Rapid detection device for number of clostridium butyricum

Abstract

The utility model discloses a rapid detection device for the number of clostridium butyricum, and belongs to the technical field of clostridium butyricum detection. The clostridium butyricum number rapid detection device comprises a constant temperature incubator, a housing is arranged above the constant temperature incubator, the housing is rotatably connected with the constant temperature incubator through a hinge, one end of the constant temperature incubator is provided with a touch table, and the other end of the constant temperature incubator is provided with an electric heating module. In order to solve the problems of low thallus biomass of a clostridium butyricum preparation under large-scale production due to the fact that a large amount of organic nitrogen is required to be supplied as a nitrogen source during cultivation of the existing clostridium butyricum and the whole cultivation period is relatively long, the clostridium butyricum is put into a culture dish, then the culture dish is put into a limiting seat groove in the surface of a shaking table, a housing is closed, and the clostridium butyricum is put into the limiting seat groove; the fan pipe groove is connected with the vacuum pump, air in the box body is pumped away through the vacuum pump, and then the culture temperature is adjusted through the electric heating module, so that spores of the clostridium butyricum are completely mature after being cultured for 18 hours.

Description

TECHNICAL FIELD

[0001] The utility model relates to butyric acid clostridium detection technical field, specifically to a kind of butyric acid clostridium rapid detection device. BACKGROUND

[0002] Butyric acid clostridium is also named tyrocidin, butyric acid clostridium, butyric acid bacteria, can be digested, absorbed and play immunoregulatory effect to nutrient substance, is important function of animal intestinal tract, therefore, it is necessary to carry out intestinal health care, maintain intestinal health, especially after prohibition, it is very necessary, at present, there are many products about intestinal health care on market, mainly acidifier, probiotic, medium short chain fatty acid etc., and Chinese herbal medicine and its extract, essential oil, oligosaccharide, fiber, enzyme preparation, glutamine etc. Product also plays an important role.

[0003] Existing butyric acid clostridium needs a lot of organic nitrogen as nitrogen source supply when cultivating, and the whole cultivation cycle is long, resulting in low butyric acid clostridium preparation biomass under large-scale production. UTILITY MODEL CONTENT

[0004] The utility model discloses a kind of butyric acid clostridium rapid detection device, butyric acid clostridium is placed in petri dish, then petri dish is placed in the limiting seat groove on the surface of shaking table, close shell cover, fan pipe groove is connected with vacuum pump, the air inside box is extracted by vacuum pump, then the culture temperature is adjusted by electric heating module, so that butyric acid clostridium is completely mature after 18h cultivation, can solve the problems in prior art.

[0005] To achieve the above object, the utility model provides the following technical scheme: a kind of butyric acid clostridium rapid detection device, including constant-temperature incubator, the upper portion of constant-temperature incubator is provided with shell cover, shell cover is rotationally connected with constant-temperature incubator by hinge, one end of constant-temperature incubator is provided with touch table, the other end of constant-temperature incubator is provided with electric heating module, one side of constant-temperature incubator top is provided with fan pipe groove, the inside of constant-temperature incubator is provided with shaking table, the upper portion of shaking table is provided with a plurality of petri dishes.

[0006] Further, the bottom of the shaking table is provided with a motor, and the outer surface of the shaking table is provided with a limiting seat groove.

[0007] Further, one side of the inside of the constant-temperature incubator is provided with a metal heat-conducting plate, and the metal heat-conducting plate is connected with the electric heating module through a clamping groove.

[0008] Further, the upper portion of the metal heat-conducting plate is provided with a drying air bellow, and the drying air bellow is connected with the constant-temperature incubator through bolts.

[0009] Further, the culture dish is arranged as a conical structure, and the bottom of the culture dish is provided with a support connected with the culture dish through a clamping groove.

[0010] Further, the culture dish is connected with the limiting seat groove through the support, and the outer surface of the support is provided with LED lamp beads.

[0011] Compared with the prior art, the culture dish has the advantages that:

[0012] 1. The butyric acid clostridium is put into the culture dish, then additives such as flour, fish meal and yeast powder are added as carbon source and nitrogen source, then the culture dish is placed in the limiting seat groove on the surface of the shaking table, the shell cover is closed, the fan pipe groove is connected with the vacuum pump, the air in the box body is extracted through the vacuum pump, the culture temperature is adjusted through the electric heating module, the growth environment of the strain during the whole culture process is maintained, the strain can be quickly divided, the spores of the butyric acid clostridium are completely matured after 18h culture, and the strain cultivation period is greatly shortened, so that the cost consumption caused by the long period is reduced.

[0013] 2. The culture dish is arranged as a conical structure, the conical culture dish can avoid liquid pouring out during shaking, the culture dish is combined with the limiting seat groove on the surface of the shaking table through the support at the bottom, the culture dish is locked, the LED lamp beads at the bottom of the culture dish can provide light source from the bottom, the background plate formed by the strong light source is used to distinguish the strain and the nutrient solution, so that the colony distribution in the culture dish and the approximate quantity of the strain are displayed, the culture condition of the strain can be directly understood from the outside of the box body without taking out the culture dish for observation, the rapid determination is realized, and the influence on the environment in the box body is effectively avoided. BRIEF DESCRIPTION OF DRAWINGS

[0014] Fig. 1 It is the overall front view of the utility model;

[0015] Fig. 2 It is the overall expanded structure schematic view of the utility model;

[0016] Fig. 3 It is the culture dish structure schematic view of the utility model.

[0017] In the drawing: 1, constant temperature incubator; 2, shell cover; 3, culture dish; 4, drying air box; 101, touch table; 102, fan pipe groove; 103, electric heating module; 104, shaking table; 105, limiting seat groove; 106, gravity sensing module; 1031, metal heat conduction plate; 301, support; 302, LED lamp bead. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the drawings in the embodiments of the present utility model. Obviously, the described embodiments are only part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present utility model.

[0019] In order to solve the problem that the existing Clostridium butyricum needs a large amount of organic nitrogen as a nitrogen source during cultivation, and the whole cultivation cycle is long, resulting in low biomass of Clostridium butyricum preparation in large-scale production, please refer to Figs. 1-3 The present utility model provides the following solutions:

[0020] A Clostridium butyricum number rapid detection device, including constant temperature incubator 1, the top of constant temperature incubator 1 is provided with shell cover 2, shell cover 2 and constant temperature incubator 1 are connected through hinge rotation, one end of constant temperature incubator 1 is provided with touch table 101, the other end of constant temperature incubator 1 is provided with electric heating module 103, one side of the top of constant temperature incubator 1 is provided with fan pipe groove 102, the inside of constant temperature incubator 1 is provided with shaking table 104, the top of shaking table 104 is provided with multiple petri dishes 3, Clostridium butyricum is placed in petri dish 3, then flour, fish meal and yeast powder and other additives are added as carbon source and nitrogen source, then petri dish 3 is placed in limiting seat groove 105 on the surface of shaking table 104, shell cover 2 is closed, fan pipe groove 102 is connected with vacuum pump, the air in the box body is extracted through vacuum pump, the culture temperature is adjusted through electric heating module 103, the content of protein, polypeptide and free amino acid is rich, which not only provides nutrition for the rapid growth of Clostridium butyricum, but also provides nutrition for the rapid production of Clostridium butyricum spores in the later stage, so that the Clostridium butyricum spores are completely mature after 18h culture;

[0021] The bottom of shaking table 104 is provided with motor, the outer surface of shaking table 104 is provided with limiting seat groove 105, the inside of limiting seat groove 105 is provided with gravity sensing module 106, one side of the inside of constant temperature incubator 1 is provided with metal heat conduction plate 1031, metal heat conduction plate 1031 is connected with electric heating module 103 through clamping groove, the top of metal heat conduction plate 1031 is provided with drying air bellow 4, drying air bellow 4 is connected with constant temperature incubator 1 through bolt;

[0022] The petri dish 3 is arranged in a conical structure, the conical petri dish 3 can avoid the liquid pouring out in the shaking process, the bottom of the petri dish 3 is provided with a support 301, the support 301 is connected with the petri dish 3 through a clamping groove, the petri dish 3 is connected with the limiting seat groove 105 through the support 301, the outer surface of the support 301 is provided with an LED lamp bead 302, the petri dish 3 is combined with the limiting seat groove 105 on the surface of the shaking table 104 through the bottom support 301, the locking of the petri dish 3 is completed, the LED lamp bead 302 can intuitively feed back the strain quantity and distribution in the petri dish 3 to the staff, so that the rapid determination is realized.

[0023] Working principle, put clostridium butyricum into the petri dish 3, then add additives such as flour, fish meal and yeast powder as carbon source and nitrogen source, then place the petri dish 3 in the limiting seat groove 105 on the surface of the shaking table 104, close the shell cover 2, the fan pipe groove 102 is connected with the vacuum pump, the air in the box body is removed through the vacuum pump, and the culture temperature is adjusted through the electric heating module 103, the content of protein, polypeptide and free amino acid is rich, which not only provides nutrition for the rapid growth of clostridium butyricum, but also provides nutrition for the rapid production of clostridium butyricum spores in the later stage, so that the clostridium butyricum spores are completely matured after 18h culture.

[0024] It should be noted that in this paper, the relationship terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between the entities or operations. Moreover, the terms "include", "contain" or any other variants thereof are intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements not only includes those elements, but also includes other elements not explicitly listed or inherent to such process, method, article or equipment.

[0025] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A rapid detection device for Clostridium butyricum, characterized in that, Including constant temperature incubator (1), the upper portion of constant temperature incubator (1) is provided with a casing (2), the casing (2) is rotatably connected with constant temperature incubator (1) through a hinge, one end of constant temperature incubator (1) is provided with a touch table (101), the other end of constant temperature incubator (1) is provided with an electric heating module (103), one side of the top of constant temperature incubator (1) is provided with a fan pipe groove (102), the inside of constant temperature incubator (1) is provided with a shaking table (104), the upper portion of shaking table (104) is provided with a plurality of culture dishes (3).

2. The rapid detection device for Clostridium butyricum according to claim 1, characterized in that: The bottom of the shaking table (104) is provided with a motor, the outer surface of the shaking table (104) is provided with a limit seat groove (105), the inside of the limit seat groove (105) is provided with a gravity sensing module (106).

3. The rapid detection device for Clostridium butyricum according to claim 1, characterized in that: One side of the inside of the constant temperature incubator (1) is provided with a metal heat conduction plate (1031), the metal heat conduction plate (1031) is connected with the electric heating module (103) through a clamping groove.

4. The rapid detection device for Clostridium butyricum according to claim 3, characterized in that: The upper portion of the metal heat conduction plate (1031) is provided with a drying air bellow (4), the drying air bellow (4) is connected with the constant temperature incubator (1) through a bolt.

5. The rapid detection device for Clostridium butyricum according to claim 2, characterized in that: The culture dish (3) is provided as a conical structure, the bottom of the culture dish (3) is provided with a support (301), the support (301) is connected with the culture dish (3) through a clamping groove.

6. The rapid detection device for Clostridium butyricum according to claim 5, characterized in that: The culture dish (3) is connected with the limit seat groove (105) through the support (301), the outer surface of the support (301) is provided with an LED lamp bead (302).

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