Temperature regulation and preparation device for biological tissue

Abstract

The utility model relates to a biological tissue temperature adjusting and preparing device, and relates to the technical field of laboratory instruments, and the biological tissue temperature adjusting and preparing device comprises a temperature control assembly which forms a cavity used for surrounding an experiment container; the temperature sensor is used for detecting the temperature in the cavity; the Peltier patch is used for adjusting the temperature in the cavity in real time according to a feedback signal of the temperature sensor; and the fan is used for improving the heat dissipation efficiency. The device can be used for heating and refrigerating to maintain the temperature of a biological sample, and can be quickly assembled and disassembled, so that the accuracy and reliability of an experiment are improved, and different experiment requirements are met.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of laboratory instruments, in particular to a temperature regulation and preparation device for biological tissues. BACKGROUND

[0002] In biological experiments, it is often necessary to simulate the body temperature of living organisms or maintain a specific temperature during the preparation of biological samples to facilitate the progress of biological experiments. For example, when preparing a single-cell suspension, operations such as cutting and grinding tissues are performed in experimental containers, and at the same time, the biological samples in each experimental container need to be maintained at the required temperature. Excessive temperature fluctuations can cause denaturation of biological samples and affect experimental results.

[0003] However, the existing preservation devices either cannot be quickly disassembled or can only be heated but not cooled, which leads to an inability to accurately control the temperature of biological samples and thus affects the results of biological experiments. CONTENT OF THE INVENTION

[0004] The purpose of the embodiments of the present application is to provide a temperature regulation and preparation device for biological tissues, which can heat and cool and can be quickly loaded and disassembled to solve the problems existing in the prior art.

[0005] To achieve the above-mentioned purpose, the technical solutions adopted by the embodiments of the present application are as follows:

[0006] In a first aspect, the present application provides a temperature regulation device for biological tissues, comprising:

[0007] a temperature control assembly, which forms a cavity for surrounding an experimental container, and comprises:

[0008] a temperature sensor for detecting the temperature in the cavity;

[0009] a Peltier plate for adjusting the temperature in the cavity in real time according to the feedback signal of the temperature sensor.

[0010] The temperature regulation device for biological tissues provided by the present application has the following advantages. The cavity formed by the temperature control assembly allows the experimental container to be quickly placed or removed, which simplifies the experimental operation, reduces the operation time, improves the experimental efficiency, and also reduces the operation difficulty and labor intensity of the operator. In particular, in experiments that require frequent replacement of samples or containers, the ability to quickly load and disassemble is particularly important. The temperature sensor detects the temperature in the cavity, and the Peltier plate adjusts the temperature in real time according to the feedback signal of the sensor, which can accurately control the temperature in the experimental container, maintain the biological tissue sample at the ideal temperature, prevent the sample from denaturing or losing activity due to temperature fluctuations, and thus improve the accuracy and reliability of the experiment.

[0011] In an optional embodiment, the temperature control assembly further comprises a temperature guide ring, the temperature guide ring forms a cavity for surrounding the experimental container.

[0012] In an optional embodiment, the diameter of the cavity is larger than the diameter of the experimental container, the difference between the diameter of the cavity and the diameter of the experimental container is less than a preset value.

[0013] In an optional embodiment, the Peltier patch is attached to a first surface of the temperature guide ring, the first surface is parallel to the depth direction of the cavity, and the Peltier patch works to heat or cool the temperature guide ring.

[0014] In an optional embodiment, the temperature control assembly further comprises thermal insulation cotton, the thermal insulation cotton circumferentially surrounds a second surface of the temperature guide ring other than the first surface.

[0015] In an optional embodiment, the temperature control assembly further comprises a heat sink, the heat sink is attached to a surface of the Peltier patch away from the temperature guide ring.

[0016] In an optional embodiment, the temperature control assembly further comprises:

[0017] a housing, the housing comprises a first shell and a second shell, the first shell is used to accommodate the temperature control assembly, the first shell is provided with a first opening and a second opening, the first opening is aligned with the heat sink, and the second opening is aligned with the temperature guide ring;

[0018] the second shell is perpendicular to the first shell, the second shell is columnar, and the central axis of the second shell is aligned with the central axis of the cavity.

[0019] In an optional embodiment, the temperature sensor is arranged inside or on the surface of the temperature guide ring.

[0020] In an optional embodiment, the temperature control assembly further comprises a pin, the pin is connected to the Peltier patch, and the pin is used to connect with a control instrument to control the temperature of the Peltier patch.

[0021] In a second aspect, the utility model provides a biological tissue preparation device, including control instrument and any preceding embodiment of biological tissue's temperature regulation device;

[0022] the control instrument comprises an instrument shell;

[0023] a motor, the motor is arranged in the instrument shell, the driving shaft of the motor is used to connect with the experimental container, and the experimental container is driven to rotate, and the biological tissue temperature regulation device is used for temperature control of the experimental container;

[0024] a fan disposed within the instrument housing, an air outlet of the fan being aligned with the temperature control assembly.

[0025] The above embodiment improves the efficiency and quality of biological tissue preparation by integrating temperature control devices and rotary drive equipment, while ensuring the convenience and safety of operation. Moreover, the setting of the fan enhances the heat dissipation effect, especially when the Peltier plate generates heat, the fan can effectively remove the heat and prevent local overheating, thereby protecting the biological tissue from heat damage, and the optimized heat dissipation design provides an efficient, stable and controllable environment for biological tissue preparation.

[0026] The above embodiment provides a biological tissue temperature regulation and preparation device, which forms a cavity around the experimental container through the temperature control assembly. This design allows the temperature control assembly to be quickly installed and removed, as the cavity structure allows the temperature control assembly to easily fit over the experimental container without the need for complex fixing or adjustment processes. The ability to quickly load and unload greatly improves the efficiency of the experiment, especially in experiments that require frequent sample or container changes.

[0027] The temperature sensor detects the temperature in the cavity, and the Peltier plate raises or lowers the temperature in the cavity in real time according to the feedback signal of the sensor to ensure that the biological tissue sample is maintained at the ideal temperature, preventing sample denaturation or activity loss due to temperature fluctuations, thereby improving the accuracy and reliability of the experiment, and adapting to different experimental requirements. BRIEF DESCRIPTION OF DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings needed to be used in the embodiments of the present application. It should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor.

[0029] Figure 1 is a perspective view of the biological tissue temperature regulation device provided by the embodiments of the present application;

[0030] Figure 2 is a perspective view of the temperature control assembly provided by the embodiments of the present application;

[0031] Figure 3 is a schematic view of the experimental container provided by the embodiments of the present application;

[0032] Figure 4 is an exploded schematic view of the temperature control assembly provided by the embodiments of the present application;

[0033] Figure 5 This is a schematic diagram of the biological tissue preparation apparatus provided in the embodiments of this application.

[0034] Icons: 1-Outer shell; 11-First shell; 111-First opening; 112-Second opening; 12-Second shell; 2-Temperature control component; 21-Temperature sensor; 22-Pel patch; 23-Temperature conducting ring; 24-First surface; 25-Second surface; 26-Insulation cotton; 27-Radiator; 28-Pin; 3-Control instrument; 31-Instrument shell; 32-Motor; 33-Fan; 34-Drive shaft; 4-Test tube; 41-Test tube body; 42-Test tube cap; 43-Heated surface. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0036] In the description of this application, it should be noted that the terms "inner" and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product is in use. They are used only for the convenience of describing this application and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0037] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "setup" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0038] Figure 1 This is a perspective view of a temperature regulation device for biological tissue provided in an embodiment of this application. The device includes a housing 1 and a temperature control component 2, with the temperature control component 2 placed inside the housing 1.

[0039] like Figure 2 As shown, the temperature control component 2 includes a temperature sensor 21 and a PAL patch 22.

[0040] The temperature control component 2 forms a cavity surrounding the experimental container, allowing for rapid placement or removal of the container. This rapid loading and unloading capability significantly improves experimental efficiency, especially in experiments requiring frequent sample or container changes. In one embodiment, the cavity can be directly formed using a Pel patch for holding the experimental container.

[0041] At least one temperature sensor 21 is disposed inside or on the surface of the temperature control component 2 to monitor the real-time temperature inside the cavity. The Peltier patch 22 utilizes the Peltier effect to heat or cool in real time based on the feedback signal from the temperature sensor 21, thereby regulating the temperature inside the cavity and realizing temperature feedback and regulation functions. This maintains the temperature inside the cavity within a certain target range, ensuring that the temperature of biological tissues in a specific environment meets the requirements.

[0042] In another embodiment, the temperature control component 2 may include a temperature-conducting ring 23, which forms a cavity for surrounding the experimental container. The inner wall of the temperature-conducting ring 23 is in close contact with the outer surface of the experimental container to achieve heat transfer.

[0043] like Figure 3 As shown, exemplarily, the experimental container for preparing single-cell suspension is a cylindrical test tube 4, which consists of a test tube body 41 and a test tube cap 42. The test tube body 41 has a heated surface 43. The test tube body 41 and the test tube cap 42 are connected together by threads. The temperature-conducting ring 23 transfers heat energy through contact with the heated surface 43. The experimental container can also be of other shapes, as long as the inner wall of the temperature-conducting ring 23 fits tightly against the outer surface of the experimental container.

[0044] In one embodiment, the diameter of the cavity formed by the temperature-conducting ring 23 is larger than the diameter of the experimental container, and the difference between the diameter of the cavity and the diameter of the experimental container is less than a preset value. Specifically, there is a predetermined fitting gap between the temperature-conducting ring 23 and the experimental container. That is, the loading of the experimental container is controlled by the dimensional tolerance between the temperature-conducting ring 23 and the experimental container, forming a small gap fit. This allows the operator to easily install and remove the temperature control device while ensuring heat conduction efficiency.

[0045] like Figure 4 As shown, in one embodiment, the Peltier patch 22 is attached to the first surface 24 of the temperature-conducting ring 23. The first surface 24 is parallel to the depth direction of the cavity. The Peltier patch 22 utilizes the Peltier effect to heat or cool, transferring temperature to the temperature-conducting ring 23, and then to the experimental container inside the cavity, thereby achieving heating or cooling of the experimental container. The temperature-conducting ring 23 is made of a thermally conductive material with good thermal conductivity, such as an aluminum ring, and has a certain thickness.

[0046] In an embodiment, the temperature control assembly 2 further comprises a thermal insulation cotton 26, which circumferentially surrounds the second surface 25 of the temperature guide ring 23 except the first surface 24, effectively isolates the outside temperature from the internal environment of the temperature control assembly 2, and maintains the required temperature stability of the experimental container.

[0047] In an embodiment, the temperature control assembly 2 further comprises a heat sink 27, which is attached to the side surface of the Peltier plate 22 away from the temperature guide ring 23. The heat sink 27 helps to prevent the Peltier plate 22 from overheating due to long-term operation, thereby protecting the Peltier plate 22 from thermal damage. By attaching the heat sink 27, the cooling effect of the Peltier plate 22 can be enhanced, and the cooling efficiency can be improved.

[0048] In an embodiment, the temperature sensor 21 is arranged inside or on the surface of the temperature guide ring 23. The temperature sensor 21 is arranged in the hole of the temperature guide ring 23 to monitor the real-time temperature of the temperature guide ring 23, control the operation of the Peltier plate 22, and maintain the temperature in the temperature guide ring 23 at the target temperature. This design does not require additional space in structure, making the structure more compact, and the monitored temperature more accurate. The temperature sensor 21 can also be arranged on the surface of the temperature guide ring 23, which is not limited here.

[0049] In an embodiment, the temperature control assembly 2 further comprises a pin 28 connected to the Peltier plate 22, which is used to connect with the control instrument 3 to control the operation of the Peltier plate 22, thereby realizing the heating or cooling of the temperature guide ring 23.

[0050] In an embodiment, in combination with Figure 1 、 2 , 4, the temperature control assembly 2 is supported by the shell 1, which provides the necessary stiffness for the temperature control assembly 2, is easy to grip, and to some extent isolates the external air flow, thereby reducing the influence of air flow on the heating temperature.

[0051] The shell 1 comprises a first shell 11 and a second shell 12. The first shell 11 is used to accommodate the temperature control assembly 2, and is provided with a first opening 111 and a second opening 112. The first opening 111 is aligned with the heat sink 27, and the second opening 112 is aligned with the temperature guide ring 23. The second shell 12 is perpendicular to the first shell 11, and is columnar. The central axis of the second shell 12 is aligned with the central axis of the cavity.

[0052] The shell 1 further comprises a window through which the processing state of the biological sample in the experimental container can be observed.

[0053] When the temperature control of the biological tissue sample is needed, the temperature adjusting device is sleeved on the experimental container, the temperature conducting ring 23 of the temperature control assembly 2 is in contact with the outer surface of the experimental container, and temperature conduction is realized. The insertion needle 28 of the temperature control assembly 2 is connected with the external control instrument 3, the control instrument 3 adjusts the working state of the Peltier plate 22 according to the feedback of the temperature sensor 21, so that the temperature of the temperature conducting ring 23 is raised or lowered, so that the temperature is maintained in the required range. The feedback mechanism realizes accurate control of the temperature of the biological tissue sample.

[0054] When the temperature control of the biological tissue sample is not needed, the temperature adjusting device is removed to release the experimental container, and the installation and disassembly are convenient and fast.

[0055] Through the above embodiment, the temperature adjusting device of the utility model can realize accurate temperature control of the biological tissue sample, has the advantages of fast loading and disassembly, and will not affect other operations in the experimental container, and is suitable for various laboratory tissue preparation occasions.

[0056] Figure 5 It is the preparation device for biological tissue provided by the embodiment of the utility model. As shown in the figure, Figure 5 The preparation device for biological tissue includes the control instrument 3 and the temperature adjusting device for biological tissue described in the above embodiment. In an embodiment, the preparation device for biological tissue can include a plurality of temperature adjusting devices for biological tissue.

[0057] In an embodiment, the control instrument 3 includes: an instrument shell 31, a motor 32 and a fan 33.

[0058] The motor 32 is arranged in the instrument shell 31, the driving shaft 34 of the motor 32 is connected with the experimental container, the experimental container is driven to rotate, and the temperature control of the experimental container is carried out by the temperature adjusting device for biological tissue.

[0059] The fan 33 is arranged in the instrument shell 31 and connected with the control instrument 3, and the air outlet of the fan 33 is aligned with the temperature control assembly 2. Specifically, the air inlet of the fan 33 is arranged on the instrument shell 31, and the air outlet is aligned with the radiator 27 of the temperature control assembly 2. By accelerating the air flow, the fan 33 can take away the heat on the surface of the radiator 27 faster, improve the heat management efficiency of the whole temperature control assembly 2, and help to maintain the experimental container at a specific temperature.

[0060] The control instrument 3 controls the temperature adjusting device for biological tissue according to the feedback temperature of the temperature sensor 21, and combines the fan 33 to carry out real-time temperature adjustment on the experimental container, so as to realize accurate temperature control.

[0061] It should be noted that the features in the embodiments of the present application can be combined with each other without conflict.

[0062] The above descriptions are only the preferred embodiments of the present application, and are not intended to limit the present application. The present application can have various changes and modifications for those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A temperature regulating device for biological tissue, characterized by, The device comprises: a temperature control assembly (2) forming a cavity for surrounding an experimental container, the temperature control assembly (2) comprising: a temperature sensor (21) for detecting the temperature in the cavity; a Peltier plate (22) for adjusting the temperature in the cavity in real time according to the feedback signal of the temperature sensor (21).

2. The biological tissue temperature regulating device of claim 1, wherein, The temperature control assembly (2) further comprises a temperature guide ring (23) forming a cavity for surrounding an experimental container.

3. The device for regulating the temperature of biological tissue according to claim 2, characterized in that, The diameter of the cavity is greater than the diameter of the experimental container, and the difference between the diameter of the cavity and the diameter of the experimental container is less than a preset value.

4. The device for regulating the temperature of biological tissue according to claim 2, wherein The Peltier plate (22) is attached to a first surface (24) of the temperature guide ring (23), the first surface (24) being parallel to the depth direction of the cavity, and the Peltier plate (22) is used to heat or cool the temperature guide ring (23).

5. The device for regulating the temperature of biological tissue according to claim 4, characterized in that, The temperature control assembly (2) further comprises thermal insulation cotton (26) circumferentially surrounding a second surface (25) of the temperature guide ring (23) except the first surface (24).

6. The device of claim 4, wherein the temperature regulating means is a cooling device. The temperature control assembly (2) further comprises a heat sink (27) attached to the side surface of the Peltier plate (22) away from the temperature guide ring (23).

7. The device of claim 6, wherein the temperature regulating element is a thermoelectric device. The device further comprises: a housing (1) comprising a first housing (11) and a second housing (12), the first housing (11) being used to accommodate the temperature control assembly (2), the first housing (11) being provided with a first opening (111) and a second opening (112), the first opening (111) being aligned with the heat sink (27), and the second opening (112) being aligned with the temperature guide ring (23); the second housing (12) being perpendicular to the first housing (11), the second housing (12) being columnar, and the central axis of the second housing (12) being aligned with the central axis of the cavity.

8. The device of claim 2, wherein the temperature regulating device is a heat sink. The temperature sensor (21) is arranged inside or on the surface of the temperature guide ring (23).

9. The device for regulating the temperature of biological tissue according to any one of claims 1 to 8, characterised in that, The temperature control assembly (2) further comprises a pin (28) connected to the Peltier plate (22), the pin (28) being used to connect with a control instrument (3) to control the temperature of the Peltier plate (22).

10. An apparatus for preparing a biological tissue, characterized by comprising: The device comprises a control instrument (3) and the biological tissue temperature regulating device of any one of claims 1-9. The control instrument (3) comprises: an instrument housing (31); a motor (32) arranged in the instrument housing (31), a drive shaft (34) of the motor (32) being used to connect with an experimental container to drive the experimental container to rotate, and the biological tissue temperature regulating device being used to control the temperature of the experimental container; a fan (33) arranged in the instrument housing (31), an air outlet of the fan (33) being aligned with the temperature control assembly (2).

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