Optical fiber bundle in-vitro fluorescence mapping equipment

Abstract

The utility model provides optical fiber bundle in-vitro fluorescence mapping equipment, relates to the technical field of experimental equipment, and solves the technical problem in the prior art that heart perfusion failure is possibly caused in a heart transfer process. The device comprises a universal support, an optical fiber conduction piece and a cursor system, the optical fiber conduction piece is connected with the cursor system, the free end of the optical fiber conduction piece is connected with the universal support, and the universal support can adjust the position of the free end of the optical fiber conduction piece. The end face of the free end of the optical fiber conduction piece can be attached to the surface of the experimental heart.

Description

TECHNICAL FIELD

[0001] The utility model relates to experimental equipment technical field especially is related to a kind of optical fiber bundle ex vivo fluorescence marking equipment. BACKGROUND

[0002] There is a method in electrophysiological experiment for taking experimental heart to carry out ex vivo perfusion, after heart is stable, special fluorescent agent (fluorescent agent can emit light of specific wavelength under the irradiation of specific wavelength light) is injected, and heart is transferred to the lens under optical marker system, and is excited by LED lamp irradiation, and optical marker system is photographed to collect fluorescence signal.

[0003] But in this method, the process of transferring heart can cause heart perfusion failure and lead to experimental failure, and the intensity and position of light in experimental process will affect experimental effect;The distance between lens and heart in photographing process will also affect the effect of collecting fluorescence signal. UTILITY MODEL CONTENT

[0004] The utility model discloses a kind of optical fiber bundle ex vivo fluorescence marking equipment, to solve the technical problem that the process of transferring heart can cause heart perfusion failure in prior art.The technical effects produced by preferred technical solutions in many technical solutions provided by the utility model are described in detail as follows.

[0005] To achieve the above object, the utility model provides the following technical scheme:

[0006] The utility model provides a kind of optical fiber bundle ex vivo fluorescence marking equipment, including universal support, optical fiber transmission and optical marker system, wherein the optical fiber transmission is connected with the optical marker system, the free end of the optical fiber transmission is connected with the universal support and the universal support can adjust the position of the free end of the optical fiber transmission, and the end face of the free end of the optical fiber transmission can be attached to the surface of experimental heart.

[0007] Optionally, the universal support includes base, vertical rod, first connecting piece and adjusting piece, the base is connected with the end of the vertical rod, the first connecting piece is provided with through hole and connecting hole, the through hole and the connecting hole are vertically arranged and communicated, the through hole is slidably connected with the vertical rod and the first connecting piece can be lifted and rotated relative to the vertical rod, the adjusting piece is connected with the connecting hole and the end of the adjusting piece can abut on the side wall of the vertical rod, and the first connecting piece is connected with the free end of the optical fiber transmission.

[0008] Optionally, the first connecting piece comprises a first connecting block, a horizontal adjusting rod and a second connecting block, the first connecting block is provided with the through hole, the connecting hole and the movable hole, the through hole, the connecting hole and the movable hole are vertically arranged, the second connecting block is provided with a fixing hole and a mounting hole, one end of the horizontal adjusting rod is connected with the fixing hole, the other end of the horizontal adjusting rod is movably connected with the movable hole, and the free end of the optical fiber conductor is connected with the mounting hole.

[0009] Optionally, the free end of the optical fiber conductor is provided with a fitting part.

[0010] Optionally, the device further comprises a lifting module, the lifting module is connected with the cursor system and can drive the cursor system to move up and down.

[0011] Optionally, the cursor system comprises a body, a cursor lens, a second connecting piece and an LED light source, the body is connected with the lifting module, the body is connected with the cursor lens, the cursor lens is connected with the upper end of the second connecting piece, the end of the optical fiber conductor is connected with the lower end of the second connecting piece, and the LED light source is connected with the side wall of the second connecting piece.

[0012] Optionally, the second connecting piece comprises a lens connecting barrel, a light splitting device, a focusing device and an extension barrel, the lower end of the lens connecting barrel is connected with the upper end of the light splitting device, the upper end of the focusing device is connected with the lower end of the light splitting device, the side end of the light splitting device is connected with the extension barrel, the cursor lens is connected with the lens connecting barrel, the LED light source is connected with the extension barrel, and the end of the optical fiber conductor is connected with the focusing device.

[0013] Optionally, the light splitting device is provided with a dichroic mirror.

[0014] The optical fiber bundle ex vivo fluorescent cursor measurement device can directly image the experimental heart, and the fluorescent response of the experimental heart can be directly conducted to the cursor system, so that the technical problem of possible heart perfusion failure in the process of transferring the heart in the prior art is solved. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings described below are only some of the embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0016] Figure 1 is a structural schematic diagram of the optical fiber bundle ex vivo fluorescent marking equipment provided by the embodiment of the present application;

[0017] Figure 2 is a structural schematic diagram of the optical fiber bundle ex vivo fluorescent marking equipment provided by the embodiment of the present application, which lacks a lifting module;

[0018] Figure 3 is a partial structural schematic diagram of the optical fiber bundle ex vivo fluorescent marking equipment provided by the embodiment of the present application.

[0019] 1, universal support; 11, base; 12, vertical rod; 13, first connecting piece; 131, first connecting block; 132, horizontal adjusting rod; 133, second connecting block; 14, adjusting piece;

[0020] 2, optical fiber transmission element; 21, fitting part;

[0021] 3, experimental heart;

[0022] 4, lifting module;

[0023] 5, main body;

[0024] 6, marking lens;

[0025] 7, second connecting piece; 71, lens connecting barrel; 72, light splitting device; 73, focusing device; 74, extension barrel;

[0026] 8, LED light source. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the present application more clear, the technical scheme of the present application will be described in detail below. Obviously, the described embodiments are only some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of the present application.

[0028] In the description of the utility model, it is necessary to explain that, unless otherwise stated, the meaning of "multiple" is two or more than two;The orientation or position relationship indicated by the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail" and the like is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, therefore it cannot be understood as a limitation on the utility model. In addition, the terms "first", "second", "third" and the like are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0029] In the description of the utility model, it is also necessary to explain that, unless otherwise stated and limited, the terms "mounting", "connection", "connection" should be broadly understood, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected;It can be mechanically connected, or it can be electrically connected;It can be directly connected, or it can be indirectly connected through an intermediate medium. For ordinary skilled persons in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0030] The utility model provides a kind of optical fiber bundle ex vivo fluorescent mark measuring equipment, including universal support 1, optical fiber transmission 2 and light mark system, wherein, optical fiber transmission 2 is connected with light mark system, the free end of optical fiber transmission 2 is connected with universal support 1 and universal support 1 can adjust the position of the free end of optical fiber transmission 2, and the end face of the free end of optical fiber transmission 2 can be attached to the surface of experimental heart 3.The utility model provides a kind of optical fiber bundle ex vivo fluorescent mark measuring equipment, when carrying out experiment, experimental heart does not need to be additionally moved, by adjusting universal support 1, the end face of the free end of optical fiber transmission 2 can be attached to the surface of experimental heart 3, then using optical fiber transmission 2 light transmission imaging principle, make light mark system and experimental heart 3 pass through optical fiber transmission 2 to conduct light, so that excitation light can directly act on the surface of experimental heart 3, and then make light mark system can directly photograph experimental heart 3, and then the fluorescent response of experimental heart 3 can be directly conducted to light mark system, solve the technical problem that heart perfusion failure can be caused in the process of transferring heart in the prior art.

[0031] As an optional implementation, the universal support 1 comprises a base 11, a vertical rod 12, a first connecting piece 13 and an adjusting piece 14, the base 11 is connected with the end of the vertical rod 12, the first connecting piece 13 is provided with a through hole and a connecting hole, the through hole and the connecting hole are vertically arranged and communicated, the through hole is in sliding connection with the vertical rod 12 and the first connecting piece 13 can make lifting and rotating movements relative to the vertical rod 12, thereby the free end of the optical fiber conducting element 2 can also make lifting and rotating movements, the adjusting piece 14 is connected with the connecting hole and the end of the adjusting piece 14 can abut against the side wall of the vertical rod 12, the first connecting piece 13 is connected with the free end of the optical fiber conducting element 2, the adjusting piece 14 is used to limit the movement of the first connecting piece 13, the adjusting piece 14 is in threaded connection with the connecting hole, when the end of the adjusting piece 14 abuts against the side wall of the vertical rod 12, the first connecting piece 13 cannot move relative to the vertical rod 12, when there is a distance between the end of the adjusting piece 14 and the side wall of the vertical rod 12, the first connecting piece 13 can move relative to the vertical rod 12.

[0032] As an optional implementation, the first connecting piece 13 comprises a first connecting block 131, a horizontal adjusting rod 132 and a second connecting block 133, the first connecting block 131 is provided with a through hole, a connecting hole and a movable hole, the through hole, the connecting hole and the movable hole are vertically arranged, the movable hole is not communicated with the through hole, and the through hole and the movable hole are respectively located at two ends of the first connecting block 131, the second connecting block 133 is provided with a fixed hole and a mounting hole, the fixed hole and the mounting hole are respectively located at two ends of the second connecting block 133, one end of the horizontal adjusting rod 132 is connected with the fixed hole, the other end of the horizontal adjusting rod 132 is movably connected with the movable hole, the horizontal adjusting rod 132 can rotate and slide relative to the movable hole, thereby realizing flexible movement of the free end of the optical fiber conducting element 2, and the free end of the optical fiber conducting element 2 is connected with the mounting hole.

[0033] As an optional implementation, the free end of the optical fiber conducting element 2 is provided with a fitting part 21, the fitting part 21 is mounted on the mounting hole, and the end face of the fitting part 21 can be fitted with the surface of the experimental heart 3.

[0034] As an optional implementation, a lifting module 4 is further included, the lifting module 4 is connected with the cursor system and can drive the cursor system to make lifting movement, a moving block of the lifting module 4 is connected with the body 5, and the lifting module 4 can drive the body 5 to make lifting movement.

[0035] As an optional implementation, the cursor system comprises the body 5, the cursor lens 6, the second connecting piece 7 and the LED light source 8, the body 5 is connected with the lifting module 4, the body 5 is connected with the cursor lens 6, the cursor lens 6 is connected with the upper end of the second connecting piece 7, the end of the optical fiber conducting element 2 is connected with the lower end of the second connecting piece 7, the LED light source 8 is connected with the side wall of the second connecting piece 7, and the second connecting piece 7 can realize all-around shielding and discharging of stray light.

[0036] As an optional implementation, the second connecting piece 7 comprises a lens connecting barrel 71, a light splitting device 72, a focusing device 73 and an extension barrel 74, the lower end of the lens connecting barrel 71 is connected with the upper end of the light splitting device 72, the upper end of the focusing device 73 is connected with the lower end of the light splitting device 72, the side end of the light splitting device 72 is connected with the extension barrel 74, the cursor lens 6 is connected with the lens connecting barrel 71, the LED light source 8 is connected with the extension barrel 74, and the end of the optical fiber conducting element 2 is connected with the focusing device 73, and the focusing device 73 is used for adjusting the focal length, so that the end of the optical fiber conducting element 2 and the cursor lens 6 are in focus. The light splitting device 72 can make the excitation light in the LED light source into the optical fiber conducting element 2. A dichroic mirror is arranged in the light splitting device 72.

[0037] The above merely describes the specific implementation of the present application, but the protection scope of the present application is not limited to this. Any skilled person in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A fiber optic bundle ex vivo fluorescence labeling device, characterized in that, It includes a universal support (1), an optical fiber transmission component (2), and a cursor system, wherein, The optical fiber conductor (2) is connected to the cursor system. The free end of the optical fiber conductor (2) is connected to the universal bracket (1), and the universal bracket (1) can adjust the position of the free end of the optical fiber conductor (2). The end face of the free end of the optical fiber conductor (2) can be attached to the surface of the experimental heart (3).

2. The fiber bundle ex vivo fluorescence labeling device according to claim 1, characterized in that, The universal bracket (1) includes a base (11), a vertical rod (12), a first connector (13), and an adjusting member (14). The base (11) is connected to the end of the vertical rod (12). The first connector (13) is provided with a through hole and a connecting hole. The through hole and the connecting hole are vertically arranged and connected. The through hole is slidably connected to the vertical rod (12), and the first connector (13) can move up and down and rotate relative to the vertical rod (12). The adjusting member (14) is connected to the connecting hole, and the end of the adjusting member (14) can abut against the side wall of the vertical rod (12). The first connector (13) is connected to the free end of the optical fiber conductor (2).

3. The fiber bundle in vitro fluorescence labeling device according to claim 2, characterized in that, The first connector (13) includes a first connecting block (131), a horizontal adjusting rod (132), and a second connecting block (133). The first connecting block (131) is provided with the through hole, the connecting hole, and the movable hole. The through hole, the connecting hole, and the movable hole are arranged perpendicularly. The second connecting block (133) is provided with a fixing hole and a mounting hole. One end of the horizontal adjusting rod (132) is connected to the fixing hole, and the other end of the horizontal adjusting rod (132) is movably connected to the movable hole. The free end of the optical fiber conductor (2) is connected to the mounting hole.

4. The fiber optic bundle ex vivo fluorescence labeling device according to claim 1, characterized in that, The free end of the optical fiber conductor (2) is provided with a bonding part (21).

5. The fiber bundle ex vivo fluorescence labeling device according to claim 1, characterized in that, It also includes a lifting module (4), which is connected to the cursor system and can drive the cursor system to perform lifting and lowering movements.

6. The fiber bundle in vitro fluorescence labeling device according to claim 5, characterized in that, The cursor system includes a body (5), a cursor lens (6), a second connector (7), and an LED light source (8). The body (5) is connected to the lifting module (4), the body (5) is connected to the cursor lens (6), the cursor lens (6) is connected to the upper end of the second connector (7), the end of the fiber optic conductor (2) is connected to the lower end of the second connector (7), and the LED light source (8) is connected to the side wall of the second connector (7).

7. The fiber bundle in vitro fluorescence labeling device according to claim 6, characterized in that, The second connector (7) includes a lens connecting tube (71), a beam splitter (72), a focusing device (73), and an extension tube (74). The lower end of the lens connecting tube (71) is connected to the upper end of the beam splitter (72), the upper end of the focusing device (73) is connected to the lower end of the beam splitter (72), the side end of the beam splitter (72) is connected to the extension tube (74), the cursor lens (6) is connected to the lens connecting tube (71), the LED light source (8) is connected to the extension tube (74), and the end of the fiber optic conductor (2) is connected to the focusing device (73).

8. The fiber bundle in vitro fluorescence labeling device according to claim 7, characterized in that, The spectral splitter (72) is equipped with a dichroic mirror.

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