Environment-friendly multi-joint detection chromatography card box

Abstract

The utility model discloses an environment-friendly multi-joint detection chromatography card box, the card box is formed by bonding a chip bottom plate and a substrate, and the lower end face of the chip bottom plate is provided with at least more than one reaction area, an inwards sunken liquid separation area and air holes; the sample adding hole is formed in the chip bottom plate, penetrates through the liquid separation area and is used for adding a sample into the liquid separation area; one end of the reaction zone is communicated with the liquid separation zone, and the other end of the reaction zone is communicated with the air hole; a chromatography strip is placed on the upper end surface of the base plate and is positioned in the corresponding reaction area. According to the utility model, the substrate is provided with a plurality of reaction areas, multi-joint detection application can be realized, and after the substrate made of glass is adopted, due to the natural hydrophilicity of the substrate, a hydrophilic film does not need to be used, so that the problem that a film layer falls off or is polluted is avoided, and the accuracy of a detection result and the long-term stability of a system are ensured.

Description

TECHNICAL FIELD

[0001] The utility model relates to microfluidic technology and chromatography detection technical field, concretely is a kind of environmental friendly multi-association inspection chromatography card box. BACKGROUND

[0002] With the increasing demand for efficient analysis and detection in the fields of biological medicine, environmental monitoring and food safety, traditional separation and analysis techniques face many challenges, especially in terms of analysis speed, resolution, automation level and multi-component detection. Chromatography technology, as a classic separation technology, has been widely used in component separation, purification and quantitative analysis of samples. However, traditional chromatography technology usually has the limitations of complicated operation, long analysis time and inability to handle multiple components simultaneously, especially when facing complex samples, these limitations are more obvious.

[0003] In recent years, with the development of microfluidic chip technology, chromatography technology based on microfluidic chip has gradually become a new solution. Chromatography chip can realize fast and efficient separation through micro-scale channels, and has low sample and reagent consumption. However, existing chromatography chips are mostly single analysis mode, usually only for analysis of a certain specific target substance. In actual application, especially in multi-component analysis and detection of complex samples, multiple separation or multi-step operation is often required, which not only increases the analysis period, but also reduces the experimental efficiency.

[0004] In order to meet the needs of rapid, accurate and diversified detection, researchers have proposed multiplex detection technology, which greatly improves the detection efficiency and reduces the cost by detecting multiple target substances simultaneously. However, existing chromatography chips have not yet been able to realize the simultaneous detection of multiple substances, especially the efficient and accurate multi-component detection on the same chip is still a technical problem. In addition, current multiplex microfluidic chips generally rely on plastic, silicon and other synthetic materials in terms of material selection and design. Although these materials have certain processing advantages, they often have a series of problems in use, especially in terms of liquid separation, surface hydrophilicity and operation simplicity. Traditional microfluidic chips usually need to add additional hydrophilic film layer to realize better liquid flowability and dispensing accuracy, but these additional film layers not only increase the manufacturing complexity, but also may introduce additional cost, pollution and operation error.

[0005] Therefore, developing an environmentally friendly chromatography chip that can perform multiplex analysis on a single platform has become an important research direction in the fields of biological analysis, clinical diagnosis and environmental monitoring. SUMMARY

[0006] The utility model discloses a kind of environment-friendly multi-association inspection chromatography card boxes, to solve the problems presented in the above background art.

[0007] To achieve the above object, the utility model provides the following technical scheme:

[0008] A kind of environment-friendly multi-association inspection chromatography card box, the card box is bonded by chip base plate and glass substrate, wherein:

[0009] The lower end surface of the chip base plate has more than two reaction zones, an inwardly recessed distribution zone, and air holes;

[0010] The sample addition hole is provided on the chip base plate and penetrates to the distribution zone, for adding sample to the distribution zone;

[0011] One end of the reaction zone is in communication with the distribution zone, and the other end of the reaction zone is in communication with the air hole.

[0012] The chromatography strip is located in the corresponding reaction zone.

[0013] Further, the adjacent reaction zones are separated by a barrier, and the outer end surface of the barrier is flush with the lower end surface of the chip base plate.

[0014] Further, the chromatography strip is placed on the substrate by a limiting component.

[0015] Further, the limiting component includes L-shaped stoppers clamped at the four corners of the chromatography strip, and the four L-shaped stoppers and the upper end surface of the substrate form a placement area for accommodating the chromatography strip.

[0016] Further, the chip base plate and the substrate are relatively fixed by a pre-positioning component.

[0017] Further, the pre-positioning component includes a column with specific elasticity and a positioning block, the column is installed at one end of the upper end surface of the substrate, and a anti-disengagement part is installed, the positioning block is installed at one end of the lower end surface of the chip base plate and away from the column, the lower end surface of the chip base plate has a insertion hole for the column and the anti-disengagement part, and the upper end surface of the substrate has an insertion slot for the positioning block.

[0018] Further, the anti-disengagement part is in the shape of a truncated cone, the hole bottom depth of the insertion hole is greater than the length of the anti-disengagement part, and the hole bottom is used for moving the anti-disengagement part in the axial direction.

[0019] Further, the glass material of the substrate is quartz glass or soda-lime glass.

[0020] Further, the distribution zone has a plurality of support structures.

[0021] Further, the shape of the sample adding hole is circular or square rectangle, and the shape is funnel-shaped, which is used for preventing sample from remaining on the sample adding hole.

[0022] Compared with the prior art, the utility model has the advantages of:

[0023] 1. The glass substrate has natural hydrophilicity, simplifying the liquid separation process: The glass itself has good hydrophilicity, which can naturally guide the liquid to flow in the flow channel without additional hydrophilic film or coating. This hydrophilicity allows the injected sample to flow smoothly into each reaction zone, achieving uniform liquid separation without increasing additional process complexity. By directly utilizing the hydrophilicity of the glass substrate, the production process can be significantly simplified, costs can be reduced, and the reliability of the system can be improved.

[0024] 2. Avoiding contamination risk introduced by hydrophilic film: In traditional microfluidic chips, hydrophilic film is usually treated by chemical coating or thin film technology, which may introduce additional sources of contamination, especially when handling biological samples. The aging or shedding of the film layer may cause contamination. However, by using a glass substrate, the natural hydrophilicity eliminates the need for a hydrophilic film, thereby avoiding the problem of film shedding or contamination, ensuring the accuracy of the test results and the long-term stability of the system.

[0025] 3. Improve liquid separation accuracy and reduce errors: The chip bottom plate has multiple liquid separation zones as flow channels. Due to the good wettability of the glass substrate surface, the injected sample can flow stably along the flow channel, ensuring the accuracy of liquid distribution in different detection projects. This precise liquid separation capability is particularly important in multi-association detection applications, especially when multiple tests are performed, ensuring accurate liquid distribution in each flow channel, reducing errors caused by uneven liquid distribution, and improving the reliability and accuracy of the test.

[0026] 4. Reduce production complexity and cost: Since the glass substrate itself has hydrophilicity, the complex hydrophilic film processing step can be omitted, reducing material and process investment. This not only simplifies the production process, but also reduces production costs, promoting the widespread application of multi-association microfluidic chips. Compared to traditional chips that require multiple process steps, the hydrophilic design of the glass substrate makes the entire production process more efficient and economical.

[0027] 5. Enhance the long-term stability of the chip: The chemical stability and high temperature resistance of the glass substrate make the chip less susceptible to external environmental factors during long-term use, and the performance will not decrease due to temperature, humidity changes or ultraviolet radiation. Therefore, the glass substrate chip has a longer service life in high-precision detection, reducing the need for replacement and maintenance. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 It is the structure schematic view of the embodiment 1 of the utility model.

[0029] Figure 2 It is the base plate and chip bottom plate separation schematic view of the embodiment 1 of the utility model.

[0030] Figure 3 It is another visual angle schematic view of the utility model. Figure 2

[0031] Figure 4 It is the structure schematic view of the embodiment 2 of the utility model.

[0032] Figure 5 It is the structure schematic view of the embodiment 3 of the utility model.

[0033] Figure 6 It is another visual angle schematic view of the utility model. Figure 5

[0034] Figure 7 It is the base plate and chip bottom plate through column body rotation certain angle schematic view of the embodiment 3 of the utility model.

[0035] In the drawing: 1-chip bottom plate, 2-base plate, 3-sample hole, 4-liquid separation area, 5-reaction area, 6-barrier part, 7-air hole, 8-chromatography strip, 9-stop block, 10-column body, 11-anti-drop part, 12-jack, 13-slot, 14-, 15-supporting structure, 16-hole bottom. DETAILED DESCRIPTION

[0036] The technical scheme in the embodiments of the utility model will be described clearly and completely in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by the person skilled in the art without creative labor belong to the protection scope of the utility model.

[0037] In the description of the utility model, it is necessary to explain that the orientation or position relation indicated by the terms "upper end", "lower end", "inner", "outer", "front end", "rear end", "two ends", "one end", "another end" and the like is the orientation or position relation shown in the drawing, and is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, so it cannot be understood as a limitation on the utility model. In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0038] ​​In the description of the utility model, it is necessary to explain that, unless there is definite stipulation and limitation, the terms "mount", "be provided with", "be provided with", "be provided with", "connect" and the like should be understood in a broad sense, for example, "connect", can be fixedly connected, also can be detachably connected, or integrally connected;Can be mechanical connection, also can be electrical connection;Can be directly connected, also can be indirectly connected through intermediate medium, can be the communication inside two elements.For the ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to specific circumstances.

[0039] Embodiment 1

[0040] Please refer to Figures 1 to 3 The utility model provides a technical scheme:

[0041] A kind of environmental friendly multi-connection test chromatography card box, the card box is bonded by chip base plate 1 of plastic material such as PMMA (acrylic) and substrate 2 of glass material, wherein:

[0042] The lower end surface of the chip base plate 1 has more than two reaction zones 5, an inwardly recessed liquid separation zone 4 and an air hole 7;

[0043] Sample well 3 is opened on chip base plate 1 and penetrates to liquid separation zone 4, for adding sample to liquid separation zone 4;

[0044] One end of the reaction zone 5 is in communication with the liquid separation zone 4, and the other end of the reaction zone 5 is in communication with the air hole 7;

[0045] The upper end surface of the substrate 2 is placed with a chromatography strip 8, and the chromatography strip 8 is located in the corresponding reaction zone 5, and the upper end surface and the lower end surface are based Figures 1 to 3 View angle.

[0046] Specifically, adjacent reaction zones 5 are separated by a barrier 6, and the outer end surface of the barrier 6 is flush with the lower end surface of the chip base plate 1.

[0047] Specifically, the glass material of the substrate 2 is quartz glass or soda-lime glass.

[0048] Specifically, the liquid separation zone 4 has a plurality of support structures 15.

[0049] Specifically, the shape of the sample well 3 is circular or square rectangular, in the form of a funnel, for preventing sample residue on the sample well 3.

[0050] The environment-friendly multi-association detection chromatography card box of the embodiment is a multi-association detection chromatography chip based on chromatography principle. The environment-friendly multi-association detection chromatography card box is mainly applied to the fields of biological analysis, environmental monitoring, clinical diagnosis, food safety detection and the like, and belongs to the cross field of analytical chemistry, microfluidic chip technology and separation science. Specifically, the card box can be widely applied to multi-component simultaneous detection and analysis, especially in scenarios requiring high-throughput, rapid and accurate analysis, such as large-scale sample screening, qualitative and quantitative analysis of complex sample components and the like.

[0051] The environment-friendly multi-association detection chromatography card box not only should have high separation performance, but also should have highly integrated design, so as to realize multi-component simultaneous separation, detection and quantitative analysis in the chip to meet the application requirements of environmental protection, diversification, high efficiency and accuracy.

[0052] Specifically, the chip bottom plate 1 made of glass material is used as the base material of the microfluidic chip, and the natural hydrophilicity is fully utilized, so that the liquid separation process is simplified, the pollution and cost problems caused by the traditional hydrophilic membrane are avoided, the liquid separation precision is improved, the operation error is reduced, and the performance, stability and economy of the multi-association detection chip are significantly improved. Specifically, the multi-association detection card box made of glass material in the embodiment is formed by bonding the chip bottom plate 1 and the substrate 2, a plurality of reaction zones 5 are integrated in the card box, and the chromatography strip 8 is located in the reaction zone 5, thereby constituting an independent detection module, and high-efficiency detection of different components can be realized, thereby providing a new solution for solving the multi-component analysis bottleneck in the prior art.

[0053] In the embodiment, the chip bottom plate 1 is provided with a sample adding hole 3, a liquid separation zone 7, and a reaction zone 5 and an air hole 7.

[0054] The shape of the sample adding hole 3 can be circular or square rectangular, and is in the form of a funnel to prevent sample residue.

[0055] The liquid separation zone 4 and the sample adding hole 3 are connected by a wide flow channel with a support structure 15, and the wide flow channel directly separates downstream, which can be 2-way, 3-way, 4-way or more, that is, the reaction zone 5 can have 2, 3, 4 or more. This separation method can reduce the separation error caused by flow resistance. It should be noted that the wide flow channel is formed due to the concave of the liquid separation zone 4, and the liquid separation zone 4 has a spacing from the lower end surface of the chip bottom plate 1. The support structure 15 is more specifically a plurality of protrusions.

[0056] The chromatography strip 8 is placed in the reaction zone 5, and the reaction zone 5 is not limited to 3 (3-association detection) as shown in the embodiment, but can be further divided into 4-association detection, 5-association detection and the like. Figure 3

[0057] The substrate 2 as the upper cover is a glass substrate, and the glass itself has hydrophilicity, which is convenient for liquid separation. The material can be quartz glass, soda-lime glass and the like.​

[0058] Assembling: the treated chromatographic strip 8 is placed in the flow channel of the reaction area 5, and then the chip base plate 1 and the substrate 2 are bonded by glue or double-sided tape, which is lower in cost and simpler in assembling than the conventional film pasting or ultrasonic bonding of the chip.

[0059] Specific detection process:

[0060] 1. 200 microliters of sample is taken and added to the sample well 3.

[0061] 2. The sample flows through the separation area 4 into the chromatographic strip 8 in the reaction area 5.

[0062] 3. The sample continues to flow forward on the chromatographic strip 8 by capillary force.

[0063] 4. After 10 minutes of reaction, the color development of the T line and the C line (not shown in the figure) of the chromatographic strip 8 is observed, if only the C line is positive, if both T and C are positive, if neither the C line nor the T line is positive, the chromatographic strip 8 is invalid.

[0064] 5. Multi-association detection relies on the reagents coated on the chromatographic strip, and different reagents can be coated to realize multi-association detection.

[0065] Embodiment 2

[0066] Please refer to Figure 4 The utility model provides a technical scheme, on the basis of embodiment 1, the following contents are increased:

[0067] The chromatographic strip 8 is placed on the substrate 2 through the limiting component.

[0068] The limiting component includes the L-shaped stopper 9 clamped at the four corners of the chromatographic strip 8, and the four L-shaped stoppers 9 and the upper end surface of the substrate 2 form a placement area for accommodating the chromatographic strip 8.

[0069] Each chromatographic strip 8 corresponds to four stoppers 9, and the stopper 9 is installed on the upper end surface of the substrate 2.

[0070] If there is a process or installation requirement that the chromatographic strip 8 is placed on the substrate 2 first and then the chip base plate 1 is covered on the substrate 2, the chromatographic strip 8 can be placed on the substrate 2.

[0071] When the chromatographic strip 8 is placed on the substrate 2 and the chromatographic strip 8 is not fixed on the substrate 2 by gluing or pasting, the chromatographic strip 8 may move on the substrate 2 or fall off the substrate 2 due to vibration.

[0072] Therefore, the stopper 9 and the placement area formed by the stopper 9 and the like can be used to clamp the chromatographic strip 8 into the placement area, so that the chromatographic strip 8 is relatively fixed, and movement of the chromatographic strip 8 on the substrate 2 or falling of the chromatographic strip 8 from the substrate 2 is avoided. The chip substrate 1 and the substrate 2 are bonded, and the chromatographic strip 8 is fixed.

[0073] Embodiment 3

[0074] Please refer to Figures 5 to 7 , the utility model provides a technical scheme, on the basis of embodiment 1 has increased following content:

[0075] The chip substrate 1 and the substrate 2 are relatively fixed by the pre-positioning component.

[0076] The pre-positioning component includes a column 10 with specific elasticity and a positioning block 14, and the material is medical silica gel, silica gel or PE and the like. The column 10 is installed at one end of the upper end surface of the substrate 2, and is provided with an anti-falling part 11. The positioning block 14 is installed at one end of the lower end surface of the chip substrate 1 and is away from the column 10. The lower end surface of the chip substrate 1 has a plug hole 12 for the column 10 and the anti-falling part 11 to be inserted. The upper end surface of the substrate 2 has a plug slot 13 for the positioning block 14 to be inserted.

[0077] The anti-falling part 11 is in the shape of a circular truncated cone. The depth of the hole bottom 16 of the plug hole 12 is greater than the length of the anti-falling part 11. The hole bottom 16 is used for the anti-falling part 11 to move in the axial direction.

[0078] In this embodiment, when the chip substrate 1 and the substrate 2 are bonded by using glue or double-sided adhesive tape, the four edges of the chip substrate 1 and the substrate 2 need to be aligned and covered together. In this process, the four edges need to be carefully aligned, and a slight carelessness can easily cause misalignment. After alignment, if there is a slight vibration, misalignment may occur between the chip substrate 1 and the substrate 2. Therefore, the pre-positioning component is used to pre-fix one end of the chip substrate 1 and the substrate 2, as shown in the figure. Since there is the column 10 between the chip substrate 1 and the substrate 2, the chip substrate 1 and the substrate 2 can be rotated and opened to a certain angle, as shown in the figure. Then, the treated chromatographic strip 8 is placed into the flow channel of the reaction area 5 (the chromatographic strip 8 can also be placed into the reaction area 5 in advance, and then pre-fixed). The glue or double-sided adhesive tape is coated or pasted on the chip substrate 1 or the substrate 2. Finally, the chip substrate 1 is rotated relative to the substrate 2 in the direction of the arrow A in the figure, so that the chip substrate 1 and the substrate 2 are overlapped. After the positioning block 14 is inserted into the plug slot 13, it is indicated that the four edges of the chip substrate 1 and the substrate 2 are aligned, the alignment and covering are achieved, and the chip substrate 1 and the substrate 2 are bonded. Figure 7 Figure 7

[0079] As shown in the figure Figure 5 and Figure 6 ​​As shown, in this embodiment, because the maximum diameter of the anti-disengagement portion 11 is slightly larger than the diameter of the column 10, and the anti-disengagement portion 11 is elastic, the anti-disengagement portion 11 can be inserted into the insertion hole 12 and then finally into the hole bottom 16, and the diameter of the hole bottom 16 is the same as the maximum diameter of the anti-disengagement portion 11, and the anti-disengagement portion 11 can move a certain distance along the axial direction of the hole bottom 16, so that the anti-disengagement portion 11 will not disengage from the hole bottom 16 in the case that the chip bottom plate 1 and the substrate 2 are not pulled by a very large external force. Because the anti-disengagement portion 11 is inserted into the hole bottom 16, the pre-fixing between the chip bottom plate 1 and the substrate 2 can be achieved.

[0080] When the chip bottom plate 1 and the substrate 2 are pre-fixed, the four edges of the chip bottom plate 1 and the substrate 2 are not necessarily completely aligned, that is, there is a certain angle between the chip bottom plate 1 and the substrate 2, at which time the chip bottom plate 1 and the substrate 2 can be relatively rotated, and because the positioning block 14 has a certain height and protrudes outward from the lower end surface of the chip bottom plate 1, the depth of the hole bottom 16 is greater than the length of the anti-disengagement portion 11 and the column 10 is also elastic, so that the chip bottom plate 1 or the substrate 2 can be slightly bent outward, and this embodiment takes the chip bottom plate 1 as an example, that is, it is slightly lifted along the direction of arrow B, so that the chip bottom plate 1 and the substrate 2 have a certain gap at one end of the positioning block 14, so that when the chip bottom plate 1 and the substrate 2 are relatively rotated, the positioning block 14 can be easily inserted into the insertion slot 13. Figure 7

[0081] The part not described in the utility model is the existing or known technology.

[0082] Although the embodiments of the utility model have been shown and described, it can be understood by those skilled in the art that various changes, modifications, replacements and variations can be made to these embodiments without departing from the principles and spirits of the utility model, and the scope of the utility model is defined by the appended claims and their equivalents.​

Claims

1. An environmentally friendly multi-stage chromatography cartridge, characterized in that, The card box is bonded by a chip base plate (1) and a glass substrate (2), wherein: The lower end surface of the chip base plate (1) has more than two reaction zones (5), an inwardly recessed distribution zone (4) and an air hole (7); A sample hole (3) is arranged on the chip base plate (1) and penetrates the distribution zone (4), and is used for adding a sample to the distribution zone (4); One end of the reaction zone (5) is communicated with the distribution zone (4), and the other end of the reaction zone (5) is communicated with the air hole (7); A chromatographic strip (8) is arranged in the corresponding reaction zone (5).

2. The environmentally friendly multi-project simultaneous detection chromatography cassette according to claim 1, wherein, The adjacent reaction zones (5) are separated by a barrier (6), and the outer end surface of the barrier (6) is flush with the lower end surface of the chip base plate (1).

3. The environmentally friendly multi-project simultaneous detection chromatography cassette of claim 1, wherein, The chromatographic strip (8) is placed on the substrate (2) by a limiting component.

4. The environmentally friendly multi-project simultaneous detection chromatography cassette of claim 3, wherein, The limiting component includes L-shaped stoppers (9) clamped at four corners of the chromatographic strip (8), and four L-shaped stoppers (9) and the upper end surface of the substrate (2) form a placement area for accommodating the chromatographic strip (8).

5. The environmentally friendly multi-project simultaneous detection chromatography cassette of claim 1, wherein, The chip base plate (1) and the substrate (2) are relatively fixed by a pre-positioning component.

6. The environmentally friendly multi-project simultaneous detection chromatography cassette of claim 5, wherein, The pre-positioning component includes a column (10) with specific elasticity and a positioning block (14), the column (10) is installed at one end of the upper end surface of the substrate (2) and is provided with an anti-disengagement part (11), the positioning block (14) is installed at one end of the lower end surface of the chip base plate (1) and is away from the column (10), the lower end surface of the chip base plate (1) has a jack (12) for inserting the column (10) and the anti-disengagement part (11), and the upper end surface of the substrate (2) has a slot (13) for inserting the positioning block (14).

7. The environmentally friendly multi-project simultaneous detection chromatography cassette of claim 6, wherein, The anti-disengagement part (11) is in the shape of a circular truncated cone, the depth of a hole bottom (16) of the jack (12) is greater than the length of the anti-disengagement part (11), and the hole bottom (16) is used for moving the anti-disengagement part (11) in the axial direction.

8. The environmentally friendly multi-project simultaneous detection chromatography cassette of claim 1, wherein, The glass material of the substrate (2) is quartz glass or soda-lime glass.

9. The environmentally friendly multi-project simultaneous detection chromatography cassette of claim 1, wherein, The distribution zone (4) has a plurality of support structures (15).

10. The environmentally friendly multi-project simultaneous detection chromatography cassette of claim 1, wherein, The sample hole (3) is in the shape of a circle or a square rectangle and is in the shape of a funnel, and is used for preventing the sample from remaining on the sample hole (3).

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