Automatic liquid feeding device of chromatographic column

Abstract

The utility model discloses an automatic liquid feeding device of a chromatographic column, which comprises a base, a clamping frame in the middle of the base, the chromatographic column arranged on the clamping frame, a collecting pipe arranged at the bottom of the chromatographic column, a solvent tank arranged at the top of the chromatographic column, a liquid inlet arranged on the solvent tank, an eluent tank arranged on the base, and an eluent cavity arranged at the top of the clamping frame. The eluent cavity is communicated with the eluent pool through a telescopic pipeline, the eluent cavity is provided with a liquid outlet, a communicating pipeline is arranged between the liquid outlet and the liquid inlet, the middle of the clamping frame is of a hollow structure, a lifting rod is arranged in the hollow structure, and a multi-section oil cylinder is arranged at the bottom of the lifting rod. According to the utility model, eluent can be sucked into the eluent cavity from the eluent tank through the pump body and finally flows into the solvent tank; during chromatography, an eluent flows into the chromatographic column from the solvent tank, and the solvent tank is kept at constant pressure, so that the automatic feeding of the eluent is realized; after chromatography is finished, the eluent cavity is lowered to the same height as the solvent pool through the multi-section oil cylinder, liquid feeding is stopped, and the device is ingenious in design, high in practicability and suitable for popularization.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of chromatography column, more specifically to an automatic liquid feeding device of chromatography column. BACKGROUND

[0002] The main principle of column chromatography technology is that components are separated by repeated distribution according to the different distribution coefficients of each component in the sample mixture in the stationary phase and the mobile phase. During column chromatography operation, an insoluble matrix is first filled in a cylindrical tube to form a stationary phase. The sample is added to the column and eluted with a special solvent, and the solvent constitutes the mobile phase. During the elution of the sample from the column, the components are separated by repeated distribution according to the different distribution coefficients of each component in the sample mixture in the stationary phase and the mobile phase. During the chromatography process, the chromatography material is often dripped into the collection tube from the bottom outlet of the chromatography column after chromatography, and the eluent flows into the chromatography column from the top, thus eluting the chromatography column. Therefore, the existing chromatography column usually has an eluent device at the top, but in actual use, the eluent is often insufficient, and the staff needs to manually add it, which is time-consuming and laborious, affecting the chromatography efficiency. To solve this problem, the present inventor proposes an automatic liquid feeding device for chromatography column, which adds an eluent automatic liquid feeding device to automatically add eluent, facilitates chromatography operation, improves chromatography efficiency, and reduces the labor intensity of the staff.

[0003] In order to realize the automatic feeding of chromatography liquid, facilitate the operation of the operator, and improve the chromatography efficiency, a chromatography column automatic liquid feeding device is disclosed in Chinese patent application No. CN213050486U. The utility model includes a chromatography column, a liquid storage bottle, a connecting hose, and a lifting frame. The liquid storage bottle has a liquid storage cavity and a communication cavity. A communication port is provided between the communication cavity and the liquid storage cavity. The cavity wall of the liquid storage cavity is provided with a vent hole and a liquid outlet. The chromatography column is provided with a liquid inlet pipe on the upper side and a vent hole. One end of the connecting hose is connected with the liquid outlet, and the other end is connected with the liquid inlet pipe. The lifting frame includes a fixed frame and a movable frame, and the liquid storage bottle is arranged on the movable frame. The liquid level in the chromatography column and the liquid level in the communication cavity are kept at the same height, achieving the purpose of automatic liquid feeding and maintaining the liquid level. The structure is simple and easy to operate.

[0004] The scheme still has some deficiencies in application, since the eluent needs to enter the top of the chromatography column during unloading, the eluent needs to be automatically fed, otherwise the chromatography will fail, and currently the eluent cavity is arranged on the base, and during the chromatography, the eluent needs to be sucked from the elution cavity to the elution solvent pool, and the structure is prone to the situation that the eluent cannot enter the solvent pool in time or in sufficient amount, thereby causing the chromatography to fail, therefore, the automatic feeding device of the eluent needs to be improved to realize automatic feeding, avoid insufficient feeding, and improve the success rate of the chromatography. Utility model content

[0005] The utility model discloses an automatic feeding device of chromatography column, which mainly aims at overcoming the above-mentioned deficiencies and shortcomings of the prior art.

[0006] The utility model employs the technical scheme as follows:

[0007] An automatic feeding device of chromatography column, including base, the middle of base is equipped with the clamping frame, and the chromatography column is correspondingly arranged on the clamping frame, and the chromatography column bottom is correspondingly equipped with the collection pipe, the chromatography column top is equipped with the solvent pool, and the solvent pool is equipped with the liquid inlet, one side of base is equipped with the eluent pool, the clamping frame top is equipped with the eluent cavity, and the eluent cavity and the eluent pool are communicated through the telescopic pipe, and the eluent cavity is equipped with the liquid outlet, and the communication pipe is arranged between the liquid outlet and the liquid inlet, and the clamping frame middle is hollow structure, and the hollow structure is built-in with the lifting rod, and the lifting rod bottom is equipped with the multi-section oil cylinder, and the multi-section oil cylinder can drive the lifting rod to go up and down.

[0008] Further, the eluent cavity is arranged on the top of the lifting rod, and the eluent cavity can go up and down through the multi-section oil cylinder.

[0009] Further, the eluent pool is built-in with the eluent and is equipped with the pump body, and the eluent is absorbed to the eluent cavity through the pump body.

[0010] Further, the base is equipped with the oil pressure station, and the oil pressure station is connected with the multi-section oil cylinder.

[0011] Further, the chromatography column top is equipped with the plug body, the solvent pool bottom is equipped with the insertion pipe, and the insertion pipe is inserted into the plug body and extends into the chromatography column.

[0012] Further, the chromatography column is built-in with the chromatography material, and the chromatography material top is equipped with the circular filter paper.

[0013] Further, the inside of the eluent cavity is equipped with the inductor.

[0014] Further, the front side of the base is equipped with the control panel.

[0015] Compared with the prior art, the utility model has the advantages that:

[0016] The utility model discloses first through the base setting eluent pool, and the top of clamping frame sets up eluent cavity simultaneously, and eluent can be absorbed from eluent pool to eluent cavity through pump body, and finally flow to solvent pool, then, the top of chromatographic column is equipped with plug body, and the insertion tube of solvent pool bottom inserts plug body and extends to chromatographic column, when chromatography, eluent flows to chromatographic column from solvent pool, and solvent pool keeps constant pressure, realizes the automatic liquid inlet of eluent through this setting, and the design is ingenious, and need not manual intervention, finally, when the eluent of eluent cavity is deficient, through inductor induction and through the control panel start pump body and carry out liquid supplement, after chromatography, through the oil cylinder of multiple sections and reduce eluent cavity to the same height with solvent pool, stop liquid inlet, the utility model discloses the design is ingenious, can realize the automatic liquid inlet of eluent, can also realize the opening and closing on demand simultaneously, and the automation level is high, and the practicality is strong, is fit for promoting. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is the front view structure schematic of the utility model Figure 1 .

[0018] Figure 2 It is the front view structure schematic of the utility model Figure 2 .

[0019] Figure 3 It is the top view structure schematic of the utility model. DETAILED DESCRIPTION

[0020] The specific implementation of the utility model will be further explained below with reference to the drawings.

[0021] As Figures 1 to 3 shown, an automatic liquid inlet device of chromatographic column, including base 1, the middle of base 1 is equipped with clamping frame 2, the clamping frame 2 is correspondingly equipped with chromatographic column 3, the bottom of chromatographic column 3 is correspondingly equipped with collection pipe 4, the top of chromatographic column 3 is equipped with solvent pool 5, the solvent pool 5 is equipped with liquid inlet 51, one side of base 1 is equipped with eluent pool 6, the top of clamping frame 2 is equipped with eluent cavity 7, the eluent cavity 7 and eluent pool 6 are communicated by telescopic pipe, the eluent cavity 7 is equipped with liquid outlet 71, and the liquid outlet 71 and liquid inlet 51 are equipped with communication pipe, the middle of clamping frame 2 is hollow structure, and the hollow structure is built-in lifting rod 8, the bottom of lifting rod 8 is equipped with multiple oil cylinders 9, and the multiple oil cylinders 9 can drive lifting rod 8 to go up and down.

[0022] Further, the eluent cavity 7 is arranged on the top of the lifting rod 8, and the eluent cavity 7 can be lifted up and down by the multiple oil cylinders 9.

[0023] Further, the eluent tank 6 is internally provided with eluent and is provided with a pump body 61, and the eluent is sucked into the eluent cavity 7 by the pump body 61.

[0024] Further, the base 1 is provided with an oil pressure station 10 connected with the multi-section oil cylinder 9.

[0025] Further, the top of the chromatographic column 3 is provided with a plug body 31, and the bottom of the solvent tank 5 is provided with a insertion pipe 52 inserted into the plug body 31 and extending into the chromatographic column 3.

[0026] Further, the chromatographic column 3 is internally provided with chromatographic material, and the top of the chromatographic material is provided with a circular filter paper 32.

[0027] Further, the inside of the eluent cavity 7 is provided with a sensor 72.

[0028] Further, the front side of the base 1 is provided with a control panel 11.

[0029] [Embodiment]

[0030] Firstly, the eluent is filled into the eluent tank 6, and the eluent is first injected into the eluent cavity 7 by the pump body 61, and then flows into the solvent tank 5, then during chromatography, the chromatographic material flows into the collection pipe 4 from the chromatographic column 3, and at the same time, the eluent flows into the chromatographic column 3 from the insertion pipe 52 of the solvent tank 5, so as to realize automatic liquid feeding of the eluent, and then when the eluent in the eluent cavity 7 is insufficient, the sensor 72 is first sensed, and then the pump body 61 is started by the control panel 11, and the eluent is continuously sucked from the eluent tank 6 into the eluent cavity 7, and finally when the chromatography is completed, the eluent cavity 7 is lowered to the same height as the solvent tank 5 by the multi-section oil cylinder 9, and the liquid feeding of the eluent is stopped.

[0031] From the above description of the utility model, compared with the prior art, the utility model has the advantages that:

[0032] The utility model firstly sets the eluent tank through the base, and the top of the clamping frame is provided with the eluent cavity, the eluent can be sucked into the eluent cavity from the eluent tank by the pump body, and finally flows into the solvent tank, then the top of the chromatographic column is provided with the plug body, the insertion pipe at the bottom of the solvent tank is inserted into the plug body and extends into the chromatographic column, during chromatography, the eluent flows into the chromatographic column from the solvent tank, the solvent tank keeps constant pressure, and the automatic liquid feeding of the eluent is realized through the setting, and the design is ingenious and does not need manual intervention, finally when the eluent in the eluent cavity is insufficient, the sensor is sensed and the pump body is started by the control panel to supplement the liquid, after the chromatography is completed, the eluent cavity is lowered to the same height as the solvent tank by the multi-section oil cylinder, and the liquid feeding is stopped, the utility model has the advantages of ingenious design, automatic liquid feeding of the eluent, on-demand opening and closing, high automation level, strong practicability and suitability for promotion.

[0033] The above merely illustrates the specific embodiments of the present application, but the design concept of the present application is not limited to this, and any non-substantial improvement of the present application by using the concept should belong to the act of infringing the protection scope of the present application.

Claims

1. An automatic liquid feeding device for a chromatographic column, characterized by: The application relates to a chromatography device, which comprises a base, a clamping frame arranged in the middle of the base, a chromatography column arranged on the clamping frame, a collecting pipe arranged at the bottom of the chromatography column, a solvent pool arranged at the top of the chromatography column, a liquid inlet arranged on the solvent pool, an eluent pool arranged on one side of the base, an eluent cavity arranged on the top of the clamping frame, an eluent pool and the eluent cavity being communicated through an elastic pipe, a liquid outlet arranged on the eluent cavity, a communication pipe arranged between the liquid outlet and the liquid inlet, a hollow structure arranged in the middle of the clamping frame, a lifting rod arranged in the hollow structure, a multi-section oil cylinder arranged at the bottom of the lifting rod, and the multi-section oil cylinder being capable of driving the lifting rod to move up and down.

2. The automatic liquid feeding device for chromatographic column according to claim 1, characterized in that: The eluent cavity is arranged on the top of the lifting rod, and the eluent cavity is capable of moving up and down through the multi-section oil cylinder.

3. The automatic liquid feeding device for chromatographic column according to claim 1, characterized in that: The eluent pool is arranged in the base, and the eluent pool is arranged with a pump body and is filled with eluent, and the eluent is capable of being pumped to the eluent cavity through the pump body.

4. The automatic liquid feeding device for chromatographic column according to claim 1, characterized in that: The base is arranged with an oil pressure station, and the oil pressure station is connected with the multi-section oil cylinder.

5. The automatic liquid feeding device for chromatographic column according to claim 1, characterized in that: The top of the chromatography column is arranged with a plug, the bottom of the solvent pool is arranged with a pipe, the pipe is inserted into the plug, and the pipe extends into the chromatography column.

6. The automatic liquid feeding device for chromatographic column according to claim 1, characterized in that: The chromatography column is arranged with chromatography material, and the chromatography material is arranged with a circular filter paper at the top.

7. The automatic liquid feeding device for chromatographic column according to claim 1, characterized in that: The eluent cavity is arranged with a sensor.

8. The automatic liquid feeding device for chromatographic column according to claim 1, characterized in that: The front side of the base is arranged with a control panel.

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