a tangential flow filtering mode in the centrifugal stage, for example, an ultrafiltration centrifugal tube with the patent number CN205253154U, tangential flow filtration (TFF) is a membrane separation technology, and the core principle thereof is to drive fluid to flow along the membrane surface in parallel instead of vertically through the membrane by using pressure. This flow mode is similar to the "cutting" of fluid on the membrane surface, and thus is named "tangential flow".

[0003] The traditional tangential flow filtering mode has three main shortcomings: 1. During the tangential flow filtration process, macromolecules and particulate matter are easily accumulated on the membrane surface, which leads to reduced filtering efficiency and increased operation cost; 2. In the tangential flow filtration, the tangential flow of fluid may generate shear force on some sensitive biological molecules, which leads to the decrease of the activity of the molecules; 3. In the tangential flow filtration, the transmembrane pressure is relatively high, and the excessively high transmembrane pressure may lead to the deformation or damage of the membrane pores, which affects the filtering efficiency; 4. In the tangential flow filtration, the membrane pores are very easy to be blocked for high-concentration cell and protein samples, which reduces the filtering effect, wastes the samples, and increases the centrifugal force time.

[0004] In summary, there is a need for a centrifugal device that does not use the tangential flow mode to filter and concentrate cell and protein solutions. CONTENT OF THE UTILITY MODEL

[0005] In order to solve the above problems, the utility model provides a centrifugal device for cell and protein, which can reduce the probability of macromolecules and particulate matter accumulating on the surface of the filter membrane in the filtering process, reduce the shear force generated on the biological molecules in the centrifugal process, prevent the significant decrease of the activity of the molecules, and improve the filtering efficiency.

[0006] The centrifugal device for cell and protein provided by the utility model comprises a centrifugal tube, a support arranged in the centrifugal tube, and a filter membrane groove arranged in the support and having a slot direction towards the top of the support, and the filter membrane groove is suitable for loading a filter membrane; a plurality of side wall filter assemblies arranged in the circumferential direction of the support are arranged on the side wall of the support, and a bottom filter assembly is arranged at the bottom of the support; each side wall filter assembly comprises a plurality of side wall filter holes arranged in the extension direction of the support.

[0007] In an implementable embodiment, the side wall of the support is gradually inclined inwardly in the direction from the top of the support to the bottom of the support, and the inclination angle a of the side wall of the support is 30°-60°.

[0008] In an embodiment, the size of the side wall filter holes gradually decreases from the top of the bracket to the bottom of the bracket.

[0009] In some embodiments, the length of the bracket is 80-100 mm; and / or, the thickness of the bracket is 1-3 mm; and / or, the inner diameter of the bracket is 30-35 mm; and / or, the outer diameter of the bracket is 35-40 mm; and / or, the interval angle between two adjacent groups of the side wall filter assemblies is the same; and / or, the number of the side wall filter assemblies is 5-10; and / or, the interval between two adjacent side wall filter holes in the same group of the side wall filter assemblies is the same; and / or, the number of the side wall filter holes in each group of the side wall filter assemblies is 5-10; and / or, the shape of the side wall filter holes is selected from any one of a circle, an ellipse or a rectangle.

[0010] In an embodiment, the shape of the side wall filter holes is a rectangle, the length of the side wall filter holes is 5-15 mm, the width of the side wall filter holes is 1-11 mm, and the interval between two adjacent side wall filter holes in the same group of the side wall filter assemblies is 3-8 mm.

[0011] In an embodiment, the bottom filter assembly comprises a ring-shaped filter channel and a circular filter channel surrounded by the ring-shaped filter channel; a plurality of first partitions are arranged in the ring-shaped filter channel, and a secondary ring-shaped filter channel is formed between two adjacent first partitions; a plurality of second partitions are arranged in the circular filter channel, and a secondary circular filter channel is formed between two adjacent second partitions.

[0012] In some embodiments, the inner diameter of the ring-shaped filter channel is 15-25 mm, and the outer diameter of the ring-shaped filter channel is 25-35 mm; and / or, the number of the first partitions is 4-8; and / or, the diameter of the circular filter channel is 10-20 mm; and / or, the number of the second partitions is 4-8; and / or, the interval between two adjacent first partitions is the same; and / or, the interval between two adjacent second partitions is the same; and / or, the width of the first partitions is 1-3 mm; and / or, the width of the second partitions is 1-3 mm.

[0013] In an embodiment, further comprising an upper anti-collision ring and a lower anti-collision ring arranged around the side wall of the bracket, the upper anti-collision ring is close to the top of the bracket, and the lower anti-collision ring is close to the bottom of the bracket; the outer diameters of the upper anti-collision ring and the lower anti-collision ring are the same.

[0014] In an embodiment, the interval between the upper anti-collision ring and the lower anti-collision ring and the inner wall of the centrifugal tube is 1-3 mm.

[0015] In some possible implementations, the upper anti-collision ring has a height of 3-5 mm; and / or, the lower anti-collision ring has a height of 3-5 mm.

[0016] The centrifugal device for cells and proteins has the following beneficial effects:

[0017] 1) The centrifugal device of the utility model discloses abandons the traditional tangential flow filter form, can reduce the probability of macromolecule and particulate matter in the filtration to accumulate on the surface of the filter membrane, reduce the shear force generated to the biological molecules in the centrifugal process, prevent the sharp decline of molecular activity, improve the filtration efficiency; in addition, the centrifugal filter area of the utility model is greatly increased, can more effectively realize the purpose of solid-liquid separation, concentration, desalination and washing, and the influence on the activity of the product is greatly reduced.

[0018] 2) The upper anti-collision ring and the lower anti-collision ring can make the support keep a good center of gravity in the centrifugal stage, cannot swing greatly in the centrifugal tube, and improve the safety of the centrifugal stage. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is an exploded view of the utility model.

[0020] Figure 2 It is a structural schematic view of the support in the utility model.

[0021] Figure 3 It is a front view of the support in the utility model.

[0022] Figure 4 It is a bottom view of the support in the utility model.

[0023] Figure 5 It is a structural schematic view of the utility model after assembly.

[0024] REFERENCE NUMERALS

[0025] Centrifugal tube 1

[0026] Support 2

[0027] Upper anti-collision ring 21

[0028] Lower anti-collision ring 22

[0029] Filter membrane groove 3

[0030] Side wall filter assembly 4

[0031] Side wall filter hole 41

[0032] Bottom filter assembly 5

[0033] Annular filter channel 51

[0034] Secondary circular filter passage 51.1

[0035] First partition 51.2

[0036] Cylindrical filter passage 52

[0037] Secondary cylindrical filter passage 52.1

[0038] Second partition 52.2 DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application. In the description of the present application, it should be noted that the orientations or positional relationships indicated by the terms "left side", "right side", "upper side", "lower side", "upper", "lower" and the like are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application. In addition, the terms "first" and "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0040] In the description of the present application, it should be noted that, unless otherwise explicitly specified and limited, the terms "mounting", "connection", "connection" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the communication inside two elements. For those of ordinary skill in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0041] In addition, in the description of the present application, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0042] The centrifugal device for cells and proteins provided in the embodiments of the present application, with reference to Figures 1-2The utility model discloses a centrifugal device for cells and proteins, including centrifugal tube 1, the support 2 of being located in centrifugal tube 1 and the filter membrane slot 3 of being located in support 2 and the notch direction is towards support 2 top, the filter membrane slot 3 is suitable for loading filter membrane in. The side wall of support 2 is equipped with multiple groups of side wall filter components 4 in the circumferential direction of support 2 sequentially, and support 2 is generally cylindrical. The bottom of support 2 is equipped with bottom filter component 5, and each group of side wall filter components 4 includes multiple side wall filter holes 41 sequentially arranged in the extension direction of support 2, and the extension direction of support 2 is the axial direction of support 2. In use, the filter membrane is first placed in the filter membrane slot 3 of support 2, similar to the way of a garbage bag, and the filter membrane is as far as possible to be attached to the filter membrane slot 3. Then, the support 2 with the filter membrane is placed in the centrifugal tube 1, and the sample solution is poured into the filter membrane. Finally, the centrifugal tube 1 is closed and placed in the centrifuge for centrifugation. After the centrifugation stage is completed, the filter membrane is removed. The solid residue wrapped by the filter membrane is the concentrated liquid after the sample solution is separated. The utility model discards the traditional tangential flow filtration form, can reduce the probability of macromolecules and particulate matter accumulating on the surface of the filter membrane during filtration, reduce the shear force generated on biological molecules during centrifugation, prevent a substantial decrease in molecular activity, and improve filtration efficiency.

[0043] In an embodiment, the length of the support 2 is 80-100 mm, preferably 90 mm. In an embodiment, the thickness of the support 2 is 1-3 mm, preferably 2 mm. In an embodiment, the inner diameter of the support 2 is 30-35 mm, preferably 30 mm. In an embodiment, the outer diameter of the support 2 is 35-40 mm, preferably 35 mm. In an embodiment, the spacing angle between adjacent two groups of side wall filter components 4 is the same, which can be referred to Figure 4 In an embodiment, the number of side wall filter components 4 is 5-10, preferably 6. In an embodiment, the spacing between adjacent two side wall filter holes 41 in the same group of side wall filter components 4 is the same. In an embodiment, the number of side wall filter holes 41 in each group of side wall filter components 4 is 5-10, preferably 6. In an embodiment, the shape of the side wall filter hole 41 is selected from any one of a circle, an ellipse or a rectangle, preferably a rectangle. In an embodiment, the shape of the side wall filter hole 41 is a rectangle, the length of the side wall filter hole 41 is 5-15 mm, preferably 10 mm, the width of the side wall filter hole 41 is 1-11 mm, preferably 6 mm, and the spacing between adjacent two side wall filter holes 41 in the same group of side wall filter components 4 is 3-8 mm, preferably 5 mm.

[0044] In the centrifugal device for cells and proteins provided by the utility model embodiment,Figures 1-3 and Figure 5 The side wall of the support 2 is gradually inclined inward in the direction from the top of the support 2 to the bottom of the support 2, and the inclination angle a of the side wall of the support 2 is 30-60°, preferably 60°. When the side wall of the support 2 is not inclined, the volume of the sample solution wrapped by the filter membrane in the support 2 does not form a gradient, and when the side wall of the support 2 is inclined, the volume of the sample solution wrapped by the filter membrane in the support 2 forms a gradient, and the particles in different volume gradients receive different centrifugal forces in the centrifugation stage, so that the filtration efficiency in the centrifugation stage can be improved when the side wall of the support 2 is inclined. In a preferred embodiment, the size of the side wall filter hole 41 gradually decreases in the direction from the top of the support 2 to the bottom of the support 2 (not shown in the figure). Generally, when the side wall of the support 2 is inclined, the size of the side wall filter hole 41 gradually decreases in the direction from the top of the support 2 to the bottom of the support 2.

[0045] In the centrifugal device for cells and proteins provided by the embodiment of the utility model, referring to Figure 4 The bottom filter assembly 5 comprises a ring-shaped filter channel 51 and a circular filter channel 52 surrounded by the ring-shaped filter channel 51. A plurality of first partitions 51.2 are arranged in the ring-shaped filter channel 51, and a secondary ring-shaped filter channel 51.1 is formed between adjacent two first partitions 51.2. A plurality of second partitions 52.2 are arranged in the circular filter channel 52, and a secondary circular filter channel 52.1 is formed between adjacent two second partitions 52.2. The above arrangement can ensure that the liquid centrifuged out from the bottom of the support 2 can flow out quickly and the support 2 can be prevented from deforming to the maximum extent in the case of high centrifugation and full sample.

[0046] In some embodiments, the inner diameter of the ring-shaped filter channel 51 is 15-25 mm, preferably 20 mm. In some embodiments, the outer diameter of the ring-shaped filter channel 51 is 25-35 mm, preferably 25 mm. In some embodiments, the number of the first partitions 51.2 is 4-8, preferably 4. In some embodiments, the diameter of the circular filter channel 52 is 10-20 mm, preferably 15 mm. In some embodiments, the number of the second partitions 52.2 is 4-8, preferably 4. In some embodiments, the intervals between adjacent first partitions 51.2 are the same. In some embodiments, the intervals between adjacent second partitions 52.2 are the same. In some embodiments, the width of the first partition 51.2 is 1-3 mm, preferably 2 mm. In some embodiments, the width of the second partition 52.2 is 1-3 mm, preferably 2 mm.

[0047] In the centrifugal device for cells and proteins provided by the embodiment of the utility model, referring to Figures 1-3 andFigure 5 Further comprising an upper anti-collision ring 21 and a lower anti-collision ring 22 arranged around the side wall of the bracket 2, the upper anti-collision ring 21 is close to the top of the bracket 2, and the lower anti-collision ring 22 is close to the bottom of the bracket 2; the outer diameter of the upper anti-collision ring 21 is the same as that of the lower anti-collision ring 22. When the side wall of the bracket 2 is inclined, the bottom of the bracket 2 is far away from the side wall of the centrifugal tube 1, and the center of gravity of the bracket 2 is also deviated to the top of the bracket 2, so that the bottom of the bracket 2 will swing greatly during the centrifugation, which will cause the bracket 2 to collide with the side wall of the centrifugal tube 1 and cause a serious safety accident. Therefore, the outer diameter of the lower anti-collision ring 22 is the same as that of the upper anti-collision ring 21, so that the bracket 2 can maintain a good center of gravity during centrifugation and cannot swing greatly in the centrifugal tube 1, thereby improving the safety during centrifugation.

[0048] In some embodiments, the distance between the upper anti-collision ring 21 and the lower anti-collision ring 22 and the inner wall of the centrifugal tube 1 is 1-3 mm, preferably 2 mm. In some embodiments, the height of the upper anti-collision ring 21 is 3-5 mm, preferably 4 mm. In some embodiments, the height of the lower anti-collision ring 22 is 3-5 mm, preferably 4 mm.

[0049] The above is only the preferred embodiment of the present application, and it should be pointed out that for ordinary skilled in the art, without departing from the technical principles of the present application, a number of improvements and substitutions can be made, and these improvements and substitutions should also be considered as the protection scope of the present application.

Claims

1. A centrifugal device for cells and proteins, characterized by: The centrifugal tube (1), the bracket (2) arranged in the centrifugal tube (1), and the filter membrane slot (3) arranged in the bracket (2) and directed towards the top of the bracket (2), wherein the filter membrane slot (3) is suitable for loading filter membranes; a plurality of sidewall filter assemblies (4) are arranged on the sidewall of the bracket (2) in a circumferential direction of the bracket (2); and a bottom filter assembly (5) is arranged at the bottom of the bracket (2). Each group of the sidewall filter assemblies (4) comprises a plurality of sidewall filter holes (41) arranged in a length direction of the bracket (2).

2. The centrifuge device for cells and proteins according to claim 1, characterized in that: The sidewall of the bracket (2) is gradually inclined inwardly from the top of the bracket (2) to the bottom of the bracket (2), and the inclination angle of the sidewall of the bracket (2) is 30-60°.

3. The centrifuge device for cells and proteins according to claim 2, characterized in that: The size of the sidewall filter hole (41) gradually decreases from the top of the bracket (2) to the bottom of the bracket (2).

4. The centrifuge device for cells and proteins according to any one of claims 1 to 3, characterized in that: The length of the bracket (2) is 80-100 mm; and / or, the thickness of the bracket (2) is 1-3 mm; and / or, the inner diameter of the bracket (2) is 30-35 mm; and / or, the outer diameter of the bracket (2) is 35-40 mm; and / or, the interval angle between two adjacent groups of the sidewall filter assemblies (4) is the same; and / or, the number of the sidewall filter assemblies (4) is 5-10; and / or, the interval between two adjacent sidewall filter holes (41) in the same group of the sidewall filter assemblies (4) is the same; and / or, the number of the sidewall filter holes (41) in each group of the sidewall filter assemblies (4) is 5-10; and / or, the shape of the sidewall filter hole (41) is selected from any one of a circle, an ellipse, or a rectangle.

5. The centrifuge device for cells and proteins according to claim 4, characterized in that: The shape of the sidewall filter hole (41) is a rectangle, the length of the sidewall filter hole (41) is 5-15 mm, the width of the sidewall filter hole (41) is 1-11 mm, and the interval between two adjacent sidewall filter holes (41) in the same group of the sidewall filter assemblies (4) is 3-8 mm.

6. The centrifuge device for cells and proteins according to any one of claims 1 to 3, characterized in that: The bottom filter assembly (5) comprises a ring-shaped filter channel (51) and a circular filter channel (52) surrounded by the ring-shaped filter channel (51). The ring-shaped filter channel (51) is provided with a plurality of first partitions (51.2), and a secondary ring-shaped filter channel (51.1) is formed between two adjacent first partitions (51.2); and the circular filter channel (52) is provided with a plurality of second partitions (52.2), and a secondary circular filter channel (52.1) is formed between two adjacent second partitions (52.2).

7. The centrifuge device for cells and proteins according to claim 6, characterized in that: The inner diameter of the annular filtering channel (51) is 15-25 mm, the outer diameter of the annular filtering channel (51) is 25-35 mm; and / or, the number of the first partitions (51.2) is 4-8; and / or, the diameter of the circular filtering channel (52) is 10-20 mm; and / or, the number of the second partitions (52.2) is 4-8; and / or, the interval between adjacent first partitions (51.2) is the same; and / or, the interval between adjacent second partitions (52.2) is the same; and / or, the width of the first partitions (51.2) is 1-3 mm; and / or, the width of the second partitions (52.2) is 1-3 mm.

8. The centrifuge device for cells and proteins according to any one of claims 1 to 3, characterized in that: Further comprising an upper anti-collision ring (21) and a lower anti-collision ring (22) arranged around the side wall of the support (2), the upper anti-collision ring (21) is close to the top of the support (2), and the lower anti-collision ring (22) is close to the bottom of the support (2); the outer diameters of the upper anti-collision ring (21) and the lower anti-collision ring (22) are equal.

9. The centrifuge device for cells and proteins according to claim 8, characterized in that: The distance between the upper anti-collision ring (21) and the lower anti-collision ring (22) and the inner wall of the centrifugal tube (1) is 1-3 mm.

10. The centrifuge device for cells and proteins according to claim 8, characterized in that: The height of the upper anti-collision ring (21) is 3-5 mm; and / or, the height of the lower anti-collision ring (22) is 3-5 mm.

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