Culture unit for high-density adherent cells
By optimizing the design of the high-density adherent cell culture unit, the problem of the simple structure of traditional culture units has been solved, achieving efficient cell adhesion and uniform growth, and improving the efficiency and quality of cell culture.
Patent Information
- Application Number
- CN202422737798.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-11
- Publication Date
- 2025-11-07
- Estimated Expiration
- 2034-11-11
AI Technical Summary
Traditional culture units have simple structures and lack optimized designs for the growth characteristics of high-density adherent cells, resulting in low cell adhesion efficiency, uneven growth, and insufficient gas exchange, which affects the growth rate and density of cell culture and reduces the efficiency and success rate of cell culture.
A high-density adherent cell culture unit was designed, which includes a stacked groove structure of internal carrier units, perfusion inlet and outlet tubes and gas exchange channels. Combined with a top cover and locking design, it ensures sealing and stability. The flow guide plate optimizes liquid flow and provides a uniform cell growth environment.
It significantly improved cell adhesion efficiency and growth density, ensured sufficient gas exchange and uniformity of culture medium, enhanced the stability of the culture process and the uniformity of cell growth, and improved the efficiency and quality of cell culture.
Smart Images
Figure CN223522567U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell culture technical field, concretely relates to a kind of culture units of high-density adherent cells. BACKGROUND
[0002] In the field of cell culture, especially for the culture of high-density adherent cells, traditional culture methods often face problems such as low cell adhesion efficiency, uneven cell growth, and insufficient gas exchange. These problems not only affect the growth rate and density of cells, but also can lead to cell death or differentiation during the culture process, thereby reducing the efficiency and success rate of cell culture.
[0003] To overcome these difficulties, the industry has been exploring more efficient and stable cell culture techniques. Among them, the design of the culture unit is a key link. Traditional culture units are usually simple in structure and lack optimization design for the growth characteristics of high-density adherent cells, such as the arrangement of carrier units, gas exchange channels, and the improvement of the internal structure of culture bags. Therefore, developing a culture unit that can better meet the growth needs of high-density adherent cells is of great significance to improve the efficiency and quality of cell culture. SUMMARY
[0004] The purpose of the utility model is to provide a culture unit for high-density adherent cells to solve the problems of traditional culture units being simple in structure and lacking optimization design for the growth characteristics of high-density adherent cells, such as the arrangement of carrier units, gas exchange channels, and the improvement of the internal structure of culture bags.
[0005] To achieve the above purpose, the utility model provides a culture unit for high-density adherent cells, which includes a culture bag. The culture bag has a carrier unit installed in the middle of its interior. The bottom side of the culture bag is connected to a perfusion inlet pipe. The top side of the culture bag is connected to a perfusion outlet pipe. The bottom of the culture bag is connected to a ventilation pipe. The top of the culture bag is connected to an air outlet pipe. The carrier unit includes a number of stacked slots arranged from top to bottom. The side walls of the stacked slots are provided with a number of openings.
[0006] As a preferred embodiment, valves are installed on the perfusion inlet pipe, perfusion outlet pipe, ventilation pipe, and air outlet pipe.
[0007] As a preferred embodiment, a top cover is hinged to the top of the culture bag. Locking plates are installed on the sides of the culture bag and top cover away from the hinge. The locking plates are connected and fixed by locking bolts.
[0008] As a preferred embodiment, a ring of sealing grooves is provided at the bottom of the top cover. A sealing ring is installed on the inner wall of the sealing groove.
[0009] As preferred, the bottom of the carrier unit is fixed in the interior of the culture bag through a support.
[0010] As preferred, the laminated slot is a top-opened circular slot structure, and the bottom outer wall is provided with an arc surface.
[0011] As preferred, the inner wall of the culture bag is provided with flow guide plates at equal intervals from top to bottom.
[0012] As preferred, the flow guide plates are upwardly inclinedly arranged from the inner wall of the culture bag to the outer wall of the carrier unit.
[0013] Compared with the prior art, the high-density adherent cell culture unit has the following beneficial effects:
[0014] In the high-density adherent cell culture unit, the carrier unit installed in the interior of the culture bag has a unique laminated slot structure and a side wall opening design, which significantly improves the adherent efficiency and growth density of cells, so that the cells can grow in a more uniform environment.
[0015] In addition, the top cover design at the top of the culture bag, and the locking plate and locking bolt connection between the top cover and the culture bag, ensure the sealing and stability during the culture process. The sealing groove and sealing ring design at the bottom of the top cover further improve the sealing effect and prevent the entry of external pollutants. The carrier unit is fixed in the interior of the culture bag through a support, which enhances the stability of the structure and makes the cell culture process more reliable.
[0016] Finally, the flow guide plates installed at equal intervals in the inner wall of the culture bag, and the upwardly inclined design of the flow guide plates from the inner wall of the culture bag to the outer wall of the carrier unit, effectively guide the flow of the culture solution, further improving the uniformity of cell growth and the efficiency of gas exchange.
[0017] In summary, the high-density adherent cell culture unit of the present application, through its innovative design and optimization, significantly improves the efficiency and quality of cell culture, and provides strong support for the development of the field of cell culture technology. BRIEF DESCRIPTION OF DRAWINGS
[0018] Fig. 1 is a schematic diagram of the overall structure of the present application;
[0019] Fig. 2 is a schematic diagram of the structure of the carrier unit in the present application;
[0020] Fig. 3 is a schematic diagram of the structure of the top cover in the present application;
[0021] The meanings of the various reference numbers in the drawings are as follows:
[0022] 1, culture bag; 11, perfusion inlet pipe; 12, perfusion outlet pipe; 13, ventilation pipe; 14, gas outlet pipe; 15, top cover; 151, locking bolt; 152, sealing groove; 153, sealing ring; 16, flow guide plate; 2, carrier unit; 21, laminated slot; 22, opening; 3, bracket. DETAILED DESCRIPTION
[0023] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.
[0024] The present application provides a kind of high-density adherent cell culture unit, as shown in figure Figs. 1-3 It comprises culture bag 1, carrier unit 2 is installed in the inside middle of culture bag 1, perfusion inlet pipe 11 is connected to the bottom side of culture bag 1, perfusion outlet pipe 12 is connected to the top side of culture bag 1, ventilation pipe 13 is connected to the bottom of culture bag 1, gas outlet pipe 14 is connected to the top of culture bag 1, carrier unit 2 includes several laminated slots 21 arranged from top to bottom, and several openings 22 are arranged on the side wall of laminated slot 21. The carrier unit 2 installed in the inside middle of culture bag 1 not only provides a stable adhesion and growth platform for cells, but also greatly increases the contact area of cells and culture solution by arranging several laminated slots 21 from top to bottom and openings 22 on the side wall, thereby significantly improving the adhesion efficiency and growth density of cells.
[0025] At the same time, perfusion inlet pipe 11 connected to the bottom of culture bag 1 and perfusion outlet pipe 12 connected to the top of culture bag 1 realize the continuous perfusion and discharge of culture solution, providing sufficient nutrients and growth factors for cells. The ventilation pipe 13 connected to the bottom and the gas outlet pipe 14 connected to the top ensure the smooth exchange of gas in the culture bag, providing the necessary oxygen and carbon dioxide environment for cell growth.
[0026] In this embodiment, valves are installed on perfusion inlet pipe 11, perfusion outlet pipe 12, ventilation pipe 13 and gas outlet pipe 14. This design enables precise control of liquid and gas flow during the culture process. By adjusting the opening and closing degree of the valve, the perfusion speed, discharge volume of the culture solution and the exchange frequency of the gas can be easily adjusted, thereby providing a stable and suitable growth environment for cells.
[0027] Specifically, the top of the culture bag 1 is hinged with a top cover 15, and the culture bag 1 and the top cover 15 are installed with locking plates away from the hinged side, and the locking plates are connected and fixed by locking bolts 151. This design not only facilitates the opening and closing of the culture bag, but also ensures the sealing during the culture process, effectively preventing the entry of external pollutants and ensuring the purity and safety of cell culture.
[0028] Further, the bottom of the top cover 15 is provided with a ring of sealing grooves 152, and the inner wall of the sealing groove 152 is installed with a sealing ring 153. Further enhance the sealing performance of the culture bag. The existence of the sealing ring makes the contact between the top cover and the culture bag more closely, even in the long-term culture process, it can effectively prevent gas leakage and the invasion of external microorganisms.
[0029] Further, the bottom of the carrier unit 2 is fixed inside the culture bag 1 through the support 3. This design enhances the stability and firmness of the carrier unit. During the cell culture process, the carrier unit will not move or shake due to the flow of culture medium or external interference, thereby ensuring that the cells can grow in a stable environment.
[0030] Further, the laminated groove 21 is a circular groove structure with an open top, and the outer wall of the bottom is provided with an arc surface. This design not only facilitates the attachment and growth of cells, but also facilitates the uniform distribution and flow of culture medium. The existence of the arc surface makes the culture medium form a vortex when flowing through the laminated groove, further improving the contact efficiency and material exchange speed of the cells and the culture medium.
[0031] Further, the inner wall of the culture bag 1 is installed with flow guide plates 16 at equal intervals from top to bottom. This design effectively guides the flow direction of the culture medium. The existence of the flow guide plate makes the culture medium flow according to the predetermined path, avoiding the phenomenon of uneven cell growth caused by uneven flow. At the same time, the flow guide plate also plays the role of supporting and fixing the inner wall of the culture bag, enhancing the overall structural strength of the culture bag.
[0032] Further, the flow guide plate 16 is inclined upward from the inner wall of the culture bag 1 to the outer wall of the carrier unit 2. This design further optimizes the flow characteristics of the culture medium. The inclined flow guide plate makes the culture medium form a more uniform flow field when flowing through the carrier unit, thereby improving the contact area and material exchange efficiency of the cells and the culture medium. At the same time, the inclined setting is also beneficial to the rising and discharge of gas, ensuring the smooth exchange of gas in the culture bag. This design further optimizes the flow characteristics of the culture medium. The inclined flow guide plate makes the culture medium form a more uniform flow field when flowing through the carrier unit, thereby improving the contact area and material exchange efficiency of the cells and the culture medium. At the same time, the inclined setting is also beneficial to the rising and discharge of gas, ensuring the smooth exchange of gas in the culture bag.
[0033] The culture unit of high-density adherent cells of the utility model in use, first culture bag 1 inside middle installation carrier unit 2, provides a stable adhesion and growth platform for cell. The lamination slot 21 on the carrier unit 2 and the opening 22 on the side wall thereof increase the contact area of cell and culture solution, promote the adhesion and growth of cell. The top opening and bottom arc surface design of lamination slot 21 facilitate the adhesion of cell, and at the same time, make the culture solution form vortex when flowing, improve the material exchange speed of cell and culture solution.
[0034] By perfusion inlet pipe 11, fresh culture solution is continuously perfused into culture bag 1, and sufficient nutrition and growth factor is provided for cell. The culture solution flows in culture bag 1, passes through lamination slot 21 on carrier unit 2, and carries out sufficient material exchange with cell. The used culture solution is discharged through perfusion outlet pipe 12, realizes the continuous update of culture solution, and maintains the stability and suitability of culture environment.
[0035] Ventilation pipe 13 introduces fresh air or oxygen and other gases into the bottom of culture bag 1, and provides necessary oxygen for cell. The waste gas such as carbon dioxide generated by cell metabolism is discharged through gas outlet pipe 14, and the smooth exchange of gas in culture bag is ensured. The continuous exchange of gas maintains the balance of oxygen and carbon dioxide concentration in culture bag, and provides a good gas environment for cell growth.
[0036] Valves are installed on perfusion inlet pipe 11, perfusion outlet pipe 12, ventilation pipe 13 and gas outlet pipe 14, and the flow of culture solution and gas can be accurately controlled. By adjusting the opening and closing degree of valve, the perfusion speed and discharge amount of culture solution and the exchange frequency of gas can be conveniently adjusted, and a stable and suitable growth environment is provided for cell.
[0037] The top of culture bag 1 is hinged with top cover 15, and fastening connection is realized through locking plate and locking bolt 151, and the sealing property during culture is ensured. The sealing groove 152 at the bottom of top cover 15 and the sealing ring 153 installed on the inner wall thereof further enhance the sealing performance of culture bag, and effectively prevent the entry of external pollutants and the leakage of gas.
[0038] The carrier unit 2 is fixed in the inside of culture bag 1 through support 3, and the stability and firmness of carrier unit are enhanced. The flow guide plate 16 installed on the inner wall of culture bag 1 from top to bottom at equal intervals effectively guides the flow direction of culture solution, and avoids the uneven growth of cell. The inclined setting of flow guide plate 16 further optimizes the flow characteristics of culture solution, and improves the contact area and material exchange efficiency of cell and culture solution.
[0039] The basic principle, main features and advantages of the present application are shown and described above. It should be understood by those skilled in the art that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only preferred examples of the present application and are not intended to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.
Claims
1. A culture unit for high density adherent cells comprising a culture bag (1), characterized in that: The culture bag (1) is internally provided with a carrier unit (2), one side of the bottom of the culture bag (1) is connected with a perfusion inlet pipe (11), one side of the top of the culture bag (1) is connected with a perfusion outlet pipe (12), the bottom of the culture bag (1) is connected with a ventilation pipe (13), the top of the culture bag (1) is connected with an air outlet pipe (14), the carrier unit (2) comprises a plurality of stacked slots (21) arranged from top to bottom, and the side walls of the stacked slots (21) are all provided with a plurality of openings (22).
2. The culture unit for high-density adherent cells according to claim 1, characterized in that: The perfusion inlet pipe (11), the perfusion outlet pipe (12), the ventilation pipe (13) and the air outlet pipe (14) are all provided with valves.
3. The culture unit for high density adherent cells according to claim 1, wherein: The top of the culture bag (1) is hingedly connected with a top cover (15), and the culture bag (1) and the top cover (15) are provided with locking plates on the sides away from the hinged connection; the locking plates are connected and fixed through locking bolts (151).
4. The culture unit for high density adherent cells according to claim 3, characterized in that: The bottom of the top cover (15) is provided with a sealing groove (152), and the inner wall of the sealing groove (152) is provided with a sealing ring (153).
5. The culture unit for high density adherent cells according to claim 1, wherein: The bottom of the carrier unit (2) is fixed in the interior of the culture bag (1) through a support (3).
6. The culture unit for high density adherent cells according to claim 1, wherein: The stacked slot (21) is a circular slot structure with an open top, and the outer wall of the bottom is provided with an arc surface.
7. The culture unit for high density adherent cells according to claim 1, wherein: The inner wall of the culture bag (1) is provided with flow guide plates (16) at equal intervals from top to bottom.
8. The culture unit for high density adherent cells according to claim 7, characterized in that: The flow guide plates (16) are upwardly inclined from the inner wall of the culture bag (1) to the outer wall of the carrier unit (2).