Culture solution injection device for organoid culture

Abstract

The utility model discloses a culture solution injection device for organ-like culture, which comprises a box body, a support plate is fixedly connected in the box body, a quantitative box is fixedly mounted on the support plate, a rotating shaft is rotatably connected in the quantitative box, a motor is fixedly connected on one side of the outside of the quantitative box, and the rotating shaft is rotatably connected with the motor. The output end of the motor extends into the quantitative box and is fixedly connected with the rotating shaft, partition plates are fixedly connected to the outer portion of the rotating shaft at equal intervals, the sides, away from the rotating shaft, of the partition plates are attached to the inner wall of the quantitative box, protective sleeves are fixedly connected to the outer sides of the partition plates, and a plurality of telescopic rods are arranged on the outer side of the rotating shaft and located between the adjacent partition plates. According to the device, the dosage of a culture solution can be accurately provided through the cooperation of the rotating shaft, the partition plate and the adjustable telescopic rod, the culture consistency and repeatability are improved, the sealing performance can be guaranteed through the structure of the sliding plate, the sliding wheel and the spring, the position change can be adapted, the leakage risk is reduced, the service life of the device is prolonged, and the working efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to organoid culture technical field, especially organoid culture culture solution injection device for culture. BACKGROUND

[0002] In the field of organoid culture, accurate control of the injection amount of culture solution is crucial for the success and effectiveness of the culture.

[0003] Traditional culture solution injection devices often have problems such as inaccurate quantification and difficulty in adjusting the injection amount, which cannot meet the needs of organoid culture for precise supply of culture solution.

[0004] Therefore, we propose an organoid culture culture solution injection device. UTILITY MODEL CONTENT

[0005] The main purpose of the utility model is to provide an organoid culture culture solution injection device to prevent culture failure caused by inaccurate injection of culture solution, thereby improving the success rate and quality of organoid culture, and effectively solving the problems in the background art.

[0006] To achieve the above purpose, the technical scheme adopted by the utility model is as follows:

[0007] An organoid culture culture solution injection device, comprising a box body, a support plate fixedly connected inside the box body, a quantitative tank fixedly installed on the support plate, a rotating shaft rotatably connected inside the quantitative tank, a motor fixedly connected to one side of the outside of the quantitative tank, the output end of the motor extending into the inside of the quantitative tank and fixedly connected with the rotating shaft, equidistantly fixedly connected with a partition plate outside the rotating shaft, the side away from the rotating shaft of the partition plate being in close contact with the inner wall of the quantitative tank;

[0008] The outer side of the partition plate is fixedly connected with a protective sleeve, a plurality of telescopic rods are arranged outside the rotating shaft and between adjacent partition plates, one end of the telescopic rod away from the rotating shaft penetrates the protective sleeve and is fixedly connected with a first sliding plate, two second sliding plates arranged in parallel are slidably connected inside the first sliding plate, one end of the second sliding plate is provided with a pulley, a sliding groove for installing the pulley is formed in the outer side of the partition plate, and the end away from the pulley of the second sliding plate is connected with the inner wall of the first sliding plate through a spring.

[0009] By adopting the above technical scheme, the motor is started, the output end drives the rotating shaft to rotate in the quantitative tank, the equidistantly fixed partition plate outside the rotating shaft rotates, and the protective sleeve outside the partition plate plays a protective and sealing role.

[0010] During the rotation of the rotating shaft, the telescopic rods located between adjacent partitions will move accordingly. By changing the extension length of the telescopic rods, the size of the space between adjacent partitions for accommodating the culture solution can be adjusted to adapt to different quantitative requirements of the culture solution.

[0011] The second sliding plate inside the first sliding plate at one end of the telescopic rod slides in the sliding groove outside the partition through the pulley. At the same time, under the action of the spring, the second sliding plate can adapt to different positions and maintain good contact with the sliding groove, thereby ensuring the sealing of the space between the partitions.

[0012] Through the continuous rotation of the rotating shaft to drive the partitions, the culture solution can be quantitatively separated in the adjusted space between adjacent partitions, realizing the quantitative delivery of the culture solution, thereby accurately injecting the required amount of culture solution for organoid culture.

[0013] Further, the top end of the box body is fixedly installed with a liquid inlet hopper, and the top end of the quantitative tank is fixedly connected with a first conduit, and the first conduit is in communication with the liquid inlet hopper.

[0014] By adopting the above technical scheme, the liquid inlet hopper is used to receive the culture solution to be injected, so that the culture solution added from the liquid inlet hopper can smoothly enter the quantitative tank through the first conduit, preparing for subsequent quantitative delivery and injection operation.

[0015] Further, the bottom end inner wall of the box body is fixedly installed with a liquid storage tank, the bottom end of the quantitative tank is fixedly connected with a second conduit, the bottom end of the second conduit penetrates through the support plate and is fixedly installed at the top end of the liquid storage tank, and the second conduit is in communication with the inside of the liquid storage tank.

[0016] By adopting the above technical scheme, the culture solution quantitatively completed in the quantitative tank can smoothly flow into the liquid storage tank for storage through the second conduit.

[0017] Further, the bottom end of the liquid storage tank is fixedly connected with a liquid outlet pipe, the bottom end of the liquid outlet pipe penetrates through the box body and extends to the outside, and an electromagnetic valve is arranged on the liquid outlet pipe.

[0018] By adopting the above technical scheme, the electromagnetic valve is arranged on the liquid outlet pipe, and by controlling the opening and closing of the electromagnetic valve, it can be determined whether the culture solution in the liquid storage tank flows out or not, as well as the speed and flow of the culture solution, thereby realizing precise control of the output of the culture solution.

[0019] Further, a visual window is arranged on the side of the box body away from the motor and at the position of the quantitative tank.

[0020] By adopting the above technical scheme, through this visual window, the operator can intuitively observe the working condition inside the quantitative tank, such as the quantitative distribution process of the culture solution, the rotation state of the partitions, etc., so as to timely find out the problems and carry out corresponding operation and adjustment.

[0021] Further, the box is located on the same side of the visual window and the liquid storage tank is rotationally connected with a box door at the position, and a handle is welded outside the box door.

[0022] By adopting the technical scheme, the operator can conveniently open the box door by holding the handle to check, maintain or perform relevant operation on the liquid storage tank.

[0023] Compared with the prior art, the utility model has the following beneficial effects:

[0024] (1) The utility model relates to a culture solution injection device for organoid culture, which can accurately separate the culture solution in the space between adjacent partitions adjusted by the rotating shaft and the telescopic rod with adjustable length, provide the organoid culture with an accurate dose of culture solution, and help to improve the consistency and repeatability of the culture.

[0025] (2) The utility model relates to a culture solution injection device for organoid culture, the second sliding plate in the first sliding plate slides in the sliding groove through the pulley and keeps good contact under the action of the spring, guarantees the sealing property of the space between the partitions; meanwhile, the structure can adapt to the position change caused by the telescopic rod, makes the device stable and reliable during operation, reduces the risk of culture solution leakage, and improves the service life and working efficiency of the device. DETAILED DESCRIPTION

[0026] Figure 1 It is a structure schematic view of the utility model culture solution injection device for organoid culture.

[0027] Figure 2 It is an internal structure schematic view of the utility model culture solution injection device for organoid culture.

[0028] Figure 3 It is an internal structure schematic view of the utility model culture solution injection device for organoid culture. Figure 2 It is an enlarged view of A in the middle.

[0029] In the figure: 1, box; 2, dosing tank; 3, rotating shaft; 4, partition; 5, protective sleeve; 6, telescopic rod; 7, first sliding plate; 8, pulley; 9, sliding groove; 10, second sliding plate; 11, spring; 12, motor; 13, liquid inlet hopper; 14, first conduit; 15, support plate; 16, second conduit; 17, liquid storage tank; 18, liquid outlet pipe; 19, electromagnetic valve; 20, visual window; 21, box door; 22, handle. DETAILED DESCRIPTION

[0030] In order to make the technical means, creative features, purposes and effects of the utility model easy to understand, the utility model is further described below in combination with specific implementation manners.

[0031] In order to prevent the culture failure caused by the inaccurate injection amount of the culture solution, thereby improving the success rate and quality of the organoid culture, as shown in Figure 1 、 Figure 2 、 Figure 3 The culture solution injection device for organoid culture comprises a box body 1, a support plate 15 fixedly connected inside the box body 1, a quantitative tank 2 fixedly installed on the support plate 15, a rotating shaft 3 rotatably connected inside the quantitative tank 2, a motor 12 fixedly connected to one side of the outside of the quantitative tank 2, the output end of the motor 12 extending into the inside of the quantitative tank 2 and fixedly connected with the rotating shaft 3, a partition plate 4 fixedly connected with the rotating shaft 3 at equal intervals on the outside, and the side away from the rotating shaft 3 of the partition plate 4 being in close contact with the inner wall of the quantitative tank 2.

[0032] The outside of the partition plate 4 is fixedly connected with a protective sleeve 5, a plurality of telescopic rods 6 are arranged between the adjacent partition plates 4 on the outside of the rotating shaft 3, one end of the telescopic rod 6 away from the rotating shaft 3 penetrates through the protective sleeve 5 and is fixedly connected with a first sliding plate 7, the inside of the first sliding plate 7 is slidably connected with two second sliding plates 10 arranged in parallel, one end of the second sliding plate 10 is provided with a pulley 8, a sliding groove 9 for installing the pulley 8 is formed in the outside of the partition plate 4, and the end of the second sliding plate 10 away from the pulley 8 is connected with the inner wall of the first sliding plate 7 through a spring 11.

[0033] In use, the motor 12 is started, the output end of the motor 12 drives the rotating shaft 3 to rotate in the quantitative tank 2, the partition plates 4 fixedly connected with the rotating shaft 3 at equal intervals rotate, and the protective sleeve 5 on the outside of the partition plate 4 plays a protective and sealing role.

[0034] During the rotation of the rotating shaft 3, the telescopic rods 6 between the adjacent partition plates 4 move, by changing the extension length of the telescopic rods 6, the space size between the adjacent partition plates 4 for accommodating the culture solution can be adjusted to adapt to different quantitative requirements of the culture solution.

[0035] The second sliding plates 10 in the first sliding plate 7 at one end of the telescopic rod 6 slide in the sliding groove 9 outside the partition plate 4 through the pulley 8, and under the action of the spring 11, the second sliding plate 10 can adapt to different positions and keep good contact with the sliding groove 9, so as to ensure the sealing property of the space between the partition plates 4.

[0036] By continuously rotating the partition plates 4 through the rotating shaft 3, the culture solution can be quantitatively separated in the adjusted space between the adjacent partition plates 4, the quantitative delivery of the culture solution is realized, and the required amount of culture solution can be accurately injected for the organoid culture.

[0037] For example, as shown in Figure 1 、 Figure 2As shown, the utility model also includes, the top fixed mounting of box 1 is into liquid hopper 13, the top fixed connection of quantitative tank 2 has first pipe 14, and first pipe 14 is linked with into liquid hopper 13.

[0038] In use, into liquid hopper 13 is used to receive the culture solution to be injected, so that the culture solution added from into liquid hopper 13 can smoothly enter quantitative tank 2 through first pipe 14, and prepare for subsequent quantitative delivery and injection operation.

[0039] Exemplarily, as shown in the figure, Figure 2 As shown, the utility model also includes, the bottom end inner wall fixed mounting of box 1 is liquid storage tank 17, the bottom fixed connection of quantitative tank 2 has second pipe 16, the bottom of second pipe 16 penetrates support plate 15 and is fixedly installed at the top of liquid storage tank 17, and is linked with the inside of liquid storage tank 17.

[0040] In use, the culture solution of quantitative completion in quantitative tank 2 can flow into liquid storage tank 17 for storage through second pipe 16.

[0041] Exemplarily, as shown in the figure, Figure 1 、 Figure 2 As shown, the utility model also includes, the bottom fixed connection of liquid storage tank 17 has liquid outlet pipe 18, the bottom of liquid outlet pipe 18 penetrates box 1 and extends to outside, and liquid outlet pipe 18 is equipped with solenoid valve 19.

[0042] In use, solenoid valve 19 is arranged on liquid outlet pipe 18, by controlling the switch of solenoid valve 19, can decide the outflow of culture solution in liquid storage tank 17 and the speed and flow of outflow, to realize the accurate control of culture solution output.

[0043] Exemplarily, as shown in the figure, Figure 1 As shown, the utility model also includes, the side of box 1 away from motor 12 and located the position of quantitative tank 2 is equipped with visible window 20.

[0044] In use, through this visible window 20, operating personnel can intuitively observe the working condition inside quantitative tank 2, such as the quantitative distribution process of culture solution, the rotation state of baffle, to find problems in time and carry out corresponding operation and adjustment.

[0045] Exemplarily, as shown in the figure, Figure 1 As shown, the utility model also includes, the rotation connection of box 1 at the same side of visible window 20 and the position of liquid storage tank 17 has box door 21, and the outside welding of box door 21 has handle 22.

[0046] In use, operating personnel can conveniently open box door 21 by holding handle 22, and check, maintain or relevant operation to liquid storage tank 17.

[0047] It should be noted that the utility model is a kind of organoid culture with culture solution injection device, pour the culture solution to be injected into liquid inlet hopper 13, culture solution flows into quantitative tank 2 by first conduit 14;

[0048] Start motor 12, motor drives rotating shaft 3 rotation, according to the amount of required culture solution, adjust the telescopic length of telescopic rod 6, change the space size between adjacent partition plate 4, rotating shaft 3 drives partition plate 4 continuous rotation, culture solution is quantitatively separated in the space of adjacent partition plate 4 adjusted, realizes quantitative delivery;

[0049] Quantitative culture solution flows into liquid storage tank 17 storage by second conduit 16, according to the culture demand, by the switch of solenoid valve 19, decide whether the outflow of culture solution in liquid storage tank 17, outflow speed and flow.

[0050] The basic principle and main features of the utility model and the advantages of the utility model are shown and described above. Those skilled in the art should understand that the utility model is not limited to the above examples, and the above examples and descriptions in the specification are only to illustrate the principles of the utility model

[0051] The utility model also has various changes and improvements without departing from the spirit and scope of the utility model, and these changes and improvements fall within the scope of the utility model claimed. The scope of protection of the utility model is defined by the appended claims and their equivalents.

Claims

1. A culture medium injection device for organoid culture, comprising a housing (1), characterized in that, A support plate (15) is fixedly connected inside the box (1), and a metering box (2) is fixedly installed on the support plate (15). A rotating shaft (3) is rotatably connected inside the metering box (2). A motor (12) is fixedly connected to one side of the outside of the metering box (2). The output end of the motor (12) extends into the inside of the metering box (2) and is fixedly connected to the rotating shaft (3). A partition plate (4) is fixedly connected at equal intervals to the outside of the rotating shaft (3). The side of the partition plate (4) away from the rotating shaft (3) is in contact with the inner wall of the metering box (2). A protective sleeve (5) is fixedly connected to the outside of the partition (4). Multiple telescopic rods (6) are provided on the outside of the rotating shaft (3) and between adjacent partitions (4). The end of the telescopic rod (6) away from the rotating shaft (3) passes through the protective sleeve (5) and is fixedly connected to a first sliding plate (7). Two relatively parallel second sliding plates (10) are slidably connected inside the first sliding plate (7). A pulley (8) is provided at one end of the second sliding plate (10). A groove (9) for installing the pulley (8) is opened on the outside of the partition (4). The end of the second sliding plate (10) away from the pulley (8) is connected to the inner wall of the first sliding plate (7) by a spring (11).

2. The culture medium injection device for organoid culture according to claim 1, characterized in that: The top of the box (1) is fixedly installed with a liquid inlet (13), and the top of the metering box (2) is fixedly connected with a first conduit (14), and the first conduit (14) is connected to the liquid inlet (13).

3. The culture medium injection device for organoid culture according to claim 1, characterized in that: A liquid storage tank (17) is fixedly installed on the inner wall of the bottom end of the box (1), and a second conduit (16) is fixedly connected to the bottom end of the metering box (2). The bottom end of the second conduit (16) passes through the support plate (15) and is fixedly installed on the top of the liquid storage tank (17), and is connected to the inside of the liquid storage tank (17).

4. The culture medium injection device for organoid culture according to claim 3, characterized in that: The bottom end of the liquid storage tank (17) is fixedly connected to the liquid outlet pipe (18), the bottom end of the liquid outlet pipe (18) extends through the tank body (1) to the outside, and the liquid outlet pipe (18) is equipped with a solenoid valve (19).

5. The culture medium injection device for organoid culture according to claim 1, characterized in that: A viewing window (20) is provided on the side of the box (1) away from the motor (12) and at the position of the metering box (2).

6. The culture medium injection device for organoid culture according to claim 5, characterized in that: The box body (1) is located on the same side of the viewing window (20) and at the position of the liquid storage tank (17) and is rotatably connected to the box door (21). The box door (21) is welded with a handle (22) on the outside.

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