Microbial cell wall breaking machine

By combining the pulverizing and crushing components, microbial cells are pre-crushed and then crushed, solving the problem of incomplete cell wall breaking in a single operation. This achieves efficient and uniform cell wall breaking, reducing production complexity and costs.

CN223592716UActive Publication Date: 2025-11-25JIANGXI SHANXING BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202520199922.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-02-08
Publication Date
2025-11-25
Estimated Expiration
2035-02-08

AI Technical Summary

Technical Problem

Existing microbial cell disruption devices can only perform single-cycle disruption, which may result in incomplete disruption of some cells, increasing production complexity and cost.

Method used

Cells are pre-disrupted using a pulverizing component, followed by secondary disruption using a crushing component, and further disruption using high-pressure jetting and a high-speed rotating needle tube.

Benefits of technology

It achieves complete cell wall disruption of microorganisms, reduces subsequent processing steps, lowers production costs and equipment investment, and improves cell wall disruption efficiency and uniformity.

✦ Generated by Eureka AI based on patent content.

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  • Figure CN223592716U_ABST
    Figure CN223592716U_ABST
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Abstract

The utility model discloses a microbial cell wall breaking machine which comprises a supporting table, a smashing box is arranged at the top of the supporting table, a smashing assembly for conducting pre-smashing treatment on microbial cells is arranged in the smashing box, and the bottom of the smashing box is communicated with a discharging pipe. Through the arrangement of the crushing assembly and the crushing assembly, the crushing assembly in the crushing box performs pre-crushing treatment on microbial cells, the cells are preliminarily crushed into small particles, and a foundation is laid for fine wall breaking of the subsequent crushing assembly, so that the wall breaking efficiency is integrally improved; the crushing assembly in the crushing box further treats the preliminarily crushed cells to ensure that cell walls are fully destroyed, more substances in the cells are released and the wall breaking effect is enhanced, and secondary crushing design enables crushing treatment of the microbial cells to be completed in one step, so that subsequent treatment steps and equipment investment are reduced, and the production efficiency is improved. And the production cost is further saved.
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Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of microorganism, specifically is a microbial cell wall breaking machine. BACKGROUND

[0002] Microorganisms are a collective term for a class of organisms, including bacteria, viruses, fungi and the like, covering a large number of beneficial and harmful species, and widely involving food, medicine, industry and agriculture, environmental protection, sports and many other fields, and closely related to human beings.

[0003] The prior art provides a wall breaking device for extracting biological cells (publication number CN220812443U), which comprises a rotating cavity arranged in the interior of the base, a gear disc arranged in the interior of the rotating cavity, and a fine filter screen frame arranged in the interior of the material cylinder; a lifting seat arranged above the base, a mounting rack arranged above the lifting seat, a first electric telescopic rod arranged in the interior of the lifting seat, a cover plate arranged below the mounting rack and fixedly connected with the mounting rack; a lifting plate arranged in the interior of the mounting rack, a second motor arranged above the lifting plate and connected with the lifting plate through screws, a wall breaking knife paddle arranged below the cover plate and connected at one end with the output end of the second motor, and the wall breaking knife paddle is movably connected with the cover plate. The wall breaking knife paddle of the wall breaking device can be adjusted in lifting during use, so that the wall breaking knife paddle can fully contact with the biological cells in the fine filter screen frame, and the wall breaking effect of the biological cells is improved.

[0004] Based on the above patent search, the above patent can adjust the wall breaking knife paddle in lifting during use to process the cells, but only single wall breaking treatment can be performed on the cells, and single wall breaking treatment may not completely destroy the cell walls of all cells, resulting in uneven wall breaking effect of part of the cells, which may require additional processing steps to further break the cells, increasing the complexity and cost of production. Therefore, we need to propose a microbial cell wall breaking machine. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a microbial cell wall breaking machine, through the setting of the smashing assembly and the breaking assembly, the smashing assembly carries out the preliminary breaking treatment to the microbial cells, and the cells are initially broken into smaller particles, laying a foundation for the fine wall breaking of the subsequent breaking assembly, and then the breaking assembly further breaks the cells subjected to the preliminary breaking, ensuring that the cell walls are fully destroyed and more intracellular substances are released, and enhancing the wall breaking effect, so as to solve the problems in the above background art.

[0006] To achieve the above object, the utility model provides the following technical scheme:

[0007] A microbial cell wall breaking machine, comprising a support table, a crushing box is arranged on the top of the support table, a crushing assembly for pre-crushing treatment of microbial cells is arranged in the crushing box, and a discharge pipe is communicated with the bottom of the crushing box;

[0008] A crushing box is arranged on the top of the support table, and a crushing assembly for secondary crushing of microbial cells is arranged in the crushing box.

[0009] Preferably, the crushing assembly comprises a high-pressure pump fixedly installed on the top of the support table, an input end of the high-pressure pump is communicated with one end of the discharge pipe, an output end of the high-pressure pump is communicated with a conveying pipe, the other end of the conveying pipe penetrates the crushing box and is communicated with a communication disc, one end of the communication disc is communicated with a plurality of needle-shaped pipes, a first rotating rod is rotatably installed in the crushing box, one end of the first rotating rod is installed with a rotating disc, and one side of the crushing box is provided with a driving member for driving the rotating disc to rotate at high speed.

[0010] Preferably, one side of the communication disc is fixedly connected with one side in the crushing box, and the plurality of needle-shaped pipes are arranged in annular array.

[0011] Preferably, the driving member comprises a first motor fixedly installed on one side of the crushing box, an output end of the first motor is fixedly connected with a second rotating rod through a shaft coupling, an outer side of the second rotating rod is fixedly installed with a first gear, an outer side of the first rotating rod is fixedly installed with a second gear, and the first gear and the second gear are engaged.

[0012] Preferably, a support frame is fixedly installed on one side of the crushing box, and one end of the first rotating rod and one end of the second rotating rod are rotatably installed on one side in the support frame.

[0013] Preferably, the crushing assembly comprises a second motor fixedly installed on the top of the crushing box, an output end of the second motor is fixedly connected with a third rotating rod through a shaft coupling, an outer side of the third rotating rod is provided with a plurality of crushing knives, and a bottom end of the third rotating rod is rotatably installed on the inner bottom of the crushing box.

[0014] Preferably, the bottom of the crushing box is provided with a flow guide seat, and the flow guide seat is arranged in an inclined manner.

[0015] Compared with the prior art, the microbial cell wall breaking machine has the advantages that:

[0016] The utility model discloses a crushing assembly and the setting of the crushing assembly, and the crushing assembly in the crushing box carries out the preliminary crushing treatment to the microbial cell, and the cell is primarily crushed into smaller particles, and the fine wall breaking of the subsequent crushing assembly is laid a foundation, thereby improving the wall breaking efficiency as a whole, and then the crushing assembly in the crushing box carries out further treatment to the cell after the preliminary crushing, ensures that the cell wall is fully destroyed, releases more intracellular substances, enhances the wall breaking effect, and the secondary crushing design makes the crushing treatment of the microbial cell be in place in one step, reduces the subsequent processing steps and the investment of equipment, and further saves the production cost. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is the structural schematic diagram of the utility model;

[0018] Figure 2 It is the sectional view schematic diagram of the utility model crushing box;

[0019] Figure 3 It is the structural schematic diagram of the utility model crushing assembly;

[0020] Figure 4 It is the sectional view schematic diagram of the utility model crushing box.

[0021] In the drawing: 1, support platform, 2, crushing box, 3, crushing assembly, 31, second motor, 32, third rotary rod, 33, crushing knife, 4, discharge pipe, 5, crushing box, 6, crushing assembly, 61, high-pressure pump, 62, conveying pipe, 63, communication disc, 64, needle-shaped tube, 65, first rotary rod, 66, rotating disc, 67, driving part, 671, first motor, 672, second rotary rod, 673, first gear, 674, second gear, 7, support frame, 8, flow guide seat. DETAILED DESCRIPTION

[0022] The technical scheme in the embodiments of the utility model will be described clearly and completely below in conjunction with the drawings in the embodiments of the utility model, and obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative labor belong to the scope of protection of the utility model.

[0023] Please refer to Figures 1-4 The utility model provides a technical scheme:

[0024] A microbial cell wall breaking machine, comprising a support platform 1, the top of the support platform 1 is provided with a crushing box 2, the inside of the crushing box 2 is provided with a crushing assembly 3 for preliminary crushing treatment of microbial cells, and the bottom of the crushing box 2 is communicated with a discharge pipe 4.

[0025] The top of the support table 1 is provided with a crushing box 5, and the inside of the crushing box 5 is provided with a crushing assembly 6 for secondary crushing of microbial cells.

[0026] The crushing assembly 6 comprises a high-pressure pump 61 fixedly installed on the top of the support table 1, an input end of the high-pressure pump 61 is communicated with one end of the discharge pipe 4, an output end of the high-pressure pump 61 is communicated with a conveying pipe 62, the other end of the conveying pipe 62 penetrates the crushing box and is communicated with a communication disc 63, one end of the communication disc 63 is communicated with a plurality of needle-shaped pipes 64, a first rotating rod 65 is rotatably installed in the inside of the crushing box 5, one end of the first rotating rod 65 is installed with a rotating disc 66, one side of the crushing box 5 is provided with a driving member 67 for driving the rotating disc 66 to rotate at high speed.

[0027] Through the setting of the crushing assembly 6, the high-pressure pump 61 can generate high pressure, and the pre-crushed microbial cells are conveyed to the communication disc 63 through the conveying pipe 62, and then sprayed on the rotating disc 66 in the form of high speed and high pressure by the plurality of needle-shaped pipes 64, and such high-pressure spraying can rapidly crush the microbial cell wall and improve the crushing efficiency, and at the same time, the driving member 67 drives the first rotating rod 65 and the rotating disc 66 to rotate at high speed, and the high-pressure sprayed microbial cells produce strong collision and friction, which further enhances the crushing effect.

[0028] One side of the communication disc 63 is fixedly connected with one side in the inside of the crushing box 5, and the plurality of needle-shaped pipes 64 are arranged in annular array.

[0029] Through the fixed connection of the communication disc 63 with the inside of the crushing box 5, the stability and accuracy of the needle-shaped pipes 64 are ensured, and such design prevents the needle-shaped pipes 64 from being displaced due to vibration or external force, so as to ensure that the high-pressure water flow or air flow can be stably and accurately sprayed on the microbial cells in the crushing box 5, and the plurality of needle-shaped pipes 64 are arranged in annular array, so that the high-pressure water flow or air flow can be uniformly distributed on the rotating disc 66, avoiding the situation that some cells are excessively crushed while other cells are not crushed, and improving the uniformity and quality of crushing.

[0030] The driving member 67 comprises a first motor 671 fixedly installed on one side of the crushing box 5, an output end of the first motor 671 is fixedly connected with a second rotating rod 672 through a shaft coupling, the outer side of the second rotating rod 672 is fixedly installed with a first gear 673, the outer side of the first rotating rod 65 is fixedly installed with a second gear 674, and the first gear 673 and the second gear 674 are engaged.

[0031] Through the setting of the driving member 67, the first motor 671 drives the second rotating rod 672 and the first gear 673 to rotate, the second gear 674 engaged with the first gear 673 drives the first rotating rod 65 and the rotating disc 66 to rotate at high speed, and stable transmission and flexible adjustment enable the rotating disc 66 to crush the microbial cells at the best rotating speed and force, thereby improving the crushing efficiency and quality.

[0032] One side of the crushing box 5 is fixedly provided with a support frame 7, one end of the first rotating rod 65 and one end of the second rotating rod 672 are rotatably installed on one side inside the support frame 7.

[0033] The support frame 7 provides stable support points for the first rotating rod 65 and the second rotating rod 672, ensuring that they can rotate smoothly during the crushing process and are not prone to shaking or deviation, thereby improving the accuracy and efficiency of the crushing.

[0034] The crushing assembly 3 comprises a second motor 31 fixedly installed on the top of the crushing box 2, the output end of the second motor 31 is fixedly connected with a third rotating rod 32 through a shaft coupling, the outer side of the third rotating rod 32 is provided with a plurality of crushing knives 33, and the bottom end of the third rotating rod 32 is rotatably installed on the inner bottom of the crushing box 2.

[0035] Through the setting of the crushing assembly 3, the second motor 31 drives the third rotating rod 32 and the crushing knives 33 to rotate at high speed, cuts and impacts the microbial cells put into the crushing box 2, realizes preliminary crushing, and lays a foundation for fine wall breaking of the subsequent crushing assembly 6.

[0036] The bottom of the crushing box 5 is provided with a flow guide seat 8, and the flow guide seat 8 is inclined.

[0037] Through the setting of the flow guide seat 8, the inclined flow guide seat 8 can utilize the action of gravity to make the crushed microbial cells more smoothly discharge from the crushing box 5, reduce the accumulation and blockage in the box, and improve the discharge efficiency.

[0038] Working principle: when the utility model is used, the microbial cells enter the crushing box 2 from the feeding pipe, the second motor 31 is started, the second motor 31 drives the third rotating rod 32 and the crushing knives 33 to rotate at high speed, cuts and impacts the microbial cells put into the crushing box 2, realizes preliminary crushing, then the high-pressure pump 61 and the first motor 671 are started, the first motor 671 drives the second rotating rod 672 and the first gear 673 to rotate, the second gear 674 meshing with the first gear 673 drives the first rotating rod 65 and the rotating disc 66 to rotate at high speed, the high-pressure pump 61 draws the preliminarily crushed cell liquid from the discharge pipe 4, then the cell liquid is conveyed to the communicating disc 63 in the crushing box 5 through the conveying pipe 62, the communicating disc 63 is communicated with a plurality of needle-shaped pipes 64 arranged in an annular array, the needle-shaped pipes 64 spray the cell liquid in the form of high pressure and high speed onto the rotating disc 66 rotating at high speed, so as to collide and rub with the microbial cells, further enhance the crushing effect on the microbial cells, and thus complete the crushing work of the microbial cells.

[0039] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present application, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A microbial cell wall disruptor, comprising a support platform (1), characterized in that: The top of the support platform (1) is provided with a crushing box (2), the inside of the crushing box (2) is provided with a crushing component (3) for pre-crushing microbial cells, and the bottom of the crushing box (2) is connected to a discharge pipe (4). The top of the support platform (1) is provided with a crushing box (5), and the inside of the crushing box (5) is provided with a crushing component (6) for secondary crushing of microbial cells.

2. The microbial cell wall disruptor according to claim 1, characterized in that: The crushing assembly (6) includes a high-pressure pump (61) fixedly installed on the top of the support platform (1). The input end of the high-pressure pump (61) is connected to one end of the discharge pipe (4). The output end of the high-pressure pump (61) is connected to a conveying pipe (62). The other end of the conveying pipe (62) passes through the crushing box and is connected to a connecting plate (63). One end of the connecting plate (63) is connected to multiple sets of needle tubes (64). A first rotating rod (65) is rotatably installed inside the crushing box (5). A turntable (66) is installed at one end of the first rotating rod (65). A drive component (67) is provided on one side of the crushing box (5) to drive the turntable (66) to rotate at high speed.

3. A microbial cell wall disruptor according to claim 2, characterized in that: One side of the connecting plate (63) is fixedly connected to one side inside the crushing box (5), and multiple sets of needle tubes (64) are arranged in a ring array.

4. A microbial cell wall disruptor according to claim 2, characterized in that: The drive unit (67) includes a first motor (671) fixedly installed on one side of the crushing box (5). The output end of the first motor (671) is fixedly connected to a second rotating rod (672) via a coupling. A first gear (673) is fixedly installed on the outer side of the second rotating rod (672). A second gear (674) is fixedly installed on the outer side of the first rotating rod (65). The first gear (673) and the second gear (674) mesh.

5. A microbial cell wall disruptor according to claim 4, characterized in that: A support frame (7) is fixedly installed on one side of the crushing box (5), and one end of the first rotating rod (65) and one end of the second rotating rod (672) are rotatably installed on one side inside the support frame (7).

6. A microbial cell wall disruptor according to claim 1, characterized in that: The crushing assembly (3) includes a second motor (31) fixedly installed on the top of the crushing box (2). The output end of the second motor (31) is fixedly connected to a third rotating rod (32) via a coupling. Multiple sets of crushing blades (33) are provided on the outer side of the third rotating rod (32). The bottom end of the third rotating rod (32) is rotatably installed on the inner bottom of the crushing box (2).

7. A microbial cell wall disruptor according to claim 1, characterized in that: The bottom of the crushing box (5) is provided with a flow guide seat (8), which is inclined.

Citation Information

Patent Citations

  • Wall breaking device for biological cell extraction

    CN220812443U