Centrifugal extraction device for cytoplasm grains

Abstract

The utility model relates to the technical field of cell centrifugal extraction, in particular to a cytoplasm grain centrifugal extraction device which comprises a centrifugal machine body, a cross-shaped loading frame is rotatably installed in the centrifugal machine body, three groups of loading rings are installed on the cross-shaped loading frame in an array mode, loading holes are formed in the loading rings in an array mode, and the loading holes are communicated with the loading frame. First mounting grooves are formed in the cross-shaped loading frame in an array mode, first mounting blocks are fixedly mounted on the left side and the right side of the loading circular ring, and the first mounting blocks are inserted into the first mounting grooves. According to the centrifugal machine, more loading choices are provided under the condition that the weight of the cross-shaped loading frame is not greatly increased through the loading rings capable of carrying out selective loading, so that materials can be placed at a more proper position for centrifugation, the adaptability of the centrifugal machine during use is improved, and the centrifugal machine is convenient to use. In addition, a probe is arranged to prompt whether a balancing pipe is loaded at the corresponding position of the loaded hole or not.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell centrifugal extraction technical field, concretely is a kind of cell plasmid centrifugal extraction device. BACKGROUND

[0002] Centrifuge is to make the density different cells in solution realize separation, concentration or purification under the action of centrifugal force using centrifugal sedimentation principle. Among them, centrifugal force is one of important parameters of centrifuge, generally use rotation speed to describe a centrifuge, such as low-speed centrifuge, high-speed centrifuge etc., the rotation speed of existing centrifuge is one of important standards to measure centrifugal effect, but the centrifugal effect of centrifuge to material actually also depends on the distance of material and centrifugal center, in addition, the rotation speed of centrifuge is also influenced by the weight of loading rack of centrifuge, the loading rack of existing centrifuge is limited by weight so it cannot be made too large, so the above loading hole cannot be too far from center point, so the position selection of loading is not enough, in addition, the centrifuge of existing laboratory needs to be trimmed during use, but trimming can be forgotten during use, which can damage centrifuge, therefore we propose a kind of cell plasmid centrifugal extraction device to solve the above problems. SUMMARY

[0003] The utility model aims at providing a kind of cell plasmid centrifugal extraction device to solve the problems of less selection when loading material of existing centrifuge and easy to forget trimming during use proposed in the above background.

[0004] To achieve the above object, the utility model provides the following technical scheme: a kind of cell plasmid centrifugal extraction device, including centrifuge body, cross loading rack is rotatably installed in the centrifuge body, three groups of loading annular are installed in array on the cross loading rack, loading hole is opened in array on the loading annular, first installation slot is opened in array on the cross loading rack, first installation block is fixedly installed on the left and right sides of the loading annular, the first installation block is inserted in first installation slot, fixed cover is installed on the upside of the cross loading rack, fixed screw is installed in the center position of the fixed cover, screw sleeve is installed in the center position of the cross loading rack, the fixed screw is installed in screw sleeve by thread.

[0005] Preferably, the first mounting groove is fixedly provided with a fixed rod, the fixed rod is provided with an inserting slot, the lower side of the inserting slot is provided with a first clamping slot, a rotating inserting drum is rotatably arranged in the first mounting block, a clamping block is fixedly arranged in the inserting drum, the width of the inserting drum is matched with the inserting slot, the height of the inserting drum is matched with the first clamping slot, the inserting drum is clamped in the first clamping slot, the upper end of the inserting drum is provided with a first clamping slot, the upper end of the first mounting block is provided with a second clamping slot, the size of the first clamping slot is matched with the second clamping slot, and a clamping block is movably arranged in the second clamping slot.

[0006] Preferably, the upper side of the first mounting block is provided with a second mounting groove, the upper end of the inserting drum is provided with a torsion spring, one end of the torsion spring is fixedly arranged on the inserting drum, and the other end of the torsion spring is fixedly arranged on the inner wall of the second mounting groove.

[0007] Preferably, the upper side of the second mounting groove is provided with a second clamping slot, the distance between the upper end of the inserting drum and the upper side of the second clamping slot is matched with the height of the clamping block, and a handle is fixedly arranged on the upper side of the clamping block.

[0008] Preferably, the diameters of the three groups of loading rings are different, and the loading rings have the same center.

[0009] Preferably, the second mounting block is fixedly arranged at the four corners of the upper end of the cross-shaped loading frame, a limiting rod is fixedly arranged on the second mounting block, four groups of limiting slots are arrayed on the fixed cover, and the limiting rod is inserted into the limiting slot.

[0010] Preferably, the fixed cover is hollow, and probes are movably arranged in the fixed cover, the positions of the probes are opposite to the positions of the loading holes, and a transparent plate is fixedly arranged on the upper side of the fixed cover.

[0011] Preferably, the upper side of the probe is provided with a color developing block, and the color developing blocks with the same diameter have the same color.

[0012] Compared with the prior art, the utility model has the advantages that: the loading rings with selective loading are used to make the weight of the cross-shaped loading frame not greatly increase, and more loading options are provided, so that the materials can be placed in more suitable positions for centrifugation, the adaptability of the equipment in use is improved, and the probes are arranged to prompt whether the corresponding positions of the loaded holes have the loading balance pipes, and the stability of the equipment in use is improved. BRIEF DESCRIPTION OF DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the description of the embodiments or the prior art. Obviously, the drawings described below are only some of the embodiments of the present application, and not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0014] Figure 1 It is a structure front view schematic diagram of the present application.

[0015] Figure 2 It is a structure separation schematic diagram of the fixed cover of the present application.

[0016] Figure 3 It is a structure separation schematic diagram of the loading ring of the present application.

[0017] Figure 4 It is a structure cross section schematic diagram of the loading ring of the present application.

[0018] Figure 5 It is a structure schematic diagram of the clamping block of the present application.

[0019] Figure 6 It is a structure schematic diagram of the present application Figure 4 It is a local enlarged schematic diagram of A of the present application.

[0020] In the figure: 1, centrifuge body; 2, cross loading frame; 3, loading ring; 4, loading hole; 5, first installation groove; 6, first installation block; 7, fixed cover; 8, fixed screw; 9, screw sleeve; 10, fixed rod; 11, insertion slot; 12, first clamping groove; 13, insertion rotating drum; 14, clamping block; 15, second installation groove; 16, torsional spring; 17, first clamping groove; 18, second clamping groove; 19, clamping block; 20, second clamping groove; 21, handle; 22, second installation block; 23, limiting rod; 24, limiting groove; 25, probe; 26, transparent plate; 27, color development block. DETAILED DESCRIPTION

[0021] The technical solutions in the embodiments of the present application will be described clearly and completely below in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, and not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0022] Please refer to Figures 1-6The utility model provides a kind of embodiment: a cytoplasm plasmid centrifugal extraction device, including centrifuge body 1, cross loading frame 2 is rotatably installed in centrifuge body 1, three groups of loading annular ring 3 are arrayed installed on cross loading frame 2, loading hole 4 is arrayed in on loading annular ring 3, first installation groove 5 is arrayed in on cross loading frame 2, first installation block 6 is fixedly installed on the left and right sides of loading annular ring 3, first installation block 6 is inserted in first installation groove 5, fixed cover 7 is installed on the upside of cross loading frame 2, fixed screw rod 8 is installed in the center position of fixed cover 7, screw sleeve 9 is installed in the center position of cross loading frame 2, fixed screw rod 8 is installed in screw sleeve 9 by thread, the selective loading of loading annular ring 3 can be selected in the equipment, additionally still be provided with probe 25 to prompt the corresponding position of the hole that has been loaded whether there is loading balance tube, increase the stability when equipment is used.

[0023] Further, fixed rod 10 is fixedly installed at first installation groove 5, insertion slot 11 is opened in fixed rod 10, first clamping groove 12 is opened in the lower side of insertion slot 11, insertion rotating drum 13 is rotatably installed in first installation block 6, clamping block 14 is fixedly installed in insertion rotating drum 13, the width of insertion rotating drum 13 is compatible with insertion slot 11, the height of insertion rotating drum 13 is compatible with first clamping groove 12, insertion rotating drum 13 is clamped in first clamping groove 12, first clamping groove 17 is opened in the upper end of insertion rotating drum 13, second clamping groove 18 is opened in the upper end of first installation block 6, the size of first clamping groove 17 and second clamping groove 18 is compatible, clamping block 19 is movably inserted in second clamping groove 18, the size of clamping block 19 and second clamping groove 18 is compatible, the structure makes that loading annular ring 3 can be locked between fixed rod 10 and insertion rotating drum 13 after loading, and this locking is not affected by the rotation of cross loading frame 2, increase the stability when equipment is used.

[0024] Further, second installation groove 15 is opened in the upside of first installation block 6, torsional spring 16 is arranged on the upper end of insertion rotating drum 13, one end of torsional spring 16 is fixedly installed on insertion rotating drum 13, the other end of torsional spring 16 is fixedly installed on the inner wall of second installation groove 15, the structure makes that clamping block 14 is always clamped in first clamping groove 12 under the action of torsional spring 16, further increase the stability when equipment is used.

[0025] Further, second clamping groove 20 is opened in the upside of second installation groove 15, the spacing between the upper end of insertion rotating drum 13 and the upside of second clamping groove 20 is compatible with the height of clamping block 19, handle 21 is fixedly installed on the upside of clamping block 19, the structure makes that clamping block 19 not only can release the locking of loading annular ring 3, but also can be lifted loading annular ring 3 by clamping when releasing the locking, increase the convenience when equipment is used.

[0026] Further, the three groups of loading rings 3 have different diameters but the same center, and the loading rings 3 are not in contact with each other, which makes the centrifugal force on the material on the loading rings 3 easier to calculate, and the non-contact is to prevent the extrusion caused by the deformation of the loading rings 3 during rotation.

[0027] Further, the second mounting block 22 is fixedly installed at the four corners of the upper end of the cross loading frame 2, the limiting rod 23 is fixedly installed on the second mounting block 22, and the limiting slot 24 is arrayed on the fixed cover 7, and the limiting rod 23 is inserted into the limiting slot 24, which makes the fixed cover 7 more stable on the cross loading frame 2, and increases the stability of the equipment during use.

[0028] Further, the fixed cover 7 is hollow, and the probe 25 is movably inserted in the fixed cover 7, the position of the probe 25 is opposite to the position of the loading hole 4, and the transparent plate 26 is fixedly installed on the upper side of the fixed cover 7, which makes the probe 25 detect the material loading and makes the upper side adhere to the transparent plate 26, and increases the convenience of checking whether it is balanced.

[0029] Further, the upper side of the probe 25 is provided with the color developing block 27, and the colors of the color developing blocks 27 at the same diameter are the same, which further increases the convenience of balancing check.

[0030] Working principle: when in use, the first mounting block 6 is placed in the first mounting slot 5, then the clamping block 19 is inserted into the first clamping slot 17 and rotated, so that the clamping block 14 is aligned with the insertion slot 11, so that the fixed rod 10 can be inserted into the insertion rotating cylinder 13, then the clamping block 19 is taken out, at this time the torsional spring 16 rebounds to make the clamping block 14 clamped in the first clamping slot 12 to realize fixation, and the same principle is used when disassembling, after the clamping block 19 is rotated, it will abut against the second clamping slot 20, so that it can be lifted to make the loading ring 3 be lifted, then the fixed cover 7 is placed on the cross loading frame 2, so that the limiting rod 23 is inserted into the limiting slot 24, then the fixed screw 8 is screwed into the screw sleeve 9 to fix it, at this time if there is material on a group of probes 25, the material will be lifted to make the color developing block 27 adhere to the transparent plate 26, at this time the user can clearly observe whether the corresponding position of the probe 25 is lifted, if not, it means that it is not balanced, the surface of the transparent plate 26 is frosted, so only when the color developing block 27 adheres to the transparent plate 26 can the color be clearly observed, and the above is the whole working principle of the utility model.

[0031] It is apparent for a person skilled in the art that the present application is not restricted to the details of the above exemplary embodiments, but that it can be implemented in other concrete forms without departing from the spirit or the essential characteristics of the present application. Therefore, the embodiments should be considered as exemplary only, and not limiting, the scope of the present application being defined by the appended claims rather than by the above description, and all changes coming within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference signs in the claims should not be construed as limiting the claims concerned.

Claims

1. A cytoplasmic plasmid centrifugal extraction device, comprising a centrifuge body (1), a cross loading frame (2) is rotatably installed in the centrifuge body (1), characterized in that: Three groups of loading annular rings (3) are arrayed and installed on the cross loading frame (2), loading holes (4) are arrayed and formed on the loading annular rings (3), first installation grooves (5) are arrayed and formed on the cross loading frame (2), first installation blocks (6) are fixedly installed on the left and right sides of the loading annular rings (3), the first installation blocks (6) are inserted into the first installation grooves (5), a fixed cover (7) is installed on the upper side of the cross loading frame (2), a fixed screw rod (8) is installed at the center position of the fixed cover (7), a screw sleeve (9) is installed at the center position of the cross loading frame (2), and the fixed screw rod (8) is threadedly installed in the screw sleeve (9).

2. The cytoplasmic plasmid centrifugal extraction device according to claim 1, characterized in that: A fixed rod (10) is fixedly installed at the first installation groove (5), an insertion groove (11) is formed on the fixed rod (10), a first clamping groove (12) is formed on the lower side of the insertion groove (11), an insertion rotating cylinder (13) is rotatably installed in the first installation block (6), a clamping block (14) is fixedly installed in the insertion rotating cylinder (13), the width of the insertion rotating cylinder (13) is matched with the insertion groove (11), the height of the insertion rotating cylinder (13) is matched with the first clamping groove (12), the insertion rotating cylinder (13) is clamped in the first clamping groove (12), a first clamping groove (17) is formed on the upper end of the insertion rotating cylinder (13), a second clamping groove (18) is formed on the upper end of the first installation block (6), the size of the first clamping groove (17) is matched with the second clamping groove (18), a clamping block (19) is movably inserted into the second clamping groove (18), and the size of the clamping block (19) is matched with the second clamping groove (18).

3. A cytoplasmic plasmid centrifugal extraction device according to claim 2, characterized in that: A second installation groove (15) is formed on the upper side of the first installation block (6), a torsional spring (16) is arranged on the upper end of the insertion rotating cylinder (13), one end of the torsional spring (16) is fixedly installed on the insertion rotating cylinder (13), and the other end of the torsional spring (16) is fixedly installed on the inner wall of the second installation groove (15).

4. A cytoplasmic plasmid centrifugal extraction device according to claim 3, wherein: A second clamping groove (20) is formed on the upper side of the second installation groove (15), the spacing between the upper end of the insertion rotating cylinder (13) and the upper side of the second clamping groove (20) is matched with the height of the clamping block (19), and a handle (21) is fixedly installed on the upper side of the clamping block (19).

5. The cytoplasmic plasmid centrifugal extraction device according to claim 1, wherein: The diameters of the three groups of loading annular rings (3) are different, and the centers of the loading annular rings (3) are the same.

6. The cytoplasmic plasmid centrifugal extraction device according to claim 1, wherein: Second installation blocks (22) are fixedly installed at the four corners of the upper end of the cross loading frame (2), limiting rods (23) are fixedly installed on the second installation blocks (22), four groups of limiting grooves (24) are arrayed and formed on the fixed cover (7), and the limiting rods (23) are inserted into the limiting grooves (24).

7. The cytoplasmic plasmid centrifugal extraction device according to claim 1, wherein: The fixed cover (7) is hollow, and probes (25) are movably inserted in the fixed cover (7) in an array, the position of the probes (25) is opposite to the position of the loading holes (4), and a transparent plate (26) is fixedly installed on the upper side of the fixed cover (7).

8. A cytoplasma plasmid centrifugal extraction device according to claim 7, characterized in that: The upper side of the probe (25) is provided with a color developing block (27), and the color developing blocks (27) at the same diameter are of the same color.

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