Three-dimensional culture dish for three-dimensional cell culture
By introducing a stirring rod and a lifting cylinder system into the three-dimensional culture dish, the problem of uneven nutrient distribution was solved, achieving high efficiency and high quality in three-dimensional cell culture and improving the cell expansion and culture success rate.
Patent Information
- Application Number
- CN202423055110.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-11
- Publication Date
- 2025-11-21
- Estimated Expiration
- 2034-12-11
AI Technical Summary
Existing three-dimensional culture dishes result in uneven distribution of nutrients during three-dimensional cell culture, leading to low culture efficiency and poor cell quality.
A three-dimensional culture dish with a stirring rod and a lifting cylinder was designed. The stirring rod is driven by a low-speed motor to rotate at a low speed, and the height of the stirring rod is adjusted by the lifting cylinder to ensure the uniform distribution of nutrients and oxygen and prevent cell precipitation.
This method achieves uniform distribution of nutrients and oxygen, improves cell culture efficiency and quality, reduces cell growth variability, and increases culture success rate.
Smart Images
Figure CN223576513U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the field of three -dimensional culture dish, especially in three -dimensional culture dish for cell three -dimensional culture. BACKGROUND
[0002] Culture dish is a kind of laboratory vessel for microorganism or cell culture, can better simulate the natural environment of cell growth in vivo, cell three-dimensional culture is to make the carrier with three-dimensional structure different material and various different kinds of cells in vitro co-culture, so that cells can migrate, grow in the three-dimensional space structure of carrier, form three-dimensional cell-carrier composite.
[0003] The existing three-dimensional culture dish for cell three-dimensional culture structure is relatively single, in the cell culture process, needs to add multiple nutrients to support the growth of cells and maintain its function, however, cell contact nutrient is insufficient, will make cell nutrition uneven, culture time-consuming, low culture efficiency, and lead to cell growth difference, influence cell quality.
[0004] Therefore, in view of the above existing three-dimensional culture dish cultivation efficiency low problem, a three-dimensional culture dish for cell three-dimensional culture can be designed to promote the uniform distribution of nutrients and oxygen in the culture dish, sufficient stirring, improve the culture efficiency and cell quality of cells, improve the success rate of cell culture. UTILITY MODEL CONTENTS
[0005] In order to overcome the existing three-dimensional culture dish cultivation efficiency low problem.
[0006] The technical scheme of the utility model is as follows: a three-dimensional culture dish for cell three-dimensional culture, including base, still including stirring rod, the upper end of base is provided with culture dish main part, the upper end of culture dish main part is provided with culture dish cover, the right side of the upper end of base is fixedly installed with lifting cylinder, the output end of lifting cylinder is fixedly installed with connecting plate, the upper end of connecting plate is fixedly installed with low-speed motor, the output shaft of low-speed motor is movably penetrated through connecting plate and is fixedly installed with stirring rod, the lower end of the outside of stirring rod is fixedly installed with stirring stick.
[0007] Preferably, the cells are placed in the culture dish main part, the culture dish cover is closed, the low-speed motor drives the stirring rod and the stirring stick to rotate at low speed, the lifting cylinder drives the connecting plate and the stirring rod to move up and down at the same time, the height of the stirring stick is adjusted, the cells and nutrients are fully stirred to ensure uniform distribution and promote cell culture.
[0008] Preferably, the culture dish cover is matched with the culture dish main part, and the outside of the stirring rod is slidably connected with the culture dish cover.
[0009] Preferably, the stirring stick is symmetrically provided with two on the left and right sides of the stirring rod, and the outside of the stirring stick is provided with a silica gel layer.
[0010] As preferred, the left side of the upper end of the culture dish cover is fixedly provided with a feeding pipe, the feeding pipe is communicated with the inside of the culture dish body, and the upper end of the feeding pipe is provided with a movable cover.
[0011] As preferred, the upper end of the culture dish cover is provided with a sliding groove, the inside of the sliding groove is rotatably connected with a sealing cover, and the upper end of the sealing cover is fixedly provided with a poking column.
[0012] As preferred, the lower end of the culture dish cover is provided with a vent, the vent is arranged in a ring shape and has four, and the lower end of the culture dish cover is provided with an air filter membrane.
[0013] As preferred, the upper end of the culture dish body is uniformly provided with anti-skid protrusions, and the lower end of the culture dish body is provided with a rubber layer.
[0014] The culture dish cover has the advantages that:
[0015] The three-dimensional culture dish for three-dimensional culture of cells has the advantages that: the low-speed motor drives the stirring rod and the stirring rod to rotate at low speed, promotes the uniform distribution of nutrients and oxygen in the culture dish, is beneficial to the large expansion and culture of cells, the connecting plate and the stirring rod are driven by the lifting cylinder to move up and down, the height of the stirring rod is adjusted, different doses of cell culture are adapted, the cells and nutrients are fully stirred at different depths, the uniform distribution is ensured, the diffusion of nutrients is promoted, the cell precipitation is prevented, the growth difference caused by uneven nutrition of cells is avoided, the culture efficiency and cell quality of cells are improved, and the success rate of cell culture is improved. BRIEF DESCRIPTION OF DRAWINGS
[0016] Figure 1 The three-dimensional culture dish for three-dimensional culture of cells is shown as a whole three-dimensional structure schematic view;
[0017] Figure 2 The three-dimensional culture dish for three-dimensional culture of cells is shown as a three-dimensional cross-sectional structure schematic view;
[0018] Figure 3 The three-dimensional culture dish for three-dimensional culture of cells is shown as a stirring rod structure schematic view;
[0019] Figure 4 The three-dimensional culture dish for three-dimensional culture of cells is shown as a culture dish cover structure schematic view.
[0020] Explanation of reference signs: 1, base; 2, culture dish main body; 3, culture dish cover; 4, lifting air cylinder; 5, connecting plate; 6, low-speed motor; 7, stirring rod; 8, stirring stick; 9, feeding pipe; 10, sliding groove; 11, sealing cover; 12, pushing column; 13, air vent; 14, air filter membrane; 15, anti-skid protrusion. DETAILED DESCRIPTION
[0021] The utility model is further explained below in combination with the drawings and examples.
[0022] Please refer to Figures 1-4 , the utility model provides a kind of example: a three-dimensional culture dish for cell three-dimensional culture, including base 1, still including stirring rod 7, the upper end of base 1 is provided with culture dish main body 2, the upper end of culture dish main body 2 is provided with culture dish cover 3, lifting air cylinder 4 is fixedly installed on the right side of the upper end of base 1, the output end of lifting air cylinder 4 is fixedly installed with connecting plate 5, the upper end of connecting plate 5 is fixedly installed with low-speed motor 6, the output shaft of low-speed motor 6 is movably penetrated through connecting plate 5 and fixedly installed with stirring rod 7, the lower end of the outside of stirring rod 7 is fixedly installed with stirring stick 8, when using, low-speed motor 6 drives stirring rod 7 and stirring stick 8 to rotate at low speed, promote the uniform distribution of nutrient and oxygen in culture dish, it is favorable to the mass expansion and culture of cell, while lifting air cylinder 4 drives connecting plate 5 and stirring rod 7 to move up and down, by adjusting the height of stirring stick 8, adapt to different dose cell culture, fully stir cell and nutrient at different depth, ensure uniform distribution, promote the diffusion of nutrient, and prevent cell precipitation, avoid cell growth difference due to uneven nutrition, improve the culture efficiency and cell quality of cell, improve the success rate of cell culture.
[0023] Please refer to Figure 1 、 Figure 2 and Figure 3 , in the embodiment, culture dish cover 3 is adapted with culture dish main body 2, the outside of stirring rod 7 is slidably connected with culture dish cover 3, cell is placed in culture dish main body 2, culture dish cover 3 is closed, ensure that interior is not contaminated, two stirring sticks 8 are symmetrically arranged on the left and right sides of stirring rod 7, the outside of stirring stick 8 is provided with silica gel layer, silica gel layer wraps stirring stick 8, effectively reduce the physical damage to cell in stirring process, feeding pipe 9 is fixedly installed on the left side of the upper end of culture dish cover 3, feeding pipe 9 and the inside of culture dish main body 2 are provided with communication, movable cover is installed on the upper end of feeding pipe 9, in the cell culture process, nutrient is added into culture dish main body 2 through movable cover and feeding pipe 9, reduce the pollution caused by cell exposure.
[0024] Please refer to Figure 1 、 Figure 2 and Figure 4In the embodiment, the upper end of the culture dish cover 3 is provided with a sliding groove 10, the inside of the sliding groove 10 is rotatably connected with a sealing cover 11, the upper end of the sealing cover 11 is fixedly installed with a poking column 12, the sealing cover 11 is driven to rotate in the sliding groove 10 through the poking column 12, the lower end of the culture dish cover 3 is provided with air vents 13, the air vents 13 are arranged in a ring shape and there are four of them, the lower end of the culture dish cover 3 is provided with an air filter membrane 14, the outside air passes through the air vents 13 and the air filter membrane 14, the filtered air enters the culture dish, ensuring the oxygen supply in the culture environment, the air filter membrane 14 can effectively filter out bacteria and viruses, ensuring the sterile state of the culture environment, preventing microbial contamination, improving the culture quality of cells, the upper end of the outside of the culture dish main body 2 is uniformly provided with anti-skid protrusions 15, the lower end of the culture dish main body 2 is provided with a rubber layer, the anti-skid protrusions 15 increase friction, preventing slipping when picking up and putting down, the rubber layer improves stability and reduces the impact on the cell culture process.
[0025] In the process of work, the cells are placed in the culture dish main body 2, the culture dish cover 3 is closed, the sealing cover 11 is driven to rotate in the sliding groove 10 by the poking column 12, the outside air passes through the air vents 13 and the air filter membrane 14, the filtered air enters the culture dish, ensuring the oxygen supply in the culture environment, the nutrients are added to the culture dish main body 2 through the movable cover and the feeding pipe 9, the stirring rod 7 and the stirring stick 8 are driven to rotate at low speed by the low-speed motor 6, at the same time, the connecting plate 5 and the stirring rod 7 are driven to move up and down by the lifting cylinder 4, the height of the stirring stick 8 is adjusted to adapt to different doses of cell culture, the cells and nutrients are fully stirred at different depths to ensure uniform distribution, promote the diffusion of nutrients, and prevent cell sedimentation, avoid cell growth differences caused by uneven nutrition, improve the culture efficiency and cell quality of cells, and improve the success rate of cell culture.
[0026] Through the above steps, the use of the lifting cylinder 4 and the stirring rod 7 promotes the uniform distribution of nutrients and oxygen in the culture dish, which is beneficial to the mass expansion and culture of cells, fully stirs the cells and nutrients at different depths to improve the culture efficiency and cell quality of cells, and improves the success rate of cell culture, to solve the problem of low cultivation efficiency of the existing three-dimensional culture dish.
Claims
1. A three-dimensional culture dish for three-dimensional culture of cells comprising a base (1), characterized in that: Also include the stirring rod (7), the upper end of the base (1) is provided with a culture dish body (2), the upper end of the culture dish body (2) is provided with a culture dish cover (3), the right side of the upper end of the base (1) is fixedly installed with a lifting cylinder (4), the output end of the lifting cylinder (4) is fixedly installed with a connecting plate (5), the upper end of the connecting plate (5) is fixedly installed with a low-speed motor (6), the output shaft of the low-speed motor (6) is movably penetrated through the connecting plate (5) and is fixedly installed with a stirring rod (7), the lower end of the outer side of the stirring rod (7) is fixedly installed with a stirring stick (8).
2. The three-dimensional culture dish for cell culture according to claim 1, wherein: The culture dish cover (3) is matched with the culture dish body (2), and the outer side of the stirring rod (7) is slidably connected with the culture dish cover (3).
3. The three-dimensional culture dish for cell culture according to claim 1, wherein: The stirring stick (8) is symmetrically provided with two on the left and right sides of the stirring rod (7), and the outer side of the stirring stick (8) is provided with a silica gel layer.
4. The three-dimensional culture dish for cell culture according to claim 1, wherein: The left side of the upper end of the culture dish cover (3) is fixedly installed with a feeding pipe (9), the feeding pipe (9) and the inside of the culture dish body (2) are communicated, and the upper end of the feeding pipe (9) is installed with a movable cover.
5. The three-dimensional culture dish for cell culture according to claim 1, wherein: The upper end of the culture dish cover (3) is provided with a sliding groove (10), the inside of the sliding groove (10) is rotatably connected with a sealing cover (11), and the upper end of the sealing cover (11) is fixedly installed with a stirring column (12).
6. The three-dimensional culture dish for cell culture according to claim 1, wherein: The lower end of the culture dish cover (3) is provided with a vent (13), the vent (13) is annularly arranged with four, and the lower end of the culture dish cover (3) is provided with an air filter membrane (14).
7. The three-dimensional culture dish for cell culture according to claim 1, wherein: The upper end of the outer side of the culture dish body (2) is uniformly provided with an anti-skid convex (15), and the lower end of the culture dish body (2) is provided with a rubber layer.