High-throughput stability analysis device

Abstract

The utility model relates to the technical field of stability analysis, and provides a high-throughput stability analysis device which comprises a detection platform, a sample tube, a sample tube loading platform, a supporting bottom plate, a driving unit and a lead screw, the driving unit and the lead screw are fixed on the supporting bottom plate through a connecting piece; the sample tube loading platform is fixed at the top of the lead screw and is symmetrically provided with a plurality of sample tube hole sites; the detection platform is installed on the lead screw and comprises a plurality of light path assemblies, and the positions of the light path assemblies correspond to sample tube hole positions; the light path assembly is provided with two light sources with different wavelengths, and each light source is associated with a forward detector and a backward detector. The detection platform moves up and down through the lead screw, and forward and backward optical signals of different positions of a plurality of samples in the vertical direction are detected, so that stability information is obtained. The scheme not only has high-throughput test capability, but also meets the detection requirements of diluted samples and high-concentration and high-turbidity samples, and the arrangement of the double light sources is beneficial to expanding the test range of the device.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of stability analysis test, especially relate to a high throughput stability analysis device. BACKGROUND

[0002] The accurate evaluation of sample stability is very important in food, cosmetics, chemical industry and other industries, and plays an important role in many fields, including the research and development of suspension formula, the research and development of emulsion and foam liquid, the detection and improvement of non-stable suspension, product storage stability prediction, centrifugal acceleration separation process monitoring and the like. However, the existing stability analysis device usually has only one test channel, which limits the test efficiency and is not conducive to large-scale comparative measurement. UTILITY MODEL CONTENT

[0003] The utility model discloses a high throughput stability analysis device, to solve the problem in the background art.

[0004] In order to achieve the above purpose, the utility model provides the following technical scheme:

[0005] A high throughput stability analysis device, comprising a detection platform, a sample tube, a sample tube loading platform, a support bottom plate, a driving unit and a lead screw;

[0006] The driving unit, the lead screw are fixed on the support bottom plate through the connecting piece;

[0007] The sample tube loading platform is fixed on the top of the lead screw, and a plurality of sample tube hole positions are symmetrically arranged on the sample tube loading platform;

[0008] The detection platform is installed on the lead screw, and the detection platform comprises a plurality of optical path assemblies, and the positions of the optical path assemblies correspond to the sample tube hole positions;

[0009] The optical path assembly is provided with two light sources with different wavelengths, and each light source is associated with two detectors in the forward and backward directions.

[0010] The high throughput stability analysis device, preferably, the driving unit comprises a motor, a synchronous pulley and a synchronous belt, and the driving unit is used for driving the lead screw to realize the up-down movement of the detection platform;

[0011] The sample tube loading platform is parallel to the support bottom plate, the sample tube is vertically placed in the sample tube hole position, and a plurality of sample tubes can be placed;

[0012] The sample tube loading platform and the detection platform are both disc-shaped, and the detection platform is parallel to the sample tube loading platform.

[0013] The high throughput stability analysis device, preferably, the sample tube hole position is provided with a sample tube mounting sleeve.

[0014] Preferably, the light path assembly is provided with two light path channels corresponding to the two light sources and the light path channels of the detectors associated with the light sources.

[0015] The light source is inclined by 3-5° to make the light incident on the sample tube, the back detector is 40-42° to the light source, and the forward detector is 135-137° to the back detector.

[0016] The two light sources are divided into light source a with a wavelength of 850 nm and light source b with a wavelength of 470 nm.

[0017] Preferably, the two light sources in the light path assembly are placed at an angle of 90°.

[0018] The forward and back detectors associated with the two light sources are respectively provided with narrow-band optical filters corresponding to the wavelengths of the light sources.

[0019] Preferably, the sample tube installation sleeve is provided with a reflection rod at the lower part, and the material of the reflection rod is polytetrafluoroethylene.

[0020] An analysis method of a high-throughput stability analysis device, comprising the following steps:

[0021] S1: Place the sample tube into the sample tube hole of the sample tube loading platform;

[0022] S2: Drive the detection platform to a specified distance from the reflection rod;

[0023] S3: The control unit controls the driving component to move the detection platform vertically upward according to the step distance set in the software.

[0024] S4: During the vertical movement of the detection platform, the transmission and scattering signals associated with light source 1 are collected and compared with the normal standard of the device to ensure the normality of the light path.

[0025] S5: Collect the transmission and scattering signals of the forward and back detectors associated with light source 1 or light source 2 according to the sample concentration, and save the signal intensity and real-time time of the test to the memory.

[0026] S6: Determine whether the moving position reaches the specified position.

[0027] S7: If it is the specified position, perform the steps described in S5.

[0028] S8: Repeat the operations in steps S3-S7 until the platform moves to the top position of the sample tube set in the software, and stop the test.

[0029] S9: Obtain the signal change trend curve and the change rate value by comparing the test signals at different test times.

[0030] S10: Obtain the stability information of the sample by comparing the change rate values.

[0031] In the preferred scheme of the analysis method of the high-throughput stability analysis device, the specified distance in step S3 is taken as a 0-point reference, and the vertical direction is moved upward, and the distance range of each movement is 10-100 μm.

[0032] In the preferred scheme of the analysis method of the high-throughput stability analysis device, in the scanning process from bottom to top, the air, the reflection rod, and the sample are sequentially passed through, and the light signals are sequentially displayed as the forward detector collects the signal, the backscattering detector has no signal, the forward detector has no signal, the backscattering detector collects the signal, the forward detector and the backscattering detector simultaneously collect the signal. If the signal display is abnormal, the light source and the detector need to be checked and then detected.

[0033] The working principle of the high-throughput stability analysis device is as follows: when a monochromatic light is irradiated to a suspended system sample, the particles in the sample are scattered, and the scattered light is scattered again with multiple particles in the sample in the transmission process, which is multiple light scattering. When the sample concentration is low, a part of the incident light will penetrate the sample to form transmitted light IT; when the sample concentration is high, part of the scattered light will pass through multiple scattering to form backscattering light IBS. The forward and back detectors are placed in front of the light source to detect the transmitted light IT and the backscattering light IBS signals. Since the signals contain information such as sample size, concentration change, and scattering properties, the sample stability information can be derived by detecting the light signals.

[0034] Advantages

[0035] 1. Two light sources with different wavelengths are used, and for each light source, a transmission direction and a backscattering detector are set. When the sample concentration is low, the 850 nm wavelength light has a stronger transmission signal in the medium, and when the sample concentration is high, the 470 nm wavelength light has a stronger scattering signal in the medium. The combination of the two helps to improve and expand the test range of the device.

[0036] 2. For low-concentration samples with high transparency and weak scattering, the transmitted light signal can be used for testing; for samples with high turbidity and strong scattering, the backscattering light can be used for testing, and the highest test concentration can reach 95% v / v.

[0037] 3. Multiple samples can be placed in one device for detection at the same time, with high throughput and high efficiency, which can meet the needs of users for batch detection and convenient sample comparison.

[0038] 4. Non-invasive detection mode, without the need to apply shear and centrifugal force, avoiding interference to the sample. BRIEF DESCRIPTION OF DRAWINGS

[0039] Figure 1 It is a structural schematic diagram of a high-throughput stability analysis device;

[0040] Figure 2 It is a schematic diagram of the initial position of the detection platform;

[0041] Figure 3 It is a top view structural schematic diagram of a high-throughput stability analysis device;

[0042] Figure 4 It is a structural schematic diagram of an optical path assembly;

[0043] Figure 5 It is a schematic diagram of scanning sample signal collection;

[0044] Figure 6 It is a schematic diagram of the change of the instability index (I us ) of multiple samples obtained over time.

[0045] 1, detection platform; 2, sample tube; 3, sample tube loading platform; 4, support bottom plate; 5, lead screw; 6, optical path assembly; 7, motor; 8, synchronous pulley; 9, synchronous belt; 10, sample tube mounting sleeve; 11, reflecting rod; 12, sample tube hole position; 13, light source a; 14, forward detector a; 15, backscattering detector a; 16, light source b; 17, forward detector b; 18, backscattering detector b; 19, narrowband filter a; 20, narrowband filter b; 21, narrowband filter c; 22, narrowband filter d. DETAILED DESCRIPTION

[0046] The utility model will be described below in conjunction with embodiments, obviously, the described embodiments are only a part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the scope of protection of the utility model.

[0047] Please refer to the attached Figures 1-6The application discloses a high-throughput stability analysis device, which comprises a detection platform 1, a sample tube 2, a sample tube loading platform 3, a supporting bottom plate 4, a driving unit and a lead screw 5; the driving unit and the lead screw 5 are fixed on the supporting bottom plate 4 through connecting pieces; the sample tube loading platform 3 is fixed on the top of the lead screw 5, a plurality of sample tube hole positions 12 are symmetrically arranged on the sample tube loading platform 3, and the number of sample tubes to be placed can be selected according to actual conditions; the detection platform 1 is installed on the lead screw 5 and comprises a plurality of optical path assemblies 6, and the positions of the optical path assemblies 6 are correspondingly arranged with the sample tube hole positions 12.

[0048] The optical path assembly 6 is provided with two light sources with different wavelengths, namely a light source a 13 and a light source b 16, and the two light sources are arranged at an angle of 90 degrees; each light source is associated with two forward and backward detectors;

[0049] A narrow-band filter a is arranged in front of the forward detector a 14 associated with the light source a 13, and a narrow-band filter b 20 is arranged in front of the backward detector a 15;

[0050] A narrow-band filter c 21 is arranged in front of the forward detector b 17 associated with the light source b 16, and a narrow-band filter d 22 is arranged in front of the backward detector b 18;

[0051] The light source and the detector are both provided with corresponding light path channels; the light source is inclined by 3-5 degrees so that light is incident on the sample tube 2, the backward detector is arranged at an angle of 40-42 degrees with the light source, and the forward detector is arranged at an angle of 135-137 degrees with the backward detector.

[0052] The sample tube loading platform 3 and the detection platform 1 are disc-shaped, and the two are parallel to the supporting bottom plate 4; the sample tube 2 is vertically arranged in a sample tube mounting sleeve 10, the sample tube mounting sleeve 10 is arranged in the sample tube hole position 12, and a reflection rod 11 made of polytetrafluoroethylene is arranged at the lower part of the sample tube mounting sleeve 10.

[0053] The high-throughput stability detection device further comprises a center circular hole plate, the center of the detection platform 1 is coaxial with the center of the circular hole plate, and the detection platform 1 is arranged in the circular hole of the center circular hole plate in an initial position, so that the subsequent design of a heating space is facilitated.

[0054] The driving unit comprises a motor 7, a synchronous pulley 8 and a synchronous belt 9, and the driving unit drives the lead screw 5 to realize the up-down movement of the detection platform 1.

[0055] The technical scheme sets two light sources, selects appropriate light sources according to the sample concentration, and each light source is associated with two angle measurement light path systems. The transmission direction is used for detecting the transmission signal of the low-concentration high-transmittance sample, and the back direction is used for detecting the scattering signal of the high-turbidity low-transmittance sample. The detection platform of the device is driven by a motor-driven lead screw to move up and down in the Z-axis direction perpendicular to the platform, and step scanning is realized. The signal detection is triggered once each time the platform moves, and the transmission and backscattering light signals at different positions in the vertical direction are used to draw the curves of the transmission and scattering light signals relative to the vertical detection position during the scanning process, and the scanning time is recorded to obtain the information of the sample system at the current time. The scanning process is repeated multiple times, and multiple scattering light signal curves relative to the vertical detection position are drawn, and the corresponding scanning time is recorded. The stability information of the sample is obtained by analyzing the real-time changes of the multiple curve signals.

[0056] A high-throughput stability analysis device according to the technical scheme is used to analyze the stability of six suspension samples, and the specific steps include the following steps:

[0057] S1: Place the sample tube into the sample tube hole of the sample tube loading platform;

[0058] S2: Drive the detection platform to a specified distance from the reflecting rod;

[0059] S3: The control unit controls the driving component to drive the lead screw to move the detection platform upward along the vertical direction according to the step distance set in the software; the step distance is taken as the 0-point reference at a specified distance from the bottom of the reflecting rod, and the moving distance range is 10-100 μm in the vertical direction;

[0060] S4: During the movement of the detection platform in the vertical direction, the transmission and scattering signals associated with light source a are collected and compared with the normal standard of the device to ensure that the light path is normal;

[0061] S5: Collect the transmission and scattering light signals of the forward and backward detectors associated with light source a or light source b according to the sample concentration, and save the signal intensity and real-time test time to the memory;

[0062] S6: Determine whether the moving position reaches the specified position;

[0063] S7: If it is the specified position, perform the steps described in S5;

[0064] S8: Repeat the operations in steps S3-S7 until the platform moves to the top of the sample tube set in the software, and stop testing;

[0065] S9: By comparing the test signals at different test times, a signal change trend curve and a change rate value are obtained;

[0066] S10: By comparing the change rate values, the stability information of the sample can be obtained.

[0067] In the scanning process of the detection platform 4 from bottom to top, the light signal is displayed as follows in turn: air, reflecting rod, sample, forward detector acquires signal, backscattering detector has no signal, backscattering detector acquires signal, forward detector has no signal, forward detector and backscattering detector acquire signals at the same time, and if the signal display is abnormal, the light source and the detector need to be checked and then detected.

[0068] As shown in FIG. 1, the sample is scanned at different times to obtain the backscattering light or forward transmission light signal, and a light signal change curve with sample height is drawn. Figure 5

[0069] As shown in FIG. 2, the signal of each scanning is differentially calculated with the reference signal at the same position, and then the absolute value of the difference is taken and divided by the total sample height H to obtain the instability index I us : Figure 6

[0070] The instability index (I us ) of a plurality of samples is plotted to change with time.

[0071]

[0072] The working principle of a high-throughput stability analysis device is as follows: when a monochromatic light is irradiated to a suspension system sample, the particles in the sample are scattered, and the scattered light is scattered again with multiple particles in the sample in the transmission process, which is multiple light scattering. When the sample concentration is low, a part of the incident light will penetrate the sample to form the transmission light IT; when the sample concentration is high, part of the scattered light will be folded back after multiple scattering to form the backscattering light IBS. The detectors are placed in front of and behind the light source to detect the transmission light IT and the backscattering light IBS signals. Since the signals contain information such as sample size, concentration change, scattering property, etc., the sample stability information can be derived by detecting the light signals.

[0073] Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model should be included in the protection scope of the utility model.​​

Claims

1. A high-throughput stability analysis device, characterized by, The detection platform, the sample tube, the sample tube loading platform, the support base plate, the driving unit and the lead screw are included; The driving unit and the lead screw are fixed on the support base plate through the connecting piece; The sample tube loading platform is fixed on the top of the lead screw, and a plurality of sample tube hole positions are symmetrically arranged on the sample tube loading platform; The detection platform is installed on the lead screw, and the detection platform includes a plurality of optical path assemblies, and the positions of the optical path assemblies correspond to the sample tube hole positions; The optical path assembly is provided with two light sources with different wavelengths, and each light source is associated with two forward and backward detectors; The driving unit includes a motor, a synchronous pulley and a synchronous belt, and is used for driving the lead screw to realize the up-down movement of the detection platform; The sample tube loading platform is parallel to the support base plate, and the sample tube is vertically placed in the sample tube hole position, and a plurality of sample tubes can be placed; The sample tube loading platform and the detection platform are both disc-shaped, and the detection platform is parallel to the sample tube loading platform.

2. A high-throughput stability analysis device according to claim 1, wherein, The sample tube mounting sleeve is arranged in the sample tube hole position.

3. The high-throughput stability analysis device of claim 1, wherein, The optical path assembly is provided with optical path channels corresponding to the two light sources and the optical path channels of the detectors associated with the light sources; The light source is inclined by 3-5 degrees to make the light incident on the sample tube, the backward detector is inclined by 40-42 degrees to the light source, and the forward detector is inclined by 135-137 degrees to the backward detector; The two light sources are light source a and light source b, the wavelength of light source a is 850 nm, and the wavelength of light source b is 470 nm.

4. The high-throughput stability analysis device of claim 1, wherein, The two light sources in the optical path assembly are placed at 90 degrees. The forward and backward detectors associated with the two light sources are respectively provided with narrow-band optical filters corresponding to the wavelengths of the associated light sources.

5. The high-throughput stability analysis device of claim 2, wherein, The lower part of the sample tube mounting sleeve is provided with a reflection rod, and the material of the reflection rod is polytetrafluoroethylene.

6. The high-throughput stability analysis device of claim 1, wherein, During the scanning process of the detection platform from bottom to top, the light signal is displayed as forward detector signal, backward detector no signal, backward detector signal, forward detector and backward detector signal in sequence, and if the signal display is abnormal, the light source and the detector need to be checked and detected again.

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