Exosome storage device with dilution function

Abstract

The exosome storage device with the dilution function comprises a storage cylinder, a mixing cylinder and two sets of storage cylinders are installed in the storage cylinder, two sets of liquid inlet pipes and a single set of liquid outlet pipes are fixedly connected to the outer wall of the storage cylinder, a rotating rod is rotatably connected to the interior of the mixing cylinder, and the rotating rod is fixedly connected to the outer wall of the storage cylinder. The outer wall of the rotating rod is rotationally connected with multiple sets of stirring rods, the two sets of storage barrels are internally and fixedly connected with device barrels, the two ends of each device barrel are provided with circulating grooves, and the device barrels are internally and rotationally connected with conveying augers. By means of the conveying auger, the stirring rod and the like, liquid in the storage barrel can be effectively conveyed to the top from the bottom, and circulating flowing of the liquid is achieved. The problem that exosome or normal saline is precipitated due to the action of gravity in the long-time standing process is solved, it is guaranteed that the exosome and the normal saline can be fully mixed when entering the mixing cylinder in cooperation with rotary mixing of the stirring rod, and therefore the uniformity and stability after dilution are remarkably improved.

Description

Technical Field

[0001] This utility model relates to the field of exosome storage technology, and in particular to a storage device for exosomes with dilution function. Background Technology

[0002] Exosomes are small membrane vesicles (30-150 nm) containing complex RNA and proteins. Nowadays, they specifically refer to disc-shaped vesicles with a diameter of 40-100 nm.

[0003] In the prior art, the purified exosomes need to be diluted during the preparation of exosome suspensions. For example, Chinese utility model patent CN219614393U discloses an exosome storage device with dilution function. When in use, this device can independently store and mix physiological saline and exosomes, avoiding contamination during the dilution process. However, during long-term storage, exosomes may gradually precipitate due to gravity or intermolecular interactions, resulting in uneven distribution and mixing during subsequent dilution. Therefore, it is necessary to propose an exosome storage device with dilution function to overcome the shortcomings of the prior art and provide higher mixing uniformity. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a storage device for exosomes with dilution function.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A storage device for exosomes with dilution function includes a storage cylinder, a mixing cylinder and two sets of storage cylinders installed inside the storage cylinder. Two sets of inlet pipes and a single set of outlet pipes are fixedly connected to the outer wall of the storage cylinder. The two sets of inlet pipes are respectively connected to the interior of their adjacent storage cylinders. The outlet pipe is connected to the interior of the mixing cylinder. Both sets of storage cylinders are connected to the interior of the mixing cylinder through infusion pipes, and each infusion pipe is equipped with a solenoid valve. A rotating rod is rotatably connected inside the mixing cylinder. Multiple sets of stirring rods are rotatably connected to the outer wall of the rotating rod. A device cylinder is fixedly connected inside each set of storage cylinders. Flow grooves are opened at both ends of the device cylinder. A conveying auger is rotatably connected inside the device cylinder.

[0007] Preferably, multiple sets of stirring rods are arranged in a ring on the outer wall of the rotating rod, and three rods are arranged at equal intervals on the outer wall of the rotating rod.

[0008] Preferably, a groove is provided on the upper end face of the storage cylinder, and a gear is rotatably connected in the groove, the gear being coaxially and fixedly connected to the rotating rod.

[0009] Preferably, two sets of gears are rotatably connected inside the chute, and their positions correspond to the two sets of conveying screws.

[0010] Preferably, both sets of conveying augers mesh with gears, and the two sets of conveying augers are coaxially and fixedly connected to their adjacent conveying augers.

[0011] Preferably, the gear is coaxially fixedly connected to a throttle, and the storage cylinder is fixedly connected to a handle on its side wall.

[0012] This utility model has the following beneficial effects:

[0013] 1. This invention utilizes a conveying auger and a stirring rod to effectively transport liquid from the bottom to the top of the storage cylinder, achieving liquid circulation. This avoids the problem of sedimentation due to gravity during prolonged standing of exosomes or saline solution. Combined with the rotational mixing of the stirring rod, it ensures thorough mixing of exosomes and saline solution upon entering the mixing cylinder, thereby significantly improving the uniformity and stability after dilution.

[0014] 2. This utility model controls the meshing of gear one and gear two through a throttle, which can simultaneously drive the rotating rod and the conveying auger to rotate synchronously. Users can manually adjust the stirring intensity and speed. This flexible operation method makes the dilution process more precise and controllable. Especially when different levels of stirring intensity or speed are required, users can adjust according to their needs, ensuring precise control of the mixing effect during the dilution of exosomes and solving the problems of inconvenient operation and uncontrollable mixing effect in traditional equipment. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of a storage device for exosomes with dilution function proposed in this utility model;

[0016] Figure 2 for Figure 1 Internal structure diagram.

[0017] Figure 3 for Figure 2 Cross-sectional view of the mixing cylinder and storage cylinder.

[0018] Figure 4 for Figure 3 Cross-sectional view of the storage cylinder.

[0019] In the diagram: 1. Storage cylinder; 2. Handle; 3. Liquid inlet pipe; 4. Slide chute; 5. Gear 1; 6. Gear 2; 7. Rotary handle; 8. Drain pipe; 9. Storage cylinder; 10. Mixing cylinder; 11. Rotary rod; 12. Stirring rod; 13. Device cylinder; 14. Conveying auger. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0021] Reference Figure 1-4 A storage device for exosomes with dilution function includes a storage cylinder 1, a mixing cylinder 10 and two sets of storage cylinders 9 installed inside the storage cylinder 1. Two sets of liquid inlet pipes 3 and a single set of liquid outlet pipes 8 are fixedly connected to the outer wall of the storage cylinder 1. The two sets of liquid inlet pipes 3 are respectively connected to the interior of their adjacent storage cylinders 9. The liquid outlet pipe 8 is connected to the interior of the mixing cylinder 10. Both sets of storage cylinders 9 are connected to the interior of the mixing cylinder 10 through liquid delivery pipes, and each liquid delivery pipe is equipped with a solenoid valve. A rotating rod 11 is rotatably connected inside the mixing cylinder 10. Multiple sets of stirring rods 12 are rotatably connected to the outer wall of the rotating rod 11. A device cylinder 13 is fixedly connected inside each of the two sets of storage cylinders 9. Flow grooves are opened at both ends of the device cylinder 13. A conveying auger 14 is rotatably connected inside the device cylinder 13.

[0022] In practical use, the two sets of storage cylinders 9 respectively store exosomes and physiological saline. With the help of the stirring rod 12 and the conveying auger 14, the solvent entering the mixing cylinder 10 can be stirred to improve the mixing uniformity. With the rotation of the conveying auger 14, the physiological saline and exosomes at the bottom can be transported to the top of the storage cylinder 9 to further improve the uniformity of mixing.

[0023] Multiple sets of stirring rods 12 are distributed in a ring on the outer wall of the rotating rod 11, and three are arranged in a group at equal intervals on the outer wall of the rotating rod 11. A groove 4 is opened on the upper end face of the storage cylinder 1. A gear 5 is rotatably connected in the groove 4. The gear 5 is coaxially fixedly connected to the rotating rod 11. Two sets of gears 6 are rotatably connected in the groove 4, and their positions correspond to the two sets of conveying augers 14. Both sets of conveying augers 14 are meshed with gears 5. The two sets of conveying augers 14 are coaxially fixedly connected to their adjacent conveying augers 14. A handle 7 is coaxially fixedly connected to gear 5. A handle 2 is fixedly connected to the side wall of the storage cylinder 1.

[0024] In this invention, the device is used as follows: by controlling the solenoid valve in the infusion tube, the infusion tubes leading to the exosome solution and physiological saline are opened respectively, allowing the two liquids to enter the mixing cylinder 10. At the same time, the handle 7 is turned, and the gear 5 is rotated. With the meshing of the two sets of gears 6, the rotating rod 11 and the two sets of conveying augers 14 are driven to rotate. The conveying augers 14 can drive the liquid in the storage cylinder 9 from the bottom to the top to ensure the circulation of the liquid in the storage cylinder 9 and to prevent the exosomes or physiological saline from settling due to long-term standing. With the rotation of the rotating rod 11, multiple sets of stirring rods 12 are driven to rotate, which can fully stir the liquid in the mixing cylinder 10, thereby improving the uniformity of mixing.

[0025] Once the required uniformity is achieved, the mixture is discharged from the mixing cylinder 10 through the drain pipe 8 and enters the subsequent use stage.

[0026] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A storage device for exosomes with dilution function, comprising a storage cylinder (1), characterized in that, The storage cylinder (1) is equipped with a mixing cylinder (10) and two sets of storage cylinders (9). The outer wall of the storage cylinder (1) is fixedly connected with two sets of liquid inlet pipes (3) and a single set of liquid outlet pipes (8). The two sets of liquid inlet pipes (3) are respectively connected to the interior of their adjacent storage cylinders (9). The liquid outlet pipe (8) is connected to the interior of the mixing cylinder (10). Both sets of storage cylinders (9) are connected to the interior of the mixing cylinder (10) through liquid delivery pipes, and each liquid delivery pipe is equipped with a solenoid valve. A rotating rod (11) is rotatably connected inside the mixing cylinder (10). Multiple sets of stirring rods (12) are rotatably connected to the outer wall of the rotating rod (11). A device cylinder (13) is fixedly connected inside each of the two sets of storage cylinders (9). Flow grooves are opened at both ends of the device cylinder (13). A conveying auger (14) is rotatably connected inside the device cylinder (13).

2. The exosome storage device with dilution function according to claim 1, characterized in that, Multiple sets of stirring rods (12) are distributed in a ring on the outer wall of the rotating rod (11), and three are arranged at equal intervals on the outer wall of the rotating rod (11).

3. The exosome storage device with dilution function according to claim 2, characterized in that, The storage cylinder (1) has a groove (4) on its upper end face. A gear (5) is rotatably connected in the groove (4). The gear (5) is coaxially fixedly connected to the rotating rod (11).

4. The exosome storage device with dilution function according to claim 3, characterized in that, Two sets of gears (6) are rotatably connected inside the chute (4), and their positions correspond to the two sets of conveying screws (14).

5. A storage device for exosomes with dilution function according to claim 4, characterized in that, Both sets of conveying augers (14) mesh with gear 1 (5), and both sets of conveying augers (14) are coaxially fixedly connected to their adjacent conveying augers (14).

6. A storage device for exosomes with dilution function according to claim 5, characterized in that, The gear (5) is coaxially fixedly connected to a throttle (7), and the storage cylinder (1) is fixedly connected to a handle (2) on its side wall.

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