Liquid injection assembly and polypeptide synthesis equipment
The automated control of the liquid injection component solves the problems of high cost and low accuracy of manual operation in traditional peptide synthesis, achieving efficient and accurate liquid injection and improving the automation level and synthesis effect of peptide synthesis equipment.
Patent Information
- Application Number
- CN202423033871.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-09
- Publication Date
- 2025-11-21
- Estimated Expiration
- 2034-12-09
AI Technical Summary
Traditional peptide synthesis requires a lot of manual operation, which leads to high labor costs and difficulty in accurately controlling the amount and timing of liquid injection, thus affecting synthesis efficiency and effectiveness.
It employs a liquid injection assembly, including multiple injection components, dispensing components, and switching valves, to achieve precise liquid injection and discharge through automated control. Combined with a floating mechanism and detectors, it ensures safety and accuracy.
It achieves zero-manual operation, reduces labor costs, improves work efficiency, and enables precise control of liquid injection volume and timing, thereby enhancing peptide synthesis results.
Smart Images
Figure CN223570657U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to polypeptide synthesis technical field, specifically, it relates to a kind of liquid injection subassembly and the polypeptide synthesis equipment with it. BACKGROUND
[0002] As a key field of biological medicine innovation research and development, polypeptide synthesis technology has broad development prospects. With its high biological activity, high specificity, lower side effects and not easy to accumulate in the body, etc. Advantages, it has become a global new drug research and development hotspot. At present, more than 80 kinds of polypeptide drugs have been approved for listing in the world, and the market size reached 62.8 billion US dollars in 2020, and is expected to grow to 96 billion US dollars in 2025, showing strong growth momentum.
[0003] The traditional reaction container for polypeptide synthesis has resin placed inside. The resin can be used as a solid carrier, which is usually pre-activated and has a protective group such as Fmoc (fluorenylmethoxycarbonyl). Then, 20% piperidine solution needs to be manually injected into the reaction container, which can deprotect the resin and remove the N-terminal Fmoc protecting group. After the reaction, the piperidine container needs to be drained, and then dimethylformamide (DMF) is manually injected into the reaction container to clean the piperidine solution on the inner wall of the reaction container. Then, subsequent processes can be carried out, and finally all amino acids are connected to the resin in the predetermined order to form a complete polypeptide chain. Finally, the liquid discharge assembly of the polypeptide synthesis equipment generates negative pressure, thereby pumping out the waste liquid in the reaction container.
[0004] The above-mentioned polypeptide synthesis requires a large amount of manual work, resulting in high labor cost. Moreover, due to human factors, the injection amount and timing of piperidine solution and DMF cannot be precisely controlled, resulting in poor polypeptide synthesis effect and low efficiency. UTILITY MODEL CONTENTS
[0005] In order to at least partially solve the problems existing in the prior art, according to one aspect of the utility model, a liquid injection assembly is provided. The liquid injection assembly includes a plurality of liquid injection members, a liquid outlet member, and a plurality of switching valves corresponding to the plurality of liquid injection members. The switching valves are arranged between the corresponding liquid injection members and the plurality of liquid outlet members. The switching valves have a liquid suction state that allows the corresponding liquid injection members to communicate with the liquid storage member, and a liquid injection state that allows the corresponding liquid injection members to communicate with the liquid outlet member. The liquid injection members are used to suck the liquid in the liquid storage member into the liquid injection members when the corresponding switching valves are in the liquid suction state, and to inject the liquid in the liquid injection members into the reaction container below the liquid outlet member through the liquid outlet member when the corresponding switching valves are in the liquid injection state.
[0006] For example, the switching valve has a first interface, a second interface and a third interface. The liquid injection component is connected to the first interface of its corresponding switching valve, the liquid outlet component is connected to the second interface, and the third interface is used to connect to the liquid storage component. When the switching valve is in the liquid suction state, the first interface and the third interface are connected. When the switching valve is in the liquid injection state, the first interface and the second interface are connected.
[0007] For example, the injection device includes a motor and a syringe connected to the drive end of the motor. The syringe is connected to a switching valve. When the switching valve is in the suction state, the motor is used to draw liquid from the reservoir into the syringe, and when the switching valve is in the injection state, the motor is used to inject the liquid from the syringe into the reaction vessel through the dispensing device.
[0008] For example, the liquid injection assembly also includes a floating mechanism, on which a liquid outlet is disposed. The liquid outlet can rise under the pressure of the reaction vessel and can fall after being removed from the reaction vessel. The floating mechanism controls the liquid injection assembly to stop when the liquid outlet rises to a predetermined position.
[0009] For example, the floating mechanism includes a main body, a floating element, a detector, and a controller. The floating element is vertically and elliptically mounted on the main body, and the liquid outlet is mounted on the floating element. The floating element is also provided with a sensing plate. The detector detects the sensing plate when the liquid outlet rises to a predetermined position and sends a control signal to the controller. The controller is used to control the liquid injection assembly to stop based on the control signal.
[0010] For example, the detector includes a transmitter and a receiver spaced apart, with a sensing element positioned between the transmitter and receiver when the liquid outlet rises to a predetermined position to block the receiver from receiving light emitted by the transmitter.
[0011] For example, the liquid outlet component includes a plurality of first liquid outlet needles, and the sidewall of the first liquid outlet needle is provided with a first liquid outlet hole that is inclined downward.
[0012] For example, along the circumferential direction of the first dispensing needle, a plurality of first dispensing holes are provided at intervals on the side wall of the first dispensing needle.
[0013] For example, along the extension direction of the first dispensing needle, a plurality of first dispensing holes are provided at intervals on the side wall of the first dispensing needle.
[0014] For example, the liquid outlet includes a plurality of second liquid outlet needles, each second liquid outlet needle including a needle tube and an umbrella-shaped cover extending obliquely downward from the side wall of the needle tube. The needle tube is provided with a second liquid outlet hole, and the umbrella-shaped cover covers and protrudes downward from the second liquid outlet hole.
[0015] For example, the liquid outlet further includes a liquid receiving tank drive and a liquid receiving tank connected to the drive end of the liquid receiving tank drive. The liquid receiving tank moves horizontally between a liquid receiving position located below the umbrella-shaped cover and a spaced position spaced apart from the umbrella-shaped cover under the drive of the liquid receiving tank drive.
[0016] Exemplarily, the liquid outlet member further comprises a second liquid outlet needle driving member, a driving end of the second liquid outlet needle driving member being connected to the second liquid outlet needle for driving the second liquid outlet needle to lift.
[0017] Exemplarily, the liquid injection assembly further comprises a first horizontal driving member, a second horizontal driving member and a vertical driving member, a driving end of the vertical driving member being connected to the liquid outlet member for driving the liquid outlet member to lift along a vertical direction, a driving end of the second horizontal driving member being connected to a main body of the vertical driving member for driving the vertical driving member and the liquid outlet member to move along a second horizontal direction, a driving end of the first horizontal driving member being connected to a main body of the second horizontal driving member for driving the second horizontal driving member, the vertical driving member and the liquid outlet member to move along a first horizontal direction, the first horizontal direction, the second horizontal direction and the vertical direction being perpendicular to each other.
[0018] Exemplarily, the liquid injection assembly further comprises a mounting plate, the liquid injection member and the switching valve being arranged on the mounting plate.
[0019] According to another aspect of the present application, there is also provided a polypeptide synthesis device. The polypeptide synthesis device comprises any one of the liquid injection assemblies as described above.
[0020] The liquid injection assembly provided by the embodiments of the present application can automatically inject liquid into the reaction container, thereby eliminating the need for manual operation. In this way, the liquid injection assembly can avoid labor costs and improve work efficiency. Moreover, since there is no human factor, the liquid injection assembly can accurately control the injection amount and injection timing of the liquid injected into the reaction container, thereby improving the effect of the relevant reaction performed in the reaction container. Furthermore, the plurality of liquid injection members can increase the injection amount. Compared with the structure for increasing the injection amount by using a large-sized liquid injection member, each of the plurality of liquid injection members can be relatively small in size, and the structure is relatively more precise, thereby facilitating accurate control of the injection amount.
[0021] A series of simplified forms are introduced in the utility model content, which will be further described in detail in the specific embodiment part. The utility model content part does not mean trying to limit the key features and necessary technical features of the claimed technical solution, and even less means trying to determine the protection scope of the claimed technical solution.
[0022] The advantages and features of the present application will be described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS
[0023] The following drawings of the present application are hereby incorporated as part of the present application for understanding the present application. The embodiments of the present application and the description thereof shown in the drawings are used to explain the principles of the present application. In the drawings,
[0024] Figure 1 FIG. 1 is a perspective view of a polypeptide synthesis apparatus according to an exemplary embodiment of the present application;
[0025] Figure 2 FIG. 2 is a perspective view of a polypeptide synthesis apparatus according to an exemplary embodiment of the present application; Figure 1 FIG. 3 is an enlarged view of a partial portion of the polypeptide synthesis apparatus shown in FIG. 2;
[0026] Figure 3 FIG. 4 is a perspective view of a liquid injection assembly according to an exemplary embodiment of the present application; Figure 1 FIG. 5 is a perspective view of a liquid injection assembly according to an exemplary embodiment of the present application;
[0027] Figure 4 FIG. 6 is a perspective view of a partial portion of a liquid injection assembly according to an exemplary embodiment of the present application; Figure 3 FIG. 7 is a perspective view of a partial portion of a liquid injection assembly according to an exemplary embodiment of the present application;
[0028] Figure 5 FIG. 8 is a front view of a first liquid outlet needle according to an exemplary embodiment of the present application; Figure 4 FIG. 9 is a sectional view of the first liquid outlet needle shown in FIG. 8; FIG. 10 is a sectional view of a second liquid outlet needle according to an exemplary embodiment of the present application; and
[0029] FIG. 11 is a sectional view of the second liquid outlet needle shown in FIG. 10. Figure 6 Figure 5 In the following description, numerous specific details are provided in order to provide a thorough understanding of the present application. One of ordinary skill in the art will realize, however, that the application can be practiced without one or more of these details. In other instances, well-known features have not been described in detail in order to not unnecessarily obscure the present application.
[0030] Figure 7 FIG. 1 is a perspective view of a polypeptide synthesis apparatus according to an exemplary embodiment of the present application; Figure 4 FIG. 10 is a sectional view of a second liquid outlet needle according to an exemplary embodiment of the present application; and
[0031] In the following description, numerous specific details are provided in order to provide a thorough understanding of the present application. One of ordinary skill in the art will realize, however, that the application can be practiced without one or more of these details. In other instances, well-known features have not been described in detail in order to not unnecessarily obscure the present application.
[0032] 100, liquid injection assembly; 200, liquid injection member; 210, motor; 220, needle cylinder; 300, liquid outlet member; 310, first liquid outlet needle; 311, first liquid outlet hole; 320, second liquid outlet needle; 321, needle tube; 322, umbrella-shaped cover; 323, second liquid outlet hole; 330, liquid receiving groove driving member; 340, liquid receiving groove; 350, second liquid outlet needle driving member; 400, switching valve; 410, first interface; 420, second interface; 430, third interface; 500, floating mechanism; 510, main body; 520, floating member; 521, inductive sheet; 530, detector; 531, transmitter; 532, receiver; 610, first horizontal driving member; 620, second horizontal driving member; 630, vertical driving member; 700, mounting plate; 800, liquid discharge container; 900, reaction container. DETAILED DESCRIPTION
[0033] In the following description, numerous specific details are provided in order to provide a thorough understanding of the present application. One of ordinary skill in the art will realize, however, that the application can be practiced without one or more of these details. In other instances, well-known features have not been described in detail in order to not unnecessarily obscure the present application.
[0034] According to an aspect of the present application, a liquid injection assembly is provided. The liquid injection assembly can be used to inject liquid into a reaction vessel. The liquid injection assembly can be applied to any suitable device, including but not limited to a polypeptide synthesis device. Therefore, according to another aspect of the present application, a polypeptide synthesis device is also provided. The liquid injection assembly and the polypeptide synthesis device according to the embodiments of the present application will be described in detail below with reference to the accompanying drawings.
[0035] As shown in Figures 1-4 The liquid injection assembly 100 can include a plurality of liquid injection members 200, a liquid outlet member 300, and a plurality of switching valves 400. The liquid outlet member 300 can be provided in one-to-one correspondence with the plurality of switching valves 400. Each switching valve 400 can be provided between its corresponding liquid injection member 200 and the liquid outlet member 300. In the embodiment shown in the figure, the liquid injection member 200 and the switching valve 400 can each include four.
[0036] The switching valve 400 can have a liquid suction state and a liquid injection state. The switching valve 400 can be switched between the liquid suction state and the liquid injection state. The switching valve 400 includes but is not limited to an electromagnetic switching valve, a manual switching valve, or a pneumatic switching valve. When the switching valve 400 is in the liquid suction state, its corresponding liquid injection member 200 can be made to communicate with a liquid storage member. The liquid storage member can be used to store liquid. The liquid includes but is not limited to DMF and / or piperidine solution. Also, when the switching valve 400 is in the liquid suction state, its corresponding liquid injection member 200 can be used to suck the liquid in the liquid storage member into the liquid injection member 200. When the switching valve 400 is in the liquid injection state, its corresponding liquid injection member 200 can be made to communicate with the liquid outlet member 300. Also, when the switching valve 400 is in the liquid injection state, its corresponding liquid injection member 200 can be used to inject the liquid in the liquid injection member 200 into a reaction vessel 900 below the liquid outlet member 300 through the liquid outlet member 300. The liquid injection member 200 includes but is not limited to a pump and a liquid injection container connected to the pump, or any suitable structure such as a negative pressure tank, as long as the above functions can be achieved. The liquid outlet member 300 includes but is not limited to a liquid outlet pipe, a liquid outlet needle, or any other suitable structure, as long as the above functions can be achieved.
[0037] In actual application, the switching valve 400 can be switched to the liquid suction state. In this way, the liquid injection member 200 can be in communication with the liquid storage member through the switching valve 400. The liquid injection member 200 can suck out the liquid in the liquid storage member, so that the liquid can be sucked into the liquid injection member 200. Then, the switching valve 400 can be switched to the liquid injection state. In this way, the liquid injection member 200 can be in communication with the liquid outlet member 300 through the switching valve 400. The liquid injection member 200 can discharge the liquid therein, so that the liquid can be injected into the reaction container 900 below the liquid outlet member 300 through the liquid outlet member 300. The liquid injected into the reaction container 900 can be used for reaction with the material in the reaction container 900. In the embodiment in which the liquid injection assembly 100 is applied to the polypeptide synthesis device, the liquid injected into the reaction container 900 can be used for polypeptide synthesis reaction with the material in the reaction container 900, so as to synthesize polypeptides.
[0038] In summary, the liquid injection assembly 100 provided by the embodiments of the present application can automatically inject liquid into the reaction container 900, so that manual operation is not required. In this way, the liquid injection assembly 100 can avoid labor costs and improve work efficiency. Moreover, without the influence of human factors, the liquid injection assembly 100 can accurately control the injection amount and injection timing of the liquid injected into the reaction container 900, so as to improve the effect of the related reaction in the reaction container 900. Moreover, the plurality of liquid injection members 200 can increase the injection amount. Compared with the structure for increasing the injection amount by using a large-specification liquid injection member, each of the plurality of liquid injection members 200 can be relatively small in specification, and the structure is relatively more precise, so that accurate control of the injection amount can be facilitated.
[0039] Exemplarily, as shown in Figures 1-2 The liquid injection member 200 can be connected to the first interface 410 of the corresponding switching valve 400 one by one. The liquid outlet member 300 can be connected to the second interface 420. Exemplarily, the liquid outlet member 300 can be connected to the second interface 420 of all the switching valves 400. The third interface 430 can be used for connection to the liquid storage member. When the switching valve 400 is in the liquid suction state, the first interface 410 and the third interface 430 can be in communication. In this way, the liquid injection member 200 can be used to sequentially pass the liquid in the liquid storage member through the third interface 430 and the first interface 410, so that the liquid injection member 200 can be sucked. When the switching valve 400 is in the liquid injection state, the first interface 410 and the second interface 420 can be in communication. In this way, the liquid injection member 200 can discharge the liquid therein, so that the liquid can sequentially pass through the first interface 410, the second interface 420 and the liquid outlet member 300, and then be injected into the reaction container 900 below the liquid outlet member 300. In this way, the structure of the switching valve 400 is simple, and the manufacturing cost is low.
[0040] Exemplarily, as shown in Figures 1-2 The liquid injection member 200 can include a motor 210 and a syringe 220. The driving end of the motor 210 can be connected to the syringe 220. The syringe 220 can be connected to its corresponding switching valve 400. Specifically, the syringe 220 can be connected to the first interface 410 of its corresponding switching valve 400. The motor 210 can be used to drive the piston rod of the syringe 220 to move to perform liquid suction and liquid discharge. Specifically, when the switching valve 400 is in the liquid suction state, the motor 210 can be used to suck the liquid in the liquid storage member into the syringe 220. When the switching valve 400 is in the liquid injection state, the motor 210 can be used to inject the liquid in the syringe 220 into the reaction container 900 through the liquid outlet member 300. The motor 210 can adopt various types of motors known in the art or possibly to appear in the future, including but not limited to servo motors or stepper motors. In this way, the structure of the liquid injection member 200 is simple, and the precision of liquid suction and liquid discharge is high, so that the injection amount can be accurately controlled.
[0041] Exemplarily, as shown in Figures 1-2 The liquid injection assembly 100 can further include a mounting plate 700. The mounting plate 700 can serve as the base of the liquid injection assembly 100. The mounting plate 700 can be used to connect to the components outside the liquid injection assembly 100, so as to be connected with the components. In the embodiment in which the liquid injection assembly 100 is applied to the polypeptide synthesis device, the mounting plate 700 can be used to connect to the rack of the polypeptide synthesis device. The liquid injection member 200 and the switching valve 400 can be arranged on the mounting plate 700 in a spaced manner. In this way, the liquid injection member 200 and its corresponding switching valve 400 can be adjacent, so as to facilitate connection. Exemplarily, the first interface 410 of the switching valve 400 can face its corresponding liquid injection member 200. Exemplarily, the mounting plate 700 can be a long strip plate extending in a straight line direction. The liquid injection member 200 and the switching valve 400 can be arranged on the mounting plate 700 in a spaced manner along the straight line direction.
[0042] Exemplarily, as shown in Figures 3-4As shown, the liquid injection assembly 100 may also include a floating mechanism 500. The liquid outlet 300 can be mounted on the floating mechanism 500. By using the floating mechanism 500, the liquid outlet 300 can be raised and lowered. Since the reaction vessel 900 is located below the liquid outlet 300, when the reaction vessel 900 and the liquid outlet 300 come into contact, the liquid outlet 300 can rise under the pressure of the reaction vessel 900. Furthermore, when the liquid outlet 300 detaches from the reaction vessel 900, it can descend under gravity, thus returning to its initial position. When the liquid outlet 300 rises to a predetermined position, the floating mechanism 500 can control the liquid injection assembly 100 to stop. With this configuration, in practical applications, if the liquid outlet 300 contacts the reaction vessel 900, it indicates that the liquid outlet 300 and the reaction vessel 900 are not aligned. If the liquid injection assembly 100 continues to operate normally at this time, the liquid through the liquid outlet 300 may flow outside the reaction vessel 900, potentially causing a safety accident. By incorporating a floating mechanism 500, when the dispensing component 300 rises to a predetermined position, the floating mechanism 500 can control the injection assembly 100 to stop. This prevents liquid from flowing out through the dispensing component 300, thus avoiding a safety accident. The stopping of the injection assembly 100 may include stopping the injection component 200 and / or switching the switching valve 400 to a suction state. For example, the floating mechanism 500 may be equipped with a button. When the dispensing component 300 rises to the predetermined position, it will abut against the button, thereby controlling the injection assembly 100 to stop.
[0043] For example, such as Figures 3-4As shown, the floating mechanism 500 can include a main body 510, a floating member 520, a detector 530, and a controller (not shown). The floating member 520 is arranged on the main body 510 in a liftable manner. The liquid outlet member 300 can be arranged on the floating member 520. The floating member 520 can further be provided with a sensing sheet 521. In this way, under the abutment of the reaction container 900, the liquid outlet member 300 can drive the floating member 520 and the sensing sheet 521 to ascend relative to the main body 510. When the liquid outlet member 300 is separated from the reaction container 900, the liquid outlet member 300 can drive the floating member 520 and the sensing sheet 521 to descend relative to the main body 510 under the action of gravity. When the liquid outlet member 300 ascends to a predetermined position, the detector 530 can detect the sensing sheet 521 and send a control signal to the controller. The controller can be used to control the liquid injection assembly 100 to stop based on the control signal. The detector 530 can include, but is not limited to, a laser sensor or an infrared sensor, as long as the above functions can be achieved. The controller can be built with electronic elements such as timers, comparators, registers, digital logic circuits, or realized with processor chips such as single-chip microcomputers, microprocessors, programmable logic controllers (PLC), digital signal processors (DSP), field programmable gate arrays (FPGA), programmable logic arrays (PLA), application-specific integrated circuits (ASIC), and their peripheral circuits. The structure of the floating mechanism 500 is relatively simple, the manufacturing cost is low, and the performance is stable.
[0044] As shown in the embodiment, Figures 3-4 The detector 530 can include a transmitter 531 and a receiver 532. The transmitter 531 and the receiver 532 can be arranged at intervals. When the liquid outlet member 300 ascends to a predetermined position, the sensing sheet 521 can be located between the transmitter 531 and the receiver 532. In this way, the sensing sheet 521 can block the receiver 532 from receiving the light emitted by the transmitter 531. At this time, the detector 530 can send a control signal to the controller. The controller can be used to control the liquid injection assembly 100 to stop based on the control signal. In this way, the structure of the detector 530 is relatively simple, and the sensing sheet 521 will not be contacted, so that the problems such as interference will not occur.
[0045] As shown in the embodiment, Figures 3-4 The liquid outlet member 300 can include a plurality of first liquid outlet needles 310, such as two, three, or more. In the embodiment shown in the figure, the first liquid outlet needle 310 can include six. The plurality of first liquid outlet needles 310 can be arranged in any suitable manner. The reaction container 900 can be arranged below the plurality of first liquid outlet needles 310. The plurality of first liquid outlet needles 310 can be used to inject liquid into the same reaction container 900, thereby improving the amount of liquid injection. As shown in the embodiment, Figures 5-6As shown, the sidewall of each first liquid outlet needle 310 can be provided with one or more first liquid outlet holes 311. The first liquid outlet hole 311 can be a circular hole or any other suitable shape. The first liquid outlet hole 311 can be inclined downward. When the switching valve 400 is in the liquid injection state, the corresponding liquid injection member 200 can be used to inject the liquid in the liquid injection member 200 into the reaction vessel 900 below the first liquid outlet needle 310 through the first liquid outlet hole 311. Since the first liquid outlet hole 311 is inclined downward, the liquid passing therethrough can flow downward, so as to contact and flow downward along the inner sidewall of the reaction vessel 900. In this way, the liquid can be used to clean the inner sidewall of the reaction vessel 900. In the embodiment in which the liquid injection assembly 100 is applied to the polypeptide synthesis device, the liquid passing through the first liquid outlet needle 310 can include DMF. The DMF can clean the piperidine solution on the inner sidewall of the reaction vessel 900, so as to improve the cleanliness of the reaction vessel 900, thereby facilitating the subsequent process.
[0046] Exemplarily, as shown in Figs. 6 and 7, the sidewall of the first liquid outlet needle 310 can be provided with a plurality of first liquid outlet holes 311, for example, two, three or more, along the circumferential direction of the first liquid outlet needle 310. In this way, the liquid passing through the first liquid outlet hole 311 can clean a plurality of positions along the circumferential direction of the inner sidewall of the reaction vessel 900, so as to improve the cleaning effect. Figures 5-6
[0047] Exemplarily, as shown in Figs. 6 and 7, the sidewall of the first liquid outlet needle 310 can be provided with a plurality of first liquid outlet holes 311, for example, two, three or more, along the circumferential direction of the first liquid outlet needle 310. In this way, the liquid passing through the first liquid outlet hole 311 can clean a plurality of positions along the circumferential direction of the inner sidewall of the reaction vessel 900, so as to improve the cleaning effect. Figures 5-6
[0048] Exemplarily, as shown in Figs. 6 and 7, the sidewall of the first liquid outlet needle 310 can be provided with a plurality of first liquid outlet holes 311, for example, two, three or more, along the circumferential direction of the first liquid outlet needle 310. In this way, the liquid passing through the first liquid outlet hole 311 can clean a plurality of positions along the circumferential direction of the inner sidewall of the reaction vessel 900, so as to improve the cleaning effect. Figures 3-4 Figure 7 As shown, each second dispensing needle 320 may include a needle tube 321 and an umbrella-shaped cover 322. The umbrella-shaped cover 322 may extend obliquely downward from the side wall of the needle tube 321. A second dispensing hole 323 may be provided on the needle tube 321. The umbrella-shaped cover 322 may cover and protrude downward from the second dispensing hole 323. When the switching valve 400 is in the dispensing state, its corresponding dispensing member 200 can be used to inject the liquid in the dispensing member 200 into the reaction vessel 900 below the second dispensing needle 320 through the second dispensing needle 320. Specifically, the liquid can flow down along the inner side wall of the umbrella-shaped cover 322 through the second dispensing hole 323 of the needle tube 321. With this configuration, the liquid through the second dispensing needle 320 can enter the reaction vessel 900 in a shower-like manner, thereby reducing impact and suppressing liquid splashing onto the inner side wall of the reaction vessel 900, ensuring the cleanliness of the reaction vessel 900. In an embodiment where the liquid injection assembly 100 is applied to a peptide synthesis apparatus, the liquid dispensed through the second dispensing needle 320 may include a piperidine solution. The piperidine solution is required to be kept off the inner wall of the reaction vessel 900 to prevent contamination of subsequent processes.
[0049] For example, such as Figures 3-4 As shown, the liquid dispensing component 300 may further include a liquid receiving tank drive 330 and a liquid receiving tank 340. The drive end of the liquid receiving tank drive 330 can be connected to the liquid receiving tank 340. Driven by the liquid receiving tank drive 330, the liquid receiving tank 340 can move horizontally between a liquid receiving position and an intermittent position. The liquid receiving tank drive 330 includes, but is not limited to, a motor, a cylinder, or a hydraulic cylinder. When the liquid receiving tank 340 is in the liquid receiving position, it can be located below the umbrella-shaped cover 322. In this way, the liquid receiving tank 340 can collect droplets dripping from the umbrella-shaped cover 322, preventing the droplets from falling into the reaction vessel 900 below. In an embodiment where the liquid injection component 100 is applied to a peptide synthesis device, the second liquid dispensing needle 320 can serve as a backup liquid dispensing solution. That is, the second liquid dispensing needle 320 is not usually used. Therefore, by placing the liquid receiving tank 340 in the liquid receiving position, droplets dripping from the second liquid dispensing needle 320 can be prevented from affecting related processes. When the receiving tank 340 is in the spaced-out position, it can be horizontally spaced from the umbrella-shaped cover 322. In this way, the injection unit 200 can be used to inject the liquid in the injection unit 200 into the reaction vessel 900 below the second liquid outlet needle 320 through the second liquid outlet needle 320, and the receiving tank 340 will not cause obstruction.
[0050] For example, the dispensing component 300 may simultaneously include multiple first dispensing needles 310 and multiple second dispensing needles 320. The first dispensing needles 310 and the second dispensing needles 320 may each carry different liquids. Multiple dispensing components 200 may be connected to all the first dispensing needles 310 and second dispensing needles 320 via a switching valve 400; alternatively, some dispensing components 200 may be connected to the first dispensing needles 310 via the switching valve 400, while other dispensing components 200 may be connected to the second dispensing needles 320 via the switching valve 400.
[0051] For example, such as Figures 3-4 As shown, the liquid outlet component 300 may further include a second liquid outlet needle drive component 350. The drive end of the second liquid outlet needle drive component 350 can be connected to the second liquid outlet needle 320 to drive the second liquid outlet needle 320 to move up and down. The second liquid outlet needle drive component 350 includes, but is not limited to, a motor, a cylinder, or a hydraulic cylinder. The second liquid outlet needle drive component 350 can drive the second liquid outlet needle 320 to extend downward into the reaction vessel 900, thereby reducing the distance between the umbrella-shaped cover 322 and the liquid surface inside the reaction vessel 900, thus minimizing impact and suppressing liquid splashing onto the inner wall of the reaction vessel 900. After the liquid injection is completed, the second liquid outlet needle drive component 350 can drive the second liquid outlet needle 320 to extend upward out of the reaction vessel 900. In embodiments where the liquid outlet component 300 includes a liquid receiving trough drive component 330 and a liquid receiving trough 340, when the liquid receiving trough 340 is in the spaced-out position, the second liquid outlet needle drive component 350 can be used to drive the second liquid outlet needle 320 to move up and down. In an embodiment where the liquid injection assembly 100 includes a floating mechanism 500, during the process of the second liquid injection needle 320 being driven to descend by the second liquid injection needle drive 350, if the second liquid injection needle 320 comes into contact with the reaction container 900 due to misalignment, the floating mechanism 500 can control the liquid injection assembly 100 to stop when the liquid injection needle 300 rises to a predetermined position under the pressure of the reaction container 900.
[0052] For example, such as Figure 1 and 3As shown, the liquid injection assembly 100 can further include a first horizontal driving member 610, a second horizontal driving member 620, and a vertical driving member 630. A driving end of the vertical driving member 630 can be connected to the liquid outlet member 300 for driving the liquid outlet member 300 to move up and down along a vertical direction. A driving end of the second horizontal driving member 620 can be connected to a main body of the vertical driving member 630 for driving the vertical driving member 630 and the liquid outlet member 300 to move along a second horizontal direction. A driving end of the first horizontal driving member 610 can be connected to a main body of the second horizontal driving member 620 for driving the second horizontal driving member 620, the vertical driving member 630, and the liquid outlet member 300 to move along a first horizontal direction. The first horizontal direction, the second horizontal direction, and the vertical direction can be perpendicular to each other. The first horizontal driving member 610, the second horizontal driving member 620, and the vertical driving member 630 can form a three-axis driving mechanism, so that the liquid outlet member 300 can be moved to more positions. The first horizontal driving member 610, the second horizontal driving member 620, and the vertical driving member 630 can include, but are not limited to, motors, air cylinders, or hydraulic cylinders. In actual applications, the first horizontal driving member 610 and the second horizontal driving member 620 can drive the vertical driving member 630 and the liquid outlet member 300 to move above the reaction container 900. Then, the vertical driving member 630 can drive the liquid outlet member 300 to move down, so as to be close to the reaction container 900 for liquid injection. In the embodiment in which the liquid outlet member 300 includes the second liquid outlet needle 320 and the second liquid outlet needle driving member 350, the vertical driving member 630 can drive the liquid outlet member 300 to move down to be close to the reaction container 900, and then the second liquid outlet needle driving member 350 can drive the second liquid outlet needle 320 to move further down to extend into the reaction container 900. Exemplarily, the liquid injection assembly 100 can further include a liquid discharge container 800. The liquid discharge container 800 can be arranged below the path along which the liquid outlet member 300 moves. In this way, the first horizontal driving member 610 and the second horizontal driving member 620 can drive the liquid outlet member 300 to move above the liquid discharge container 800. Then, the liquid injection member 200 can discharge liquid into the liquid discharge container 800 through the liquid outlet member 300, so as to discharge air in the pipeline between the liquid injection member 200 and the liquid outlet member 300. After the liquid discharge is completed, the first horizontal driving member 610 and the second horizontal driving member 620 can drive the vertical driving member 630 and the liquid outlet member 300 to move above the reaction container 900 for subsequent liquid injection operation. Since the air has been discharged, the accuracy of the liquid injection amount of the liquid injected by the liquid outlet member 300 into the reaction container 900 is higher at this time, so as to be used to improve the effect of the related reaction performed in the reaction container 900.
[0053] In the description of the utility model, it is understood that the orientation words such as "front", "rear", "upper", "lower", "left", "right", "transverse", "vertical", "perpendicular", "horizontal" and "top", "bottom" and the like indicated orientation or positional relationship is usually based on the orientation or positional relationship shown in the drawings, only for the convenience of describing the utility model and simplifying the description, in the absence of the opposite statement, these orientation words do not indicate and imply that the device or element indicated must have a particular orientation or be constructed and operated in a particular orientation, therefore, it can not be understood as the limitation of the protection scope of the utility model;The orientation words "inner", "outer" refer to the inner and outer of the contour of each component.
[0054] For the convenience of description, regional relative terms such as "on", "above", "upper surface", "upper" and the like can be used here to describe the regional position relationship of one or more components or features shown in the drawing with other components or features. It should be understood that the regional relative terms not only include the orientation of the components described in the drawing, but also include different orientations in use or operation. For example, if the components in the drawing are inverted as a whole, the components "above" or "on" other components or features will include the case of "below" or "under" other components or structures. Thus, the exemplary term "above" can include both "above" and "below". In addition, these components or features can also be positioned at other different angles (for example, rotated by 90 degrees or other angles), and all these cases are intended to be included herein.
[0055] It should be noted that the terms used herein are only for the purpose of describing specific embodiments, and are not intended to limit the exemplary embodiments according to the present application. As used herein, the singular form is intended to include the plural form unless the context clearly indicates otherwise, and furthermore, it should be understood that when the terms "comprise" and / or "include" are used in the specification, it means that the features, steps, operations, components, assemblies and / or their combinations are present.
[0056] It should be noted that the terms "first", "second" and the like in the specification and claims of the present application and the above-described drawings are used to distinguish similar objects, and do not necessarily describe a specific order or sequence. It should be understood that the data used in this way can be interchanged under appropriate circumstances, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.
[0057] The utility model discloses has been through the above embodiment has been explained, but should understand, the above embodiment is only for the purpose of example and illustration, and is not intended to limit the utility model to the range of described embodiment. In addition, those skilled in the art can understand that the utility model is not limited to the above embodiment, and more kinds of variations and modifications can be made according to the teaching of the utility model, and the variations and modifications all fall within the range of the utility model claimed. The protection scope of the utility model is defined by the attached claims and its equivalent range.
Claims
1. A liquid injection assembly, characterized by, The injection assembly comprises a plurality of liquid injection members, a liquid outlet member, and a plurality of switching valves corresponding to the liquid injection members one by one, the switching valves are arranged between the corresponding liquid injection members and the liquid outlet member, the switching valves have a liquid suction state for connecting the corresponding liquid injection members to the liquid storage member and a liquid injection state for connecting the corresponding liquid injection members to the liquid outlet member, the liquid injection members are used for sucking liquid in the liquid storage member into the liquid injection members when the corresponding switching valves are in the liquid suction state, and the liquid injection members are used for injecting liquid in the liquid injection members into a reaction container below the liquid outlet member through the liquid outlet member when the corresponding switching valves are in the liquid injection state.
2. The liquid injection assembly of claim 1, wherein, The switching valve has a first interface, a second interface and a third interface, the liquid injection member is connected to the first interface of the corresponding switching valve, the liquid outlet member is connected to the second interface, and the third interface is used for connecting to the liquid storage member, the first interface and the third interface are communicated when the switching valve is in the liquid suction state, and the first interface and the second interface are communicated when the switching valve is in the liquid injection state.
3. The liquid injection assembly of claim 1, wherein, The liquid injection member comprises a motor and a needle cylinder connected to the driving end of the motor, the needle cylinder is connected to the switching valve, the motor is used for sucking liquid in the liquid storage member into the needle cylinder when the switching valve is in the liquid suction state, and the motor is used for injecting liquid in the needle cylinder into the reaction container through the liquid outlet member when the switching valve is in the liquid injection state.
4. The liquid injection assembly of claim 1, wherein, The injection assembly further comprises a floating mechanism, the liquid outlet member is arranged on the floating mechanism, the liquid outlet member can rise when the reaction container is pressed and can fall after the reaction container is separated, and the floating mechanism controls the injection assembly to stop when the liquid outlet member rises to a predetermined position.
5. The liquid injection assembly of claim 4, wherein, The floating mechanism comprises a main body, a floating member, a detector and a controller, the floating member is arranged on the main body in a lifting manner, the liquid outlet member is arranged on the floating member, and an inductive sheet is further arranged on the floating member, the detector detects the inductive sheet when the liquid outlet member rises to the predetermined position, and sends a control signal to the controller, and the controller is used for controlling the injection assembly to stop based on the control signal.
6. The liquid injection assembly of claim 5, wherein, The detector comprises a transmitter and a receiver arranged at intervals, and the inductive sheet is located between the transmitter and the receiver when the liquid outlet member rises to the predetermined position, so as to interrupt the receiver from receiving light emitted by the transmitter.
7. The liquid injection assembly of claim 1, wherein, The liquid outlet member comprises a plurality of first liquid outlet needles, and a first liquid outlet hole is arranged on the side wall of the first liquid outlet needle in a downward inclined manner.
8. The injection assembly of claim 7, wherein, a plurality of first liquid outlet holes are arranged on the side wall of the first liquid outlet needle at intervals in the circumferential direction of the first liquid outlet needle; and / or a plurality of first liquid outlet holes are arranged on the side wall of the first liquid outlet needle at intervals in the extension direction of the first liquid outlet needle.
9. The liquid injection assembly of claim 1, wherein, The liquid outlet member comprises a plurality of second liquid outlet needles, each of the second liquid outlet needles comprises a needle tube and an umbrella-shaped cover extending downwardly and inclinedly from the side wall of the needle tube, a second liquid outlet hole is arranged on the needle tube, and the umbrella-shaped cover covers and protrudes downwardly from the second liquid outlet hole.
10. The liquid injection assembly of claim 9, wherein, The liquid outlet member further comprises a liquid receiving groove driving member and a liquid receiving groove connected to a driving end of the liquid receiving groove driving member, the liquid receiving groove moves along a horizontal direction under the driving of the liquid receiving groove driving member between a liquid receiving position below the umbrella-shaped cover and a spaced position spaced from the umbrella-shaped cover.
11. The liquid injection assembly of claim 9, wherein, The liquid outlet member further comprises a second liquid outlet needle driving member, a driving end of the second liquid outlet needle driving member is connected to the second liquid outlet needle for driving the second liquid outlet needle to lift and lower.
12. The liquid injection assembly of claim 1, wherein, The liquid injection assembly further comprises a first horizontal driving member, a second horizontal driving member and a vertical driving member, a driving end of the vertical driving member is connected to the liquid outlet member for driving the liquid outlet member to lift and lower along a vertical direction, a driving end of the second horizontal driving member is connected to a main body of the vertical driving member for driving the vertical driving member and the liquid outlet member to move along a second horizontal direction, a driving end of the first horizontal driving member is connected to a main body of the second horizontal driving member for driving the second horizontal driving member, the vertical driving member and the liquid outlet member to move along a first horizontal direction, the first horizontal direction, the second horizontal direction and the vertical direction are perpendicular to each other.
13. The liquid injection assembly of claim 1, wherein, The liquid injection assembly further comprises a mounting plate, the liquid injection member and the switching valve are arranged on the mounting plate.
14. A polypeptide synthesis apparatus, characterized in that, A liquid injection assembly comprising any one of claims 1-13.