Gel mold for agarose gel electrophoresis
By using modular components and magnetic strips for magnetic connection, the problem of loose comb plates in traditional agarose gel electrophoresis molds is solved, thus achieving stability of the sample channel and accuracy of electrophoresis experiments, and improving experimental efficiency and reliability of results.
Patent Information
- Application Number
- CN202423054659.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-11
- Publication Date
- 2025-11-18
- Estimated Expiration
- 2034-12-11
AI Technical Summary
Traditional agarose gel electrophoresis molds lack an effective comb plate fixation mechanism, which leads to comb plate loosening, affects the consistency of sample channel spacing, and results in inaccurate and unreliable electrophoresis results.
Modular components such as mold box, end cap, comb plate, mounting column, fastener, and card holder are used, combined with magnetic strip and insertion seat magnetic connection method to ensure stable installation of comb plate and spacing adjustment.
It improves the flexibility and efficiency of experiments, ensures the uniformity of sample channels and the accuracy of electrophoresis experiments, prevents gel leakage, and improves the reliability of experimental results.
Smart Images
Figure CN223558867U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to agarose gel electrophoresis technical field, especially relate to a gel mould of agarose gel electrophoresis. BACKGROUND
[0002] Agarose gel electrophoresis is an experimental technique widely used in molecular biology, biochemistry and other fields, mainly used for separating, identifying and purifying biological macromolecules such as DNA, RNA, etc. When performing agarose gel electrophoresis experiments, agarose gel needs to be prepared first. This step usually involves dissolving agarose powder in a buffer solution, heating and dissolving it, and then pouring it into a specific mold to cool and solidify. Therefore, the design and performance of the gel mold are crucial to the quality of the gel and the success of the electrophoresis experiment.
[0003] Traditional agarose gel electrophoresis gel molds, although generally use simple mold and end cap structure, and built-in a comb plate, due to the traditional device in design often lack of effective fixation mechanism to the comb plate, leading to the comb plate after installation easy to loosen, the looseness of the comb plate will first directly affect its pre-adjusted spacing. In agarose gel electrophoresis experiments, the teeth grooves on the comb plate form sample channels in the gel. The spacing between these channels is crucial to the layout of the finished multi-channel agarose gel. They determine the distribution and separation effect of the sample in the gel. Once the comb plate is loose, these spacings may change, resulting in uneven width of the sample channels, and even possible overlap or misalignment between channels, which will seriously interfere with the migration and separation of DNA fragments during electrophoresis, making the experimental results inaccurate and unreliable. SUMMARY
[0004] The utility model discloses a gel mould of agarose gel electrophoresis to solve the technical problems in the prior art.
[0005] To achieve the above object, the utility model adopts the following technical scheme: a gel mould of agarose gel electrophoresis, comprising a mold box, and end caps installed at both ends of the mold box, a comb plate is provided on the mold box, an installation column connected with the comb plate is provided on the mold box, installation grooves are respectively formed at both ends of the installation column, fixed parts are respectively arranged on the inner sides of the two installation grooves, and clamping seats matched with the mold box are respectively arranged at one end of the two fixed parts; a magnetic strip is arranged on the installation column, and an insertion seat magnetically attracted to the magnetic strip is arranged on one side of the comb plate close to the installation column.
[0006] By adopting the technical scheme, the device is composed of modular components such as a mold box, end covers, comb plates, mounting columns, fixing members, and card seats, so that the entire device is easy to assemble and disassemble, and experimenters can quickly build or disassemble the device as needed, improving the flexibility and efficiency of the experiment, and conveniently adjusting the spacing between the comb plates.
[0007] Optionally, the mold box is rectangular, and two long edges of the mold box are respectively provided with edge seats in the same horizontal plane as the upper end surface of the mold box.
[0008] By adopting the technical scheme, the rectangular design of the mold box facilitates the operation and use of experimenters, while ensuring uniform solidification of the gel. The design of the edge seat not only increases the strength and stability of the mold box, but also provides a flat surface for connecting the comb plate, so that the comb plate can be more stably mounted on the mold box.
[0009] Optionally, the surfaces of the two edge seats are respectively provided with scale tables.
[0010] By adopting the technical scheme, the design of the scale table enables experimenters to accurately adjust the spacing between multiple comb plates, thereby ensuring the consistency and accuracy of the experiment.
[0011] Optionally, two sides of the two end covers are respectively provided with butt strips, the outer sides of the two pairs of butt strips and the lower ends of the pair of end covers are respectively provided with sealing pads, and the two long edges of the mold box are respectively provided with butt grooves for mounting the pair of butt strips.
[0012] By adopting the technical scheme, the butt strips cooperate with the butt grooves on the mold box to ensure that the end covers can tightly close the two ends of the mold box, and the design of the sealing pads further enhances the sealing between the end covers and the mold box, preventing leakage of the glue solution during preparation.
[0013] Optionally, the side surface of the mounting column is provided with an insertion slot, the insertion slot is adapted to the insertion of the insertion seat, and the magnetic strip is mounted on the inner side of the insertion slot.
[0014] By adopting the technical scheme, the design of the insertion slot provides space for the insertion of the insertion seat of the comb plate, ensuring accurate alignment and magnetic attraction between the magnetic strip and the insertion seat, which improves the accuracy and stability of the installation of the comb plate.
[0015] Optionally, the fixing member comprises a connecting column mounted on the inner side of the mounting slot and connected with the clamping seat at one end, and a limiting seat connected with the end of the mounting column at two ends respectively, the surface of the limiting seat is provided with a through hole for the connecting column to pass through, and the connecting column and the limiting seat are provided with a reset spring therebetween.
[0016] By using the above technical scheme, the clamping seat is connected with the inner side of the mounting slot through the connecting column, the stability of the comb plate on the mounting column is ensured, this fixing mode can prevent the comb plate from moving or tilting due to external force during the experiment, thereby ensuring the accuracy and consistency of the gel preparation.
[0017] Optionally, the fixing member comprises a locking screw mounted on the end of the clamping seat.
[0018] By using the above technical scheme, the locking screw is used as the fixing member, the fixing is increased in multiple choices, the operation is simple, and the fastening connection between the comb plate and the mold box can be realized only by rotating the screw, this fixing mode does not need complex installation steps or tools, and the efficiency of the experiment operation is greatly improved.
[0019] Optionally, the clamping seat is in the shape of a hook, and the side of the clamping seat that is attached to the side of the edge seat is provided with an anti-skid pad.
[0020] By using the above technical scheme, the hook-shaped design of the clamping seat facilitates the experimenters to quickly install and dismount the comb plate, and the stability of the comb plate on the mold box is ensured, and the anti-skid pad further enhances the friction between the clamping seat and the edge seat, and prevents the comb plate from sliding or falling off during the experiment.
[0021] Compared with the prior art, the beneficial effects of the utility model are:
[0022] 1. By introducing the mounting column, the mounting slot, the fixing member, and the magnetic attraction connection mode of the magnetic force strip and the insertion seat, the utility model realizes the effective fixation of the comb plate, this design not only prevents the comb plate from loosening after installation, but also ensures that the previously adjusted distance between the comb plates remains unchanged during the gel preparation, thereby ensuring the uniform width of the sample channel, avoiding the overlapping or misalignment between the channels, and improving the accuracy and reliability of the electrophoresis experiment.
[0023] 2. The fixing member of the utility model is designed flexibly, which can realize automatic clamping through the reset spring and the connecting column, and can be manually fixed through the locking screw, this design enables the experimenters to conveniently adjust and fix the comb plate according to the actual needs, and improves the convenience and flexibility of the operation.
[0024] 3. By installing the sealing gasket under the butt joint strip and the end cap end part, and opening the butt joint groove on the side wall of the mold box, the gel mold realizes the tight sealing of the two ends of the mold box, and the tight sealing design can effectively prevent the gel liquid inside the mold box from leaking during the gel preparation and curing process. BRIEF DESCRIPTION OF DRAWINGS
[0025] Figure 1 It is a top view stereoscopic structure schematic diagram of the gel mold of the utility model;
[0026] Figure 2 It is a sectional view of the comb plate and the mounting column of the utility model;
[0027] Figure 3 It is a sectional view of the fixed part and the card seat of the utility model in embodiment one;
[0028] Figure 4 It is a stereoscopic structure schematic diagram of the end cap of the utility model;
[0029] Figure 5 It is a sectional view of the fixed part and the card seat of the utility model in embodiment two.
[0030] In the drawing: 1, mold box; 101, along the seat; 102, scale table; 103, butt joint groove; 2, end cap; 201, butt joint strip; 202, sealing gasket; 203, handle; 3, comb plate; 4, mounting column; 401, plug-in groove; 5, mounting groove; 6, fixed part; 61, connecting column; 62, limiting seat; 63, perforation; 64, reset spring; 7, card seat; 701, anti-skid pad; 8, magnetic strip; 9, insertion seat; 10, locking bolt. DETAILED DESCRIPTION
[0031] The technical scheme of the utility model will be described clearly and completely below in combination with the drawings in the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.
[0032] In the description of the utility model, it should be explained that the terms "intermediate", "upper", "lower", "left", "right", "inner", "outer" and the like indicate the orientation or position relationship shown in the drawings, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, therefore it cannot be understood as a limitation on the utility model.
[0033] As Figure 1—4 as shown, the specific scheme of the embodiment is as follows: a gel mold for agarose gel electrophoresis, comprising a mold box 1, the mold box 1 is rectangular, the mold box 1 is the basic structure of the agarose gel electrophoresis, the mold box 1 provides the space required for gel preparation, when making agarose gel, the raw materials of the prepared agarose gel are put into the mold box 1 after heating, and then the mold box 1 is solidified, the rectangular design is convenient for experimental operation and uniform solidification of the gel, two long sides of the mold box 1 are respectively provided with a side seat 101, the side seat 101 installed on the long side of the mold box 1 not only increases the strength and stability of the mold box 1, but also keeps the same horizontal plane with the upper end face of the mold box 1, which provides a flat support surface for the installation of the comb plate 3, and the two side seats 101 are in the same horizontal plane with the upper end face of the mold box 1;
[0034] The surfaces of the two side seats 101 are respectively provided with a scale table 102, the scale table 102 is installed on the side seat 101, which is convenient for the experimenter to accurately measure and adjust the distance between the brushes, so as to ensure the consistency and accuracy of the experiment, and the end caps 2 installed at both ends of the mold box 1, the end caps 2 close the two ends of the mold box 1, prevent the gel from being polluted or interfered by the outside during preparation, at the same time, the handle 203 on the end cap 2 is designed to facilitate the experimenter to open and close the end cap 2.
[0035] Two sides of the two end caps 2 are respectively provided with a butt joint strip 201, the outer sides of the two pairs of butt joint strips 201 and the lower ends of a pair of end caps 2 are respectively provided with a sealing gasket 202, the two long side walls of the mold box 1 are respectively provided with a butt joint groove 103 for installing a pair of butt joint strips 201, the surface of the end cap 2 is fixedly connected with a handle 203, the butt joint strip 201 is installed on the side of the end cap 2, cooperates with the butt joint groove 103 of the mold box 1, realizes the butt joint of the two ends of the mold box 1, and the sealing gasket 202 enhances the sealing property of the butt joint, prevents the gel from seeping out, and ensures the cleanliness of the experimental environment and the integrity of the gel.
[0036] The mold box 1 is provided with a comb plate 3, the tooth groove on the comb plate 3 can form clear separation channels during gel preparation, when the gel is solidified in the mold box 1, these channels will serve as the channels of the samples in the electrophoresis experiment, ensuring that each sample migrates independently in the electric field, avoiding cross contamination between samples;
[0037] The mold box 1 is provided with a mounting column 4 connected with the comb plate 3, the mounting column 4 provides a basis for the installation and fixation of the comb plate 3, the design enables the comb plate 3 to be stably installed on the mold box 1, and facilitates the adjustment of the distance between the comb plates 3, two ends of the mounting column 4 are respectively provided with a mounting groove 5, and the inner sides of the two mounting grooves 5 are respectively provided with a fixing piece 6;
[0038] The fixing member 6 comprises a connecting column 61 mounted on the inner side of the pair of mounting grooves 5 and connected with one end of the clamping seat 7, and a limiting seat 62 connected with the end of the pair of mounting columns 4, respectively, the surface of the pair of limiting seats 62 is respectively provided with a through hole 63 for the pair of connecting columns 61 to pass through, and the pair of connecting columns 61 and the pair of limiting seats 62 are respectively provided with a reset spring 64, the fixing member 6 realizes firm fixing of the comb plate 3 through cooperation of the connecting column 61 and the limiting seat 62 and elastic force of the reset spring 64, which not only ensures stability of the comb plate 3 in use, but also facilitates the experimenter to adjust the position of the comb plate 3 according to needs.
[0039] One end of the two fixing members 6 is respectively provided with a clamping seat 7 matched with the mold box 1, the pair of clamping seats 7 are respectively hook-shaped, the clamping seat 7 is matched with the mold box 1, which further enhances the stability of the comb plate 3, and the hook-shaped design facilitates the experimenter to quickly install and dismount the comb plate 3. One side of the pair of clamping seats 7 matched with the side of the edge seat 101 is respectively provided with an anti-skid pad 701, and the anti-skid pad 701 ensures close matching between the clamping seat 7 and the edge seat 101, preventing the comb plate 3 from loosening in use.
[0040] The mounting column 4 is provided with a magnetic strip 8, one side of the comb plate 3 close to the mounting column 4 is provided with an insertion seat 9 magnetically attracted to the magnetic strip 8, the side of the mounting column 4 is provided with an insertion slot 401, the insertion slot 401 can be used for butt joint insertion of the insertion seat 9, and the magnetic strip 8 is mounted on the inner side of the insertion slot 401, the magnetic attraction connection mode of the magnetic strip 8 and the insertion seat 9 simplifies the installation process of the comb plate 3, improves the convenience and accuracy of installation, and at the same time, the connection mode also enhances the stability of the comb plate 3 on the mounting column 4; the insertion slot 401 provides a space for butt joint insertion of the insertion seat 9, ensuring accurate alignment and magnetic attraction connection between the magnetic strip 8 and the insertion seat 9.
[0041] In the second embodiment, as shown in the figure, Figure 5 The mounting column 4 is integrally formed with the pair of clamping seats 7, the clamping seat 7 is provided with the fixing member 6, the fixing member 6 comprises a locking bolt 10 mounted on the end of the clamping seat 7, and the locking bolt 10 is located in the groove of the clamping seat 7, which simplifies the structure of the mold box 1, improves the integrity and stability of the mold box 1, and at the same time, also facilitates the experimenter to quickly install and dismount the comb plate 3, and the locking bolt 10 provides more firm fixing as another fixing mode.
[0042] The experimental steps of the above embodiments are as follows:
[0043] Before preparing the agarose gel, the weighed agarose powder is added into a certain amount of electrophoresis buffer, heated in a microwave oven or a boiling water bath until completely dissolved, and a dye is added: when the agarose solution is cooled to an appropriate temperature (e.g., 50-60℃), ethidium bromide (or other fluorescent dyes) is added to a final concentration of 0.5 μg / ml, and mixed thoroughly;
[0044] The mold box 1 is placed on a water platform, and then the cooled agarose solution is slowly poured into the mold box 1 to avoid air bubbles;
[0045] The comb plate 3 is installed, the distance between the comb plates 3 is adjusted according to the experimental requirements, and the comb plate 3 is firmly fixed on the mold box 1 through the installation column 4 and the fixing piece 6;
[0046] The gel solution is allowed to completely solidify at room temperature, and after the gel is completely solidified, the comb plate 3 is removed, and whether the surface is flat, bubble-free and crack-free is checked.
[0047] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.
Claims
1. An agarose gel electrophoresis gel mold comprising a mold box and end caps mounted on both ends of the mold box, and a comb plate provided on the mold box, characterized in that, The mold box is provided with a mounting column connected with the comb plate, two ends of the mounting column are respectively provided with mounting grooves, inner sides of the two mounting grooves are respectively provided with fixing members, one end of the two fixing members is respectively provided with a clamping seat matched with the mold box; the mounting column is provided with a magnetic strip, one side of the comb plate close to the mounting column is provided with an insertion seat magnetically attracted with the magnetic strip.
2. A gel mold for agarose gel electrophoresis according to claim 1, characterized in that: The mold box is rectangular, two long edges of the mold box are respectively provided with edge seats, and the two edge seats are on the same horizontal plane with the upper end surface of the mold box.
3. A gel mold for agarose gel electrophoresis according to claim 2, characterized in that: The surfaces of the two edge seats are respectively provided with scale tables.
4. A gel mold for agarose gel electrophoresis according to claim 1, characterized by: Two sides of the two end covers are respectively provided with butt strips, outer sides of the two butt strips and lower ends of a pair of the end covers are respectively provided with sealing pads, and two long edge side walls of the mold box are respectively provided with butt grooves for mounting a pair of the butt strips.
5. A gel mold for agarose gel electrophoresis according to claim 1, characterized by: The side surface of the mounting column is provided with an insertion groove, the insertion groove is used for butt insertion of the insertion seat, and the magnetic strip is mounted on the inner side of the insertion groove.
6. A gel mold for agarose gel electrophoresis according to claim 1, characterized by: The fixing member includes a connecting column mounted on the inner side of a pair of the mounting grooves and connected with the clamping seat at one end, and a limiting seat connected with the end of a pair of the mounting columns, the surface of a pair of the limiting seats is respectively provided with a through hole for a pair of the connecting columns to pass through, and a reset spring is arranged between a pair of the connecting columns and a pair of the limiting seats.
7. A gel mold for agarose gel electrophoresis according to claim 1, characterized by: The fixing member includes a locking bolt mounted on the end of the clamping seat.
8. A gel mold for agarose gel electrophoresis according to claim 2, characterized by: A pair of the clamping seats are respectively in the shape of hooks, and one side of the clamping seat matched with the side edge of a pair of the edge seats is respectively provided with an anti-skid pad.