Anti-pollution mixed culture medium cover
By designing a contamination-proof cap for mixed culture media, the problem of contamination during reagent addition was solved, achieving a balance between sealing and permeability, and improving the accuracy of experimental results and the stability of the cell growth environment.
Patent Information
- Application Number
- CN202422617027.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-29
- Publication Date
- 2025-11-11
- Estimated Expiration
- 2034-10-29
AI Technical Summary
Existing culture media are easily contaminated by bacteria in the environment when reagents are added, leading to inaccurate analytical results, and bottle caps are prone to misalignment, causing sample confusion.
A contamination-resistant mixed culture medium cap was designed, comprising a transparent bottle body and a removable cap. The cap contains a permeation membrane assembly, which is controlled to open and close by adjusting a screw and a knob handle. Combined with a return spring and a sealing gasket, it achieves both sealing and air permeability.
It effectively reduces the risk of culture medium contamination, improves the accuracy of experimental results, and maintains gas exchange in the culture environment, which is conducive to cell growth and metabolism.
Smart Images

Figure CN223535086U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of laboratory equipment technology, and more specifically, to a contamination-resistant cap for mixed culture media. Background Technology
[0002] Culture medium is a nutrient substrate composed of different nutrients that is used to support the growth and reproduction of microorganisms, plants, or animals (or tissues).
[0003] Currently, when adding reagents such as antibiotics and buffers to the culture medium in culture flasks to meet the growth and experimental needs of microorganisms, cells, or other organisms, researchers often need to open the flask cap first before adding the required reagents. This process allows bacteria and other microorganisms from the environment to easily enter the flask and contaminate the culture medium each time the cap is opened. Furthermore, after opening multiple flask caps and adding reagents to each one, it is easy to accidentally put the caps back on incorrectly, causing sample confusion and ultimately resulting in analytical results that do not match the actual situation, thus affecting the accuracy of the analytical results.
[0004] In view of this, we propose a contamination-proof cap for mixed culture media. Utility Model Content
[0005] Technical problem to be solved: The purpose of this application is to provide a cap for a mixed culture medium that prevents contamination, thereby solving the technical problem mentioned in the background art.
[0006] Technical solution: The technical solution of this application provides a cap for a mixed culture medium that is designed to prevent contamination, including a transparent bottle body and a detachable cap at the mouth of the transparent bottle body;
[0007] The bottle cap has an opening at the top for adding ingredients. Inside the bottle cap is a membrane assembly. An adjusting screw is threaded onto the side wall of the bottle cap at the location corresponding to the membrane assembly. A knob handle is attached to the end of the adjusting screw.
[0008] The membrane assembly includes a cover frame, a waterproof and breathable membrane connected to the inner cavity of the cover frame, an annular base at the bottom of the cover frame, an annular groove at the top of the annular base, a bottom connecting ring seat slidably connected in the annular groove, and multiple return springs evenly connected to the bottom of the bottom connecting ring seat.
[0009] As an optional solution to the technical solution of this application, the top end of the bottom connecting ring seat extends out from the top opening of the annular groove and is connected to the bottom of the cover frame;
[0010] The end of the return spring away from the bottom connecting ring seat is connected to the bottom wall of the annular groove.
[0011] As an optional solution to the technical solution of this application, one end of the adjusting screw extends into the inner cavity of the bottle cap and is rotatably connected to the side wall of the annular base, while the other end is connected to the knob handle.
[0012] As an optional solution to the technical solution in this application, an annular sealing gasket is connected to the top of the cover frame.
[0013] As an optional solution to the technical solution in this application, a guide side plate is connected to the side wall of the cover frame;
[0014] A horizontal guide groove is horizontally opened on the inner wall of the bottle cap at a position corresponding to the guide side plate, and the end of the guide side plate away from the cap frame is slidably connected to the inside of the horizontal guide groove.
[0015] Beneficial Effects: One or more technical solutions provided in this application have at least the following technical effects or advantages: 1. The addition port opened at the top of the bottle cap not only facilitates the addition of reagents to the culture medium inside the transparent bottle by the experimenter using a pipette, but also effectively reduces the exposed area at the bottle opening, thereby reducing the possibility of contamination of the culture medium. Furthermore, after the reagent is added, the experimenter drives the adjusting screw to rotate by turning the knob handle, and the continuously advancing adjusting screw drives the permeate membrane assembly, allowing the permeate membrane assembly to re-close at the bottom opening of the addition port, preventing bacteria and other microorganisms in the environment from entering the transparent bottle and contaminating the culture medium. This facilitates experimental operations and also helps improve the accuracy of experimental analysis results.
[0016] 2. When the membrane assembly is closed at the bottom opening of the filling port, the annular sealing gasket in the membrane assembly is pressed tightly against the top wall of the inner cavity of the bottle cap under the action of the return spring, ensuring the sealing performance of the membrane assembly.
[0017] 3. After the membrane assembly is placed over the bottom opening of the filling port, bacteria and other microorganisms in the environment are filtered out through the waterproof and breathable membrane. The breathability allows for the exchange of oxygen and carbon dioxide between the inside and outside of the transparent bottle, maintaining the air quality inside the transparent bottle. This is beneficial for the cell growth and metabolism of microorganisms cultured on the culture medium inside the transparent bottle. Attached Figure Description
[0018] Figure 1 This is a schematic diagram of the overall structure of this application.
[0019] Figure 2 This is a schematic diagram of the internal structure of the bottle cap in this application.
[0020] Figure 3 This is a schematic diagram of the permeable membrane assembly in this application.
[0021] The labels in the diagram are as follows: 1. Transparent bottle body; 2. Bottle cap; 3. Filling port; 4. Knob handle; 5. Adjusting screw; 6. Ring base; 7. Return spring; 8. Cap frame; 9. Bottom ring seat; 10. Horizontal guide groove; 11. Waterproof and breathable membrane; 12. Ring sealing gasket; 13. Guide side plate. Detailed Implementation
[0022] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.
[0023] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.
[0024] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or a link; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.
[0025] Reference Figures 1 to 3 This application provides a cap for a mixed culture medium that is designed to prevent contamination. The cap includes a transparent bottle body 1 and a cap 2 that is detachably attached to the mouth of the transparent bottle body 1. The detachable connection methods include threaded connection, snap-fit connection and hinge connection.
[0026] The top of the bottle cap 2 is provided with an adding port 3. The inside of the bottle cap 2 is provided with a membrane permeation assembly. The side wall of the bottle cap 2 is threadedly connected to the membrane permeation assembly. The end of the adjusting screw 5 is connected to a knob handle 4. One end of the adjusting screw 5 extends into the inner cavity of the bottle cap 2 and is rotatably connected to the side wall of the annular base 6, while the other end is connected to the knob handle 4.
[0027] This application utilizes the addition port 3 at the top of the cap 2. This not only facilitates the addition of reagents to the culture medium inside the transparent bottle 1 by the experimenter using a pipette, but also effectively reduces the exposed area at the mouth of the transparent bottle 1, thereby reducing the possibility of contamination of the culture medium. Furthermore, after the reagent is added, the experimenter drives the adjusting screw 5 to rotate by the knob handle 4. The continuously rotating adjusting screw 5 drives the membrane assembly, allowing the membrane assembly to re-close at the bottom opening of the addition port 3. This prevents bacteria and other microorganisms in the environment from entering the transparent bottle 1 and contaminating the culture medium. This facilitates the experimenter's operation and also helps improve the accuracy of the experimental analysis results.
[0028] The membrane assembly includes a cover frame 8, an annular sealing gasket 12 connected to the top of the cover frame 8, a waterproof and breathable membrane 11 connected to the inner cavity of the cover frame 8, an annular base 6 provided below the cover frame 8, an annular groove provided at the top of the annular base 6, a bottom connecting ring seat 9 slidably connected in the annular groove, and the top end of the bottom connecting ring seat 9 extending out from the top opening of the annular groove and connected to the bottom of the cover frame 8.
[0029] Multiple return springs 7 are evenly connected to the bottom of the bottom connecting ring seat 9, and the end of the return spring 7 away from the bottom connecting ring seat 9 is connected to the bottom wall of the annular groove.
[0030] A guide side plate 13 is connected to the side wall of the cover frame 8;
[0031] A horizontal guide groove 10, adapted to the guide side plate 13, is horizontally formed on the inner wall of the bottle cap 2 at a position corresponding to the guide side plate 13. The end of the guide side plate 13 away from the cap frame 8 is slidably connected inside the horizontal guide groove 10. The guiding effect of the horizontal guide groove 10 on the movement of the guide side plate 13 improves the stability of the permeable membrane assembly movement when driven by rotating the adjusting screw 5 within the inner cavity of the bottle cap 2.
[0032] When the membrane assembly is closed at the bottom opening of the filling port 3, the annular sealing gasket 12 in the membrane assembly is pressed tightly against the top wall of the inner cavity of the bottle cap 2 under the elastic force of the return spring 7, ensuring the sealing performance of the membrane assembly.
[0033] After the membrane assembly is placed over the bottom opening of the filling port 3, bacteria and other microorganisms in the environment are filtered out by the waterproof and breathable membrane 11. The breathability allows for the exchange of oxygen and carbon dioxide inside and outside the transparent bottle 1, maintaining the air quality inside the transparent bottle 1. This is beneficial for the cell growth and metabolism of microorganisms cultured on the culture medium inside the transparent bottle 1.
[0034] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.
Claims
1. A cap for a contamination-resistant mixed culture medium, characterized in that: Includes a transparent bottle body (1), and the bottle body (1) has a detachable cap (2) at the bottle mouth; The bottle cap (2) has an opening (3) at the top and a membrane assembly inside. An adjusting screw (5) is threadedly connected to the side wall of the bottle cap (2) at the position corresponding to the membrane assembly. A knob handle (4) is connected to the end of the adjusting screw (5). The permeable membrane assembly includes a cover frame (8), a waterproof and breathable membrane (11) is connected in the inner cavity of the cover frame (8), an annular base (6) is provided below the cover frame (8), an annular groove is provided on the top of the annular base (6), a bottom connecting ring seat (9) is slidably connected in the annular groove, and multiple return springs (7) are evenly connected to the bottom of the bottom connecting ring seat (9).
2. The anti-contamination mixed culture medium cap according to claim 1, characterized in that: The top end of the bottom connecting ring seat (9) extends out from the top opening of the annular groove and is connected to the bottom of the cover frame (8); The end of the return spring (7) away from the bottom connecting ring seat (9) is connected to the bottom wall of the annular groove.
3. The anti-contamination mixed culture medium cap according to claim 1, characterized in that: One end of the adjusting screw (5) extends into the inner cavity of the bottle cap (2) and is rotatably connected to the side wall of the annular base (6), while the other end is connected to the knob handle (4).
4. The anti-contamination mixed culture medium cap according to claim 1, characterized in that: The top of the cover frame (8) is connected to an annular sealing gasket (12).
5. The anti-contamination mixed culture medium cap according to claim 1, characterized in that: The cover frame (8) has a guide side plate (13) connected to its side wall; A horizontal guide groove (10) is horizontally opened on the inner wall of the bottle cap (2) at a position corresponding to the guide side plate (13), and the end of the guide side plate (13) away from the cap frame (8) is slidably connected to the inside of the horizontal guide groove (10).