Stacked filter membrane structure extraction device for stem cell exosome preparation

Abstract

The utility model discloses a stack type filter membrane structure extraction device for preparing stem cell exosomes, which relates to the technical field of extraction devices and comprises an outer tube body, a bearing tube seat is fixed on the outer side of the upper end of the outer tube body, a closed tube cover is sleeved on the outer side of the bearing tube seat, and four buckle seats are fixed at the lower end of the closed tube cover in an array manner. Four T-shaped sliding grooves are formed in the outer side of the closed pipe cover in an array mode, a sliding block is connected to the inner side of each T-shaped sliding groove in a sliding mode, a T-shaped sliding seat is fixed to the outer side of each sliding block, and an elastic belt is fixed to the position, located on the outer side of the closed pipe cover, between every two T-shaped sliding seats. According to the stack type filter membrane structure extraction device for stem cell exosome preparation, through the overall structure matching design, rapid installation between the bearing tube base and the closed tube cover is achieved, connection between the closed tube cover and the bearing tube base is firmer through the four downward-sliding T-shaped sliding bases and the elastic bands, and the extraction effect is better. And meanwhile, subsequent disassembly is facilitated.

Description

Technical Field

[0001] This utility model relates to the field of extraction device technology, and in particular to a stacked filter membrane structure extraction device for preparing stem cell exosomes. Background Technology

[0002] Exosomes are tiny membrane vesicles containing complex nucleic acids and proteins with a diameter of 30nm-150nm. They can be used for early detection, diagnosis and prevention of diseases. The extraction method of exosomes is usually ultracentrifugation, which is achieved by pouring exosomes into centrifuge tubes equipped with filter membranes for filtration and separation. However, it is not easy to disassemble and clean the centrifuge tubes and filter membranes later, resulting in reduced extraction efficiency. At the same time, the purity of extraction by filtration with a single filter membrane is not high.

[0003] For example, Chinese utility model patent (CN213652474U) discloses an exosome extraction device, which describes: "It includes an outer tube mechanism, an inner tube mechanism is snapped into the inner side of the outer tube mechanism, a first extraction mechanism is snapped into the inner side of the inner tube mechanism, and a second extraction mechanism is snapped into the inner side of the first extraction mechanism."

[0004] The above-mentioned extraction device achieves rapid cleaning and replacement of the filter screen through a nested inner tube structure. However, the inner and outer tubes are snap-fitted together, which can easily cause them to jam as the centrifugal speed increases, making disassembly and separation difficult.

[0005] For example, Chinese utility model patent (CN221566135U) discloses an exosome extraction device, which states: "By setting a stacked filter membrane structure, not only is the separation and extraction function of exosomes realized, but it is also easy to install and disassemble, and improves the efficiency of subsequent cleaning."

[0006] The above-mentioned extraction device improves the extraction purity through a stacked filter membrane structure. The setting of the first gasket and the second gasket solves the problem of easy jamming between the inner and outer tubes. However, the threaded connection between the upper and lower tube caps is also prone to jamming due to tightening or separation due to loosening as the centrifugation speed increases. Therefore, this application proposes a stacked filter membrane structure extraction device for the preparation of stem cell exosomes. Utility Model Content

[0007] Based on this, it is necessary to provide a stacked filter membrane structure extraction device for stem cell exosome preparation to address the above-mentioned technical problems. Through the overall structural design, the device enables rapid installation between the support tube and the sealing tube cap. After installation, the four sliding T-shaped slides and elastic bands make the connection between the sealing tube cap and the support tube more secure, while facilitating subsequent disassembly, thereby making it easier to clean or replace the first and second filter membranes.

[0008] To solve the above-mentioned technical problems, the present invention adopts the following technical solution:

[0009] A stacked filter membrane structure extraction device for the preparation of stem cell exosomes, which is applied to the preparation of stem cell exosomes;

[0010] The device includes an outer tube body, a support tube seat fixed to the outer side of the upper end of the outer tube body, a filter membrane assembly disposed inside the outer side of the outer tube body, a sealing tube cover sleeved on the outer side of the support tube seat, four snap fasteners fixed in an array at the lower end of the sealing tube cover, the upper ends of the four snap fasteners being in contact with the bottom end of the support tube seat, four T-shaped sliding grooves arrayed on the outer side of the sealing tube cover, a slider slidably connected to the inner side of each T-shaped sliding groove, a T-shaped sliding seat fixed to the outer side of each slider, and an elastic band fixed between every two T-shaped sliding seats and on the outer side of the sealing tube cover.

[0011] As a preferred embodiment of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model, the filter membrane assembly includes a first guide post. Four first guide posts are arrayed and fixed at the upper end of the outer tube body and inside the support tube seat. A first inner tube body is slidably connected between the four first guide posts and inside the outer tube body. A first filter membrane body is fixed at the lower end of the first inner tube body and inside the outer tube body. Four second guide posts are arrayed and fixed at the upper end of the first inner tube body and inside the support tube seat. A second inner tube body is slidably connected between the four second guide posts and inside the first inner tube body. A second filter membrane body is fixed at the lower end of the second inner tube body and inside the first inner tube body.

[0012] In a preferred embodiment of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model, a first ring pad is sleeved on the outside of each first guide post, the upper end of the first ring pad is attached to the first inner tube body, and the lower end of the first ring pad is attached to the outer tube body. A second ring pad is sleeved on the outside of each second guide post, the upper end of the second ring pad is attached to the second inner tube body, and the lower end of the second ring pad is attached to the first inner tube body.

[0013] As a preferred embodiment of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model, each of the T-shaped slides is provided with anti-slip stripes on its outer side.

[0014] As a preferred embodiment of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model, each of the T-shaped slides has a snap-fit ​​head attached to the inner side of its upper end.

[0015] As a preferred embodiment of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model, a cross buckle frame is fixed at the upper end between the four buckle heads, and the cross buckle frame is located outside the closed tube cap.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The stacked filter membrane structure extraction device for stem cell exosome preparation provided by this utility model enables rapid installation between the support tube seat and the closed tube cap through the overall structural design. After installation, the connection between the closed tube cap and the support tube seat is more secure by the four sliding T-shaped slides and elastic bands, which also facilitates subsequent disassembly, thereby facilitating the cleaning or replacement of the first and second filter membrane bodies.

[0018] The outer tube, the first inner tube, and the second inner tube are stacked together by the guidance of the first and second guide pillars and the compression of the closed tube cap. The first and second ring pads prevent the outer tube, the first inner tube, and the second inner tube from getting stuck, thus effectively improving the efficiency of multiple extractions of stem cell exosomes. Attached Figure Description

[0019] To more clearly illustrate the solutions in this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0020] Figure 1 A schematic diagram of the overall structure of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model;

[0021] Figure 2 A schematic diagram of the filter membrane assembly of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model;

[0022] Figure 3 A schematic diagram of the structure between the outer tube, the first inner tube, and the second inner tube of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model.

[0023] Figure 4 A schematic diagram of the structure between the support tube seat and the sealing tube cap of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model;

[0024] Figure 5 A schematic diagram of the upper end of the sealed tube cap of the stacked filter membrane structure extraction device for preparing stem cell exosomes provided by this utility model.

[0025] The markings in the diagram are explained as follows:

[0026] 1. Outer tube body; 2. First inner tube body; 3. Second inner tube body; 4. First filter membrane body; 5. Second filter membrane body; 6. Support tube seat; 7. First guide post; 8. First ring gasket; 9. Second guide post; 10. Second ring gasket; 11. Sealing tube cap; 12. T-shaped slide groove; 13. Buckle seat; 14. Slider; 15. T-shaped slide seat; 16. Elastic band; 17. Anti-slip stripes; 18. Cross buckle bracket; 19. Buckle head. Detailed Implementation

[0027] As described in the background art, the above-mentioned extraction device solves the problem of easy jamming between the inner and outer tubes by setting the first gasket and the second gasket. However, the threaded connection between the upper tube cap and the lower tube cap is also prone to jamming due to tightening or separation due to loosening as the centrifugal speed increases.

[0028] To solve this technical problem, this utility model provides a stacked filter membrane structure extraction device for the preparation of stem cell exosomes, which is applied to the preparation of stem cell exosomes;

[0029] The device includes an outer tube body 1, a support tube seat 6 fixed to the outer side of the upper end of the outer tube body 1, a filter membrane assembly disposed inside the outer tube body 1, a sealing tube cover 11 sleeved on the outer side of the support tube seat 6, four snap fasteners 13 fixed in an array at the lower end of the sealing tube cover 11, the upper ends of the four snap fasteners 13 being in contact with the bottom end of the support tube seat 6, four T-shaped sliding grooves 12 being arrayed on the outer side of the sealing tube cover 11, a slider 14 being slidably connected to the inner side of each T-shaped sliding groove 12, a T-shaped sliding seat 15 being fixed to the outer side of each slider 14, and an elastic band 16 being fixed between every two T-shaped sliding seats 15 and located on the outer side of the sealing tube cover 11.

[0030] The stacked filter membrane structure extraction device for stem cell exosome preparation provided by this utility model enables rapid installation between the support tube seat 6 and the sealing tube cap 11 through the overall structural design. After installation, the connection between the sealing tube cap 11 and the support tube seat 6 is made more secure by the four sliding T-shaped slides 15 and the elastic band 16, which also facilitates subsequent disassembly, thus making it easier to clean or replace the filter membrane assembly.

[0031] It should be noted that, unless otherwise specified, the embodiments and features and technical solutions in the present invention can be combined with each other.

[0032] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0033] Example 1:

[0034] Please refer to Figure 1-4 A stacked filter membrane structure extraction device for stem cell exosome preparation includes an outer tube 1, a support tube seat 6 fixed on the outer side of the upper end of the outer tube 1, and a filter membrane assembly disposed on the inner side of the outer tube 1. Specifically, the filter membrane assembly includes first guide posts 7. Four first guide posts 7 are arrayed and fixed on the upper end of the outer tube 1 and inside the support tube seat 6. A first inner tube 2 is slidably connected between the four first guide posts 7 and inside the outer tube 1. A first filter membrane 4 is fixed on the lower end of the first inner tube 2 and inside the outer tube 1. To avoid direct contact between the first inner tube 2 and the upper end of the outer tube 1, which may cause subsequent jamming, a first ring pad 8 is sleeved on the outer side of each first guide post 7. The upper end of the first ring pad 8 is attached to the first inner tube 2, and the lower end of the first ring pad 8 is attached to the outer tube 1.

[0035] Four second guide posts 9 are fixedly arranged in an array at the upper end of the first inner tube 2 and inside the bearing tube seat 6. A second inner tube 3 is slidably connected between the four second guide posts 9 and inside the first inner tube 2. A second filter membrane 5 is fixed at the lower end of the second inner tube 3 and inside the first inner tube 2. To avoid direct contact between the second inner tube 3 and the upper end of the first inner tube 2, which could easily cause jamming, a second ring pad 10 is sleeved on the outside of each second guide post 9. The upper end of the second ring pad 10 is in contact with the second inner tube 3, and the lower end of the second ring pad 10 is in contact with the first inner tube 2.

[0036] In order to seal the upper end of the bearing tube seat 6, a sealing tube cover 11 is fitted on the outside of the bearing tube seat 6. In order to facilitate the quick installation between the sealing tube cover 11 and the bearing tube seat 6, four snap fasteners 13 are fixed in an array at the lower end of the sealing tube cover 11, and the upper ends of the four snap fasteners 13 are all in contact with the bottom end of the bearing tube seat 6.

[0037] To further enhance the secure connection between the buckle seat 13 and the bearing tube seat 6, four T-shaped grooves 12 are arrayed on the outer side of the closed tube cover 11. A slider 14 is slidably connected to the inner side of each T-shaped groove 12. A T-shaped slide seat 15 is fixed to the outer side of each slider 14. An elastic band 16 is fixed between every two T-shaped slide seats 15 and on the outer side of the closed tube cover 11. To facilitate the sliding of the T-shaped slide seats 15, anti-slip stripes 17 are provided on the outer side of each T-shaped slide seat 15.

[0038] Example 2:

[0039] The stacked filter membrane structure extraction device for preparing stem cell exosomes provided in Example 1 has been further optimized, specifically, as follows: Figure 5As shown, in order to facilitate the simultaneous sliding of four T-shaped slide blocks 15, so that the four elastic bands 16 can move quickly to the bottom of the bearing tube seat 6, a buckle head 19 is snapped on the inner side of the upper end of each T-shaped slide block 15, and a cross buckle frame 18 is fixed at the upper end between the four buckle heads 19. The cross buckle frame 18 is located outside the closed tube cover 11.

[0040] The stacked filter membrane structure extraction device for stem cell exosome preparation provided by this utility model is used as follows: During installation, the first guide post 7 and the second guide post 9 are used to guide the first inner tube 2 to be sleeved inside the outer tube 1 and the second inner tube 3 to be sleeved inside the first inner tube 2, respectively. The first ring pad 8 and the second ring pad 10 are used to ensure that the upper ends of the outer tube 1, the first inner tube 2 and the second inner tube 3 do not stick together, thereby avoiding jamming. The stacked structure of the first filter membrane 4 and the second filter membrane 5 is used to improve the extraction purity.

[0041] Next, the sealing cap 11 is fitted onto the outside of the bearing tube seat 6, so that all four snap fasteners 13 are in contact with the bottom end of the bearing tube seat 6, completing the snap-fit ​​connection between the sealing cap 11 and the bearing tube seat 6. At this time, guided by the sliding guide of the slider 14 and the T-shaped slide groove 12, the four T-shaped slide seats 15 slide down simultaneously, so that the four elastic bands 16 move down synchronously with the T-shaped slide seats 15. Figure 1 As shown, this prevents the four elastic bands 16 from contacting the closed tube cap 11 and makes them fit against the bottom of the bearing tube seat 6. This limits the position of the four elastic bands 16 while using their elasticity to bind the four buckle seats 13, making it difficult for the four buckle seats 13 to flip outward. This improves the connection between the closed tube cap 11 and the bearing tube seat 6 and completes the quick installation.

[0042] During disassembly, it is only necessary to flip out the four elastic bands 16 and slide the four T-shaped slides 15 upwards, and then flip up the four buckle seats 13 so that the buckle seats 13 no longer contact the bottom of the bearing tube seat 6, thereby facilitating the removal of the sealing tube cover 11, and then disassembling the filter membrane assembly. The overall disassembly and assembly are convenient and effectively improve the extraction efficiency.

Claims

1. A stacked filter membrane structure extraction device for preparing stem cell exosomes, characterized in that, The device includes an outer tube body (1), a support tube seat (6) is fixed on the outer side of the upper end of the outer tube body (1), a filter membrane assembly is provided on the inner side of the outer tube body (1), a closed tube cover (11) is sleeved on the outer side of the support tube seat (6), four buckle seats (13) are fixed in an array at the lower end of the closed tube cover (11), the upper ends of the four buckle seats (13) are all in contact with the bottom end of the support tube seat (6), four T-shaped sliding grooves (12) are arranged in an array on the outer side of the closed tube cover (11), a slider (14) is slidably connected to the inner side of each T-shaped sliding groove (12), a T-shaped sliding seat (15) is fixed on the outer side of each slider (14), and an elastic band (16) is fixed between every two T-shaped sliding seats (15) and on the outer side of the closed tube cover (11).

2. The stacked filter membrane structure extraction device for preparing stem cell exosomes according to claim 1, characterized in that, The filter membrane assembly includes a first guide post (7), four first guide posts (7) are fixedly arranged in an array at the upper end of the outer tube (1) and inside the support tube seat (6), a first inner tube (2) is slidably connected between the four first guide posts (7) and inside the outer tube (1), a first filter membrane (4) is fixedly arranged at the lower end of the first inner tube (2) and inside the outer tube (1), four second guide posts (9) are fixedly arranged in an array at the upper end of the first inner tube (2) and inside the support tube seat (6), a second inner tube (3) is slidably connected between the four second guide posts (9) and inside the first inner tube (2), and a second filter membrane (5) is fixedly arranged at the lower end of the second inner tube (3) and inside the first inner tube (2).

3. The stacked filter membrane structure extraction device for preparing stem cell exosomes according to claim 2, characterized in that, Each of the first guide posts (7) is fitted with a first ring gasket (8) on its outer side. The upper end of the first ring gasket (8) is in contact with the first inner tube (2), and the lower end of the first ring gasket (8) is in contact with the outer tube (1). Each of the second guide posts (9) is fitted with a second ring gasket (10) on its outer side. The upper end of the second ring gasket (10) is in contact with the second inner tube (3), and the lower end of the second ring gasket (10) is in contact with the first inner tube (2).

4. The stacked filter membrane structure extraction device for preparing stem cell exosomes according to claim 1, characterized in that, Each of the T-shaped slides (15) has anti-slip stripes (17) on its outer side.

5. The stacked filter membrane structure extraction device for preparing stem cell exosomes according to claim 1, characterized in that, Each of the T-shaped slides (15) has a snap-fit ​​head (19) attached to the inner side of its upper end.

6. The stacked filter membrane structure extraction device for preparing stem cell exosomes according to claim 5, characterized in that, A cross buckle bracket (18) is fixed at the upper end between the four buckle heads (19), and the cross buckle bracket (18) is located outside the closed tube cover (11).

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