Culture medium bottle for preparing Columbia blood agar plate
By introducing a fixed motor and a limiting rod into the culture medium bottle, quantitative transfer of the culture medium solution was achieved, solving the problem of uneven dispensing of the culture medium solution and ensuring the accuracy and consistency of experimental results.
Patent Information
- Application Number
- CN202422879236.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-26
- Publication Date
- 2025-11-25
- Estimated Expiration
- 2034-11-26
AI Technical Summary
Existing Columbia blood agar plate culture bottles cannot achieve quantitative dispensing of culture medium, resulting in inconsistent microbial growth conditions in different culture dishes, which affects the accuracy and consistency of experimental results.
A fixed motor drives a fixed threaded rod to rotate. The limiting rod limits the movement of the threaded cylinder, which in turn pushes the fixed block and rubber stopper to move. The culture medium solution enters the fixed tube through the suction tube and the one-way valve. The fixed motor controls the transfer of the culture medium solution to ensure that the amount of culture medium solution in each petri dish is the same.
This ensured that the amount of culture medium in each petri dish was the same, maintaining the accuracy and consistency of the experimental results.
Smart Images

Figure CN223592711U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of culture medium bottle technology, specifically a culture medium bottle for preparing Columbia blood agar plates. Background Technology
[0002] Culture media bottles are commonly used laboratory containers in the fields of biology, medicine, and chemistry for storing and transporting serum, cell culture media, culture media, etc. The main components of Columbia blood agar plates include peptone, beef extract, yeast extract, soluble starch, sodium chloride, agar, and sterile defibrinated sheep blood. These components are precisely formulated, autoclaved, and a measured amount of animal blood is added at the filling temperature before being poured into disposable plastic plates.
[0003] When preparing Columbia blood agar plates, the culture medium solution stored in the culture medium bottle needs to be dispensed into petri dishes. Currently, the culture medium bottles for Columbia blood agar plates are usually filled manually by pouring the culture medium solution from the bottle into the petri dishes. This method cannot measure the quantity of culture medium, resulting in different amounts of culture medium solution in each petri dish. This may lead to differences in the growth conditions of microorganisms in different petri dishes, thus affecting the accuracy and consistency of experimental results. Utility Model Content
[0004] This invention provides a culture medium bottle for preparing Columbia blood agar plates. A fixed motor drives a fixed threaded rod to rotate, and a limiting rod limits the movement of the threaded cylinder, which in turn moves the fixed block and rubber stopper. The culture medium solution inside the culture medium bottle enters the fixed tube through the suction tube and the first one-way valve. Then, the fixed motor drives the fixed block and rubber stopper to push the culture medium solution out of the fixed tube through the second one-way valve and the transfer tube. This allows the culture medium solution to be transferred to the petri dishes, ensuring that the amount of culture medium solution in each petri dish is the same, thereby maintaining the accuracy and consistency of experimental results.
[0005] To achieve the above objectives, this utility model provides the following technical solution: a culture medium bottle for preparing Columbia blood agar plates, comprising: a culture medium bottle body; a transfer mechanism, the transfer mechanism including a fixed tube, a mounting sleeve, an aspiration component, and a transfer component, the mounting sleeve being fixedly sleeved on the outer surface of the fixed tube, the mounting sleeve being threadedly connected to the top of the culture medium bottle body, the aspiration component and the transfer component both being disposed on the fixed tube; and a quantitative mechanism, the quantitative mechanism including a pushing component, a moving component, and a quantitative component, the quantitative component being disposed on the fixed tube, the pushing component and the moving component both being disposed on the aspiration component.
[0006] Furthermore, the suction component includes a first one-way valve and a suction tube, one end of the first one-way valve is fixedly connected to the bottom of the fixed tube, and one end of the suction tube is fixedly connected to the other end of the first one-way valve.
[0007] Furthermore, the transmission component includes a second one-way valve and a transmission pipe. One end of the second one-way valve is fixedly connected to the bottom of the fixed pipe, and one end of the transmission pipe is fixedly connected to the other end of the second one-way valve, with the other end of the transmission pipe passing through the fixed pipe.
[0008] Furthermore, the quantitative component includes a movable plug, two electric telescopic rods, and a limiting plate. The bottom of the movable plug is threaded to the top of the fixed tube, one end of each of the two electric telescopic rods is fixedly connected to the bottom of the movable plug, and the top of the limiting plate is fixedly connected to the extended ends of the two electric telescopic rods. The limiting plate is slidably embedded between the inner walls of the fixed tube.
[0009] Furthermore, the pushing component includes a fixed motor, a fixed threaded rod, two limiting rods, and a threaded cylinder. The top end of the fixed threaded rod rotatably penetrates the lower inner wall of the movable plug. The fixed motor is fixedly connected to one side inner wall of the movable plug, and the output end of the fixed motor is fixedly connected to one end of the fixed threaded rod. The top ends of the two limiting rods are fixedly connected to the bottom of the movable plug. One end of the threaded cylinder penetrates through a limiting plate, and the threaded cylinder is threadedly sleeved on the outer surface of the fixed threaded rod. The threaded cylinder is slidably sleeved on the outer surface of the two limiting rods.
[0010] Furthermore, the moving component includes a fixed block and a rubber plug. The top of the fixed block is fixedly connected to the bottom end of the threaded cylinder, and the rubber plug is fixedly sleeved on the outer surface of the fixed block and slidably sleeved between the inner walls of the fixed tube.
[0011] This invention provides a culture medium bottle for preparing Columbia blood agar plates. It offers the following advantages: The culture medium bottle, driven by a fixed motor, rotates a fixed threaded rod. A limiting rod then moves the threaded cylinder, pushing a fixed block and a rubber stopper. The culture medium solution inside the bottle enters the fixed tube through a suction tube and a first one-way valve. The fixed motor then drives the fixed block and rubber stopper to push the solution out of the fixed tube through a second one-way valve and a transfer tube, transferring the solution to the petri dishes. This ensures that the amount of culture medium solution in each petri dish is the same, thus maintaining the accuracy and consistency of experimental results. Attached Figure Description
[0012] Figure 1 This is a frontal perspective view of the present invention;
[0013] Figure 2 This is a frontal three-dimensional sectional view of the present invention;
[0014] Figure 3 This is a frontal perspective half-sectional view of the present invention;
[0015] Figure 4 This is a side-view perspective half-sectional view of the present invention.
[0016] In the diagram: 1. Culture medium bottle body; 2. Quantitative mechanism; 201. Movable plug; 202. Fixed motor; 203. Fixed threaded rod; 204. Limiting rod; 205. Threaded cylinder; 206. Fixing block; 207. Rubber stopper; 208. Electric telescopic rod; 209. Limiting plate; 3. Transmission mechanism; 301. Fixed tube; 302. Mounting sleeve; 303. First one-way valve; 304. Suction tube; 305. Second one-way valve; 306. Transmission tube. Detailed Implementation
[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.
[0018] Please see Figure 1-4 This utility model provides a technical solution: a culture medium bottle for preparing Columbia blood agar plates, comprising: a culture medium bottle body 1; a transfer mechanism 3, which includes a fixed tube 301, a mounting sleeve 302, an aspiration component, and a transfer component, wherein the mounting sleeve 302 is fixedly sleeved on the outer surface of the fixed tube 301 and threadedly connected to the top of the culture medium bottle body 1, and the aspiration component and the transfer component are both disposed on the fixed tube 301; and a quantitative mechanism 2, which includes a pushing component, a moving component, and a quantitative component, wherein the quantitative component is disposed on the fixed tube 301, and the pushing component and the moving component are both disposed on the aspiration component.
[0019] In this embodiment: the transmission mechanism 3 can transmit culture medium solution, the fixed tube 301 is a graduated fixed tube 301, the mounting sleeve 302 is set so that the fixed tube 301 is connected to the culture medium bottle body 1, the suction component is used to suction the culture medium solution in the culture medium bottle body 1, the transmission component is set to transmit the culture medium solution in the fixed tube 301, the quantitative mechanism 2 can be adjusted according to the culture medium solution to be suctioned, the adjustment, the pushing component can adjust the position of the moving component, and the quantitative component is set for quantitative adjustment.
[0020] Specifically, the suction component includes a first one-way valve 303 and a suction tube 304. One end of the first one-way valve 303 is fixedly connected to the bottom of the fixed tube 301, and one end of the suction tube 304 is fixedly connected to the other end of the first one-way valve 303.
[0021] In this embodiment: the first one-way valve 303 allows the culture medium solution to pass through in the culture medium bottle body 1.
[0022] Specifically, the transmission component includes a second one-way valve 305 and a transmission pipe 306. One end of the second one-way valve 305 is fixedly connected to the bottom of the fixed pipe 301, and one end of the transmission pipe 306 is fixedly connected to the other end of the second one-way valve 305, with the other end of the transmission pipe 306 passing through the fixed pipe 301.
[0023] In this embodiment: the second one-way valve 305 is installed to transfer the culture medium solution during the transfer of the culture medium solution.
[0024] Specifically, the metering component includes a movable plug 201, two electric telescopic rods 208, and a limiting plate 209. The bottom of the movable plug 201 is threaded to the top of the fixed tube 301. One end of each of the two electric telescopic rods 208 is fixedly connected to the bottom of the movable plug 201. The top of the limiting plate 209 is fixedly connected to the extended ends of the two electric telescopic rods 208, and the limiting plate 209 is slidably embedded between the inner walls of the fixed tube 301.
[0025] In this embodiment: two electric telescopic rods 208 can push the limiting plate 209 to move. The position of the limiting plate 209 can be adjusted according to the amount of culture medium solution to be absorbed. The principle and structure of the electric telescopic rods 208 are common knowledge to those skilled in the art and will not be described in detail here. The model can be selected according to the actual use.
[0026] Specifically, the pushing component includes a fixed motor 202, a fixed threaded rod 203, two limiting rods 204, and a threaded cylinder 205. The top end of the fixed threaded rod 203 rotatably passes through the lower inner wall of the movable plug 201. The fixed motor 202 is fixedly connected to one side inner wall of the movable plug 201, and the output end of the fixed motor 202 is fixedly connected to one end of the fixed threaded rod 203. The top ends of the two limiting rods 204 are fixedly connected to the bottom of the movable plug 201. One end of the threaded cylinder 205 passes through the limiting plate 209, and the threaded cylinder 205 is threadedly sleeved on the outer surface of the fixed threaded rod 203. The threaded cylinder 205 is slidably sleeved on the outer surface of the two limiting rods 204.
[0027] In this embodiment: the fixed motor 202 drives the fixed threaded rod 203 to rotate, and the threaded cylinder 205 pushes the moving part to move by the limiting rod 204. The fixed motor 202 can rotate in both directions. The principle and structure of the fixed motor 202 are common knowledge to those skilled in the art and will not be described in detail here. Its model can be selected according to the actual use.
[0028] Specifically, the moving parts include a fixed block 206 and a rubber plug 207. The top of the fixed block 206 is fixedly connected to the bottom of the threaded cylinder 205, and the rubber plug 207 is fixedly sleeved on the outer surface of the fixed block 206 and slidably sleeved between the inner walls of the fixed tube 301.
[0029] In this embodiment: the pushing component can drive the fixing block 206 and the rubber stopper 207 to rise or fall, which is used to draw and transfer the culture medium solution.
[0030] In use, when assembling the culture medium bottle body 1, the transfer mechanism 3 is inserted into the culture medium bottle body 1, and then the transfer mechanism 3 is fixed to the culture medium bottle body 1 by the mounting sleeve 302. Then, the metering mechanism 2 is inserted into the fixing tube 301, and then the metering mechanism 2 is fixed in the fixing tube 301 by the movable plug 201. When taking culture medium solution, the limit plate 209 is moved to the appropriate position by the electric telescopic rod 208, and the fixing motor 202 is turned on. The fixing motor 202 drives the fixing threaded rod 203 to rotate. By the limit of the limit rod 204, the threaded cylinder 205 pushes the fixing block 206 and the rubber stopper 207 to move. The culture medium solution in the culture medium bottle body 1 enters the fixing tube 301 through the suction tube 304 and the first one-way valve 303. Then, by the fixing motor 202, the fixing block 206 and the rubber stopper 207 push the culture medium solution, so that the culture medium solution is transferred out of the fixing tube 301 through the second one-way valve 305 and the transfer tube 306, and the culture medium solution can be transferred to the petri dish.
[0031] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.
Claims
1. A culture medium bottle for preparing Columbia blood agar plates, characterized in that, include: Culture medium bottle body (1); The transmission mechanism (3) includes a fixed tube (301), a mounting sleeve (302), a suction component and a transmission component. The mounting sleeve (302) is fixedly sleeved on the outer surface of the fixed tube (301). The mounting sleeve (302) is threaded to the top of the culture medium bottle body (1). The suction component and the transmission component are both disposed on the fixed tube (301). The quantitative mechanism (2) includes a pushing component, a moving component and a quantitative component. The quantitative component is disposed on a fixed tube (301), and the pushing component and the moving component are both disposed on a suction component.
2. The culture medium bottle for preparing Columbia blood agar plates according to claim 1, characterized in that: The suction component includes a first one-way valve (303) and a suction tube (304). One end of the first one-way valve (303) is fixedly connected to the bottom of the fixed tube (301), and one end of the suction tube (304) is fixedly connected to the other end of the first one-way valve (303).
3. A culture medium bottle for preparing Columbia blood agar plates according to claim 2, characterized in that: The transmission component includes a second one-way valve (305) and a transmission pipe (306). One end of the second one-way valve (305) is fixedly connected to the bottom of the fixed pipe (301), and one end of the transmission pipe (306) is fixedly connected to the other end of the second one-way valve (305). The other end of the transmission pipe (306) passes through the fixed pipe (301).
4. A culture medium bottle for preparing Columbia blood agar plates according to claim 3, characterized in that: The quantitative component includes a movable plug (201), two electric telescopic rods (208), and a limiting plate (209). The bottom of the movable plug (201) is threaded to the top of the fixed tube (301). One end of each of the two electric telescopic rods (208) is fixedly connected to the bottom of the movable plug (201). The top of the limiting plate (209) is fixedly connected to the extended ends of the two electric telescopic rods (208), and the limiting plate (209) is slidably embedded between the inner walls of the fixed tube (301).
5. A culture medium bottle for preparing Columbia blood agar plates according to claim 4, characterized in that: The pushing component includes a fixed motor (202), a fixed threaded rod (203), two limiting rods (204), and a threaded cylinder (205). The top end of the fixed threaded rod (203) rotatably passes through the lower inner wall of the movable plug (201). The fixed motor (202) is fixedly connected to one side inner wall of the movable plug (201), and the output end of the fixed motor (202) is fixedly connected to one end of the fixed threaded rod (203). The top ends of the two limiting rods (204) are fixedly connected to the bottom of the movable plug (201). One end of the threaded cylinder (205) passes through the limiting plate (209), and the threaded cylinder (205) is threaded onto the outer surface of the fixed threaded rod (203). The threaded cylinder (205) is slidably sleeved on the outer surface of the two limiting rods (204).
6. A culture medium bottle for preparing Columbia blood agar plates according to claim 5, characterized in that: The moving part includes a fixed block (206) and a rubber plug (207). The top of the fixed block (206) is fixedly connected to the bottom end of the threaded cylinder (205). The rubber plug (207) is fixedly sleeved on the outer surface of the fixed block (206) and slidably sleeved between the inner walls of the fixed tube (301).