NSCLC (non-small cell lung cancer) gene targeted capture kit for next-generation sequencing

Abstract

The utility model discloses a non-small cell lung cancer gene targeting capture kit for next-generation sequencing, which comprises a kit body, a kit cover and a support, a plurality of reagent tube fixing components are connected in the kit body through the support, each reagent tube fixing component comprises a positioning cylinder, an air bag ring is fixedly connected in the positioning cylinder, and the air bag ring is fixedly connected with the inner side of the positioning cylinder. The positioning cylinder is used for fixing a tube body of the reagent tube, an inflation valve is arranged outside the positioning cylinder, and the inflation valve penetrates through the positioning cylinder and is communicated to the air bag ring; a base is movably connected to the lower part of the positioning cylinder through a limiting rod and is used for bearing the tube bottom of a reagent tube. According to the utility model, the reagent tubes can be stably clamped, so that the reagent tubes are prevented from loosening, falling off, colliding with one another and the like in the box in the transportation process; meanwhile, the reagent tubes with different thicknesses and lengths can be clamped, and the clamping device is suitable for the reagent tubes with different specifications; in addition, the reagent tube can be effectively prevented from colliding with the wall of the positioning cylinder, and the bearing stability is better.

Description

Technical Field

[0001] This invention relates to the field of biological experimental technology, and in particular to a gene-targeting capture kit for non-small cell lung cancer in next-generation sequencing. Background Technology

[0002] Next-generation sequencing (NGS), also known as high-throughput sequencing, has become one of the most commonly used sequencing technologies due to its high throughput and low cost. It is widely applied in various fields of biology, including the Human Genome Project, transcriptome analysis, and single nucleotide polymorphism analysis.

[0003] Lung cancer is a serious disease that severely threatens human life. Genetic testing can detect EGFR gene mutations and ALK and ROS1 gene fusions, which can facilitate personalized targeted therapy.

[0004] Currently, gene-targeted capture kits for non-small cell lung cancer (NSCLC) in next-generation sequencing contain a variety of reagents (such as unlabeled dNTPs, Taq polymerase, four fluorescently labeled dNTPs, DNA polymerase, and adapter primers), and the dosage specifications vary. Commercially available kits typically place the reagent tubes directly inside the box, or use ordinary brackets or insert the tubes into foam boards for fixation. However, during transportation or handling, collisions or even violent impacts are often unavoidable. This can cause the reagent tubes to easily detach from the brackets or foam boards, resulting in inversion, opening, or breakage. Ultimately, this renders the reagents unusable upon arrival at the sequencing laboratory, affecting the sequencing process. Repurchasing reagents again can delay patient testing, potentially worsening their condition and causing them to miss the optimal treatment window. Utility Model Content

[0005] This invention addresses the technical problems of existing reagent kits having multiple reagent types, different specifications, and unstable placement by providing a gene-targeted capture kit for non-small cell lung cancer in next-generation sequencing.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] This utility model provides a gene-targeted capture kit for non-small cell lung cancer using next-generation sequencing, comprising a box body, a box cover, and a support. Multiple reagent tube fixing components are connected to the box body via the support. Each reagent tube fixing component includes a positioning cylinder, with an air bladder ring fixedly connected inside the positioning cylinder for fixing the body of the reagent tube. An inflation valve is provided outside the positioning cylinder, penetrating the positioning cylinder and connecting to the air bladder ring. A base is movably connected to the bottom of the positioning cylinder via a limiting rod for supporting the bottom of the reagent tube.

[0008] Furthermore, both ends of the bracket are detachably connected to the inner walls of both sides of the box body via limiting supports.

[0009] Furthermore, the inner bottom wall of the limiting support is fixedly connected with a threaded post, and the two sides of the upper surface of the support are formed with inner holes that match the threaded post. After the threaded post is inserted into the inner hole of the support, it is locked by a nut.

[0010] Furthermore, two limiting rods are symmetrically arranged and located on both sides of the positioning cylinder. The top of the limiting rod is fixedly connected to the outer surface of the positioning cylinder, and a limiting ring is sleeved on the outer surface of the limiting rod. The limiting ring is connected to the outer surface of the base through a connecting rod.

[0011] Furthermore, the limiting ring and the limiting rod are fixed together by a positioning handle.

[0012] Furthermore, a protective pad is fixedly connected inside the base, and the protective pad is made of rubber.

[0013] Furthermore, the upper surface of the box is hinged to a lid and locked in place by a latch.

[0014] Furthermore, the latch includes a latch, a fixing seat, and a locking handle. The latch is fixed to the inner edge of the box cover, the fixing seat is fixedly connected to the front of the box body, the fixing seat has a groove for accommodating the latch, and the front side of the fixing seat is threaded with a locking handle. After the latch is inserted into the groove, it is locked in the groove by the locking handle.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0016] 1. This utility model provides a gene-targeted capture kit for non-small cell lung cancer using next-generation sequencing. Through the coordinated arrangement of a support, positioning cylinder, air bladder, and inflation valve, the reagent tube is inserted into the positioning cylinder. Inflation of the air bladder via the inflation valve causes it to expand, stably holding the reagent tube and preventing it from loosening, falling off, or colliding during transport. Furthermore, by controlling the inflation volume, it can hold reagent tubes of different diameters and sizes, making it suitable for various reagent tube specifications. In addition, the air bladder provides cushioning during transport, effectively preventing the reagent tube from bumping against the positioning cylinder wall. When the reagent tube needs to be removed, simply deflate the air bladder to release the fixation, making retrieval easier.

[0017] 2. The present invention provides a gene-targeted capture kit for non-small cell lung cancer in next-generation sequencing. Through the cooperation of the limiting rod, the base and the positioning cylinder, the reagent tube can be placed on the protective pad inside the base when placing the reagent tube, which can support the bottom of the reagent tube and make the reagent tube more stable. Furthermore, the height of the base can be adjusted by the movement of the limiting ring on the limiting rod, so as to place reagent tubes of different lengths, thus meeting the requirements for placing reagent tubes of different types and specifications, and providing better load-bearing stability. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0019] Figure 1 This is an isometric structural diagram of the gene-targeting capture kit for non-small cell lung cancer using next-generation sequencing provided in an embodiment of this utility model.

[0020] Figure 2 This is one of the cross-sectional axonometric structural schematic diagrams of a gene-targeted capture kit for second-generation sequencing of non-small cell lung cancer provided in an embodiment of this utility model.

[0021] Figure 3 This is the second cross-sectional axonometric structural schematic diagram of the gene-targeting capture kit for second-generation sequencing of non-small cell lung cancer provided in this embodiment of the present invention.

[0022] Figure 4 This is one of the magnified structural diagrams of a gene-targeted capture kit for second-generation sequencing of non-small cell lung cancer provided in an embodiment of this utility model.

[0023] Figure 5 The second partially enlarged structural schematic diagram of the gene-targeted capture kit for second-generation sequencing of non-small cell lung cancer provided in this embodiment of the present invention.

[0024] Figure 6 for Figure 1 Enlarged structural diagram at point A in the middle.

[0025] Explanation of reference numerals in the attached figures:

[0026] 1. Box body; 2. Limiting support; 3. Bracket; 4. Positioning cylinder; 5. Airbag ring; 6. Inflation valve; 7. Limiting rod; 8. Limiting ring; 9. Positioning handle; 10. Connecting rod; 11. Base; 12. Protective pad; 13. Threaded post; 14. Nut; 15. Box cover; 16. Fixing seat; 17. Locking handle. Detailed Implementation

[0027] To enable those skilled in the art to better understand the technical solution of this utility model, a further detailed description of this utility model will be provided below in conjunction with the accompanying drawings. The embodiments described below are merely specific examples exemplified in this application to illustrate the technical solution of this application, and are not intended to limit it. The scope of protection of this application is not limited thereto. In the description of this utility model, it should be noted that the terms "inner" and "outer," etc., indicating the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0028] like Figure 1-6 As shown, this utility model provides a gene-targeted capture kit for non-small cell lung cancer using next-generation sequencing, comprising a box body 1, a box cover 15, and a support 3. The box body 1 and the box cover 15 may be made of, for example, plastic.

[0029] The box body 1 is connected to multiple reagent tube fixing components via brackets 3. Each reagent tube fixing component includes a positioning cylinder 4. An air bladder ring 5 is fixedly connected inside the positioning cylinder 4 to fix the body of the reagent tube. An inflation valve 6 is provided on the outside of the positioning cylinder 4. The inflation valve 6 passes through the positioning cylinder 4 and is connected to the air bladder ring 5. A base 11 is movably connected to the bottom of the positioning cylinder 4 via a limiting rod 7 to support the bottom of the reagent tube.

[0030] Regarding the connection between the bracket 3 and the box 1, both ends of the bracket 3 are detachably connected to the inner walls of both sides of the box 1 via limiting supports 2. Specifically, the inner bottom wall of the limiting support 2 is fixedly connected with a threaded post 13, and both sides of the upper surface of the bracket 3 have inner holes matching the threaded post 13. After the threaded post 13 is inserted into the inner hole of the bracket 3, it is locked by a nut 14. Through the use of the threaded post 13 and the nut 14, the bracket 3 can be precisely installed, and the use of the nut 14 for limiting makes the bracket 3 more stable and secure.

[0031] With the setting of limiting support 2, bracket 3, positioning cylinder 4, airbag ring 5 and inflation valve 6, when placing the reagent tube, the reagent tube can be inserted into the positioning cylinder 4, and then air can be inflated into the airbag ring 5 through the inflation valve 6. After the airbag ring 5 is inflated, it expands and can clamp the reagent tube. By controlling the inflation amount, it can meet the clamping needs of reagent tubes of different thicknesses. During transportation, the airbag ring 5 can play a certain buffering role and can effectively prevent the reagent tube from being bumped. When it is necessary to remove the reagent tube, simply deflate the airbag ring 5 to release the fixation of the reagent tube, making it easier to remove.

[0032] Regarding the connection method between the limiting rod 7 and the base 11, specifically, two limiting rods 7 are symmetrically arranged, located on both sides of the positioning cylinder 4. The top of the limiting rod 7 is fixedly connected to the outer surface of the positioning cylinder 4, and a limiting ring 8 is sleeved on the outer surface of the limiting rod 7. The limiting ring 8 is connected to the outer surface of the base 11 through a connecting rod 10. Further, the limiting ring 8 and the limiting rod 7 are fixed together by a positioning handle 9. By setting the limiting ring 8, the limiting rod 7, and the positioning handle 9, when adjusting the height of the base 11, the limiting ring 8 can slide on the limiting rod 7 to change the height of the limiting ring 8, and then the positioning handle 9 is used to fix the position of the limiting ring 8. In a preferred embodiment, in order to improve the cushioning performance, a protective pad 12 is fixedly connected inside the base 11. The protective pad 12 is made of rubber. By setting the protective pad 12, the reagent tube can be supported and protected, reducing wear on the bottom of the reagent tube.

[0033] With the setting of limiting rod 7, limiting ring 8, positioning handle 9, connecting rod 10, base 11 and protective pad 12, when placing reagent tubes, the reagent tubes can be placed on the protective pad 12 inside the base 11, which can support the bottom of the reagent tubes and make the reagent tubes more stable. Furthermore, by moving the limiting ring 8 on the limiting rod 7, the height of the base 11 can be adjusted, so as to place reagent tubes of different lengths, thus meeting the needs of placing different types of reagent tubes and improving applicability.

[0034] Regarding the connection between the box body 1 and the box lid 15, the box lid 15 is hinged to the upper surface of the box body 1 and locked in place by a latch. Specifically, the latch includes a latch tongue, a fixing seat 16, and a locking handle 17. The latch tongue is fixed to the inner edge of the box lid 15, and the fixing seat 16 is fixedly connected to the front of the box body 1. The fixing seat 16 has a groove for accommodating the latch tongue, and the locking handle 17 is threaded to the front side of the fixing seat 16. After the latch tongue is inserted into the groove, it is locked in the groove by the locking handle 17. With the latch tongue, fixing seat 16, and locking handle 17, when the box lid 15 covers the box body 1, tightening the locking handle 17 makes the position of the box lid 15 more secure. Without unlocking, the box lid 15 is not easy to open. During transportation and handling, this method provides better protection for the reagent tubes inside the box.

[0035] Working principle: When in use, the operator can move the limiting ring 8 on the limiting rod 7 according to the required length of the reagent tube, and adjust the height of the base 11. The reagent tube can be inserted into the positioning cylinder 4, with the bottom of the reagent tube inserted into the base 11. Then, air is inflated into the airbag ring 5 through the inflation valve 6. After the airbag ring 5 is inflated, it expands and can clamp the reagent tube. The box cover 15 is flipped over, and then the locking handle 17 can be used to fix the box cover 15, thus completing the placement of the reagent tube.

[0036] In summary, this utility model can stably clamp reagent tubes, preventing them from loosening, falling off, or colliding with each other during transportation; it can also clamp reagent tubes of different thicknesses and lengths, and is suitable for reagent tubes of different specifications; in addition, it can effectively prevent reagent tubes from colliding with the walls of the positioning cylinder, resulting in better load-bearing stability.

[0037] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0038] The embodiments described above are merely specific implementations of this application, used to illustrate the technical solutions of this application, and not to limit them. The protection scope of this application is not limited thereto. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that any person skilled in the art can still modify or easily conceive of changes to the technical solutions described in the foregoing embodiments within the scope of the technology disclosed in this application, or make equivalent substitutions for some of the specific technologies; and these modifications, changes, or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application. All should be covered within the protection scope of this application. Therefore, the protection scope of this application should be determined by the protection scope of the claims.

Claims

1. A gene-targeting capture kit for non-small cell lung cancer using next-generation sequencing, comprising a housing (1), a lid (15), and a support (3), characterized in that, The box (1) is connected to a plurality of reagent tube fixing components by a bracket. Each reagent tube fixing component includes a positioning cylinder (4). An air bag ring (5) is fixedly connected inside the positioning cylinder (4) for fixing the body of the reagent tube. An inflation valve (6) is provided on the outside of the positioning cylinder (4). The inflation valve (6) passes through the positioning cylinder (4) and is connected to the air bag ring (5). A base (11) is movably connected to the bottom of the positioning cylinder (4) by a limiting rod (7) for supporting the bottom of the reagent tube.

2. The gene-targeting capture kit for non-small cell lung cancer using next-generation sequencing according to claim 1, characterized in that, The two ends of the bracket (3) are detachably connected to the inner walls on both sides of the box (1) via limiting supports (2).

3. The gene-targeting capture kit for non-small cell lung cancer using next-generation sequencing according to claim 2, characterized in that, The inner bottom wall of the limiting support (2) is fixedly connected with a threaded column (13). The upper surface of the bracket (3) has inner holes on both sides that match the threaded column (13). After the threaded column (13) is inserted into the inner hole of the bracket (3), it is locked by a nut (14).

4. The gene-targeting capture kit for non-small cell lung cancer using next-generation sequencing according to claim 1, characterized in that, Two limiting rods (7) are symmetrically arranged and located on both sides of the positioning cylinder (4). The top of the limiting rod (7) is fixedly connected to the outer surface of the positioning cylinder (4). A limiting ring (8) is sleeved on the outer surface of the limiting rod (7). The limiting ring (8) is connected to the outer surface of the base (11) through a connecting rod (10).

5. A gene-targeting capture kit for non-small cell lung cancer using next-generation sequencing according to claim 4, characterized in that, The limiting ring (8) and the limiting rod (7) are fixed together by a positioning handle (9).

6. The gene-targeting capture kit for non-small cell lung cancer using next-generation sequencing according to claim 1, characterized in that, The base (11) is internally fixedly connected to a protective pad (12), which is made of rubber.

7. A gene-targeting capture kit for non-small cell lung cancer using next-generation sequencing according to claim 1, characterized in that, The upper surface of the box body (1) is hinged to a lid (15) and locked in place by a latch.

8. A gene-targeting capture kit for non-small cell lung cancer using next-generation sequencing according to claim 7, characterized in that, The latch includes a latch, a fixing seat (16) and a locking handle (17). The latch is fixed to the inner edge of the box cover (15). The fixing seat (16) is fixedly connected to the front of the box body (1). The fixing seat (16) has a groove for accommodating the latch. The front side of the fixing seat (16) is threaded with a locking handle (17). After the latch is inserted into the groove, it is locked in the groove by the locking handle (17).

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