Polypeptide extraction device

Abstract

The utility model discloses a polypeptide extraction device. The polypeptide extraction device comprises a base, a reaction part and a magnetic component, a bearing platform is arranged on the base; the reaction part is arranged on the bearing platform, and a sample solution and a magnetic unit are accommodated in the reaction part; the magnetic component is arranged on the base and selectively attracts the magnetic unit. According to the polypeptide extraction device disclosed by the utility model, in the polypeptide extraction process, the magnetic unit or the magnetic unit-polypeptide polymer can be selectively and timely aggregated by utilizing magnetic force according to the operation purpose, so that the extraction process can be efficiently carried out, the mechanization of the operation is improved, and the operation is simple and orderly.

Description

TECHNICAL FIELD

[0001] The utility model relates to polypeptide extraction technical field especially is a kind of polypeptide extraction device. BACKGROUND

[0002] In the related technology of polypeptidomics research, magnetic bead method is generally used to extract polypeptides. The magnetic bead method extracts polypeptides by adsorbing free polypeptide molecules in the sample with magnetic beads to form a magnetic bead-polypeptide complex, washing away impurities such as proteins that are not adsorbed under the action of a magnetic field, obtaining the magnetic bead-polypeptide complex, and then separating the magnetic beads from the polypeptide molecules using a washing solution to obtain pure polypeptides. In the process of extracting polypeptides by magnetic beads, in order to make the solid-liquid separation more thorough, a magnet is needed to gather the magnetic bead-polypeptide complex. In related technologies, the magnet is fixedly arranged. However, due to the continuous action of the magnetic field, the magnetic beads are always in an enriched state and cannot be fully mixed and contacted with the solution. The magnet can be detachably arranged, but it needs to be manually operated frequently, which is inconvenient and inefficient. SUMMARY

[0003] The utility model aims at at least one of the technical problems existing in prior art. To this end, one object of the utility model is to provide a polypeptide extraction device that can mechanically and selectively apply a magnetic field to a solution containing magnetic units, simplify the extraction process and improve the polypeptide extraction efficiency.

[0004] The utility model embodiment provides a kind of polypeptide extraction device, polypeptide extraction device includes: pedestal, reaction part and magnetic component;Pedestal is provided with bearing platform;Reaction part is arranged in bearing platform, and reaction part is suitable for containing sample solution and magnetic unit in it;Magnetic component is arranged in pedestal and can selectively adsorb magnetic unit.

[0005] According to the polypeptide extraction device of the utility model, the magnetic component and the reaction part are arranged in the pedestal, and the magnetic component can selectively apply adsorption force to the magnetic unit in the reaction part. Therefore, during the polypeptide extraction process, the magnetic beads or magnetic bead-polypeptide aggregates can be gathered in time according to the operation purpose using magnetic force, which facilitates efficient extraction process and improves the mechanical operation, making the operation simple and orderly.

[0006] According to some embodiments of the utility model, the magnetic component is movably arranged in the pedestal to selectively approach or move away from the magnetic unit to adsorb or reduce the adsorption force on the magnetic unit.

[0007] According to some embodiments of the utility model, the polypeptide extraction device further includes a guide rail and a bracket. The guide rail is arranged in the pedestal. The bracket is movably arranged in the guide rail to selectively approach or move away from the bearing platform. The magnetic component is arranged in the bracket and is suitable for moving synchronously with the bracket.

[0008] According to some embodiments of the present application, the guide rail is arranged on the bearing platform and extends on the surface of the bearing platform or is perpendicular to the bearing platform.

[0009] According to some embodiments of the present application, the magnetic component is configured as an electromagnet, and the electromagnet is adapted to selectively generate or eliminate the adsorption force on the magnetic unit.

[0010] According to some embodiments of the present application, the polypeptide extraction device further comprises a collection part and a liquid discharge device, the collection part is arranged on the bearing platform, and a collection cavity is formed in the collection part to collect the solution discharged from the reaction part; the liquid discharge device is arranged on the base, and the liquid discharge device is adapted to drive the solution in the reaction part to be discharged into the collection cavity.

[0011] According to some embodiments of the present application, the liquid discharge device is arranged on the bracket and selectively extends into the reaction part to change the air pressure in the reaction part to control the liquid discharge in the reaction part.

[0012] According to some embodiments of the present application, the liquid discharge device is provided with an exhaust outlet adapted to discharge air into the reaction part to change the gas pressure in the reaction part.

[0013] According to some embodiments of the present application, the reaction part comprises a reaction tube and a hole plate, the reaction tube is configured as a plurality of and is arranged at intervals on the bearing platform, one end of each reaction tube away from the bearing platform is formed with a matching part, and the other end of the reaction tube is provided with a liquid discharge port; a plurality of first holes and a plurality of second holes are formed on the hole plate, each first hole is one-to-one corresponding to the plurality of reaction tube matching parts and is adapted to be passed through by the liquid discharge device to enter the reaction tube, and the second hole is adapted to be passed through by the magnetic component.

[0014] According to some embodiments of the present application, the liquid discharge device is configured as an exhaust needle, the exhaust needle is configured as a plurality of corresponding to the reaction tube, each exhaust needle is adapted to pass through the first hole and extend into the reaction tube; the magnetic component is configured as a plurality of corresponding to the exhaust needle, each magnetic component is arranged in parallel with the exhaust needle and moves to the outer wall of the reaction tube through the corresponding second hole.

[0015] Additional aspects and advantages of the present application will be given in part in the following description, some of which will become apparent from the following description, or will be learned by practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0016] The above and / or additional aspects and advantages of the present application will become apparent and more readily appreciated from the following description of embodiments, taken in conjunction with the accompanying drawings, in which:

[0017] Figure 1 is a polypeptide extraction device structure schematic diagram according to some embodiments of the present application;

[0018] Figure 2is a structural schematic view of a hole plate according to some embodiments of the present application;

[0019] Figure 3 is a structural schematic view of a reaction tube according to some embodiments of the present application;

[0020] Figure 4 is a schematic view of a cooperation state of a reaction tube and a liquid discharge device, and a magnetic component according to some embodiments of the present application;

[0021] Figure 5 is a structural schematic view of a collection portion according to some embodiments of the present application;

[0022] Figure 6 is an assembly schematic view of a liquid discharge device, a magnetic component, and a mounting portion according to some embodiments of the present application.

[0023] Reference signs:

[0024] Base 10; bearing platform 11;

[0025] Reaction portion 20; hole plate 21; first hole 211; second hole 212; reaction tube 22; liquid discharge port 221; barrier 222;

[0026] Magnetic component 30;

[0027] Collection portion 40; collection tube 41;

[0028] Liquid discharge device 50; coarse adjustment switch 51; fine adjustment switch 52;

[0029] Support 60; mounting portion 61; guide rail 62; support adjustment switch 63;

[0030] First sliding portion 71; second sliding portion 72;

[0031] Magnetic unit 80. DETAILED DESCRIPTION

[0032] Embodiments of the present application are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference signs represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the accompanying drawings are exemplary, only for explaining the present application, and cannot be understood as limiting the present application.

[0033] The polypeptide extraction device according to the embodiments of the present application is described below with reference to Figures 1-5 The polypeptide extraction device according to the embodiments of the present application is described below with reference to

[0034] The utility model discloses an embodiment of polypeptide extraction device, polypeptide extraction device includes: base 10, reaction part 20 and magnetic component 30, base 10 is provided with the bearing platform 11, reaction part 20 sets up in bearing platform 11, and reaction part 20 is suitable for containing sample solution and magnetic unit 80 in, and magnetic component 30 sets up in base 10 and can selectively adsorb magnetic unit 80.

[0035] Need to explain is, the main steps of polypeptide extraction of magnetic bead method include: preparation contains polypeptide molecule sample solution, add magnetic unit 80 such as magnetic bead and incubate and make magnetic bead adsorb polypeptide molecule and form magnetic bead-polypeptide polymer, wash and obtain solid magnetic bead-polypeptide polymer, elution makes magnetic bead and polypeptide molecule dispersion, gather magnetic bead and collect polypeptide solution, wherein, when washing solid magnetic bead-polypeptide polymer, need to utilize magnet and make the polymer gather so that the solution containing impurities is fully discharged, when collecting polypeptide solution, need to utilize magnet and make magnetic bead gather so that the polypeptide solution after extraction can be fully discharged.

[0036] According to the polypeptide extraction device of the utility model embodiment, sample solution containing magnetic unit 80 is placed in reaction part 20, and extraction is carried out in reaction part 20, reaction part 20 is arranged on bearing platform 11, and bearing platform 11 and magnetic component 30 are arranged on base 10, so the magnetic field of magnetic component 30 can selectively cover reaction part 20, and then selectively exert magnetic force on magnetic unit 80 in reaction part 20 to adsorb magnetic unit 80.

[0037] According to the polypeptide extraction device of the utility model, since magnetic component 30 and reaction part 20 are arranged on base 10, and magnetic component 30 selectively exerts adsorption force on magnetic unit 80 in reaction part 20, therefore, in the polypeptide extraction process, magnetic unit 80 or magnetic unit 80-polypeptide polymer can be selectively gathered in time according to the operation purpose by using magnetic force, which facilitates efficient extraction process, improves the mechanization degree of operation, and makes the operation simple and orderly.

[0038] According to some embodiments of the present application, the magnetic component 30 is movably arranged on the base 10 to selectively approach the magnetic unit 80 and adsorb the magnetic unit 80 or move away from the magnetic unit 80 to reduce the adsorption force on the magnetic unit 80. In the present embodiment, the magnetic component 30 is movably arranged on the base 10, so that the distance between the magnetic component 30 and the reaction part 20 is variable. When the magnetic component 30 approaches the reaction part 20, the adsorption force of the magnetic component 30 on the magnetic unit 80 in the reaction part 20 increases. When the magnetic component 30 moves away from the reaction part 20, the adsorption force of the magnetic component 30 on the magnetic unit 80 in the reaction part 20 decreases. When the magnetic component 30 moves away from the reaction part 20 to a certain distance, the adsorption force of the magnetic component 30 on the magnetic unit 80 in the reaction part 20 is equal to zero. The magnetic unit 80 can be dispersed in the solution and fully contact with the solution. When the magnetic component 30 approaches the side wall of the reaction part 20, the adsorption force of the magnetic component 30 on the magnetic unit 80 in the reaction part 20 is strong and stable, and the magnetic unit 80 can be gathered on the side wall of the reaction part 20 and separated from the solution.

[0039] According to some embodiments of the present application, the polypeptide extraction device further comprises a guide rail 62 and a bracket 60. The guide rail 62 is arranged on the base 10. The bracket 60 is movably arranged on the guide rail 62 to selectively approach or move away from the bearing platform 11. The magnetic component 30 is arranged on the bracket 60 and is adapted to move synchronously with the bracket 60. In the present embodiment, the magnetic component 30 is arranged on the bracket 60, the bracket 60 is movably arranged on the guide rail 62, and the guide rail 62 is arranged on the base 10. The magnetic component 30 can move relative to the base 10, so as to change the relative position of the magnetic component 30 in the reaction part 20, and further change the adsorption force of the magnetic component 30 on the magnetic unit 80. In the present embodiment, the movement of the bracket 60 changes the adsorption force of the magnetic component 30 on the magnetic unit 80, which can make the operation visualized and reduce the possibility of misoperation. At the same time, it can be applied to synchronous control of multiple magnetic components 30 and simplify the operation process. In some embodiments, as shown in FIG. 6, part of the guide rail 62 is accommodated in the base 10, and the other part is connected with the bracket 60. The two parts of the guide rail 62 are slidably connected to realize the movement of the bracket 60 relative to the base 10. Further, the base 10 is provided with a bracket adjusting switch 63 to adjust the movement amount of the bracket 60, so that the operation process is more refined. Figure 1

[0040] ​According to some embodiments of the present application, the guide rail 62 is arranged on the bearing platform 11 and extends on the surface of the bearing platform 11 or the guide rail 62 is perpendicular to the bearing platform 11. In some embodiments, the guide rail 62 is arranged on the bearing platform 11 and extends on the surface of the bearing platform 11, so that the bracket 60 can move along the surface of the bearing platform 11 relative to the base 10, so that the magnetic component 30 can move in a direction perpendicular to the side wall of the reaction part 20 to change the size of the adsorption force on the magnetic unit 80. In some embodiments, the guide rail 62 is arranged perpendicular to the bearing platform 11, as shown in the figure, so that the bracket 60 can move in a direction perpendicular to the bearing platform 11 relative to the bearing platform 11, so that the magnetic component 30 moves in a direction parallel to the side wall of the reaction part 20 to change the size of the adsorption force on the magnetic unit 80. Figure 1

[0041] It should be noted that when the magnetic component 30 moves in a direction parallel to the side wall of the reaction part 20, if the upper part of the reaction part 20 is open, the magnetic unit 80 may be relatively moved with the reaction part 20 under the action of the continuous adsorption force of the magnetic component 30, or even follow the magnetic component 30 to move upward and escape from the reaction part 20.

[0042] To solve the above problems, according to some embodiments of the present application, the magnetic component 30 is configured as an electromagnet, and the electromagnet is suitable for selectively generating or eliminating the adsorption force on the magnetic unit 80. This embodiment realizes the control of the adsorption force on the magnetic unit 80 by controlling whether the electromagnet is powered on or not, and can quickly disconnect the adsorption force without the need for the magnetic unit 80 or the magnetic unit 80-polypeptide polymer aggregation, so that the magnetic unit 80 or the magnetic unit 80-polypeptide polymer is dispersed in the solution. This embodiment can improve the application range of the magnet by arranging the electromagnet, so that the magnet is more suitable for application in the polypeptide extraction process, and the adaptability of the device is improved.

[0043] In some embodiments, the magnetic component 30 can be configured as an electromagnet fixedly arranged on the base 10 or the bearing platform 11, and only power control is used to selectively apply a magnetic force to the magnetic unit 80 in the reaction part 20 to adsorb the magnetic unit 80. This embodiment can further simplify the structure and simplify the operation process.

[0044] It should be noted that in the process of polypeptide extraction, especially in the process of obtaining the magnetic unit 80-polypeptide complex and obtaining the extracted polypeptide solution, multiple solid-liquid separations are required, and the polypeptide extraction process is carried out in the reaction part 20, so that the intermediate products of the reaction need to be collected and processed in time.

[0045] ​According to some embodiments of the present application, the polypeptide extraction device further comprises a collection part 40 and a liquid discharge device 50. The collection part 40 is arranged on the bearing platform 11, and a collection cavity is formed in the collection part 40 to collect the solution discharged from the reaction part 20. The liquid discharge device 50 is arranged on the base 10, and the liquid discharge device 50 is adapted to drive the solution in the reaction part 20 to be discharged into the collection cavity. In this embodiment, the waste liquid during the reaction and the polypeptide solution after extraction can be discharged from the reaction part 20 and collected by the collection part 40, as shown in FIG. 4. During collection, the magnetic part 30 is used to adsorb the magnetic unit 80 or the magnetic unit 80-polypeptide complex to the side wall, and the solution in the reaction part 20 is discharged through the liquid discharge device 50. Figure 5

[0046] In some embodiments, the reaction part 20 is detachably arranged on the collection part 40, and the collection part 40 is detachably arranged on the bearing platform 11. The detachable collection part 40 can realize replacement of the collection part 40. During extraction, the waste liquid collection part 40 and the sample collection part 40 are replaced, which facilitates separate collection and processing of the waste liquid and the polypeptide solution after extraction, and avoids mutual contamination. Further, the reaction part 20 is detachably arranged above the collection part 40. The reaction part 20 is formed with a liquid discharge port 221, and the solution in the reaction part 20 can directly fall into the collection part 40 through the liquid discharge port 221, without the need for additional backflow or conveying structure, so that the structure is more compact and convenient to operate and replace. Further, in order to prevent the solution from flowing out of the liquid discharge port 221 automatically, as shown in FIG. 5, the reaction part 20 is provided with a barrier 222 arranged at the liquid discharge port 221 and adapted to generate a liquid discharge resistance to block the solution from flowing out of the liquid discharge port 221. In some embodiments, the barrier 222 can be configured as a combination of a precision screen and a filler, and there is a filtrate gap to realize the closed liquid discharge port 221 by using atmospheric pressure. Figure 3 4

[0047] In some embodiments, in order to facilitate replacement of the collection part 40, as shown in FIG. 6, the bearing platform 11 is provided with a first sliding part 71, and the base 10 is provided with a second sliding part 72. The first sliding part 71 and the second sliding part 72 cooperate to realize movement of the bearing platform 11 relative to the base 10, thereby realizing movement of the reaction part 20 and the collection part 40 relative to the base 10, which facilitates avoiding structural interference to detach and replace the collection part 40, and also facilitates adding solution during the extraction process. Figure 1

[0048] ​​​​According to some embodiments of the present application, the liquid discharge device 50 is arranged on the support 60 and can selectively extend into the reaction part 20 to change the air pressure in the reaction part 20 to control the liquid discharge in the reaction part 20. The liquid discharge device 50 of the present embodiment can change the air pressure in the reaction part 20, and when the air pressure in the reaction part 20 reaches a certain value, the reaction part 20 can generate an opening for the solution to flow out to discharge the solution. Changing the air pressure to achieve solution discharge can avoid affecting the properties of the solution and simplify the structure of the reaction part 20, without the need to set up a valve or other control mechanism.

[0049] According to some embodiments of the present application, the liquid discharge device 50 is provided with an exhaust outlet suitable for discharging air into the reaction part 20 to change the air pressure in the reaction part 20. The liquid discharge device 50 of the present embodiment can deliver gas into the reaction part 20 to change the air pressure in the reaction part 20, and when the air pressure in the reaction part 20 reaches a certain value, the reaction part 20 can generate an opening or channel for the solution to flow out to discharge the solution. In the present embodiment, changing the air pressure by inputting gas into the reaction part 20 can avoid contaminating the solution and ensure the reliability of the polypeptide extraction device. Further, the liquid discharge device 50 of the present embodiment can be used in cooperation with the blocking piece 222, the blocking piece 222 uses atmospheric pressure to close the liquid discharge port 221, and the liquid discharge device 50 increases the air pressure in the reaction part 20 to offset the resistance of the blocking piece 222 to achieve solution discharge.

[0050] According to some embodiments of the present application, the reaction part 20 includes: a reaction tube 22 and a hole plate 21, the reaction tube 22 is constructed as a plurality and is arranged at intervals on the bearing platform 11, one end of each reaction tube 22 away from the bearing platform 11 is formed with a fitting part, and the other end of the reaction tube 22 is provided with a liquid discharge port 221; the hole plate 21 is formed with a plurality of first holes 211 and a plurality of second holes 212, each first hole 211 is one-to-one corresponding to the plurality of reaction tube 22 fitting parts and is suitable for the liquid discharge device 50 to enter into the reaction tube 22, and the second hole 212 is suitable for the magnetic component 30 to pass through.

[0051] In the present embodiment, as shown in Figure 2 The reaction tube 22 is arranged in the first hole 211 through the fitting part, which can realize the arrangement and installation of a plurality of reaction tubes 22, simultaneously perform a plurality of extraction processes, and improve the throughput and efficiency of extraction; at the same time, the hole plate 21 is provided with the second hole 212, the magnetic component 30 passes through and extends into the second hole 212, which can realize that the magnetic component 30 is close to the side wall of the corresponding reaction tube 22, and facilitates the magnetic component 30 to apply adsorption force to the magnetic unit 80 in the air. Further, the first hole 211 and the second hole 212 are arranged at intervals and one-to-one corresponding, which can realize that each reaction tube 22 is close to one magnetic component 30, and ensure that the magnetic force received by the magnetic unit 80 in each reaction tube 22 is uniform and stable. Further, asFigure 5 As shown, the collecting part 40 is provided with a plurality of collecting tubes 41 corresponding to the reaction tubes 22, so as to receive the solution discharged from the reaction tubes 22.

[0052] According to some embodiments of the present application, the liquid discharge device 50 is configured as an exhaust needle, and the exhaust needle is configured as a plurality of exhaust needles corresponding to the reaction tubes 22, and each exhaust needle is adapted to penetrate into the reaction tube 22 through the first hole 211; the magnetic component 30 is configured as a plurality of magnetic components corresponding to the exhaust needles, and each magnetic component 30 is arranged in parallel with the exhaust needle and is moved to the outer side wall of the reaction tube 22 through the corresponding second hole 212.

[0053] In the present embodiment, as shown, Figures 1-4 the liquid discharge device 50 is configured as an exhaust needle, which can change the air pressure in the reaction part 20 to control the discharge of liquid in the reaction part 20. The exhaust needle is arranged in parallel with the magnetic component 30 and corresponds to each other, and then corresponds to the reaction tube 22, so as to facilitate the integrated design of the exhaust needle and the magnetic component 30, and the overall movement is synchronized to realize the exhaust needle into the reaction part 20 and the magnetic component 30 moving to the side wall of the reaction part 20 through the second hole 212, and then the magnetic unit 80 can be combined with the solution discharge operation, which simplifies the operation steps and improves the extraction efficiency.

[0054] Further, the bracket 60 is provided with a mounting part 61, and the liquid discharge device 50 and the magnetic component 30 are arranged in the mounting part 61, and the liquid discharge device 50 and the magnetic component 30 are arranged in an array corresponding to each other. When the bracket 60 moves, the liquid discharge device 50 can be driven to partially penetrate into the reaction part 20 to change the air pressure in the reaction part 20, and the magnetic component 30 can be driven to move to the side wall of the reaction tube 22 through the second hole 212 to apply a magnetic force. In the present embodiment, the liquid discharge device 50 and the magnetic component 30 are arranged in the mounting part 61, which can realize the synchronous extraction operation of a plurality of reaction tubes 22, improve the flux of the device, and reduce the workload of extracting polypeptides. In some embodiments, as shown, Figure 6 the liquid discharge device 50 is configured as a hollow tube structure, the magnetic component 30 is configured as a solid magnetic rod, and the liquid discharge device 50 and the magnetic component 30 are arranged in parallel in the mounting part 61.

[0055] In some embodiments, the base 10 is provided with a coarse adjustment switch 51 and a fine adjustment switch 52 for adjusting the exhaust amount of the exhaust needle, which further improves the precision of the operation and ensures the thoroughness and efficiency of the solid-liquid separation.

[0056] The polypeptide extraction device according to the embodiments of the present application is used for polypeptide extraction experiment, and the experimental process is as follows:

[0057] S1. Sample preparation: 100 μL of human serum sample is taken into each reaction tube 22, and 200 μL of polypeptide extraction buffer is added for dilution;

[0058] S2. Polypeptide adsorption: magnetic beads are added respectively, and incubated at room temperature for 1 hour to allow the polypeptides to be adsorbed to the surface of the magnetic beads;

[0059] S2. Magnetic bead washing: the magnetic bead-polypeptide complex is washed using a washing solution, the magnetic bead-polypeptide complex is adsorbed and fixed by the magnetic component 30, and the waste liquid containing unadsorbed protein impurities is quickly discharged to the waste liquid collecting part 40 by the air pressure of the liquid discharging device 50;

[0060] S3. Polypeptide elution: polypeptides are eluted using a 0.1M acid solution, the magnetic beads are adsorbed and fixed by the magnetic component 30, and the unadsorbed solution containing polypeptides is quickly discharged to the sample collecting part 40 by the air pressure of the liquid discharging device 50;

[0061] S4. Polypeptide quantification: polypeptides are detected and their content is determined using liquid chromatography-mass spectrometry-mass spectrometry.

[0062] Experimental results:

[0063] Compared with the polypeptide extraction device commonly used in the related art, the extraction efficiency of the polypeptide extraction device of the embodiment of the utility model is improved by 50%.

[0064] In the description of the utility model, it is understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential" is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the utility model and simplifying the description, and therefore cannot be understood as a limitation on the utility model.

[0065] In the description of the utility model, "first feature" and "second feature" can include one or more features.

[0066] In the description of the utility model, "a plurality of" means two or more.

[0067] In the description of the utility model, "above" or "below" of the first feature to the second feature can include that the first and second features are in direct contact, or can include that the first and second features are not in direct contact but are in contact through another feature between them.

[0068] In the description of the utility model, "above", "above" and "above" of the first feature to the second feature include that the first feature is directly above and obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature.

[0069] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "illustrative embodiment", "example", "specific example" or "some examples" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily mean the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0070] Although the embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the claims and their equivalents.

Claims

1. A polypeptide extraction device, characterized by, The utility model relates to a polypeptide extraction device, comprising: a base provided with a bearing platform; a reaction part provided on the bearing platform and adapted to accommodate sample solution and magnetic units; a magnetic component provided on the base and capable of selectively adsorbing the magnetic units; the magnetic component is movably provided on the base to selectively approach the magnetic units and adsorb the magnetic units or move away from the magnetic units to reduce the adsorption force on the magnetic units; the polypeptide extraction device further comprises: a guide rail provided on the base; a support movably provided on the guide rail to selectively approach or move away from the bearing platform, the magnetic component is provided on the support and is adapted to move synchronously with the support; the guide rail is provided on the bearing platform and extends on the surface of the bearing platform or is perpendicular to the bearing platform.

2. The polypeptide extraction device of claim 1, wherein, The magnetic component is configured as an electromagnet adapted to selectively generate or eliminate the adsorption force on the magnetic units.

3. The polypeptide extraction device of claim 1, wherein, Further comprising: a collection part provided on the bearing platform, the collection part is formed with a collection cavity adapted to collect the solution discharged from the reaction part; a liquid discharge device provided on the base, the liquid discharge device is adapted to drive the solution in the reaction part to be discharged into the collection cavity.

4. The polypeptide extraction device of claim 3, wherein, The liquid discharge device is provided on the support and selectively extends into the reaction part to change the air pressure in the reaction part to control the liquid discharge in the reaction part.

5. The polypeptide extraction device of claim 4, wherein, The liquid discharge device is provided with an exhaust outlet adapted to exhaust air into the reaction part to change the air pressure in the reaction part.

6. The polypeptide extraction device of claim 5, wherein, The reaction part comprises: a plurality of reaction tubes spaced apart on the bearing platform, each reaction tube is formed with a fitting part at one end away from the bearing platform, and the other end of the reaction tube is provided with a liquid discharge port; a well plate formed with a plurality of first holes and a plurality of second holes, each first hole corresponds to one of the fitting parts of the reaction tubes and is adapted to be passed through by the liquid discharge device to enter the reaction tube, and the second hole is adapted to be passed through by the magnetic component.

7. The polypeptide extraction device of claim 6, wherein, The liquid discharge device is configured as an exhaust needle, the exhaust needle is configured as a plurality of ones corresponding to the reaction tubes, each exhaust needle is adapted to extend into the reaction tube through the first hole; the magnetic component is configured as a plurality of ones corresponding to the exhaust needles, each magnetic component is provided in parallel with the exhaust needle and moves to the outer wall of the reaction tube through the corresponding second hole.

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